2qwa

THE X-RAY STRUCTURE OF A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE

Method: X-RAY DIFFRACTION D_max: 77.6 Å Quality: GOOD

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

NEURAMINIDASE

物种未注明

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	其他组合同源多聚体蛋白 × 4 其他聚合物 8 PDB 声明：tetrameric（4）与蛋白拷贝数一致	链 A； UniProt 83–470	片段：RESIDUES 82 - 468 突变：R292K (N2-TOKYO/67 NUMBERING)	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 4 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8	X-RAY DIFFRACTION X-ray结晶条件：pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 1.70 Å

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 2QWA	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1A14 COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 5 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE 提交 1997-12-21	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 N 83–470（388 aa）片段：RESIDUES 82 - 468	未记录	MAN alpha-D-mannopyranose × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 6.6；NC10 SCFV(5) AND N9 NA WERE MIXED TOGETHER (SCFV(5) IN FOUR-FOLD MOLAR EXCESS) WITH AND EQUAL VOLUME OF POTASSIUM PHOSPHATE BUFFER 1.7M PH6.6. THE DROP WAS EQUILIBRATED BY VAPOR DIFFUSION AGAINST PHOSPHATE BUFFER 1.3M PH6.8., vapor diffusion	分辨率 2.50 Å R-free 0.270
1BJI THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH THE GLAXO 6-CARBOXAMIDE SIALIC ACID ANALOGUE GR217029 提交 1997-12-03	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	未记录	MAN alpha-D-mannopyranose × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4 DPC 5-ACETYLAMINO-4-AMINO-6-(PHENETHYL-PROPYL-CARBAMOYL)-5,6-DIHYDRO-4H-PYRAN-2-CARBOXYLIC ACID × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 7；1.9M PHOSPHATE(PH 5.9) THE GLAXO 6-CARBOXAMIDE COMPOUND GR217029 WAS SOAKED IN TERN N9 NEURAMININDASE CRYSTALS AT 5 MM FOR 24HR. THIS EXPERIMENT WAS CARRIED OUT TO DETERMINE THE BINDING MODE OF THE 6-CARBOXAMIDE HYDROPHOBIC GROUP. A NEW HYDROPHOBIC POCKET WAS DISCOVERED EARLIER WITH A METHYL, ETHYL GROUP ATTACHED TO THE AMIDE OF TH3E SIX-CARBOXAMIDE. THIS COMPOUND HAS A MUCH LARGER HYDROPHOBIC GROUP ATTACHED., pH 7.0	分辨率 2.00 Å
1INY A SIALIC ACID DERIVED PHOSPHONATE ANALOG INHIBITS DIFFERENT STRAINS OF INFLUENZA VIRUS NEURAMINIDASE WITH DIFFERENT EFFICIENCIES 提交 1994-09-26	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4 EQP (1R)-4-acetamido-1,5-anhydro-2,4-dideoxy-1-phosphono-D-glycero-D-galacto-octitol × 4	X-RAY DIFFRACTION X-ray结晶条件 NATIVE CRYSTALS SOAKED IN 10MM EPANA SOLUTION, PH 7.2.	分辨率 2.40 Å
1L7F Crystal structure of influenza virus neuraminidase in complex with BCX-1812 提交 2002-03-15	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：integral membrane protein, membrane stalk cleaved by pronase releasing fully active residues 82-468	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8 BCZ 3-(1-ACETYLAMINO-2-ETHYL-BUTYL)-4-GUANIDINO-2-HYDROXY-CYCLOPENTANECARBOXYLIC ACID × 4 GOL GLYCEROL × 12	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 5.9；293 K；phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.80 Å R-free 0.187
1L7G Crystal structure of E119G mutant influenza virus neuraminidase in complex with BCX-1812 提交 2002-03-15	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：integral membrane protein, membrane stalk cleaved by pronase releasing fully active residues 82-468	突变：E119G	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8 BCZ 3-(1-ACETYLAMINO-2-ETHYL-BUTYL)-4-GUANIDINO-2-HYDROXY-CYCLOPENTANECARBOXYLIC ACID × 4 GOL GLYCEROL × 12	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 5.9；293 K；phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.85 Å R-free 0.168
