2ftc

Structural Model for the Large Subunit of the Mammalian Mitochondrial Ribosome

Method: ELECTRON MICROSCOPY D_max: 264.4 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	2ftc
沉积日期 deposition_date	2006-01-24
结构标题 title	Structural Model for the Large Subunit of the Mammalian Mitochondrial Ribosome
关键词 keywords	MITOCHONDRIAL RIBOSOME, LARGE RIBOSOMAL SUBUNIT, RIBOSOMAL RNA, RIBOSOME; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	75.55 Å
回转半径 Rg (电子) rg_electron	83.07 Å
零角强度 I(0) i0	2188350000.00
分子量 molecular_weight	279350.0 kDa
排除体积 excluded_volume	298670 Å³
包络体积 envelope_volume	919330 Å³
水化壳体积 shell_volume	117660 Å³
包络直径 envelope_diameter	259.2 Å
壳层 Rg shell_rg	63.04 Å
包络 Rg envelope_rg	67.78 Å
形状 Rg shape_rg	76.65 Å
总 Rg total_rg	82.99 Å
总原子数 total_atoms	1443
残基数 n_residues	1443
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	264.4 Å
Rg (实空间) rg_real	79.12 Å
Rg 误差 (实空间) rg_real_error	1.38 Å
I(0) (实空间) i0_real	2.1970e+09
I(0) 误差 (实空间) i0_real_error	4.4680e+07
Rg (倒空间) rg_reciprocal	75.18 Å
I(0) (倒空间) i0_reciprocal	2186000000.0000
解质量估计 total_estimate	0.8777
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	88.1 Å
偏度 Skewness skewness	0.552
峰度 Kurtosis kurtosis	0.029
角度范围 angular_range	— – 0.1050 Å⁻¹
当前正则化参数 α current_alpha	1.0710
最高正则化参数 α highest_alpha	385100000.0000
实空间数据点数 n_real_points	22
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.814; Stabil: 0.887; Sysdev: 1.000; Positv: 1.000; Valcen: 0.979; Smooth: 0.390

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (17)

Entity 1 polymer

描述 description	Mitochondrial 16S ribosomal RNA
分子量 formula_weight	504151.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	R
原子数 atom_count	3536

ACUAGACCUAGCCCAAAGAUACCCUCUCGACUAAACAACCAAGAUAGAAUAAAACAAAACAUUUAAUCCCAAUUUAAAGUAUAGGAGAUAGAAAUCUAAGUACGGCGCUAUAGAGAAAGUACCGCAAGGGAACGAUGAAAGAAAAAAACUAAAAGUAUAAAAAAGCAAAGAUUACCCCUUGUACCUUUUGCAUAAUGAAUUAACUAGUAUAAGACUUAACAAAAUGAAUUUUAGCUAAGCAGCCCGAAACCAGACGAGCUACUCACAAACAGUUUACCAAGAACUAACUCAUCUAUGUGGCAAAAUAGUGAGAAGAUUUGUAAGUAGAGGUGACAUGCCUAACGAGCCUGGUGAUAGCUGGUUGUCCAGAAAAUGAAUCUAAGUUCAGCUUUAAAGAUACCAAAAAUUCAAAUAAACCCCACUGUAGCUUUAAAAGUUAGUCUAAAAAGGUACAGCCUUUUAGAAACGGAUACAACCUUGACUAGAGAGUAAAAUUUAACACUACCAUAGUAGGCCUAAAAGCAGCCAUCAAUUAAGAAAGCGUUAAAGCUCAACAACAAAAAUUAAAUAGAUUCCAACAACAAAUGAUUAACUCCUAGCCCCAAUACUGGACUAAUCUAUUAUAGAAUAGAAGCAAUAAUGUUAAUAUGAGUAACAAGAAAAAUUUUCUCCUUGCAUAAGUCUAAGUCAGUGCCUGAUAAUACUCUGACCACUAACAGUCAAUAAAAAUAAUCCAACAAUAAACAAUUUAUUGAUUAUACUGUUAACCCAACACAGGAGUGCAUCUAAGGAAAGAUUAAAAGAAGUAAAAGGAACUCGGCAAACACAAACCCCGCCUGUUUACCAAAAACAUCACCUCCAGCAUUCCCAGUAUUGGAGGCAUUGCCUGCCCAGUGACAACUGUUUAACGGCCGCGGUAUCCUGACCGUGCAAAGGUAGCAUAAUCAUUUGUUCUCUAAAUAAGGACUUGUAUGAAUGGCCGCACGAGGGUUUUACUGUCUCUUACUUCCAAUCAGUGAAAUUGACCUUCCCGUGAAGAGGCGGGAAUGCACAAAUAAGACGAGAAGACCCUAUGGAGCUUUAACUAACCAACCCAAAGAGAAUAGAUUUAACCAUUAAGGAAUAACAACAAUCUCCAUGAGUUGGUAGUUUCGGUUGGGGUGACCUCGGAGAAUAAAAAAUCCUCCGAGCGAUUUUAAAGACUAGACCCACAAGUCAAAUCACUCUAUCGCUCAUUGAUCCAAAAACUUGAUCAACGGAACAAGUUACCCUAGGGAUAACAGCGCAAUCCUAUUCAAGAGUCCAUAUCGACAAUAGGGUUUACGACCUCGAUGUUGGAUCAGGACAUCCUGAUGGUGCAACCGCUAUCAAAGGUUCGUUUGUUCAACGAUUAAAGUCCUACGUGAUCUGAGUUCAGACCGGAGUAAUCCAGGUCGGUUUCUAUCUAUUACGUAUUUCUCCCAGUACGAAAGGACAAGAGAAAUAAGGCCAACUUUAAAUCAAGCGCCUUAAGACAACCAAUGAUAACAUCUCAACUGACAACACAAAACCCUGCCCUAGAACAGGGC

