2c3u

Crystal Structure Of Pyruvate-Ferredoxin Oxidoreductase From Desulfovibrio africanus, Oxygen inhibited form

Method: X-RAY DIFFRACTION Dmax: 124.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 2c3u

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2c3u
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2c3u
沉积日期 deposition_date2005-10-12
结构标题 titleCrystal Structure Of Pyruvate-Ferredoxin Oxidoreductase From Desulfovibrio africanus, Oxygen inhibited form
关键词 keywords;OXIDOREDUCTASE, 4FE-4S, IRON, IRON-SULFUR, IRON-SULFUR CLUSTER, PYRUVATE CATABOLISM, TPP-DEPENDENT ENZYME, METAL-BINDING, ELECTRON TRANSPORT ;; OXIDOREDUCTASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier39.07
回转半径 Rg (电子) rg_electron38.65
零角强度 I(0) i01107130000.00
分子量 molecular_weight270380.0 kDa
排除体积 excluded_volume336520 ų
包络体积 envelope_volume404470 ų
水化壳体积 shell_volume81174 ų
包络直径 envelope_diameter134.7
壳层 Rg shell_rg47.96
包络 Rg envelope_rg39.26
形状 Rg shape_rg38.64
总 Rg total_rg39.15
总原子数 total_atoms18884
残基数 n_residues2462
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax124.2
Rg (实空间) rg_real38.90
Rg 误差 (实空间) rg_real_error0.71
I(0) (实空间) i0_real1.1070e+09
I(0) 误差 (实空间) i0_real_error1.6740e+07
Rg (倒空间) rg_reciprocal39.01
I(0) (倒空间) i0_reciprocal1107000000.0000
解质量估计 total_estimate0.8751
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary46.6
偏度 Skewness skewness0.295
峰度 Kurtosis kurtosis-0.246
角度范围 angular_range— – 0.2000 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha299100000.0000
实空间数据点数 n_real_points41
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.874; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.984; Smooth: 0.767

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (7)

6. 折叠分类 (SCOP + CATH) 24 domains

SCOP 2.08 (10 domains)

结构域编号 domain_idd2c3ua1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.8 — PFOR Pyr module
结构域编号 domain_idd2c3ua2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.12 — PFOR PP module
结构域编号 domain_idd2c3ua3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.48 — TK C-terminal domain-like
超家族 Superfamily superfamilyc.48.1 — TK C-terminal domain-like
家族 Family familyc.48.1.3 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain II
结构域编号 domain_idd2c3ua4
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.64 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain III
超家族 Superfamily superfamilyc.64.1 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain III
家族 Family familyc.64.1.1 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain III
结构域编号 domain_idd2c3ua5
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
家族 Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins
结构域编号 domain_idd2c3ub1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.8 — PFOR Pyr module
结构域编号 domain_idd2c3ub2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.36 — Thiamin diphosphate-binding fold (THDP-binding)
超家族 Superfamily superfamilyc.36.1 — Thiamin diphosphate-binding fold (THDP-binding)
家族 Family familyc.36.1.12 — PFOR PP module
结构域编号 domain_idd2c3ub3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.48 — TK C-terminal domain-like
超家族 Superfamily superfamilyc.48.1 — TK C-terminal domain-like
家族 Family familyc.48.1.3 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain II
结构域编号 domain_idd2c3ub4
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.64 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain III
超家族 Superfamily superfamilyc.64.1 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain III
家族 Family familyc.64.1.1 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain III
结构域编号 domain_idd2c3ub5
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
家族 Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins

CATH v4.4 (14 domains)

结构域编号 domain_id2c3uA01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id2c3uA02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily920
结构域编号 domain_id2c3uA03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology920 — Pyruvate-ferredoxin Oxidoreductase; domain 3
同源超家族 H-superfamily homologous superfamily10 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain III
结构域编号 domain_id2c3uA04
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology780 — Pyruvate-ferredoxin Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Pyruvate-flavodoxin oxidoreductase, EKR domain
结构域编号 domain_id2c3uA05
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily20
结构域编号 domain_id2c3uA06
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id2c3uA07
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology790 — Pyruvate-ferredoxin Oxidoreductase; domain 7
同源超家族 H-superfamily homologous superfamily10 — Pyruvate-ferredoxin Oxidoreductase; domain 7
结构域编号 domain_id2c3uB01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id2c3uB02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily920
结构域编号 domain_id2c3uB03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology920 — Pyruvate-ferredoxin Oxidoreductase; domain 3
同源超家族 H-superfamily homologous superfamily10 — Pyruvate-ferredoxin oxidoreductase, PFOR, domain III
结构域编号 domain_id2c3uB04
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology780 — Pyruvate-ferredoxin Oxidoreductase; domain 4
同源超家族 H-superfamily homologous superfamily10 — Pyruvate-flavodoxin oxidoreductase, EKR domain
结构域编号 domain_id2c3uB05
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily20
结构域编号 domain_id2c3uB06
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily970 — Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains
结构域编号 domain_id2c3uB07
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology790 — Pyruvate-ferredoxin Oxidoreductase; domain 7
同源超家族 H-superfamily homologous superfamily10 — Pyruvate-ferredoxin Oxidoreductase; domain 7

7. 引用文献 (2)

8. 文件与曲线 (10)