2iwh

Structure of yeast Elongation Factor 3 in complex with ADPNP

Method: X-RAY DIFFRACTION Dmax: 121.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 2iwh

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2iwh
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2iwh
沉积日期 deposition_date2006-06-30
结构标题 titleStructure of yeast Elongation Factor 3 in complex with ADPNP
关键词 keywords;PROTEIN BIOSYNTHESIS, TRANSLATION, PHOSPHORYLATION, ELONGATION FACTOR, NUCLEOTIDE-BINDING, RNA-BINDING, ACETYLATION, ATP-BINDING, RRNA-BINDING ;; TRANSLATION
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier38.67
回转半径 Rg (电子) rg_electron37.80
零角强度 I(0) i0730026000.00
分子量 molecular_weight218810.0 kDa
排除体积 excluded_volume273250 ų
包络体积 envelope_volume356380 ų
水化壳体积 shell_volume75001 ų
包络直径 envelope_diameter126.2
壳层 Rg shell_rg46.56
包络 Rg envelope_rg37.09
形状 Rg shape_rg37.80
总 Rg total_rg38.30
总原子数 total_atoms15348
残基数 n_residues1954
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax121.8
Rg (实空间) rg_real38.42
Rg 误差 (实空间) rg_real_error0.63
I(0) (实空间) i0_real7.3000e+08
I(0) 误差 (实空间) i0_real_error1.2170e+07
Rg (倒空间) rg_reciprocal38.58
I(0) (倒空间) i0_reciprocal730100000.0000
解质量估计 total_estimate0.8870
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary50.5
偏度 Skewness skewness0.170
峰度 Kurtosis kurtosis-0.409
角度范围 angular_range— – 0.2050 −1
当前正则化参数 α current_alpha0.0001
最高正则化参数 α highest_alpha225700000.0000
实空间数据点数 n_real_points42
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.887; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.972; Smooth: 0.894

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (3)

6. 折叠分类 (SCOP + CATH) 10 domains

CATH v4.4 (10 domains)

结构域编号 domain_id2iwhA01
类 Class class1 — Mainly Alpha
架构 Architecture architecture25 — Alpha Horseshoe
拓扑 Topology topology10 — Leucine-rich Repeat Variant
同源超家族 H-superfamily homologous superfamily10 — Leucine-rich Repeat Variant
结构域编号 domain_id2iwhA02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1390 — PWI domain
同源超家族 H-superfamily homologous superfamily20
结构域编号 domain_id2iwhA03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id2iwhA04
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id2iwhA05
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily990
结构域编号 domain_id2iwhB01
类 Class class1 — Mainly Alpha
架构 Architecture architecture25 — Alpha Horseshoe
拓扑 Topology topology10 — Leucine-rich Repeat Variant
同源超家族 H-superfamily homologous superfamily10 — Leucine-rich Repeat Variant
结构域编号 domain_id2iwhB02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1390 — PWI domain
同源超家族 H-superfamily homologous superfamily20
结构域编号 domain_id2iwhB03
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id2iwhB04
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id2iwhB05
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily990

7. 引用文献 (1)

8. 文件与曲线 (10)