PROTEIN G
Streptococcus sp. GX7805
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 367–430 | Not recorded | No other associated polymer | SOLUTION NMR mmCIF provides none of the parsed experimental conditions | Resolution not provided |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 2IGG | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 10DV Room Temperature X-Ray Structure of SARS CoV-2 Main Protease Intermediate Precursor with Ensitrelvir (ESV) Deposited 2026-01-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
304–357（54 aa）
Chain B
304–357（54 aa）
|
Mutation：C145A Mutation：C145A | 7YY 6-[(6-chloranyl-2-methyl-indazol-5-yl)amino]-3-[(1-methyl-1,2,4-triazol-3-yl)methyl]-1-[[2,4,5-tris(fluoranyl)phenyl]methyl]-1,3,5-triazine-2,4-dione × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；18-21% PEG3350, 0.1 M Bis-Tris, pH 6.5 or 7.0
|
Resolution 2.05 Å R-free 0.234 |
| 1FCC CRYSTAL STRUCTURE OF THE C2 FRAGMENT OF STREPTOCOCCAL PROTEIN G IN COMPLEX WITH THE FC DOMAIN OF HUMAN IGG Deposited 1995-01-17 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
372–427（56 aa）
Chain D
372–427（56 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.20 Å R-free 0.357 |
| 1FCL DELTA1.5: A COMPUTATIONALLY DESIGNED CORE VARIANT OF THE B1 DOMAIN OF STREPTOCOCCAL PROTEIN G Deposited 2000-07-18 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
302–357（56 aa）
Fragment：GB1_DELTA1.5
|
Mutation：Y3F, L7I, F30L, A34I, V39I, F52W | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6；298 K；Ionic strength (raw mmCIF value) 50 mM sodium phosphate；Pressure Atmospheric
NMR sample composition
1 mM unlabeled protein in 50 mM sodium phosphate, pH 6.0 | either D2O or 90:10 H2O:D2O
|
Resolution not provided |
| 1FD6 DELTA0: A COMPUTATIONALLY DESIGNED CORE VARIANT OF THE B1 DOMAIN OF STREPTOCOCCAL PROTEIN G Deposited 2000-07-19 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
302–357（56 aa）
Fragment：GB1_DELT0
|
Mutation：Y3F, L7I, V39I | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6；298 K；Ionic strength (raw mmCIF value) 50 mM sodium phosphate；Pressure ambient
NMR sample composition
3 mM protein in 50 mM sodium phosphate, pH 6.0 | either D2O or 90:10 H2O:D2O
|
Resolution not provided |
| 1GB4 HYPERTHERMOPHILIC VARIANT OF THE B1 DOMAIN FROM STREPTOCOCCAL PROTEIN G, NMR, 47 STRUCTURES Deposited 1998-01-19 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
372–427（56 aa）
Fragment：B1 DOMAIN
|
Mutation：V1M, Y4F, V7I, T17I, T19I, T26E, V30I, V40I | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5；308 K；Ionic strength (raw mmCIF value) 50mM；Pressure ATMOSPHERIC
NMR sample composition
H2O/D2O
|
Resolution not provided |
| 1GJS Solution structure of the Albumin binding domain of Streptococcal Protein G Deposited 2001-08-02 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
254–299（46 aa）
Fragment：ALBUMIN-BINDING DOMAIN RESIDUES 254-299
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.2；300 K；Pressure 1
|
Resolution not provided |
| 1GJT Solution structure of the Albumin binding domain of Streptococcal Protein G Deposited 2001-08-02 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
254–299（46 aa）
Fragment：ALBUMIN-BINDING DOMAIN
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.2；300 K；Pressure 1
|
Resolution not provided |
| 1IBX NMR STRUCTURE OF DFF40 AND DFF45 N-TERMINAL DOMAIN COMPLEX Deposited 2001-03-29 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
302–357（56 aa）
Fragment：B1 DOMAIN OF PROTEIN G FUSED WITH N-TERMINAL DOMAIN (CIDE DOMAIN) OF DFF45
|
Mutation：T(-43)Q | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6；296 K；Ionic strength (raw mmCIF value) 50 mM NaCl；Pressure ambient
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex 90% deuterated, U-15N/13C labeled DFF40 non-labeled DFF45 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl 10%D2O/90%H2O
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex U-13C labeled DFF45 non-labeled DFF40 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl D2O
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex 90% deuterated, U-15N/13C labeled DFF45 non-labeled DFF40 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl 10%D2O/90%H2O
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex U-15N labeled DFF40 non-labeled DFF45 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl 10%D2O/90%H2O
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex U-15N labeled DFF45 non-labeled DFF40 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl 10%D2O/90%H2O
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex perdeuterated, U-15N labeled DFF40 non-labeled DFF45 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl 10%D2O/90%H2O
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex perdeuterated, U-15N labeled DFF45 non-labeled DFF40 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl 10%D2O/90%H2O
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex 10% 13C labeled DFF40 non-labeled DFF45 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl 10%D2O/90%H2O
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex 10% 13C labeled DFF45 non-labeled DFF40 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl 10%D2O/90%H2O
NMR sample composition
0.6 mM DFF40/DFF45 NTD complex U-13C labeled DFF40 non-labeled DFF45 | 20 mM Phosphate buffer 5 mM DTT, 50 mM NaCl D2O
|
Resolution not provided |
| 1P7E GB3 solution structure obtained by refinement of X-ray structure with dipolar couplings Deposited 2003-05-01 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
444–497（54 aa）
Fragment：THIRD IGG-BINDING DOMAIN
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.5；300 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition
25 mM NaH2PO4/Na2HPO4, 0.2 mg/mL NaN3, 1.5 mM GB3 protein and aligning media
|
Resolution not provided |
| 1P7F GB3 solution structure obtained by refinement of X-ray structure with dipolar couplings Deposited 2003-05-01 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
444–497（54 aa）
Fragment：THIRD IGG-BINDING DOMAIN
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.5；300 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition
25 mM NaH2PO4/Na2HPO4, 0.2 mg/mL NaN3, 1.5 mM GB3 protein and aligning media
|
Resolution not provided |
| 1ZXH G311 mutant protein Deposited 2005-06-08 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
443–497（55 aa）
|
Mutation：yes | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；275 K；Ionic strength (raw mmCIF value) 0.1M；Pressure ambient
NMR sample composition
G311 mutant protein, 0.1 M KPi, pH7.0, ~0.6 M GuHCl. | 0.1 M KPi, pH7.0, ~0.6 M GuHCl.
