2c7e

REVISED ATOMIC STRUCTURE FITTING INTO A GROEL(D398A)-ATP7 CRYO-EM MAP (EMD 1047)

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

60 KDA CHAPERONIN

ESCHERICHIA COLI

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 14	POTASSIUM ION × 7 MAGNESIUM ION × 7 ADENOSINE-5'-TRIPHOSPHATE × 7 water × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CH60_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–547； UniProt 1–547 Author chain B； PDBConstruct 1–547； UniProt 1–547 Author chain C； PDBConstruct 1–547； UniProt 1–547 Author chain D； PDBConstruct 1–547； UniProt 1–547 Author chain E； PDBConstruct 1–547； UniProt 1–547 Author chain F； PDBConstruct 1–547； UniProt 1–547 Author chain G； PDBConstruct 1–547； UniProt 1–547 Author chain H； PDBConstruct 1–547； UniProt 1–547 Author chain I； PDBConstruct 1–547； UniProt 1–547 Author chain J； PDBConstruct 1–547； UniProt 1–547 Author chain K； PDBConstruct 1–547； UniProt 1–547 Author chain L； PDBConstruct 1–547； UniProt 1–547 Author chain M； PDBConstruct 1–547； UniProt 1–547 Author chain N； PDBConstruct 1–547； UniProt 1–547

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2c7e
Deposition date deposition_date	2005-11-22
Structure title title	REVISED ATOMIC STRUCTURE FITTING INTO A GROEL(D398A)-ATP7 CRYO-EM MAP (EMD 1047)
Keywords keywords	CELL CYCLE, ATP-BINDING, CHAPERONE, CHAPERONIN, D398A, HP60 CLASS, CELL DIVISION, NUCLEOTIDE-BINDING, PHOSPHORYLATION; CELL CYCLE
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2c7e__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2c7e__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2c7e__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	67.00 Å
Rg (electron density)	66.32 Å
Total Rg	66.36 Å
Atom count	54201
Residues	7350
Excluded volume	971040 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2c7e__assembly_1__model_1`	tetradecameric （14）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	60 KDA CHAPERONIN
Molecular weight formula_weight	57188.4 kDa
Mutation mutation	YES
Source method src_method	man
Copy count entity_count	14

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H,I,J,K,L,M,N
Atom count atom_count	54243

AAKDVKFGNDAGVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTVAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM

Source Source

Source organism organism	ESCHERICHIA COLI
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 non-polymer

Description description	POTASSIUM ION
Molecular weight formula_weight	39.1 kDa
Source method src_method	syn
Copy count entity_count	7

Entity 3 Entity 3 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	7

Entity 4 Entity 4 non-polymer

Description description	ADENOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	507.2 kDa
Source method src_method	syn
Copy count entity_count	7

Entity 5 Entity 5 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	42

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6. Fold Classification (SCOP + CATH) 14 domains

SCOP 2.08 (14 domains)

Domain ID domain_id	d2c7ea_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7eb_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7ec_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7ed_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7ee_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7ef_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7eg_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7eh_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7ei_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7ej_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7ek_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7el_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7em_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

Domain ID domain_id	d2c7en_
Class class	i — Low resolution protein structures
Fold Fold fold	i.16 — GroE chaperon
Superfamily Superfamily superfamily	i.16.1 — GroE chaperon
Family Family family	i.16.1.1 — GroE chaperon

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	14.900 Å
Low-resolution limit resolution_low	— Å
R factor r_factor	—
R_work r_work	—
R_free r_free	—

Resolution resolution	14.90 Å
Resolution测定Method resolution_method	PROJECTION MATCHING-BASED ANGULAR REFINEMENT AND ITERATIVE ALGEBRAIC RECONSTRUCTION IN SPIDER
颗粒数 num_particles	6404

REVISED ATOMIC STRUCTURE FITTING INTO A GROEL(D398A)-ATP7 CRYO-EM MAP (EMD 1047)

1. Protein Identity and Related Structures Protein Identity & Related Structures

60 KDA CHAPERONIN

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2c7e__assembly_1__model_1

2c7e__assembly_1__model_1 | I(q)

2c7e__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 14 domains

SCOP 2.08 (14 domains)

7. Citations (1)

REVISED ATOMIC STRUCTURE FITTING INTO A GROEL(D398A)-ATP7 CRYO-EM MAP (EMD 1047)

1. Protein Identity and Related Structures Protein Identity & Related Structures

60 KDA CHAPERONIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2c7e__assembly_1__model_1

2c7e__assembly_1__model_1 | I(q)

2c7e__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 14 domains

SCOP 2.08 (14 domains)

7. Citations (1)