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2csm

TYR-BOUND T-STATE OF YEAST CHORISMATE MUTASE

Method: X-RAY DIFFRACTION Dmax: 65.3 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

CHORISMATE MUTASE

OrganismNot specified

UniProt P32178

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 1–256 Not recorded TYR TYROSINE × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.80 Å R-free 0.321

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2CSM Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1CSM THE CRYSTAL STRUCTURE OF ALLOSTERIC CHORISMATE MUTASE AT 2.2 ANGSTROMS RESOLUTION Deposited 1994-08-22 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–256（256 aa）
Chain B 1–256（256 aa）
Not recorded TRP TRYPTOPHAN × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.20 Å R-free 0.310
3CSM STRUCTURE OF YEAST CHORISMATE MUTASE WITH BOUND TRP AND AN ENDOOXABICYCLIC INHIBITOR Deposited 1997-07-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–256（256 aa）
Chain B 1–256（256 aa）
Not recorded TRP TRYPTOPHAN × 2 TSA 8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；HANGING DROP, 19 % (W/V) PEG MONOMETHYLETHER 5000, 5 MM DTT, 0.15 M TRIS PH 8.0, 0.15 M SODIUM ACETATE, 16 MM TRYPTOPHAN, 3 MM INHIBITOR, 10 MG/ML PROTEIN, vapor diffusion - hanging drop
Resolution 3.00 Å R-free 0.247
3CSM STRUCTURE OF YEAST CHORISMATE MUTASE WITH BOUND TRP AND AN ENDOOXABICYCLIC INHIBITOR Deposited 1997-07-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–256（256 aa）
Not recorded TRP TRYPTOPHAN × 2 TSA 8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；HANGING DROP, 19 % (W/V) PEG MONOMETHYLETHER 5000, 5 MM DTT, 0.15 M TRIS PH 8.0, 0.15 M SODIUM ACETATE, 16 MM TRYPTOPHAN, 3 MM INHIBITOR, 10 MG/ML PROTEIN, vapor diffusion - hanging drop
Resolution 3.00 Å R-free 0.247
3CSM STRUCTURE OF YEAST CHORISMATE MUTASE WITH BOUND TRP AND AN ENDOOXABICYCLIC INHIBITOR Deposited 1997-07-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 1–256（256 aa）
Not recorded TRP TRYPTOPHAN × 2 TSA 8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；HANGING DROP, 19 % (W/V) PEG MONOMETHYLETHER 5000, 5 MM DTT, 0.15 M TRIS PH 8.0, 0.15 M SODIUM ACETATE, 16 MM TRYPTOPHAN, 3 MM INHIBITOR, 10 MG/ML PROTEIN, vapor diffusion - hanging drop
Resolution 3.00 Å R-free 0.247
4CSM YEAST CHORISMATE MUTASE + TYR + ENDOOXABICYCLIC INHIBITOR Deposited 1997-07-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–256（256 aa）
Chain B 1–256（256 aa）
Not recorded TYR TYROSINE × 2 TSA 8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；HANGING DROP, 32 % (W/V) PEG MONOMETHYLETHER 500, 4 MM DTT, 0.1 M TRIS PH 9.0, 0.1 M SODIUM CHLORIDE, 2 MM TYROSINE, 3 MM INHIBITOR, 10 MG/ML PROTEIN, vapor diffusion - hanging drop
Resolution 2.80 Å R-free 0.270
4CSM YEAST CHORISMATE MUTASE + TYR + ENDOOXABICYCLIC INHIBITOR Deposited 1997-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 1–256（256 aa）
Not recorded TYR TYROSINE × 2 TSA 8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；HANGING DROP, 32 % (W/V) PEG MONOMETHYLETHER 500, 4 MM DTT, 0.1 M TRIS PH 9.0, 0.1 M SODIUM CHLORIDE, 2 MM TYROSINE, 3 MM INHIBITOR, 10 MG/ML PROTEIN, vapor diffusion - hanging drop
Resolution 2.80 Å R-free 0.270
4CSM YEAST CHORISMATE MUTASE + TYR + ENDOOXABICYCLIC INHIBITOR Deposited 1997-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–256（256 aa）
Not recorded TYR TYROSINE × 2 TSA 8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；HANGING DROP, 32 % (W/V) PEG MONOMETHYLETHER 500, 4 MM DTT, 0.1 M TRIS PH 9.0, 0.1 M SODIUM CHLORIDE, 2 MM TYROSINE, 3 MM INHIBITOR, 10 MG/ML PROTEIN, vapor diffusion - hanging drop
Resolution 2.80 Å R-free 0.270
5CSM YEAST CHORISMATE MUTASE, T226S MUTANT, COMPLEX WITH TRP Deposited 1997-07-14 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–256（256 aa）
Mutation：T226S TRP TRYPTOPHAN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；HANGING DROP, 19 % PEG 3350, 3 MM DTT, 0.16 M SODIUM ACETATE PH 5.0, 16 MM TRYPTOPHAN, 10 MG/ML PROTEIN, vapor diffusion - hanging drop
Resolution 2.00 Å R-free 0.236

4 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CHMU_YEAST
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–256； UniProt 1–256

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2csm

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2csm
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2csm
Deposition date deposition_date1995-11-24
Structure title titleTYR-BOUND T-STATE OF YEAST CHORISMATE MUTASE
Keywords keywordsALLOSTERIC PROTEIN, COMPLEX (ISOMERASE-PEPTIDE), COMPLEX (ISOMERASE-PEPTIDE) complex; COMPLEX (ISOMERASE/PEPTIDE)
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier20.24 Å
Radius of gyration Rg (electron density) rg_electron19.20 Å
Forward intensity I(0) i013201100.00
Molecular weight molecular_weight28668.0 kDa
Excluded volume excluded_volume36554 ų
Envelope volume envelope_volume42803 ų
Hydration-shell volume shell_volume18895 ų
Envelope diameter envelope_diameter68.6 Å
Shell Rg shell_rg25.21 Å
Envelope Rg envelope_rg19.38 Å
Shape Rg shape_rg19.19 Å
Total Rg total_rg20.16 Å
Total atoms total_atoms2025
Residues n_residues245
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax65.3 Å
Rg (real space) rg_real20.16 Å
Rg uncertainty (real space) rg_real_error0.38 Å
I(0) (real space) i0_real1.3200e+07
I(0) uncertainty (real space) i0_real_error1.5430e+05
Rg (reciprocal space) rg_reciprocal20.18 Å
I(0) (reciprocal space) i0_reciprocal13200000.0000
Solution quality estimate total_estimate0.8171
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks3
Primary peak position r_peak_primary25.2 Å
Skewness Skewness skewness0.207
Kurtosis Kurtosis kurtosis-0.454
Angular range angular_range— – 0.3950 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha2730000.0000
Real-space data points n_real_points71
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.875; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd2csma_
Class classa — All alpha proteins
Fold Fold folda.130 — Chorismate mutase II
Superfamily Superfamily superfamilya.130.1 — Chorismate mutase II
Family Family familya.130.1.2 — Allosteric chorismate mutase

CATH v4.4 (1 domains)

Domain ID domain_id2csmA00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology590 — Chorismate Mutase, subunit A
Homologous superfamily homologous superfamily10 — Chorismate mutase, AroQ class superfamily, eukaryotic
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8. Citations (3)

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9. Files and Curves (10)