|
Resolution not provided |
| 2GI9 Backbone Conformational Constraints in a Microcrystalline U-15N-Labeled Protein by 3D Dipolar-Shift Solid-State NMR Spectroscopy Deposited 2006-03-28 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
|
Mutation：T2Q | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4.5；5mM Acetate 3.8, 150mM NaCl, 50% MPD and 20% IPA, pH 4.5
|
Resolution 1.14 Å R-free 0.181 |
| 2I2Y Solution structure of the RRM of SRp20 bound to the RNA CAUC Deposited 2006-08-17 | Different construct Different oligomeric state Different experimental conditions | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: dimeric |
Chain A
304–357（54 aa）
Fragment：RRM domain
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.4；315 K；Pressure ambient
NMR sample composition
1mM SRp20 13C-15N labelled + 1 mM CAUC natural abundance in 50 mM sodium dihydrogenophosphate, pH 6.4, 1mM DTT; | 10% D2O,90% H2O
or 100% D2O
NMR sample composition
1mM SRp20 15N labelled + 1 mM CAUC natural abundance in 1mM SRp20 15N labelled in 50 mM sodium dihydrogenophosphate, pH 6.4, 1mM DTT | 10% D2O, 90% H2O
or 100% D2O
NMR sample composition
1mM SRp20 15N labelled + 1 mM CAUC with 13C labelled riboses and natural abundance bases in 50 mM sodium dihydrogenophosphate, pH 6.4, 1mM DTT; | 10% D2O, 90% H2O
or 100% D2O
NMR sample composition
1mM SRp20 15N labelled + 1 mM CAUC with A2 and U3 riboses 13C labelled and natural abundance of other riboses and all bases in 50 mM sodium dihydrogenophosphate, pH 6.4, 1mM DTT; | 10% D2O, 90% H2O
or 100% D2O
NMR sample composition
1mM SRp20 15N labelled + 1 mM CAUC with C1 and C4 riboses 13C labelled and natural abundance of other riboses and all bases, in 50 mM sodium dihydrogenophosphate, pH 6.4, 1mM DTT; | 10% D2O, 90% H2O
or 100% D2O
|
Resolution not provided |
| 2I38 Solution structure of the RRM of SRp20 Deposited 2006-08-17 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
Fragment：RRM domain
|
Mutation：E129D | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5.8；315 K；Pressure ambient
NMR sample composition
1mM SRp20 13C-15N labelled in 50 mM sodium dihydrogenophosphate, pH 5.8, 1mM DTT; | 10% D2O, 90% H2O
or 100% D2O
NMR sample composition
1mM SRp20 15N labelled in 50 mM sodium dihydrogenophosphate, pH 5.8, 1mM DTT; | 10% D2O, 90% H2O
or 100% D2O
|
Resolution not provided |
| 2JSV Dipole tensor-based refinement for atomic-resolution structure determination of a nanocrystalline protein by solid-state NMR spectroscopy Deposited 2007-07-16 | Different construct Different mutation/modification Different experimental method Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
303–357（55 aa）
Fragment：2-1 repeat
|
Mutation：T2Q | No recorded non-water small molecule |
SOLID-STATE NMR
NMR measurement conditions
pH 5.5；281 K；Pressure ambient
NMR sample composition
14 mg/mL [U-99% 13C; U-99% 15N] protein, (4R)-2-Metylpentane-2,4-Diol (50% v/v), Isopropyl alcohol (25% v/v), 25 mg/mL GB1 in 50 mM sodium phosphate buffered H2O | H2O
NMR sample composition
14 mg/mL [U-99% 15N] protein, (4R)-2-Metylpentane-2,4-Diol (50% v/v), Isopropyl alcohol (25% v/v), 25 mg/mL GB1 in 50 mM sodium phosphate buffered H2O | H2O
NMR sample composition
14 mg/mL [U-100% 13C; U-100% 15N] protein, (4R)-2-Metylpentane-2,4-Diol (50% v/v), Isopropyl alcohol (25% v/v), 25 mg/mL GB1 in 50 mM sodium phosphate buffered H2O | H2O
NMR sample composition
14 mg/mL (1,3) 13C glycerol, U15N protein, (4R)-2-Metylpentane-2,4-Diol (50% v/v), Isopropyl alcohol (25% v/v), 25 mg/mL GB1 in 50 mM sodium phosphate buffered H2O | H2O
NMR sample composition
14 mg/mL 2 13C glycerol, Uniform 15N protein, (4R)-2-Metylpentane-2,4-Diol (50% v/v), Isopropyl alcohol (25% v/v), 25 mg/mL GB1 in 50 mM sodium phosphate buffered H2O | H2O
|
Resolution not provided |
| 2KHU Solution Structure of the Ubiquitin-Binding Motif of Human Polymerase Iota Deposited 2009-04-11 | Different construct Different oligomeric state Different experimental conditions | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
Fragment：UNP residues 304-357, 676-715
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；25 K；Ionic strength (raw mmCIF value) 0.1；Pressure ambient
NMR sample composition
1-4 mM [U-100% 13C; U-100% 15N] D2O-1, 1-4 mM [U-100% 13C; U-100% 15N] H2O-2, 100% D2O | 100% D2O
|
Resolution not provided |
| 2KHW Solution Structure of the human Polymerase iota UBM2-Ubiquitin Complex Deposited 2009-04-13 | Different construct Different oligomeric state Different experimental conditions | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
304–357（54 aa）
Fragment：UNP residues 304-357, 676-715
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition
1 mM [U-100% 15N] entity_1-1, 4 mM entity_2-2, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
3 mM [U-100% 15N] entity_1-3, 3 mM [U-100% 15N] entity_2-4, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
3 mM [U-100% 13C; U-100% 15N] entity_1-5, 3 mM [U-100% 13C; U-100% 15N] entity_2-6, 100% D2O | 100% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] entity_1-7, 4 mM entity_2-8, 100% D2O | 100% D2O
|
Resolution not provided |
| 2KN4 The structure of the RRM domain of SC35 Deposited 2009-08-14 | Different construct Different oligomeric state Different experimental conditions | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
