2hqt

;Crystal structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA aminoacylation and nuclear export cofactor Arc1p reveal a novel function for an old fold ;

Method: X-RAY DIFFRACTION Dmax: 140.8 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

SAXS profile for 2hqt

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2hqt
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2hqt
沉积日期 deposition_date2006-07-19
结构标题 title;Crystal structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA aminoacylation and nuclear export cofactor Arc1p reveal a novel function for an old fold ;
关键词 keywordsGST-fold, BIOSYNTHETIC PROTEIN, RNA BINDING; BIOSYNTHETIC PROTEIN, RNA BINDING
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier45.98
回转半径 Rg (电子) rg_electron45.70
零角强度 I(0) i0938911000.00
分子量 molecular_weight263500.0 kDa
排除体积 excluded_volume334000 ų
包络体积 envelope_volume486170 ų
水化壳体积 shell_volume87383 ų
包络直径 envelope_diameter150.2
壳层 Rg shell_rg51.48
包络 Rg envelope_rg44.62
形状 Rg shape_rg45.69
总 Rg total_rg46.01
总原子数 total_atoms18611
残基数 n_residues2356
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax140.8
Rg (实空间) rg_real45.81
Rg 误差 (实空间) rg_real_error0.95
I(0) (实空间) i0_real9.3890e+08
I(0) 误差 (实空间) i0_real_error1.6290e+07
Rg (倒空间) rg_reciprocal45.98
I(0) (倒空间) i0_reciprocal939100000.0000
解质量估计 total_estimate0.6397
解质量评级 solution_quality REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary53.8
偏度 Skewness skewness0.218
峰度 Kurtosis kurtosis-0.477
角度范围 angular_range— – 0.1700 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha74140000.0000
实空间数据点数 n_real_points35
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.947; Stabil: 1.000; Sysdev: 0.021; Positv: 1.000; Valcen: 0.985; Smooth: 0.423

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (3)

6. 折叠分类 (SCOP + CATH) 42 domains

SCOP 2.08 (22 domains)

结构域编号 domain_idd2hqta1
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtb_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtc_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtd_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqte_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtf2
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtf3
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd2hqtg_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqth_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqti_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtj2
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtj3
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd2hqtk_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtl_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtm_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtn_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqto_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtp_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtq_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtr_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqts_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like
结构域编号 domain_idd2hqtt_
类 Class classa — All alpha proteins
折叠类型 Fold folda.45 — GST C-terminal domain-like
超家族 Superfamily superfamilya.45.1 — GST C-terminal domain-like
家族 Family familya.45.1.2 — Arc1p N-terminal domain-like

CATH v4.4 (20 domains)

结构域编号 domain_id2hqtA00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtB00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtC00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtD00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtE00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtF00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtG00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtH00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtI00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtJ00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtK00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtL00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtM00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtN00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtO00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtP00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtQ00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtR00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtS00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id2hqtT00
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1050 — Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2
同源超家族 H-superfamily homologous superfamily10

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8. 文件与曲线 (10)