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2hzs

Structure of the Mediator head submodule Med8C/18/20

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

RNA polymerase II mediator complex subunit 20

Saccharomyces cerevisiae

UniProt P34162

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 18 × 1 （P32585） RNA polymerase II mediator complex subunit 8 × 1 （P38304） water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 18 × 1 （P32585） RNA polymerase II mediator complex subunit 8 × 1 （P38304） water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 18 × 1 （P32585） RNA polymerase II mediator complex subunit 8 × 1 （P38304） water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 18 × 1 （P32585） RNA polymerase II mediator complex subunit 8 × 1 （P38304） water × 1 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 12 RNA polymerase II mediator complex subunit 18 × 4 （P32585） RNA polymerase II mediator complex subunit 8 × 4 （P38304） water × 8 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 12 RNA polymerase II mediator complex subunit 18 × 4 （P32585） RNA polymerase II mediator complex subunit 8 × 4 （P38304） water × 8 Consistent with protein count
7 Protein heterocomplex Heteromer Protein 9 RNA polymerase II mediator complex subunit 18 × 3 （P32585） RNA polymerase II mediator complex subunit 8 × 3 （P38304） water × 6 Consistent with protein count
8 Protein heterocomplex Heteromer Protein 6 RNA polymerase II mediator complex subunit 18 × 2 （P32585） RNA polymerase II mediator complex subunit 8 × 2 （P38304） water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2hzm 2hzm 2hzm 2hzm 2hzs 2hzs 2hzs 2hzs 2hzs 2hzs 2hzs 2hzs

26 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name MED20_YEAST
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–209； UniProt 2–210 Author chain C； PDBConstruct 1–209； UniProt 2–210 Author chain E； PDBConstruct 1–209； UniProt 2–210 Author chain G； PDBConstruct 1–209； UniProt 2–210

RNA polymerase II mediator complex subunit 18

Saccharomyces cerevisiae

UniProt P32585

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 20 × 1 （P34162） RNA polymerase II mediator complex subunit 8 × 1 （P38304） water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 20 × 1 （P34162） RNA polymerase II mediator complex subunit 8 × 1 （P38304） water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 20 × 1 （P34162） RNA polymerase II mediator complex subunit 8 × 1 （P38304） water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 20 × 1 （P34162） RNA polymerase II mediator complex subunit 8 × 1 （P38304） water × 1 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 12 RNA polymerase II mediator complex subunit 20 × 4 （P34162） RNA polymerase II mediator complex subunit 8 × 4 （P38304） water × 8 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 12 RNA polymerase II mediator complex subunit 20 × 4 （P34162） RNA polymerase II mediator complex subunit 8 × 4 （P38304） water × 8 Consistent with protein count
7 Protein heterocomplex Heteromer Protein 9 RNA polymerase II mediator complex subunit 20 × 3 （P34162） RNA polymerase II mediator complex subunit 8 × 3 （P38304） water × 6 Consistent with protein count
8 Protein heterocomplex Heteromer Protein 6 RNA polymerase II mediator complex subunit 20 × 2 （P34162） RNA polymerase II mediator complex subunit 8 × 2 （P38304） water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2hzm 2hzm 2hzm 2hzm 2hzs 2hzs 2hzs 2hzs 2hzs 2hzs 2hzs 2hzs

25 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name MED18_YEAST
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–306； UniProt 2–307 Author chain D； PDBConstruct 1–306； UniProt 2–307 Author chain F； PDBConstruct 1–306； UniProt 2–307 Author chain H； PDBConstruct 1–306； UniProt 2–307

RNA polymerase II mediator complex subunit 8

Saccharomyces cerevisiae

UniProt P38304

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 20 × 1 （P34162） RNA polymerase II mediator complex subunit 18 × 1 （P32585） water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 20 × 1 （P34162） RNA polymerase II mediator complex subunit 18 × 1 （P32585） water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 20 × 1 （P34162） RNA polymerase II mediator complex subunit 18 × 1 （P32585） water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 3 RNA polymerase II mediator complex subunit 20 × 1 （P34162） RNA polymerase II mediator complex subunit 18 × 1 （P32585） water × 1 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 12 RNA polymerase II mediator complex subunit 20 × 4 （P34162） RNA polymerase II mediator complex subunit 18 × 4 （P32585） water × 8 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 12 RNA polymerase II mediator complex subunit 20 × 4 （P34162） RNA polymerase II mediator complex subunit 18 × 4 （P32585） water × 8 Consistent with protein count
7 Protein heterocomplex Heteromer Protein 9 RNA polymerase II mediator complex subunit 20 × 3 （P34162） RNA polymerase II mediator complex subunit 18 × 3 （P32585） water × 6 Consistent with protein count
8 Protein heterocomplex Heteromer Protein 6 RNA polymerase II mediator complex subunit 20 × 2 （P34162） RNA polymerase II mediator complex subunit 18 × 2 （P32585） water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2hzs 2hzs 2hzs 2hzs 2hzs 2hzs 2hzs 2hzs 3j1o 3rj1 3rj1 3rj1