Fragment：UNP residues 304-357 from P19909, UNP residues 9-101 from Q01130
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.8；305 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition
0.5 mM [U-100% 13C; U-100% 15N] SC35-1, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM [U-100% 13C; U-100% 15N] SC35-2, 100% D2O | 100% D2O
NMR sample composition
0.5 mM [U-100% 15N] SC35-3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM SC35-4, 100% D2O | 100% D2O
|
Resolution not provided |
| 2KQ4 Atomic resolution protein structure determination by three-dimensional transferred echo double resonance solid-state nuclear magnetic resonance spectroscopy Deposited 2009-10-27 | Different construct Different mutation/modification Different experimental method Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
303–357（55 aa）
Fragment：UNP residues 303 to 357
|
Mutation：T2Q | No recorded non-water small molecule |
SOLID-STATE NMR
NMR measurement conditions
Pressure ambient
NMR sample composition
30 mg 1,3-13C glycerol, U 15N entity-1, 50 v/v MPTG-2, solid | solid
NMR sample composition
30 mg 2-13C glycerol, U 15N entity-3, 50 v/v MPTG-4, solid | solid
|
Resolution not provided |
| 2KWD Supramolecular Protein Structure Determination by Site-Specific Long-Range Intermolecular Solid State NMR Spectroscopy Deposited 2010-04-05 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
304–357（54 aa）
Chain B
304–357（54 aa）
Chain C
304–357（54 aa）
Chain D
304–357（54 aa）
Chain E
304–357（54 aa）
|
Mutation：T2Q Mutation：T2Q Mutation：T2Q Mutation：T2Q Mutation：T2Q | No recorded non-water small molecule |
SOLID-STATE NMR
NMR measurement conditions
273 K；Pressure 1
NMR sample composition
20 mg [U-1,3-13C-glycerol; U-100% 15N] GB1-1, solid | solid
NMR sample composition
50 % [U-1,3-13C-glycerol] GB1-2, 50 % [U-99% 15N] GB1-3, solid | solid
NMR sample composition
20 mg [U-2-13C-glycerol; U-100% 15N] GB1-4, solid | solid
|
Resolution not provided |
| 2LUM Three-State Ensemble obtained from eNOEs of the Third Immunoglobulin Binding Domain of Protein G (GB3) Deposited 2012-06-18 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
444–497（54 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition
4 mM [U-95% 13C; U-95% 15N] Third Immunoglobulin G-binding protein G, 50 mM potassium phosphate, 0.5 mg/mL sodium azide, 50 mM sodium chloride, 97% H2O/3% D2O | 97% H2O/3% D2O
|
Resolution not provided |
| 2N9K 1H, 13C, and 15N Chemical Shift Assignments for in vitro GB1 Deposited 2015-11-26 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
Fragment：UNP residues 303-357
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；295 K；Ionic strength (raw mmCIF value) 0.31；Pressure ambient
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] Protein G B1-1, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2N9L 1H, 13C, and 15N Chemical Shift Assignments for in-cell GB1 Deposited 2015-11-30 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
Fragment：UNP residues 303-357
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；295 K；Ionic strength (raw mmCIF value) 0.31；Pressure ambient
NMR sample composition
250 uM [U-100% 13C; U-100% 15N] Protein G B1-1, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2OED GB3 solution structure obtained by refinement of X-ray structure with dipolar couplings Deposited 2006-12-29 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
444–497（54 aa）
Fragment：THIRD IGG-BINDING DOMAIN
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.5；300 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition
25 MM NAH2PO4/NA2HPO4, 0.2 MG/ML NAN3, 1.5 MM GB3 PROTEIN AND ALIGNING MEDIA, 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2ON8 Gbeta1 stabilization by in vitro evolution and computational design Deposited 2007-01-23 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
373–427（55 aa）
|
Mutation：T1M, T2Q, Y3F, V6I, T16I, T18L, T25E, V29K, V39I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；50mM Na acetate, pH 5.0, 17 mg/ml protein mixed with equal volume of 2.3M ammonium suphate, 0.1M sodium acetate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 1.35 Å R-free 0.195 |
| 2ONQ Gbeta1 stabilization by in vitro evolution and computational design Deposited 2007-01-24 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
373–427（55 aa）
Fragment：residues 373-427
|
Mutation：T2Q, Y3F, L7I, T16I, T18I, T25E, V29F, V39I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293.15 K；50mM sodium acetate, 20mg/ml protein solution mixed in equal volume of crystallization buffer containing
33% PEG monomethylether, 0.1M calcium chloride, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 1.70 Å R-free 0.229 |
| 2PLP Ultra high resolution backbone conformation of protein GB1 from residual dipolar couplings alone Deposited 2007-04-20 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–356（54 aa）
Fragment：First Immunoglobin binding domain (GB1)
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5.6；300 K；Ionic strength (raw mmCIF value) 50mM salt；Pressure ambient
NMR sample composition
perdeuterated (triple labelled 15N, 13C, 2D) | Partially aligned in bacteriophage and lyotrophic alcohol medium.