Entity 2 polymer

描述 description	Mitochondrial ribosomal protein L1
分子量 formula_weight	21224.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A

LDLTLDMALGKKKNVEPFTSVLSLPYPFASEINKVAVFTENASEVKIAEENGAAFAGGTSLIQKIWDDEIVADFYVAVPEIMPELNRLRKKLNKKYPKLSRNSIGRDIPKMLELFKNGHEIKVDEERENFLQTKIATLDMSSDQIAANLQAVINEVCRHRPLNLGPFVVRAFLRSSTSEGLLLKIDPLL

Entity 3 polymer

描述 description	mitochondrial ribosomal protein L2
分子量 formula_weight	14442.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B

VIQVRYDPCRSADIALVAGGSRKRWIIATENMQAGDTILNSNHIGRMAVAAREGDAHPLGALPVGTLINNVESEPGRGAQYIRAAGTCGVLLRKVNGTAIIQLPSKRQMQVLETCVATVGRVSNVDHNKRVIGKAG

Entity 4 polymer

描述 description	Mitochondrial 39S ribosomal protein L3
分子量 formula_weight	23396.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

RVGLIALKLGMMPLWTKDGQKHVVTLLQVQDCHVLKYTSKENCNGKMATLSVGGKTVSRFRKATSILEFYRELGLPPKQTVKIFNITDNAAIKPGTPLYAAHFRPGQYVDVTAKTIGKGFQGVMKRWGFKGQPATHGQTKTHRRPGAVATGDIGRVWPGTKMPGKMGNIYRTEYGLKVWRINTKHNIIYVNGSVPGHKNCLVKVKDSKLPA

Entity 5 polymer

描述 description	mitochondrial ribosomal protein L4 isoform a
分子量 formula_weight	19707.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

HQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGLNVHSMLKHQTLVLTLPTVAFLED

Entity 6 polymer

描述 description	39S ribosomal protein L12, mitochondrial
分子量 formula_weight	14831.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E,F

PKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLMPMGGMVPGAAPAPTAPEAAEEDVPKQKERTHFTVRLTEAKPVDKVKLIKEIKNYVQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

Entity 7 polymer

描述 description	39S ribosomal protein L11, mitochondrial
分子量 formula_weight	15606.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G

