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2d4x

Crystal structure of a 26K fragment of HAP3 (FlgL)

Method: X-RAY DIFFRACTION Dmax: 58.0 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Flagellar hook-associated protein 3

Salmonella typhimurium

UniProt P16326

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 40–286 Fragment：FL26 No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION；pH 4.2；277 K；10-20% PEG 8000, 0.2M magnesium chloride, 0.1M Phosphate-citrate, pH 4.2, VAPOR DIFFUSION, temperature 277K Resolution 1.90 Å R-free 0.258

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2D4X Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9M68 the flagellar filament cap FliD in complex with FlgL Deposited 2025-03-07 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain C 51–273（223 aa）
Chain E 51–273（223 aa）
Chain F 51–273（223 aa）
Chain H 51–273（223 aa）
Chain J 51–273（223 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9U4R Structure of the intial complex in filament assembly at 3.23 angstroms resolution, conformation 2. Deposited 2025-03-20 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 73 PDB declaration: 73-meric
Chain BA 1–317（317 aa）
Chain BB 1–317（317 aa）
Chain BC 1–317（317 aa）
Chain BD 1–317（317 aa）
Chain BE 1–317（317 aa）
Chain BG 1–317（317 aa）
Chain BH 1–317（317 aa）
Chain BI 1–317（317 aa）
Chain BJ 1–317（317 aa）
Chain BK 1–317（317 aa）
Chain BL 1–317（317 aa）
Chain BM 1–317（317 aa）
Chain BN 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.23 Å
9U7Y Structure of the tip region of the intial complex in bacterial flagellar filament assembly at 3.68 angstroms resolution, conformation 3. Deposited 2025-03-25 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 60 PDB declaration: 60-meric
Chain BA 1–317（317 aa）
Chain BB 1–317（317 aa）
Chain BC 1–317（317 aa）
Chain BD 1–317（317 aa）
Chain BE 1–317（317 aa）
Chain BF 1–317（317 aa）
Chain BG 1–317（317 aa）
Chain BH 1–317（317 aa）
Chain BI 1–317（317 aa）
Chain BJ 1–317（317 aa）
Chain BK 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.68 Å

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name FLGL_SALTY
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–248； UniProt 40–286

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2d4x

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2d4x
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2d4x
Deposition date deposition_date2005-10-25
Structure title titleCrystal structure of a 26K fragment of HAP3 (FlgL)
Keywords keywordsMulti-domain protein, alpha-helical bundle, complex all-beta folds, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier21.27 Å
Radius of gyration Rg (electron density) rg_electron20.94 Å
Forward intensity I(0) i010187500.00
Molecular weight molecular_weight22660.0 kDa
Excluded volume excluded_volume27966 ų
Envelope volume envelope_volume33805 ų
Hydration-shell volume shell_volume15294 ų
Envelope diameter envelope_diameter87.9 Å
Shell Rg shell_rg25.11 Å
Envelope Rg envelope_rg21.87 Å
Shape Rg shape_rg20.92 Å
Total Rg total_rg21.62 Å
Total atoms total_atoms1592
Residues n_residues214
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax58.0 Å
Rg (real space) rg_real19.81 Å
Rg uncertainty (real space) rg_real_error0.12 Å
I(0) (real space) i0_real9.6900e+06
I(0) uncertainty (real space) i0_real_error1.0390e+05
Rg (reciprocal space) rg_reciprocal21.56 Å
I(0) (reciprocal space) i0_reciprocal10190000.0000
Solution quality estimate total_estimate0.6816
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary21.3 Å
Skewness Skewness skewness0.417
Kurtosis Kurtosis kurtosis-0.384
Angular range angular_range— – 0.3750 Å−1
Current regularization parameter α current_alpha3.0570
Highest regularization parameter α highest_alpha1332000.0000
Real-space data points n_real_points69
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.002; Oscil: 0.970; Stabil: 0.989; Sysdev: 0.000; Positv: 1.000; Valcen: 0.984; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id2d4xA00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1330 — f41 fragment of flagellin, N-terminal domain
Homologous superfamily homologous superfamily10 — f41 fragment of flagellin, N-terminal domain
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8. Citations (1)

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9. Files and Curves (10)