|
Resolution not provided |
| 2QMT Crystal Polymorphism of Protein GB1 Examined by Solid-state NMR and X-ray Diffraction Deposited 2007-07-16 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
Fragment：immunoglobulin beta 1 binding domain (residues 303-357)
|
Mutation：T2Q | PO4 PHOSPHATE ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；50 mM NaCl, 50% MPD, 6% IPA, 25 mM Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.05 Å R-free 0.207 |
| 3FIL Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution Deposited 2008-12-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
303–357（55 aa）
Fragment：immunoglobulin binding domain, UNP residues 303-357
Chain B
303–357（55 aa）
Fragment：immunoglobulin binding domain, UNP residues 303-357
|
Mutation：T2Q, E15V, T16L, T18I, N37L Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：T2Q, E15V, T16L, T18I, N37L | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293.15 K；protein solution: 50mM sodium acetate pH 5.6, 50mg/ml protein. reservoir solution: 25% PEG 3350, 0.1M citric acids pH 3.5. Drop 400nl protein solution & 400nl reservoir solution, 80 micro-l reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
Resolution 0.88 Å R-free 0.149 |
| 3UI3 Structural and Biochemical Characterization of HP0315 from Helicobacter pylori as a VapD Protein with an Endoribonuclease Activity Deposited 2011-11-04 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
304–357（54 aa）
Chain B
304–357（54 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；0.2M (NH4)2SO4, 9% PEG3350, 5-8% glycerol, 100mM 2-(N-morpholino)ethanesulfonic acid', pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.284 |
| 3V3X Nitroxide Spin Labels in Protein GB1: N8/K28 Double Mutant Deposited 2011-12-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
304–357（54 aa）
Chain C
304–357（54 aa）
|
Mutation：C8N Mutation：C8N | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 3 2PE NONAETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；150 mM sodium acetate pH 4.5, 18% w/v PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.288 |
| 3V3X Nitroxide Spin Labels in Protein GB1: N8/K28 Double Mutant Deposited 2011-12-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
304–357（54 aa）
Chain D
304–357（54 aa）
|
Mutation：C8N Mutation：C8N | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 4 GOL GLYCEROL × 1 ACT ACETATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；150 mM sodium acetate pH 4.5, 18% w/v PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.288 |
| 4WH4 Protein GB1 Quadruple Mutant I6H/N8H/K28H/Q32H Deposited 2014-09-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Mutation：I6H/N8H/K28H/Q32H | SO4 SULFATE ION × 1 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；1.75 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M HEPES pH 7.5
|
Resolution 2.20 Å R-free 0.256 |
| 4WH4 Protein GB1 Quadruple Mutant I6H/N8H/K28H/Q32H Deposited 2014-09-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
304–357（54 aa）
|
Mutation：I6H/N8H/K28H/Q32H | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；1.75 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M HEPES pH 7.5
|
Resolution 2.20 Å R-free 0.256 |
| 5BMG Nitroxide Spin Labels in Protein GB1: E15 Mutant Deposited 2015-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：E15C | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；0.1 M magnesium chloride, 0.1 M Tris pH 4.5, 20% w/v PEG 4000
|
Resolution 2.20 Å R-free 0.218 |
| 5BMG Nitroxide Spin Labels in Protein GB1: E15 Mutant Deposited 2015-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：E15C | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；0.1 M magnesium chloride, 0.1 M Tris pH 4.5, 20% w/v PEG 4000
|
Resolution 2.20 Å R-free 0.218 |
| 5BMG Nitroxide Spin Labels in Protein GB1: E15 Mutant Deposited 2015-05-22 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：E15C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；0.1 M magnesium chloride, 0.1 M Tris pH 4.5, 20% w/v PEG 4000
|
Resolution 2.20 Å R-free 0.218 |
| 5BMG Nitroxide Spin Labels in Protein GB1: E15 Mutant Deposited 2015-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：E15C | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；0.1 M magnesium chloride, 0.1 M Tris pH 4.5, 20% w/v PEG 4000
|
Resolution 2.20 Å R-free 0.218 |
| 5BMG Nitroxide Spin Labels in Protein GB1: E15 Mutant Deposited 2015-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：E15C | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；0.1 M magnesium chloride, 0.1 M Tris pH 4.5, 20% w/v PEG 4000
|
Resolution 2.20 Å R-free 0.218 |
| 5BMG Nitroxide Spin Labels in Protein GB1: E15 Mutant Deposited 2015-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：E15C | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；0.1 M magnesium chloride, 0.1 M Tris pH 4.5, 20% w/v PEG 4000
|
Resolution 2.20 Å R-free 0.218 |
| 5BMG Nitroxide Spin Labels in Protein GB1: E15 Mutant Deposited 2015-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain G
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：E15C | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；0.1 M magnesium chloride, 0.1 M Tris pH 4.5, 20% w/v PEG 4000
|
Resolution 2.20 Å R-free 0.218 |
| 5BMG Nitroxide Spin Labels in Protein GB1: E15 Mutant Deposited 2015-05-22 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain H
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：E15C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；0.1 M magnesium chloride, 0.1 M Tris pH 4.5, 20% w/v PEG 4000
|
Resolution 2.20 Å R-free 0.218 |
| 5BMH Nitroxide Spin Labels in Protein GB1: T44 Mutant, Crystal Form B Deposited 2015-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：T44C | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；0.2 M potassium sodium tartrate, 0.2 M sodium citrate pH 6.0, 2 M ammonium sulfate
|
Resolution 1.60 Å R-free 0.175 |
| 5BMI Nitroxide Spin Labels in Protein GB1: T44 Mutant, Crystal Form A Deposited 2015-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
Fragment：UNP residues 304-357
|
Mutation：T44C | MTN S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1 M HEPES pH 7.5, 1.2 M sodium citrate
|
Resolution 2.50 Å R-free 0.251 |
| 5HFY Backbone Modifications in the Protein GB1 Helix: beta-2-Ala24, beta-3-Lys28, beta-3-Lys31, beta-3-Asn35 Deposited 2016-01-07 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；0.2 M sodium acetate pH 4.6, 20% w/v PEG 4000
|
Resolution 1.95 Å R-free 0.230 |
| 5HFY Backbone Modifications in the Protein GB1 Helix: beta-2-Ala24, beta-3-Lys28, beta-3-Lys31, beta-3-Asn35 Deposited 2016-01-07 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；0.2 M sodium acetate pH 4.6, 20% w/v PEG 4000
|
Resolution 1.95 Å R-free 0.230 |
| 5HG2 Backbone Modifications in the Protein GB1 Helix: beta-3-Ala24, beta-3-Lys28, beta-3-Lys31, beta-2-Asn35 Deposited 2016-01-07 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1 M sodium cacodylate pH 6.5, 0.1 M magnesium acetate, 20% w/v PEG 4000
|
Resolution 1.80 Å R-free 0.216 |
| 5HG2 Backbone Modifications in the Protein GB1 Helix: beta-3-Ala24, beta-3-Lys28, beta-3-Lys31, beta-2-Asn35 Deposited 2016-01-07 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1 M sodium cacodylate pH 6.5, 0.1 M magnesium acetate, 20% w/v PEG 4000