KPEVGGVIRAIVRAGLAMPGPPLGPVLGQRGVSINQFCKEFNERTKDIKEGIPLPTKILVKPDRTFEIKIGQPTVSYFLKAAAGIEKGARQTGKEVAGLVTLKHVYEIARIKAQDEAFALQDVPLSSVVRSIIGSARSLGIRVVK

Entity 8 polymer

描述 description	39S ribosomal protein L13, mitochondrial
分子量 formula_weight	16958.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H

MSSFSRAPQQWATFARIWYLLDGKMQPPGKLAAMASIRLQGLHKPVYHALSDCGDHVVIMNTRHIAFSGNKWEQKVYSSHTGYPGGFRQVTAAQLHLRDPVAIVKLAIYGMLPKNLHRRTMMERLHLFPDEYIPEDILKNLVEELPQP

Entity 9 polymer

描述 description	Mitochondrial ribosomal protein L16
分子量 formula_weight	13107.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	I

DIRGPSTEATEFTEGNFAILALGGGYLHWGHFEMMRLTINRSMDPKNMFAIWRVPAPFKPITRKSVGHRMGGGKGAIDHYVTPVKAGRLVVEMGGRCEFEEVQGFLDQVAHKLPFAAK

Entity 10 polymer

描述 description	Mitochondrial ribosomal protein L17
分子量 formula_weight	13683.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	J

HGRVFRRMGLGPESRIHLLRNLLTGLVRHERIEAPWARVDEMRGYAEKLIDYGKLGDTNERAMRMADFWLTEKDLIPKLFQVLAPRYKDQTGGYTRMLQIPNRSLDRAKMAVIEYK

Entity 11 polymer

描述 description	39S ribosomal protein L19, mitochondrial
分子量 formula_weight	11098.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K

IPEFYVGSILRVTTADPYASGKISQFLGICIQRSGRGLGATFILRNVIEGQGVEICFELYNPRVQEIQVVKLEKRLDDSLLYLRDALPEYSTFDVNMK

Entity 12 polymer

描述 description	MRPL20 protein
分子量 formula_weight	14125.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L

LWLRNRVTDRYFRIQEVLKHARHFRGRKNRCYRLAVRTVIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPALIGNLVKCQVELNRKVLADLAIYEPKTFKSLAALASRRRH

Entity 13 polymer

描述 description	mitochondrial ribosomal protein L22 isoform a
分子量 formula_weight	12914.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

HCRRQIKYSKDKMWYLAKLIRGMSIDQALAQLEFNDKKGAKIIKEVLLEAQDMAVRDHNVEFRSNLYIAESTSGRGQCLKRIRYHGRGRFGIMEKVYCHYFVKLVEGPPP

Entity 14 polymer

描述 description	mitochondrial ribosomal protein L24
分子量 formula_weight	11010.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N

GDTVEILEGKDAGKQGKVVQVIRQRNWVVVGGLNTHYRYIGKTMDYRGTMIPSEAPLLHRQVKLVDPMDRKPTEIEWRFTEAGERVRVSTRSGRII

Entity 15 polymer

描述 description	Mitochondrial 39S ribosomal protein L27
分子量 formula_weight	7520.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O

ASKKSGGSSKNLGGKSSGRRQGIKKMEGHYVHAGNIIATQRHFRWHPGAHVGVGKNKCLYALEEGIVRY

Entity 16 polymer

描述 description	mitochondrial ribosomal protein L33 isoform a
分子量 formula_weight	6092.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	P

AKSKSKNILVRMVSEAGTGFCFNTKRNRLREKLTLLHYDPVVKQRVLFVEKK

Entity 17 polymer

描述 description	39S ribosomal protein L34, mitochondrial
分子量 formula_weight	4485.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Q

YQPSNIKRKNKHGWVRRLSTPAGVQVILRRMLKGRKSL

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7. 引用文献 (1)

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分辨率 resolution	12.10 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	—

fit	/app/data/saxs_computation/ft/2ftc/2ftc.fit
pddf	/app/data/pr_computation/ft/2ftc/2ftc.pr.out
profile	/app/data/saxs_computation/ft/2ftc/2ftc.dat

Structural Model for the Large Subunit of the Mammalian Mitochondrial Ribosome

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (17)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)