2ba1

Archaeal exosome core

Method: X-RAY DIFFRACTION D_max: 112.7 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	2ba1
沉积日期 deposition_date	2005-10-13
结构标题 title	Archaeal exosome core
关键词 keywords	;EXOSOME, RNASE PH, RNA DEGRADATION, EXORIBONUCLEASE, RNA BINDING, S1 DOMAIN, ZN-RIBBON, ARCHAEAL, PHOSPHOROLYTIC, RNA BINDING PROTEIN ;; RNA BINDING PROTEIN
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	38.86 Å
回转半径 Rg (电子) rg_electron	37.79 Å
零角强度 I(0) i0	747745000.00
分子量 molecular_weight	223490.0 kDa
排除体积 excluded_volume	280390 Å³
包络体积 envelope_volume	381450 Å³
水化壳体积 shell_volume	79256 Å³
包络直径 envelope_diameter	114.1 Å
壳层 Rg shell_rg	47.66 Å
包络 Rg envelope_rg	36.68 Å
形状 Rg shape_rg	37.78 Å
总 Rg total_rg	38.39 Å
总原子数 total_atoms	15657
残基数 n_residues	2013
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	112.7 Å
Rg (实空间) rg_real	38.43 Å
Rg 误差 (实空间) rg_real_error	0.41 Å
I(0) (实空间) i0_real	7.4770e+08
I(0) 误差 (实空间) i0_real_error	1.0050e+07
Rg (倒空间) rg_reciprocal	38.70 Å
I(0) (倒空间) i0_reciprocal	747900000.0000
解质量估计 total_estimate	0.8980
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	55.0 Å
偏度 Skewness skewness	-0.077
峰度 Kurtosis kurtosis	-0.600
角度范围 angular_range	— – 0.2050 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	138100000.0000
实空间数据点数 n_real_points	42
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.930; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.952; Smooth: 0.929

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (5)

Entity 1 polymer

描述 description	Archaeal exosome RNA binding protein CSL4
分子量 formula_weight	19625.6 kDa
来源方法 src_method	man
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,C,B
原子数 atom_count	15740

MRFVMPGDRIGSAEEYVKGEGVYEEGGELFAAVAGKLIIKDRVAKVESISPIPEIVKGDVVLGRVVDLRNSIALIEVSSKKGENRGPSNRGIGILHVSNVDEGYVKEISEAVGYLDILKARVIGDNLRLSTKEEEMGVLRALCSNCKTEMVREGDILKCPECGRVEKRKISTDYGKGEW

来源 Source

来源生物 organism	Archaeoglobus fulgidus
NCBI 分类编号 ncbi_taxonomy_id	2234
宿主生物 host_organism	Escherichia coli

Entity 2 polymer

描述 description	Archaeal exosome complex exonuclease RRP41
分子量 formula_weight	28888.3 kDa
EC 编号 ec_number	3.1.13.-
来源方法 src_method	man
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E,D,F

MSEFNEKPEKLIVDGLRLDGRKFDELRPIKIEASVLKRADGSCYLEMGKNKVIAAVFGPREVHPRHLQDPSKAIIRYRYNMAPFSVEERKRPGPDRRSIEISKVSKEAFEAVIMKELFPRSAIDIFVEVLQADAGSRTACLNAASVALVDAGVPMKGMITSVAVGKADGQLVLDPMKEEDNFGEADMPFAFLIRNGKIESIALLQMDGRMTRDEVKQAIELAKKGALQIYEMQREAILRRYIEVGEEMDEITEGGEDA

来源 Source

来源生物 organism	Archaeoglobus fulgidus
NCBI 分类编号 ncbi_taxonomy_id	2234
宿主生物 host_organism	Escherichia coli

Entity 3 polymer

描述 description	Archaeal exosome complex exonuclease RRP42
分子量 formula_weight	28677.6 kDa
EC 编号 ec_number	3.1.13.-
来源方法 src_method	man
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H,G,I

MPEDILVDIKRDYVLSKLRDNERIDGRGFDEFRKVEIIPNVIEKAEGSALVKLGDTQVVVGVKMQPGEPYPDTPDRGVIIVNAELVPLASPTFEPGPPDENSIELARVVDRGIRESEAVDLSKLVIEEGEKVWIVFVDIHALDDDGNLLDASALAAIAALMNTKVPAERFDLGEDYLLPVRDLPVSVTSLIVGNKYLVDPSREEMSVGDTTLTITTDKDDNVVAMQKSGGYLLDEKLFDELLDVSINCARKLREKFKEI

来源 Source

来源生物 organism	Archaeoglobus fulgidus
NCBI 分类编号 ncbi_taxonomy_id	2234
宿主生物 host_organism	Escherichia coli