|
Resolution 1.80 Å R-free 0.216 |
| 5HG2 Backbone Modifications in the Protein GB1 Helix: beta-3-Ala24, beta-3-Lys28, beta-3-Lys31, beta-2-Asn35 Deposited 2016-01-07 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1 M sodium cacodylate pH 6.5, 0.1 M magnesium acetate, 20% w/v PEG 4000
|
Resolution 1.80 Å R-free 0.216 |
| 5HG2 Backbone Modifications in the Protein GB1 Helix: beta-3-Ala24, beta-3-Lys28, beta-3-Lys31, beta-2-Asn35 Deposited 2016-01-07 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1 M sodium cacodylate pH 6.5, 0.1 M magnesium acetate, 20% w/v PEG 4000
|
Resolution 1.80 Å R-free 0.216 |
| 5HI1 Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35 Deposited 2016-01-11 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M ammonium sulfate, 0.1M sodium acetate pH 4.5, 20% (w/v) PEG 4000
|
Resolution 2.15 Å R-free 0.252 |
| 5HI1 Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35 Deposited 2016-01-11 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M ammonium sulfate, 0.1M sodium acetate pH 4.5, 20% (w/v) PEG 4000
|
Resolution 2.15 Å R-free 0.252 |
| 5HI1 Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35 Deposited 2016-01-11 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M ammonium sulfate, 0.1M sodium acetate pH 4.5, 20% (w/v) PEG 4000
|
Resolution 2.15 Å R-free 0.252 |
| 5HI1 Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35 Deposited 2016-01-11 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M ammonium sulfate, 0.1M sodium acetate pH 4.5, 20% (w/v) PEG 4000
|
Resolution 2.15 Å R-free 0.252 |
| 5HI1 Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35 Deposited 2016-01-11 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M ammonium sulfate, 0.1M sodium acetate pH 4.5, 20% (w/v) PEG 4000
|
Resolution 2.15 Å R-free 0.252 |
| 5HI1 Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35 Deposited 2016-01-11 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M ammonium sulfate, 0.1M sodium acetate pH 4.5, 20% (w/v) PEG 4000
|
Resolution 2.15 Å R-free 0.252 |
| 5HI1 Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35 Deposited 2016-01-11 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain G
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M ammonium sulfate, 0.1M sodium acetate pH 4.5, 20% (w/v) PEG 4000
|
Resolution 2.15 Å R-free 0.252 |
| 5HI1 Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35 Deposited 2016-01-11 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain H
302–357（56 aa）
Fragment：UNP residues 302-357
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M ammonium sulfate, 0.1M sodium acetate pH 4.5, 20% (w/v) PEG 4000
|
Resolution 2.15 Å R-free 0.252 |
| 5LDE Crystal structure of a vFLIP-IKKgamma stapled peptide dimer Deposited 2016-06-24 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
304–356（53 aa）
Fragment：UNP residues 304-356
Chain B
304–356（53 aa）
Fragment：UNP residues 304-356
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；299 K；1.2M Ammonium Sulphate,
0.05M tri-sodium citrate,
3% isopropanol,
0.1-0.2% vitamin B12
|
Resolution 3.38 Å R-free 0.290 |
| 5UB0 Solution NMR Structure of NERD-C, a natively folded tetramutant of the B1 domain of streptococcal protein G (GB1) Deposited 2016-12-20 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
373–427（55 aa）
Fragment：UNP residues 373-427
|
Mutation：Y3F/L7I/F30L/V39I | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 10 mM sodium phosphate；Pressure 1
NMR sample composition
1.0 mM [U-98% 15N] protein (GB1), 10 mM sodium phosphate, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
1.0 mM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.0 mM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100% D2O | 100% D2O
|
Resolution not provided |
| 5UBS Solution NMR Structure of NERD-S, a natively folded pentamutant of the B1 domain of streptococcal protein G (GB1) with a solvent-exposed Trp43 Deposited 2016-12-21 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
373–427（55 aa）
Fragment：UNP residues 373-427
|
Mutation：Y3F/L7I/A34F/V39L/V54I | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 10 mM sodium phosphate；Pressure 1
NMR sample composition
1 mM [U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 100% D2O | 100% D2O
|
Resolution not provided |
| 5UCE Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1) Deposited 2016-12-22 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
373–427（55 aa）
Fragment：UNP residues 373-427
|
Mutation：Y3F/L5A/L7I/A34F/V39L | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 10 mM sodium phosphate；Pressure 1
NMR sample composition
200 uM [U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
200 uM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
200 uM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 100% D2O | 100% D2O
|
Resolution not provided |
| 5UCF Solution NMR-derived model of the minor species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1) Deposited 2016-12-22 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
373–427（55 aa）
Fragment：UNP residues 373-427
|
Mutation：Y3F/L5A/L7I/A34F/V39L | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 10 mM sodium phosphate；Pressure 1
NMR sample composition
200 uM [U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
200 uM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 100% D2O | 100% D2O
NMR sample composition
200 uM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6C9O Selenomethionine mutant (V29Sem) of protein GB1 examined by X-ray diffraction Deposited 2018-01-28 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Mutation：V29M Non-standard monomer：Yes (specific site not provided by mmCIF) | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；283.15 K；46% MPD, 20% IPA, 25 mM sodium acetate pH 4.5
|
Resolution 1.20 Å R-free 0.179 |
| 6C9O Selenomethionine mutant (V29Sem) of protein GB1 examined by X-ray diffraction Deposited 2018-01-28 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
304–357（54 aa）
|
Mutation：V29M Non-standard monomer：Yes (specific site not provided by mmCIF) | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；283.15 K；46% MPD, 20% IPA, 25 mM sodium acetate pH 4.5
|
Resolution 1.20 Å R-free 0.179 |
| 6CHE Selenomethionine mutant (A34Sem) of protein GB1 examined by X-ray diffraction Deposited 2018-02-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Mutation：A34Sem Non-standard monomer：Yes (specific site not provided by mmCIF) | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 IMD IMIDAZOLE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；283.15 K；49% MPD
20% IPA
25 mM sodium acetate pH 4.9
20 mg/ml protein in 25 mM sodium acetate buffer pH 5.5 and 2 mM TCEP
|
Resolution 1.10 Å R-free 0.147 |
| 6CPZ Selenomethionine mutant (I6Sem) of protein GB1 examined by X-ray diffraction Deposited 2018-03-14 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Mutation：I6Sem Non-standard monomer：Yes (specific site not provided by mmCIF) | MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 PO4 PHOSPHATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；283.15 K；48% MPD
20% IPA
25 mM sodium acetate buffer pH 4.7
20 mg/ml protein in 25 mM sodium acetate buffer pH 5.5 and 2 mM TCEP
|
Resolution 1.12 Å R-free 0.156 |
