2o8k

NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element

Method: SOLUTION NMR
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1. Protein Identity and Related Structures Protein Identity & Related Structures

RNA polymerase sigma factor RpoN

Aquifex aeolicus

UniProt O66858

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Monomer Protein 1 DNA 2 5'-D(*TP*TP*TP*TP*GP*GP*CP*AP*CP*GP*TP*TP*TP*C)-3' × 1 5'-D(*GP*AP*AP*AP*CP*GP*TP*GP*CP*CP*AP*AP*AP*A)-3' × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name O66858_AQUAE
Isoform —
PDB entities 3
Chains and sequence ranges Author chain A; PDBConstruct 3–63; UniProt 338–398

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2o8k
Deposition date deposition_date2006-12-12
Structure title titleNMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element
Keywords keywordsPROTEIN-DNA COMPLEX, HELIX-TURN-HELIX, TRANSCRIPTION FACTOR, SIGMA-54, RNA POLYMERASE, TRANSCRIPTION-DNA COMPLEX; TRANSCRIPTION/DNA
Experimental Method methodSOLUTION NMR
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2o8k__assembly_1__model_20

Assembly 1 · Model 20 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2o8k__assembly_1__model_20 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2o8k__assembly_1__model_20 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)16.71 Å
Rg (electron density)15.80 Å
Total Rg16.68 Å
Atom count1969
Residues91
Excluded volume17736 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2o8k__assembly_1__model_1 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 2 2o8k__assembly_1__model_2 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 3 2o8k__assembly_1__model_3 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 4 2o8k__assembly_1__model_4 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 5 2o8k__assembly_1__model_5 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 6 2o8k__assembly_1__model_6 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 7 2o8k__assembly_1__model_7 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 8 2o8k__assembly_1__model_8 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 9 2o8k__assembly_1__model_9 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 10 2o8k__assembly_1__model_10 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 11 2o8k__assembly_1__model_11 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 12 2o8k__assembly_1__model_12 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 13 2o8k__assembly_1__model_13 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 14 2o8k__assembly_1__model_14 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 15 2o8k__assembly_1__model_15 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 16 2o8k__assembly_1__model_16 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 17 2o8k__assembly_1__model_17 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 18 2o8k__assembly_1__model_18 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 19 2o8k__assembly_1__model_19 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
1 20 2o8k__assembly_1__model_20 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id2o8kA01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily60 — Homeodomain-like
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7. Citations (1)