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1PDO
PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM
Deposited 1996-03-08
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Different construct
Different oligomeric state
Different experimental method
Different experimental conditions
Different structure-quality metrics
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Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
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Chain A
1–132（132 aa）
Fragment：IIA ==MAN== DOMAIN, RESIDUES 2 - 133, OF THE IIAB ==MAN== SUBUNIT PLUS PHE-ALA-GLY AT THE CARBOXY TERMINUS
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Not recorded
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No recorded non-water small molecule
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X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.8；pH 6.8
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Resolution 1.70 Å
R-free 0.244
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1VRC
Complex of enzyme IIAmannose and the histidine-containing phosphocarrier protein HPr from escherichia coli nmr, restrained regularized mean structure
Deposited 2005-02-21
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Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
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Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
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Chain A
1–132（132 aa）
Fragment：EIIA DOMAIN
Chain B
1–132（132 aa）
Fragment：EIIA DOMAIN
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Not recorded
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PO3 PHOSPHITE ION × 2
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SOLUTION NMR
NMR measurement conditions
pH 6.5；308 K；Ionic strength (raw mmCIF value) 40 mM SODIUM PHOSPHATE
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Resolution not provided
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1VSQ
Solution NMR structure of the productive complex between IIAMannose and IIBMannose of the mannose transporter of the E. coli phosphotransferase system
Deposited 2008-01-10
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Different construct
Different mutation/modification
Different oligomeric state
Different experimental conditions
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Assembly 1
Protein homooligomer
Homooligomer；Protein × 3
PDB declaration: trimeric
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Chain A
2–134（133 aa）
Chain B
2–134（133 aa）
Chain C
159–323（165 aa）
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Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
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No recorded non-water small molecule
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SOLUTION NMR
NMR measurement conditions
pH 6.5；303 K；Ionic strength (raw mmCIF value) 20 mM phosphate；Pressure ambient
NMR sample composition
0.5-1 mM IIAMan(H10E)+IIBMan, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5-1 mM IIAMan(H10E)+IIBMan, 100% D2O | 100% D2O
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Resolution not provided
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2JZN
Solution NMR structure of the productive complex between IIAMannose and IIBMannose of the mannose transporter of the E. coli phosphotransferase system
Deposited 2008-01-10
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Different construct
Different mutation/modification
Different oligomeric state
Different experimental conditions
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Assembly 1
Protein homooligomer
Homooligomer；Protein × 3
PDB declaration: trimeric
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Chain A
2–134（133 aa）
Chain B
2–134（133 aa）
Chain C
159–323（165 aa）
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Mutation：H10E
Mutation：H10E
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No recorded non-water small molecule
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SOLUTION NMR
NMR measurement conditions
pH 6.5；303 K；Ionic strength (raw mmCIF value) 20 mM phosphate；Pressure ambient
NMR sample composition
0.5-1 mM see details IIAMan(H10E)+IIBMan, 100% D2O | 100% D2O
NMR sample composition
0.5-1 mM see details IIAMan(H10E)+IIBMan, 90% H2O/10% D2O | 90% H2O/10% D2O
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Resolution not provided
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2JZO
Solution NMR structure of the non-productive complex between IIAMannose and IIBMannose of the mannose transporter of the E. coli phosphotransferase system
Deposited 2008-01-10
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Different construct
Different mutation/modification
Different oligomeric state
Different experimental conditions
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Assembly 1
Protein homooligomer
Homooligomer；Protein × 3
PDB declaration: trimeric
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Chain A
2–134（133 aa）
Fragment：PTS EIIB type-4 domain
Chain B
2–134（133 aa）
Fragment：PTS EIIB type-4 domain
Chain D
159–323（165 aa）
Fragment：PTS EIIA type-4 domain
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Not recorded
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No recorded non-water small molecule
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SOLUTION NMR
NMR measurement conditions
pH 6.5；303 K；Ionic strength (raw mmCIF value) 20 mM phosphate；Pressure ambient
NMR sample composition
0.5-1 mM IIAMan+IIBMan, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5-1 mM IIAMan+IIBMan, 100% D2O | 100% D2O
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Resolution not provided
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