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2mkc

Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex

Method: SOLUTION NMR
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1. Protein Identity and Related Structures Protein Identity & Related Structures

U2 snRNP component IST3

Saccharomyces cerevisiae

UniProt P40565

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 Pre-mRNA leakage protein 1 × 1 （Q07930） Pre-mRNA-splicing factor CWC26 × 1 （P46947） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2mkc 2my2 2my3 4uqt 5gm6 5lqw 5zwm 5zwo

8 assemblies

View Construct and Data Evidence
UniProt name IST3_YEAST
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 5–118； UniProt 25–138

Pre-mRNA leakage protein 1

Saccharomyces cerevisiae

UniProt Q07930

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 U2 snRNP component IST3 × 1 （P40565） Pre-mRNA-splicing factor CWC26 × 1 （P46947） Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2jkd 2jkd 3els 3elv 3elv

5 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers
3els

1 assemblies

Heteromeric or nucleic-acid complex
2mkc 2my3 5gm6 5lqw 5zwm 5zwo 7dco

7 assemblies

View Construct and Data Evidence
UniProt name PML1_YEAST
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 3–23； UniProt 22–42

Pre-mRNA-splicing factor CWC26

Saccharomyces cerevisiae

UniProt P46947

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 U2 snRNP component IST3 × 1 （P40565） Pre-mRNA leakage protein 1 × 1 （Q07930） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2mkc 2my2 4uqt 5gm6 5lqw 5zwm 5zwo

7 assemblies

View Construct and Data Evidence
UniProt name CWC26_YEAST
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 3–33； UniProt 215–245

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2mkc
Deposition date deposition_date2014-02-04
Structure title titleCooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex
Keywords keywordsspliceosome, Snu17p, Bud13p, Pml1p, heterotrimer, cooperativity, RES, splicing, RRM, PROTEIN BINDING, Ist3p; SPLICING
Experimental Method methodSOLUTION NMR
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2mkc__assembly_1__model_4

Assembly 1 · Model 4 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2mkc__assembly_1__model_4 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2mkc__assembly_1__model_4 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）17.86 Å
Rg (electron density)16.46 Å
Total Rg17.63 Å
Atom count2743
Residues174
Excluded volume24591 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2mkc__assembly_1__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 2 2mkc__assembly_1__model_2 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 3 2mkc__assembly_1__model_3 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 4 2mkc__assembly_1__model_4 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 5 2mkc__assembly_1__model_5 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 6 2mkc__assembly_1__model_6 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 7 2mkc__assembly_1__model_7 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 8 2mkc__assembly_1__model_8 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 9 2mkc__assembly_1__model_9 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 10 2mkc__assembly_1__model_10 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 11 2mkc__assembly_1__model_11 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 12 2mkc__assembly_1__model_12 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 13 2mkc__assembly_1__model_13 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 14 2mkc__assembly_1__model_14 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 15 2mkc__assembly_1__model_15 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 16 2mkc__assembly_1__model_16 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 17 2mkc__assembly_1__model_17 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 18 2mkc__assembly_1__model_18 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 19 2mkc__assembly_1__model_19 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 20 2mkc__assembly_1__model_20 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id2mkcA00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily330 — RRM (RNA recognition motif) domain
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7. Citations (1)