2as0

Crystal Structure of PH1915 (APC 5817): A Hypothetical RNA Methyltransferase

Method: X-RAY DIFFRACTION Dmax: 89.7 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

SAXS profile for 2as0

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2as0
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2as0
沉积日期 deposition_date2005-08-22
结构标题 titleCrystal Structure of PH1915 (APC 5817): A Hypothetical RNA Methyltransferase
关键词 keywords;RNA methyltransferase, Structural Genomics, PSI, Protein Structure Initiative, Midwest Center for Structural Genomics, MCSG, TRANSFERASE ;; TRANSFERASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier29.19
回转半径 Rg (电子) rg_electron28.27
零角强度 I(0) i0130984000.00
分子量 molecular_weight90572.0 kDa
排除体积 excluded_volume113220 ų
包络体积 envelope_volume137090 ų
水化壳体积 shell_volume39548 ų
包络直径 envelope_diameter93.9
壳层 Rg shell_rg36.35
包络 Rg envelope_rg28.16
形状 Rg shape_rg28.26
总 Rg total_rg29.07
总原子数 total_atoms6270
残基数 n_residues764
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax89.7
Rg (实空间) rg_real29.08
Rg 误差 (实空间) rg_real_error0.72
I(0) (实空间) i0_real1.3100e+08
I(0) 误差 (实空间) i0_real_error2.0650e+06
Rg (倒空间) rg_reciprocal29.13
I(0) (倒空间) i0_reciprocal131000000.0000
解质量估计 total_estimate0.9047
解质量评级 solution_quality EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary33.2
偏度 Skewness skewness0.215
峰度 Kurtosis kurtosis-0.476
角度范围 angular_range— – 0.2700 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha28080000.0000
实空间数据点数 n_real_points55
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.947; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.925

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (2)

6. 折叠分类 (SCOP + CATH) 10 domains

SCOP 2.08 (4 domains)

结构域编号 domain_idd2as0a1
类 Class classb — All beta proteins
折叠类型 Fold foldb.122 — PUA domain-like
超家族 Superfamily superfamilyb.122.1 — PUA domain-like
家族 Family familyb.122.1.9 — Hypothetical RNA methyltransferase domain (HRMD)
结构域编号 domain_idd2as0a2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.66 — S-adenosyl-L-methionine-dependent methyltransferases
超家族 Superfamily superfamilyc.66.1 — S-adenosyl-L-methionine-dependent methyltransferases
家族 Family familyc.66.1.51 — hypothetical RNA methyltransferase
结构域编号 domain_idd2as0b1
类 Class classb — All beta proteins
折叠类型 Fold foldb.122 — PUA domain-like
超家族 Superfamily superfamilyb.122.1 — PUA domain-like
家族 Family familyb.122.1.9 — Hypothetical RNA methyltransferase domain (HRMD)
结构域编号 domain_idd2as0b2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.66 — S-adenosyl-L-methionine-dependent methyltransferases
超家族 Superfamily superfamilyc.66.1 — S-adenosyl-L-methionine-dependent methyltransferases
家族 Family familyc.66.1.51 — hypothetical RNA methyltransferase

CATH v4.4 (6 domains)

结构域编号 domain_id2as0A01
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology130 — Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4
同源超家族 H-superfamily homologous superfamily10 — PUA domain
结构域编号 domain_id2as0A02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology750 — Transcription Regulator spoIIAA
同源超家族 H-superfamily homologous superfamily80 — RNA methyltransferase domain (HRMD) like
结构域编号 domain_id2as0A03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily150 — Vaccinia Virus protein VP39
结构域编号 domain_id2as0B01
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology130 — Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4
同源超家族 H-superfamily homologous superfamily10 — PUA domain
结构域编号 domain_id2as0B02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology750 — Transcription Regulator spoIIAA
同源超家族 H-superfamily homologous superfamily80 — RNA methyltransferase domain (HRMD) like
结构域编号 domain_id2as0B03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily150 — Vaccinia Virus protein VP39

7. 引用文献 (1)

8. 文件与曲线 (10)