Entity 4 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

Entity 5 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	83

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6. 折叠分类 (SCOP + CATH) 27 domains

SCOP 2.08 (12 domains)

结构域编号 domain_id	d2ba1d1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.4 — Ribonuclease PH domain 1-like

结构域编号 domain_id	d2ba1d2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.101 — Ribonuclease PH domain 2-like
超家族 Superfamily superfamily	d.101.1 — Ribonuclease PH domain 2-like
家族 Family family	d.101.1.1 — Ribonuclease PH domain 2-like

结构域编号 domain_id	d2ba1e1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.4 — Ribonuclease PH domain 1-like

结构域编号 domain_id	d2ba1e2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.101 — Ribonuclease PH domain 2-like
超家族 Superfamily superfamily	d.101.1 — Ribonuclease PH domain 2-like
家族 Family family	d.101.1.1 — Ribonuclease PH domain 2-like

结构域编号 domain_id	d2ba1f1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.4 — Ribonuclease PH domain 1-like

结构域编号 domain_id	d2ba1f2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.101 — Ribonuclease PH domain 2-like
超家族 Superfamily superfamily	d.101.1 — Ribonuclease PH domain 2-like
家族 Family family	d.101.1.1 — Ribonuclease PH domain 2-like

结构域编号 domain_id	d2ba1g1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.4 — Ribonuclease PH domain 1-like

结构域编号 domain_id	d2ba1g2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.101 — Ribonuclease PH domain 2-like
超家族 Superfamily superfamily	d.101.1 — Ribonuclease PH domain 2-like
家族 Family family	d.101.1.1 — Ribonuclease PH domain 2-like

结构域编号 domain_id	d2ba1h1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.4 — Ribonuclease PH domain 1-like

结构域编号 domain_id	d2ba1h2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.101 — Ribonuclease PH domain 2-like
超家族 Superfamily superfamily	d.101.1 — Ribonuclease PH domain 2-like
家族 Family family	d.101.1.1 — Ribonuclease PH domain 2-like

结构域编号 domain_id	d2ba1i1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.4 — Ribonuclease PH domain 1-like

结构域编号 domain_id	d2ba1i2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.101 — Ribonuclease PH domain 2-like
超家族 Superfamily superfamily	d.101.1 — Ribonuclease PH domain 2-like
家族 Family family	d.101.1.1 — Ribonuclease PH domain 2-like

CATH v4.4 (15 domains)

结构域编号 domain_id	2ba1A01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily	100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain

结构域编号 domain_id	2ba1A02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily	140 — Nucleic acid-binding proteins

结构域编号 domain_id	2ba1A03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	70 — Ubiquitin Ligase Nedd4; Chain: W;
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	2ba1B01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily	100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain

结构域编号 domain_id	2ba1B02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily	140 — Nucleic acid-binding proteins

结构域编号 domain_id	2ba1B03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	70 — Ubiquitin Ligase Nedd4; Chain: W;
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	2ba1C01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily	100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain

结构域编号 domain_id	2ba1C02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	40 — Beta Barrel
拓扑 Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily	140 — Nucleic acid-binding proteins

结构域编号 domain_id	2ba1C03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	20 — Single Sheet
拓扑 Topology topology	70 — Ubiquitin Ligase Nedd4; Chain: W;
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	2ba1D01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	70 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	2ba1E01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	70 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	2ba1F01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	70 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	2ba1G01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	70 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	2ba1H01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	70 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	2ba1I01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	70 — GHMP Kinase, N-terminal domain

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7. 引用文献 (1)

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晶胞边长 a length_a	137.460 Å
晶胞边长 b length_b	137.460 Å
晶胞边长 c length_c	261.010 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	24

空间群 space_group	P 43 2 2
空间群编号 int_tables_number	95
晶系 crystal_system	—

分辨率 resolution	2.700 Å
最低分辨率 resolution_low	20.000 Å
R 因子 r_factor	—
R_work r_work	0.2240
R_free r_free	0.2750

fit	/app/data/saxs_computation/ba/2ba1/2ba1.fit
pddf	/app/data/pr_computation/ba/2ba1/2ba1.pr.out
profile	/app/data/saxs_computation/ba/2ba1/2ba1.dat

Archaeal exosome core

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

5. 实体与聚合体信息 (5)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 polymer

聚合体 Polymer

来源 Source

Entity 4 non-polymer

Entity 5 water

6. 折叠分类 (SCOP + CATH) 27 domains

SCOP 2.08 (12 domains)

CATH v4.4 (15 domains)

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)