2bh1

;X-ray structure of the general secretion pathway complex of the N- terminal domain of EpsE and the cytosolic domain of EpsL of Vibrio cholerae ;

Method: X-RAY DIFFRACTION Dmax: 107.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 2bh1

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2bh1
下载 Download

1. 结构基本信息 1. Structure Basics

条目编号 entry_id2bh1
沉积日期 deposition_date2005-01-06
结构标题 title;X-ray structure of the general secretion pathway complex of the N- terminal domain of EpsE and the cytosolic domain of EpsL of Vibrio cholerae ;
关键词 keywordsTRANSPORT PROTEIN, TYPE II SECRETION, VIBRIO CHOLERAE, EPS, GSP, TRANSMEMBRANE, TRANSPORT, ATP-BINDING; TRANSPORT PROTEIN
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier31.80
回转半径 Rg (电子) rg_electron31.28
零角强度 I(0) i072013300.00
分子量 molecular_weight68971.0 kDa
排除体积 excluded_volume87188 ų
包络体积 envelope_volume114230 ų
水化壳体积 shell_volume31710 ų
包络直径 envelope_diameter111.9
壳层 Rg shell_rg36.35
包络 Rg envelope_rg31.42
形状 Rg shape_rg31.29
总 Rg total_rg31.72
总原子数 total_atoms4869
残基数 n_residues612
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax107.8
Rg (实空间) rg_real32.04
Rg 误差 (实空间) rg_real_error0.96
I(0) (实空间) i0_real7.2010e+07
I(0) 误差 (实空间) i0_real_error1.1650e+06
Rg (倒空间) rg_reciprocal31.94
I(0) (倒空间) i0_reciprocal72010000.0000
解质量估计 total_estimate0.8517
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary32.3
偏度 Skewness skewness0.499
峰度 Kurtosis kurtosis-0.369
角度范围 angular_range— – 0.2500 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha16620000.0000
实空间数据点数 n_real_points51
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.774; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.901; Smooth: 0.845

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (4)

6. 折叠分类 (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

结构域编号 domain_idd2bh1a1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.1 — Actin-like ATPase domain
家族 Family familyc.55.1.0 — automated matches
结构域编号 domain_idd2bh1a2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.1 — Actin-like ATPase domain
家族 Family familyc.55.1.11 — Cyto-EpsL domain
结构域编号 domain_idd2bh1b1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.1 — Actin-like ATPase domain
家族 Family familyc.55.1.0 — automated matches
结构域编号 domain_idd2bh1b2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.1 — Actin-like ATPase domain
家族 Family familyc.55.1.11 — Cyto-EpsL domain
结构域编号 domain_idd2bh1x1
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.52 — Alpha-lytic protease prodomain-like
超家族 Superfamily superfamilyd.52.10 — EspE N-terminal domain-like
家族 Family familyd.52.10.1 — GSPII protein E N-terminal domain-like
结构域编号 domain_idd2bh1y_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.52 — Alpha-lytic protease prodomain-like
超家族 Superfamily superfamilyd.52.10 — EspE N-terminal domain-like
家族 Family familyd.52.10.1 — GSPII protein E N-terminal domain-like

CATH v4.4 (6 domains)

结构域编号 domain_id2bh1A01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily380
结构域编号 domain_id2bh1A02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily370 — GspL cytoplasmic domain, C-terminal subdomain
结构域编号 domain_id2bh1B01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily380
结构域编号 domain_id2bh1B02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily370 — GspL cytoplasmic domain, C-terminal subdomain
结构域编号 domain_id2bh1X00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology300 — GMP Synthetase; Chain A, domain 3
同源超家族 H-superfamily homologous superfamily160 — Type II secretion system, protein E, N-terminal domain
结构域编号 domain_id2bh1Y00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology300 — GMP Synthetase; Chain A, domain 3
同源超家族 H-superfamily homologous superfamily160 — Type II secretion system, protein E, N-terminal domain

7. 引用文献 (1)

8. 文件与曲线 (10)