2n1f

Structure and assembly of the mouse ASC filament by combined NMR spectroscopy and cryo-electron microscopy

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Apoptosis-associated speck-like protein

Mus musculus

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 15	No other associated polymer	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ASC_MOUSE
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–89； UniProt 2–90 Author chain B； PDBConstruct 1–89； UniProt 2–90 Author chain C； PDBConstruct 1–89； UniProt 2–90 Author chain D； PDBConstruct 1–89； UniProt 2–90 Author chain E； PDBConstruct 1–89； UniProt 2–90 Author chain F； PDBConstruct 1–89； UniProt 2–90 Author chain G； PDBConstruct 1–89； UniProt 2–90 Author chain H； PDBConstruct 1–89； UniProt 2–90 Author chain I； PDBConstruct 1–89； UniProt 2–90 Author chain J； PDBConstruct 1–89； UniProt 2–90 Author chain K； PDBConstruct 1–89； UniProt 2–90 Author chain L； PDBConstruct 1–89； UniProt 2–90 Author chain M； PDBConstruct 1–89； UniProt 2–90 Author chain N； PDBConstruct 1–89； UniProt 2–90 Author chain O； PDBConstruct 1–89； UniProt 2–90

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2n1f
Deposition date deposition_date	2015-04-01
Structure title title	Structure and assembly of the mouse ASC filament by combined NMR spectroscopy and cryo-electron microscopy
Keywords keywords	;mouse ASC filament, ASC Apoptosis-associated speck like protein containing a CARD, PYRIN domain, inflammasomes, death domain, APOPTOSIS ;; APOPTOSIS

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2n1f__assembly_1__model_8

Assembly 1 · Model 8 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2n1f__assembly_1__model_8 | I(q)

100 plotted points; both axes use logarithmic scales.

2n1f__assembly_1__model_8 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	36.97 Å
Rg (electron density)	35.72 Å
Total Rg	36.31 Å
Atom count	10590
Residues	1335
Excluded volume	191020 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2n1f__assembly_1__model_1`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download
1	2	`2n1f__assembly_1__model_2`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download
1	3	`2n1f__assembly_1__model_3`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download
1	4	`2n1f__assembly_1__model_4`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download
1	5	`2n1f__assembly_1__model_5`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download
1	6	`2n1f__assembly_1__model_6`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download
1	7	`2n1f__assembly_1__model_7`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download
1	8	`2n1f__assembly_1__model_8`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download
1	9	`2n1f__assembly_1__model_9`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download
1	10	`2n1f__assembly_1__model_10`	pentadecameric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (1)

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6. Fold Classification (SCOP + CATH) 15 domains

CATH v4.4 (15 domains)

Domain ID domain_id	2n1fA00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fB00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fC00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fD00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fE00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fF00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fG00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fH00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fI00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fJ00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fK00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fL00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fM00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fN00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

Domain ID domain_id	2n1fO00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	533 — Death Domain, Fas
Homologous superfamily homologous superfamily	10 — Death Domain, Fas

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Resolution resolution	4.00 Å
Resolution测定Method resolution_method	layer line analysis
颗粒数 num_particles	—

Description description	Apoptosis-associated speck-like protein
Molecular weight formula_weight	10118.7 kDa
Source method src_method	man
Copy count entity_count	15

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H,I,J,K,L,M,N,O
Atom count atom_count	105900

Source organism organism	Mus musculus
Common name common_name	mouse
NCBI taxonomy ID ncbi_taxonomy_id	10090
Host organism host_organism	Escherichia coli BL21(DE3)

Structure and assembly of the mouse ASC filament by combined NMR spectroscopy and cryo-electron microscopy

1. Protein Identity and Related Structures Protein Identity & Related Structures

Apoptosis-associated speck-like protein

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2n1f__assembly_1__model_8

2n1f__assembly_1__model_8 | I(q)

2n1f__assembly_1__model_8 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

6. Fold Classification (SCOP + CATH) 15 domains

CATH v4.4 (15 domains)

7. Citations (1)

Structure and assembly of the mouse ASC filament by combined NMR spectroscopy and cryo-electron microscopy

1. Protein Identity and Related Structures Protein Identity & Related Structures

Apoptosis-associated speck-like protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2n1f__assembly_1__model_8

2n1f__assembly_1__model_8 | I(q)

2n1f__assembly_1__model_8 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

6. Fold Classification (SCOP + CATH) 15 domains

CATH v4.4 (15 domains)

7. Citations (1)