SAXSdb — 2bf5

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1. Protein Identity and Related Structures Protein Identity & Related Structures

TOLUENE-4-MONOOXYGENASE SYSTEM PROTEIN D

PSEUDOMONAS MENDOCINA

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein monomer	Monomer	Protein 1	water × 1	Consistent with protein count
2	Protein monomer	Monomer	Protein 1	water × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TMOD_PSEME
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–98； UniProt 5–102 Author chain B； PDBConstruct 1–98； UniProt 5–102

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2bf5
Deposition date deposition_date	2004-12-03
Structure title title	Crystal structure of a toluene 4-monooxygenase catalytic effector protein variant missing four N-terminal residues (delta-N4 T4moD)
Keywords keywords	;CATALYTIC EFFECTOR PROTEIN, N-TERMINAL TRUNCATED MUTANT, AROMATIC HYDROCARBON CATABOLISM, OXIDOREDUCTASE, MONOOXYGENASE, TOLUENE OXIDATION, MOLECULAR REPLACEMENT ;; OXIDOREDUCTASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2bf5__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2bf5__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2bf5__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	14.10 Å
Rg (electron density)	12.55 Å
Total Rg	13.89 Å
Atom count	734
Residues	92
Excluded volume	13001 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2bf5__assembly_1__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`2bf5__assembly_2__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

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6. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_id	d2bf5a_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.137 — Monooxygenase (hydroxylase) regulatory protein
Superfamily Superfamily superfamily	d.137.1 — Monooxygenase (hydroxylase) regulatory protein
Family Family family	d.137.1.1 — Monooxygenase (hydroxylase) regulatory protein

Domain ID domain_id	d2bf5b_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.137 — Monooxygenase (hydroxylase) regulatory protein
Superfamily Superfamily superfamily	d.137.1 — Monooxygenase (hydroxylase) regulatory protein
Family Family family	d.137.1.1 — Monooxygenase (hydroxylase) regulatory protein

CATH v4.4 (2 domains)

Domain ID domain_id	2bf5A00
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	56 — Phenol Hydroxylase P2 Protein
Homologous superfamily homologous superfamily	10 — Monooxygenase component MmoB/DmpM

Domain ID domain_id	2bf5B00
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	56 — Phenol Hydroxylase P2 Protein
Homologous superfamily homologous superfamily	10 — Monooxygenase component MmoB/DmpM

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7. Citations (6)

Crystal Structures and Functional Studies of T4Mod, the Toluene 4-Monooxygenase Catalytic Effector Protein

Biochemistry (2005)

PMID: 15882052 · DOI: 10.1021/BI047459G PRIMARY

;Crystallization and Preliminary Analysis of Native and N-Terminal Truncated Isoforms of Toluene-4- Monooxygenase Catalytic Effector Protein ;

Acta Crystallogr.,Sect.D (2003)

PMID: 12595730 · DOI: 10.1107/S0907444903000416

;Combined Participation of Hydroxylase Active Site Residues and Effector Protein Binding in a Para to Ortho Modulation of Toluene 4-Monooxygenase Regiospecificity ;

Biochemistry (2002)

PMID: 11863457 · DOI: 10.1021/BI012036P

Solution Structure of the Toluene 4-Monooxygenase Effector Protein (T4Mod)

Biochemistry (2001)

PMID: 11297417 · DOI: 10.1021/BI0013703

Application of Fed-Batch Fermentation to the Preparation of Isotopically Labeled or Selenomethionyl-Labeled Proteins

Protein Expr.Purif. (1999)

PMID: 10336868 · DOI: 10.1006/PREP.1999.1067

;Recombinant Toluene 4-Monooxygenase: Catalytic and Mossbauer Studies of the Purified Diiron and Rieske Components of a Four Protein Complex ;

Biochemistry (1996)

PMID: 8703915 · DOI: 10.1021/BI960456M

Unit-cell length a length_a	86.215 Å
Unit-cell length b length_b	86.215 Å
Unit-cell length c length_c	86.215 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	24

Space group space_group	P 21 3
Space-group number int_tables_number	198
Crystal system crystal_system	—

Resolution resolution	1.710 Å
Low-resolution limit resolution_low	37.000 Å
R factor r_factor	0.1570
R_work r_work	0.1540
R_free r_free	0.1850

Description description	TOLUENE-4-MONOOXYGENASE SYSTEM PROTEIN D
Molecular weight formula_weight	11125.4 kDa
EC 编号 ec_number	1.14.13.-
Source method src_method	man
Copy count entity_count	2

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	1706

Crystal structure of a toluene 4-monooxygenase catalytic effector protein variant missing four N-terminal residues (delta-N4 T4moD)

1. Protein Identity and Related Structures Protein Identity & Related Structures

TOLUENE-4-MONOOXYGENASE SYSTEM PROTEIN D

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2bf5__assembly_1__model_1

2bf5__assembly_1__model_1 | I(q)

2bf5__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

CATH v4.4 (2 domains)

7. Citations (6)

Source organism organism	PSEUDOMONAS MENDOCINA
NCBI taxonomy ID ncbi_taxonomy_id	300
Host organism host_organism	ESCHERICHIA COLI

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	238

Crystal structure of a toluene 4-monooxygenase catalytic effector protein variant missing four N-terminal residues (delta-N4 T4moD)

1. Protein Identity and Related Structures Protein Identity & Related Structures

TOLUENE-4-MONOOXYGENASE SYSTEM PROTEIN D

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2bf5__assembly_1__model_1

2bf5__assembly_1__model_1 | I(q)

2bf5__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

CATH v4.4 (2 domains)

7. Citations (6)