| 6CPZ Selenomethionine mutant (I6Sem) of protein GB1 examined by X-ray diffraction Deposited 2018-03-14 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
304–357（54 aa）
|
Mutation：I6Sem Non-standard monomer：Yes (specific site not provided by mmCIF) | MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 PO4 PHOSPHATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；283.15 K；48% MPD
20% IPA
25 mM sodium acetate buffer pH 4.7
20 mg/ml protein in 25 mM sodium acetate buffer pH 5.5 and 2 mM TCEP
|
Resolution 1.12 Å R-free 0.156 |
| 6CTE 77Se-NMR probes the protein environment of selenomethionine Deposited 2018-03-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Mutation：V39Sem Non-standard monomer：Yes (specific site not provided by mmCIF) | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 PO4 PHOSPHATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；283.15 K；49% MPD
20% IPA
25 mM sodium acetate pH 4.7
20 mg/ml protein concentration
25 mM sodium acetate pH 5.5
non-reducing
|
Resolution 1.20 Å R-free 0.175 |
| 6CTE 77Se-NMR probes the protein environment of selenomethionine Deposited 2018-03-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
304–357（54 aa）
|
Mutation：V39Sem Non-standard monomer：Yes (specific site not provided by mmCIF) | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 PO4 PHOSPHATE ION × 3 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 ACT ACETATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；283.15 K；49% MPD
20% IPA
25 mM sodium acetate pH 4.7
20 mg/ml protein concentration
25 mM sodium acetate pH 5.5
non-reducing
|
Resolution 1.20 Å R-free 0.175 |
| 6HKA The solution structure of the micelle-associated FATC domain of the human protein kinase ataxia telangiectasia mutated (ATM) Deposited 2018-09-06 | Different construct Different oligomeric state Different experimental conditions | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
100 mM sodium chloride, 50 mM TRIS, 150 mM [U-100% 2H] DPC, 0.4 mM [U-100% 13C; U-100% 15N] 13C-15N-hATMfatc, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
100 mM sodium chloride, 50 mM TRIS, 150 mM [U-100% 2H] DPC, 0.4 mM [U-10% 13C] 13C-hATMfatc, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
100 mM sodium chloride, 50 mM TRIS, 150 mM [U-100% 2H] DPC, 0.5 mM [U-100% 15N] 15N-hATMfatc, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 6HPJ Structure of human SRSF1 RRM1 bound to AACAAA RNA Deposited 2018-09-21 | Different construct Different oligomeric state Different experimental conditions | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: dimeric |
Chain B
304–357（54 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；313 K；Ionic strength (raw mmCIF value) 120；Pressure atmospheric
NMR sample composition
0.5 mM [U-99% 15N] SRSF1 RRM1, 0.5 mM RNA (5'-R(*AP*AP*CP*AP*AP*A)-3'), 100% D2O | 100% D2O
NMR sample composition
0.5 mM [U-99% 15N] SRSF1 RRM1, 0.5 mM NA RNA (5'-R(*AP*AP*CP*AP*AP*A)-3'), 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM [U-99% 13C; U-99% 15N] SRSF1 RRM1, 0.5 mM NA RNA (5'-R(*AP*AP*CP*AP*AP*A)-3'), 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6NJF Solution NMR Structure of DANCER3-F34A, a rigid and natively folded single mutant of the dynamic protein DANCER-3 Deposited 2019-01-03 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
373–427（55 aa）
Fragment：residues 373-427
|
Mutation：Y3F, V7I, V39L, V54I | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 10 mM sodium phosphate；Pressure 1
NMR sample composition
1.0 mM [U-98% 15N] protein (GB1), 10 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.0 mM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.0 mM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100% D2O | 100% D2O
|
Resolution not provided |
| 6NL6 Crystal structure of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, N35K, D36H, N37Q) Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
|
Mutation：T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, D36H, N35K, D36A, N37Q | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；70 mM acetic Acid pH 3.6, 30 mM acetic acid pH 5.8, 30% 2,4-methylpentanediol, 100 mM NaCl, and 20 mM zinc sulfate
|
Resolution 1.40 Å R-free 0.214 |
| 6NL6 Crystal structure of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, N35K, D36H, N37Q) Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
303–357（55 aa）
|
Mutation：T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, D36H, N35K, D36A, N37Q | ZN ZINC ION × 3 CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；70 mM acetic Acid pH 3.6, 30 mM acetic acid pH 5.8, 30% 2,4-methylpentanediol, 100 mM NaCl, and 20 mM zinc sulfate
|
Resolution 1.40 Å R-free 0.214 |
| 6NL6 Crystal structure of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, N35K, D36H, N37Q) Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
303–357（55 aa）
|
Mutation：T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, D36H, N35K, D36A, N37Q | ZN ZINC ION × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；70 mM acetic Acid pH 3.6, 30 mM acetic acid pH 5.8, 30% 2,4-methylpentanediol, 100 mM NaCl, and 20 mM zinc sulfate
|
Resolution 1.40 Å R-free 0.214 |
| 6NL6 Crystal structure of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, N35K, D36H, N37Q) Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
303–357（55 aa）
|
Mutation：T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, D36H, N35K, D36A, N37Q | ZN ZINC ION × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；70 mM acetic Acid pH 3.6, 30 mM acetic acid pH 5.8, 30% 2,4-methylpentanediol, 100 mM NaCl, and 20 mM zinc sulfate
|
Resolution 1.40 Å R-free 0.214 |
| 6NL7 Crystal structure of B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q) Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
|
Mutation：T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q | ZN ZINC ION × 2 ACT ACETATE ION × 2 NA SODIUM ION × 2 CL CHLORIDE ION × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；80 mM acetic acid pH 3.6, 20 mM acetic acid pH 5.8, 30% 2,4-methylpentanediol, 200 mM NaCl and 20 mM zinc sulfate
|
Resolution 1.40 Å R-free 0.158 |
| 6NL7 Crystal structure of B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q) Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
303–357（55 aa）
|
Mutation：T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q | ZN ZINC ION × 2 ACT ACETATE ION × 3 NA SODIUM ION × 1 PO4 PHOSPHATE ION × 1 DPO DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；80 mM acetic acid pH 3.6, 20 mM acetic acid pH 5.8, 30% 2,4-methylpentanediol, 200 mM NaCl and 20 mM zinc sulfate
|
Resolution 1.40 Å R-free 0.158 |
| 6NL7 Crystal structure of B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q) Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
303–357（55 aa）
|
Mutation：T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q | ACT ACETATE ION × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；80 mM acetic acid pH 3.6, 20 mM acetic acid pH 5.8, 30% 2,4-methylpentanediol, 200 mM NaCl and 20 mM zinc sulfate
|
Resolution 1.40 Å R-free 0.158 |
| 6NL7 Crystal structure of B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q) Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
303–357（55 aa）
|
