2bt2

Structure of the regulator of G-protein signaling 16

Method: X-RAY DIFFRACTION D_max: 114.3 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	2bt2
沉积日期 deposition_date	2005-05-25
结构标题 title	Structure of the regulator of G-protein signaling 16
关键词 keywords	GTPASE ACTIVATING PROTEIN, SIGNAL TRANSDUCTION INHIBITOR, SIGNALING PROTEIN; SIGNALING PROTEIN
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	34.12 Å
回转半径 Rg (电子) rg_electron	34.00 Å
零角强度 I(0) i0	99357300.00
分子量 molecular_weight	78441.0 kDa
排除体积 excluded_volume	97712 Å³
包络体积 envelope_volume	140730 Å³
水化壳体积 shell_volume	36239 Å³
包络直径 envelope_diameter	119.1 Å
壳层 Rg shell_rg	38.60 Å
包络 Rg envelope_rg	33.77 Å
形状 Rg shape_rg	34.04 Å
总 Rg total_rg	34.26 Å
总原子数 total_atoms	5536
残基数 n_residues	711
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	114.3 Å
Rg (实空间) rg_real	34.15 Å
Rg 误差 (实空间) rg_real_error	1.26 Å
I(0) (实空间) i0_real	9.9360e+07
I(0) 误差 (实空间) i0_real_error	1.7510e+06
Rg (倒空间) rg_reciprocal	34.13 Å
I(0) (倒空间) i0_reciprocal	99360000.0000
解质量估计 total_estimate	0.8867
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	37.3 Å
偏度 Skewness skewness	0.312
峰度 Kurtosis kurtosis	-0.440
角度范围 angular_range	— – 0.2300 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	4667000.0000
实空间数据点数 n_real_points	47
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.857; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.958

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (2)

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6. 折叠分类 (SCOP + CATH) 20 domains

SCOP 2.08 (10 domains)

结构域编号 domain_id	d2bt2a1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.91 — Regulator of G-protein signaling, RGS
超家族 Superfamily superfamily	a.91.1 — Regulator of G-protein signaling, RGS
家族 Family family	a.91.1.0 — automated matches

结构域编号 domain_id	d2bt2a2
类 Class class	l — Artifacts
折叠类型 Fold fold	l.1 — Tags
超家族 Superfamily superfamily	l.1.1 — Tags
家族 Family family	l.1.1.1 — Tags

结构域编号 domain_id	d2bt2b1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.91 — Regulator of G-protein signaling, RGS
超家族 Superfamily superfamily	a.91.1 — Regulator of G-protein signaling, RGS
家族 Family family	a.91.1.0 — automated matches

结构域编号 domain_id	d2bt2b2
类 Class class	l — Artifacts
折叠类型 Fold fold	l.1 — Tags
超家族 Superfamily superfamily	l.1.1 — Tags
家族 Family family	l.1.1.1 — Tags

结构域编号 domain_id	d2bt2c1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.91 — Regulator of G-protein signaling, RGS
超家族 Superfamily superfamily	a.91.1 — Regulator of G-protein signaling, RGS
家族 Family family	a.91.1.0 — automated matches

结构域编号 domain_id	d2bt2c2
类 Class class	l — Artifacts
折叠类型 Fold fold	l.1 — Tags
超家族 Superfamily superfamily	l.1.1 — Tags
家族 Family family	l.1.1.1 — Tags

结构域编号 domain_id	d2bt2d1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.91 — Regulator of G-protein signaling, RGS
超家族 Superfamily superfamily	a.91.1 — Regulator of G-protein signaling, RGS
家族 Family family	a.91.1.0 — automated matches

结构域编号 domain_id	d2bt2d2
类 Class class	l — Artifacts
折叠类型 Fold fold	l.1 — Tags
超家族 Superfamily superfamily	l.1.1 — Tags
家族 Family family	l.1.1.1 — Tags

结构域编号 domain_id	d2bt2e1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.91 — Regulator of G-protein signaling, RGS
超家族 Superfamily superfamily	a.91.1 — Regulator of G-protein signaling, RGS
家族 Family family	a.91.1.0 — automated matches

结构域编号 domain_id	d2bt2e2
类 Class class	l — Artifacts
折叠类型 Fold fold	l.1 — Tags
超家族 Superfamily superfamily	l.1.1 — Tags
家族 Family family	l.1.1.1 — Tags

CATH v4.4 (10 domains)

结构域编号 domain_id	2bt2A01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	196 — Regulator of G-protein Signalling 4; domain 1
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	2bt2A02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	167 — Regulator of G-protein Signalling 4; domain 2
同源超家族 H-superfamily homologous superfamily	10 — Regulator of G-protein Signalling 4, domain 2

结构域编号 domain_id	2bt2B01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	196 — Regulator of G-protein Signalling 4; domain 1
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	2bt2B02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	167 — Regulator of G-protein Signalling 4; domain 2
同源超家族 H-superfamily homologous superfamily	10 — Regulator of G-protein Signalling 4, domain 2

结构域编号 domain_id	2bt2C01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	196 — Regulator of G-protein Signalling 4; domain 1
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	2bt2C02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	167 — Regulator of G-protein Signalling 4; domain 2
同源超家族 H-superfamily homologous superfamily	10 — Regulator of G-protein Signalling 4, domain 2

结构域编号 domain_id	2bt2D01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	196 — Regulator of G-protein Signalling 4; domain 1
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	2bt2D02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	167 — Regulator of G-protein Signalling 4; domain 2
同源超家族 H-superfamily homologous superfamily	10 — Regulator of G-protein Signalling 4, domain 2

结构域编号 domain_id	2bt2E01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	196 — Regulator of G-protein Signalling 4; domain 1
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	2bt2E02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	167 — Regulator of G-protein Signalling 4; domain 2
同源超家族 H-superfamily homologous superfamily	10 — Regulator of G-protein Signalling 4, domain 2

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7. 引用文献 (1)

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晶胞边长 a length_a	160.585 Å
晶胞边长 b length_b	31.743 Å
晶胞边长 c length_c	151.766 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	94.39 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	20

空间群 space_group	C 1 2 1
空间群编号 int_tables_number	5
晶系 crystal_system	—

分辨率 resolution	1.900 Å
最低分辨率 resolution_low	30.000 Å
R 因子 r_factor	0.1950
R_work r_work	0.1920
R_free r_free	0.2500

描述 description	REGULATOR OF G-PROTEIN SIGNALING 16
分子量 formula_weight	18389.6 kDa
来源方法 src_method	man
拷贝数 entity_count	5

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E
原子数 atom_count	5953

Structure of the regulator of G-protein signaling 16

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 water

6. 折叠分类 (SCOP + CATH) 20 domains

SCOP 2.08 (10 domains)

CATH v4.4 (10 domains)

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)

来源生物 organism	HOMO SAPIENS
通用名 common_name	HUMAN
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	ESCHERICHIA COLI

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	376

fit	/app/data/saxs_computation/bt/2bt2/2bt2.fit
pddf	/app/data/pr_computation/bt/2bt2/2bt2.pr.out
profile	/app/data/saxs_computation/bt/2bt2/2bt2.dat