21 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name MED8_YEAST
Isoform —
PDB entities 3
Chains and sequence ranges Author chain I； PDBConstruct 1–21； UniProt 189–209 Author chain J； PDBConstruct 1–21； UniProt 189–209 Author chain K； PDBConstruct 1–21； UniProt 189–209 Author chain L； PDBConstruct 1–21； UniProt 189–209

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2hzs
Deposition date deposition_date2006-08-09
Structure title titleStructure of the Mediator head submodule Med8C/18/20
Keywords keywordsbeta barrel, channel, helix, TRANSCRIPTION; TRANSCRIPTION
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2hzs__assembly_5__model_1

Assembly 5 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2hzs__assembly_5__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2hzs__assembly_5__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）43.61 Å
Rg (electron density)43.24 Å
Total Rg43.47 Å
Atom count14001
Residues1799
Excluded volume250570 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2hzs__assembly_1__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 2hzs__assembly_2__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 2hzs__assembly_3__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 2hzs__assembly_4__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 2hzs__assembly_5__model_1 dodecameric （12） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 2hzs__assembly_6__model_1 dodecameric （12） Success 4.1.3-1-20251215 (887e7ef) View Download
7 1 2hzs__assembly_7__model_1 nonameric （9） Success 4.1.3-1-20251215 (887e7ef) View Download
8 1 2hzs__assembly_8__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

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6. Fold Classification (SCOP + CATH) 12 domains

CATH v4.4 (12 domains)

Domain ID domain_id2hzsA01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology310 — TATA-Binding Protein
Homologous superfamily homologous superfamily180 —
Domain ID domain_id2hzsA02
Class class2 — Mainly Beta
Architecture architecture20 — Single Sheet
Topology topology140 — q64v53_bacfr protein fold
Homologous superfamily homologous superfamily20 —
Domain ID domain_id2hzsB01
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology320 — Hypothetical Protein Pfu-838710-001
Homologous superfamily homologous superfamily10 — Hypothetical Protein Pfu-838710-001
Domain ID domain_id2hzsC01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology310 — TATA-Binding Protein
Homologous superfamily homologous superfamily180 —
Domain ID domain_id2hzsC02
Class class2 — Mainly Beta
Architecture architecture20 — Single Sheet
Topology topology140 — q64v53_bacfr protein fold
Homologous superfamily homologous superfamily20 —
Domain ID domain_id2hzsD01
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology320 — Hypothetical Protein Pfu-838710-001
Homologous superfamily homologous superfamily10 — Hypothetical Protein Pfu-838710-001
Domain ID domain_id2hzsE01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology310 — TATA-Binding Protein
Homologous superfamily homologous superfamily180 —
Domain ID domain_id2hzsE02
Class class2 — Mainly Beta
Architecture architecture20 — Single Sheet
Topology topology140 — q64v53_bacfr protein fold
Homologous superfamily homologous superfamily20 —
Domain ID domain_id2hzsF00
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology320 — Hypothetical Protein Pfu-838710-001
Homologous superfamily homologous superfamily10 — Hypothetical Protein Pfu-838710-001
Domain ID domain_id2hzsG01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology310 — TATA-Binding Protein
Homologous superfamily homologous superfamily180 —
Domain ID domain_id2hzsG02
Class class2 — Mainly Beta
Architecture architecture20 — Single Sheet
Topology topology140 — q64v53_bacfr protein fold
Homologous superfamily homologous superfamily20 —
Domain ID domain_id2hzsH01
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology320 — Hypothetical Protein Pfu-838710-001
Homologous superfamily homologous superfamily10 — Hypothetical Protein Pfu-838710-001
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7. Citations (1)