Mutation：T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q | ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；80 mM acetic acid pH 3.6, 20 mM acetic acid pH 5.8, 30% 2,4-methylpentanediol, 200 mM NaCl and 20 mM zinc sulfate
|
Resolution 1.40 Å R-free 0.158 |
| 6NL8 Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, T16L, V29H, Y33H, N37L)-zinc Deposited 2019-01-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
303–357（55 aa）
|
Mutation：L12H, T16L, V29H, Y33H, N37L | ZN ZINC ION × 4 CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；18.5% PEG 400 0.1M HEPES pH 7.5
50 mM MgCl2, 5mM zinc sulfate
|
Resolution 1.50 Å R-free 0.163 |
| 6NL9 Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, T16L, V29H, Y33H, N37L)-apo Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
|
Mutation：L12H, T16L, V29H, Y33H, N37L | MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；30% PEG 4000 0.1M HEPES pH 7.5
200 mM MgCl2
|
Resolution 1.70 Å R-free 0.224 |
| 6NL9 Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, T16L, V29H, Y33H, N37L)-apo Deposited 2019-01-08 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
303–357（55 aa）
|
Mutation：L12H, T16L, V29H, Y33H, N37L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；30% PEG 4000 0.1M HEPES pH 7.5
200 mM MgCl2
|
Resolution 1.70 Å R-free 0.224 |
| 6NL9 Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, T16L, V29H, Y33H, N37L)-apo Deposited 2019-01-08 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
303–357（55 aa）
|
Mutation：L12H, T16L, V29H, Y33H, N37L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；30% PEG 4000 0.1M HEPES pH 7.5
200 mM MgCl2
|
Resolution 1.70 Å R-free 0.224 |
| 6NL9 Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, T16L, V29H, Y33H, N37L)-apo Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
303–357（55 aa）
|
Mutation：L12H, T16L, V29H, Y33H, N37L | NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；30% PEG 4000 0.1M HEPES pH 7.5
200 mM MgCl2
|
Resolution 1.70 Å R-free 0.224 |
| 6NLA Crystal structure of de novo designed metal-controlled dimer of B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)-zinc Deposited 2019-01-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
303–357（55 aa）
|
Mutation：L12H, E15V, T16L, T18I, V29H, Y33H, N37L | ZN ZINC ION × 4 CL CHLORIDE ION × 6 GOL GLYCEROL × 2 NA SODIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；4M NaCl 0.1M HEPES pH 7.5
50mM MgCl2, 5mM zinc sulfate
|
Resolution 1.34 Å R-free 0.128 |
| 6NLB Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)-apo Deposited 2019-01-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
|
Mutation：L12H, E15V, T16L, T18I, V29H, Y33H, N37L | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；31% PEG 4,000 0.1M Tris pH 8.5
200mM MgCl2
|
Resolution 2.30 Å R-free 0.258 |
| 6NLB Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)-apo Deposited 2019-01-08 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
303–357（55 aa）
|
Mutation：L12H, E15V, T16L, T18I, V29H, Y33H, N37L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；31% PEG 4,000 0.1M Tris pH 8.5
200mM MgCl2
|
Resolution 2.30 Å R-free 0.258 |
| 6NLB Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)-apo Deposited 2019-01-08 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
303–357（55 aa）
|
Mutation：L12H, E15V, T16L, T18I, V29H, Y33H, N37L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；31% PEG 4,000 0.1M Tris pH 8.5
200mM MgCl2
|
Resolution 2.30 Å R-free 0.258 |
| 6NLB Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)-apo Deposited 2019-01-08 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
303–357（55 aa）
|
Mutation：L12H, E15V, T16L, T18I, V29H, Y33H, N37L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；31% PEG 4,000 0.1M Tris pH 8.5
200mM MgCl2
|
Resolution 2.30 Å R-free 0.258 |
| 6O41 Crystal structure of the unbound PGZL1 germline Fab fragment (PGZL1_gVmDmJ) Deposited 2019-02-27 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain M
438–497（60 aa）
|
Not recorded | GOL GLYCEROL × 7 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；20% PEG3350, 0.2 M lithium citrate
|
Resolution 2.46 Å R-free 0.219 |
| 6O41 Crystal structure of the unbound PGZL1 germline Fab fragment (PGZL1_gVmDmJ) Deposited 2019-02-27 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain O
438–497（60 aa）
|
Not recorded | GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；20% PEG3350, 0.2 M lithium citrate
|
Resolution 2.46 Å R-free 0.219 |
| 6O41 Crystal structure of the unbound PGZL1 germline Fab fragment (PGZL1_gVmDmJ) Deposited 2019-02-27 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain N
438–497（60 aa）
|
Not recorded | GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；20% PEG3350, 0.2 M lithium citrate
|
Resolution 2.46 Å R-free 0.219 |
| 6OC7 HMP42 Fab in complex with Protein G Deposited 2019-03-22 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
438–497（60 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；17% PEG 4000, 15% glycerol, 8.5% 2-propanol and 85 mM HEPES pH 7.5
|
Resolution 1.30 Å R-free 0.184 |
| 6UUH Crystal structure of broad and potent HIV-1 neutralizing antibody 438-B11 Deposited 2019-10-30 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain E
438–497（60 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；0.085M sodium acetate (pH=4.0), 0.17M ammonium acetate, 5% %(v/v) glycerol, 27.882 %(w/v) PEG4000, 15% glycerol
|
Resolution 2.70 Å R-free 0.260 |
| 6UUH Crystal structure of broad and potent HIV-1 neutralizing antibody 438-B11 Deposited 2019-10-30 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain F
438–497（60 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；0.085M sodium acetate (pH=4.0), 0.17M ammonium acetate, 5% %(v/v) glycerol, 27.882 %(w/v) PEG4000, 15% glycerol
|
Resolution 2.70 Å R-free 0.260 |
| 6UYG Structure of Hepatitis C Virus Envelope Glycoprotein E2c3 core from genotype 6a bound to broadly neutralizing antibody AR3A and non neutralizing antibody E1 Deposited 2019-11-13 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain G
438–497（60 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.2M magnesium chloride, 10% (w/v) PEG 3000, 15% ethylene glycol, 0.1M Na-cacodylate, pH=6.5
|
Resolution 3.38 Å R-free 0.305 |
| 6W00 Crystal structure of Fab239 in complex with NPNA2 peptide from circumsporozoite protein Deposited 2020-02-28 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain G
440–497（58 aa）
Fragment：domain III (UNP residues 438-497)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；0.2 M sodium chloride, 20% w/v PEG3350
|
Resolution 1.85 Å R-free 0.206 |
| 6WFW Crystal structure of Fab364 in complex with NPNA2 peptide from circumsporozoite protein Deposited 2020-04-04 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain G
439–497（59 aa）
Fragment：domain III (UNP residues 438-497)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277.15 K；20% PEG8000, 0.05 M potassium phosphate dibasic
|
Resolution 2.09 Å R-free 0.257 |
| 7DA8 X-ray structure of a GB1:T2Q/D46K mutant Deposited 2020-10-15 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
|
Mutation：T303Q, D347K | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；MES monohydrate pH 6.5 (buffer system 1), 40% v/v PEG 500* MME, 20 % w/v PEG 20000 (Precipitant mix1) (Morpheus Screen ID 25).
|
Resolution 2.40 Å R-free 0.305 |
| 8DIJ NMR Structure of Streptococcal Protein GB1 Backbone Modified Variant: beta-ACPC24, beta-3-Lys28, beta-3-Lys31, beta-ACPC35 Deposited 2022-06-29 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
302–357（56 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 40；Pressure 1
NMR sample composition
0.65 mM Streptococcal Protein GB1 Backbone Modified Variant: beta-ACPC24, beta-3-Lys28, beta-3-Lys31, beta-ACPC35, 20 mM sodium phosphate, 0.1 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 8TFR Apo Fab from C10-S66K antibody Deposited 2023-07-11 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
438–497（60 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；20% PEG 3350, 0.2 M Na3-citrate, pH 8.2
|
Resolution 2.99 Å R-free 0.262 |
| 8UM7 Site-specific Aspartic Acid Dehydration and Isomerization in Streptococcal Protein GB1: Wild-type Protein Deposited 2023-10-17 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 41；Pressure 1
NMR sample composition
0.12 mM B1 Domain of Streptococcal Protein G, 20 mM sodium phosphate, 0.1 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 8UM9 Site-specific Aspartic Acid Dehydration and Isomerization in Streptococcal Protein GB1: D-Asp40 Variant Deposited 2023-10-17 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 41；Pressure 1
NMR sample composition
0.12 mM B1 Domain of Streptococcal Protein G, D-Asp40 Variant, 20 mM sodium phosphate, 0.1 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 8UMA Site-specific Aspartic Acid Dehydration and Isomerization in Streptococcal Protein GB1: D-isoAsp40 Variant Deposited 2023-10-17 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 41；Pressure 1
NMR sample composition
0.17 mM B1 Domain of Streptococcal Protein G, D-isoAsp40 Variant, 20 mM sodium phosphate, 0.1 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 8UMB Site-specific Aspartic Acid Dehydration and Isomerization in Streptococcal Protein GB1: L-Aspartyl Succinimide 40-41 Variant Deposited 2023-10-17 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 41；Pressure 1
NMR sample composition
0.07 mM B1 Domain of Streptococcal Protein G, L-Aspartyl Succinimide 40-41 Variant, 20 mM sodium phosphate, 0.1 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 8UMS Site-specific Aspartic Acid Dehydration and Isomerization in Streptococcal Protein GB1: L-isoAsp40 Variant Deposited 2023-10-18 | Different construct Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
304–357（54 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 41；Pressure 1
NMR sample composition
0.18 mM B1 Domain of Streptococcal Protein G, L-isoAsp40 Variant, 20 mM sodium phosphate, 0.1 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 8WCJ Crystal structure of GB3 penta mutation L5V/K10H/T16S/K19E/Y33I Deposited 2023-09-12 | Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
444–497（54 aa）
|
Mutation：L446V, K451H, T457S, K460E, Y474I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；60% v/v Tacsimate pH 7.0
|
Resolution 1.55 Å R-free 0.233 |
| 9AWE The crystal structure of an engineered Protein GF with Human Kappa Fab Deposited 2024-03-05 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
368–430（63 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M Sodium Cacodylate pH 6.0, 0.1M Calcium acetate hydrate, 10% PEG 8000
|
Resolution 2.80 Å R-free 0.293 |
| 9BDE Middle Region of Apolipoprotein B 100 bound to Low Density Lipoprotein Receptor Deposited 2024-04-11 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
440–497（58 aa）
Fragment：;residues 27-384 (Uniprot numbering),residues 278-327 (Uniprot numbering),103-151 (Uniprot numbering),residues 440-497 (Uniprot numbering)
;
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 CA CALCIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.18 Å |
| 9I2I X-ray structure of the B1 domain of streptococcal protein G triple mutant T2Q, N8D, and N37D (GB1-QDD). Deposited 2025-01-20 | Different construct Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；278 K；50% MPD, 25% isopropyl alcohol,
|
Resolution 1.08 Å R-free 0.210 |
| 9I2I X-ray structure of the B1 domain of streptococcal protein G triple mutant T2Q, N8D, and N37D (GB1-QDD). Deposited 2025-01-20 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
303–357（55 aa）
|
Not recorded | NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；278 K；50% MPD, 25% isopropyl alcohol,
|
Resolution 1.08 Å R-free 0.210 |
| 9QBJ Legobody dimer Deposited 2025-03-03 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain I
440–496（57 aa）
Chain J
440–497（58 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9T8Z Room temperature X-ray structure of the B1 domain of streptococcal protein G triple mutant T2Q, N8D, and N37D (GB1-QDD). Deposited 2025-11-13 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
303–357（55 aa）
|
Not recorded | NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；278 K；50 % 2-methyl-2,4-pentanediol (MPD), 25 % isopropanol
|
Resolution 1.69 Å R-free 0.197 |
| 9T8Z Room temperature X-ray structure of the B1 domain of streptococcal protein G triple mutant T2Q, N8D, and N37D (GB1-QDD). Deposited 2025-11-13 | Different construct Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
303–357（55 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；278 K；50 % 2-methyl-2,4-pentanediol (MPD), 25 % isopropanol
|
Resolution 1.69 Å R-free 0.197 |
76 other PDB entries and 116 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | SPG2_STRSG |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–64； UniProt 367–430 |