1L7H Crystal structure of R292K mutant influenza virus neuraminidase in complex with BCX-1812 提交 2002-03-15	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：integral membrane protein, membrane stalk cleaved by pronase releasing fully active residues 82-468	突变：R292K	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 12 BCZ 3-(1-ACETYLAMINO-2-ETHYL-BUTYL)-4-GUANIDINO-2-HYDROXY-CYCLOPENTANECARBOXYLIC ACID × 4 GOL GLYCEROL × 12	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 5.9；293 K；phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.85 Å R-free 0.177
1MWE THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE 提交 1997-08-27	突变/修饰不同聚集状态不同配体/离子不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 12 SIA N-acetyl-alpha-neuraminic acid × 8 CA CALCIUM ION × 8	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 1.70 Å
1NCA REFINED CRYSTAL STRUCTURE OF THE INFLUENZA VIRUS N9 NEURAMINIDASE-NC41 FAB COMPLEX 提交 1992-01-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 N 82–470（389 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1NCA REFINED CRYSTAL STRUCTURE OF THE INFLUENZA VIRUS N9 NEURAMINIDASE-NC41 FAB COMPLEX 提交 1992-01-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合异源复合物；蛋白 × 20 PDB 声明：eicosameric	链 N 82–470（389 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 16 CA CALCIUM ION × 8	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1NCB CRYSTAL STRUCTURES OF TWO MUTANT NEURAMINIDASE-ANTIBODY COMPLEXES WITH AMINO ACID SUBSTITUTIONS IN THE INTERFACE 提交 1992-01-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 N 82–470（389 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1NCB CRYSTAL STRUCTURES OF TWO MUTANT NEURAMINIDASE-ANTIBODY COMPLEXES WITH AMINO ACID SUBSTITUTIONS IN THE INTERFACE 提交 1992-01-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合异源复合物；蛋白 × 20 PDB 声明：eicosameric	链 N 82–470（389 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 8	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1NCC CRYSTAL STRUCTURES OF TWO MUTANT NEURAMINIDASE-ANTIBODY COMPLEXES WITH AMINO ACID SUBSTITUTIONS IN THE INTERFACE 提交 1992-01-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 N 82–470（389 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1NMC COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 15 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE 提交 1997-12-21	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 N 83–470（388 aa）片段：RESIDUES 82 - 468	未记录	MAN alpha-D-mannopyranose × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 6.6；NC10 SCFV(15) AND N9 NA WERE MIXED TOGETHER (SCFV(15) IN SLIGHT MOLAR EXCESS) WITH AND EQUAL VOLUME OF POTASSIUM PHOSPHATE BUFFER 1.7M PH6.6. THE DROP WAS EQUILIBRATED BY VAPOUR DIFFUSION AGAINST PHOSPHATE BUFFER 1.3M PH6.8.	分辨率 2.50 Å R-free 0.260
1NMC COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 15 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE 提交 1997-12-21	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	未记录	MAN alpha-D-mannopyranose × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 6.6；NC10 SCFV(15) AND N9 NA WERE MIXED TOGETHER (SCFV(15) IN SLIGHT MOLAR EXCESS) WITH AND EQUAL VOLUME OF POTASSIUM PHOSPHATE BUFFER 1.7M PH6.6. THE DROP WAS EQUILIBRATED BY VAPOUR DIFFUSION AGAINST PHOSPHATE BUFFER 1.3M PH6.8.	分辨率 2.50 Å R-free 0.260
1NNA THREE-DIMENSIONAL STRUCTURE OF INFLUENZA A N9 NEURAMINIDASE AND ITS COMPLEX WITH THE INHIBITOR 2-DEOXY 2,3-DEHYDRO-N-ACETYL NEURAMINIC ACID 提交 1993-03-08	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 84–470（387 aa）	未记录	CA CALCIUM ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1NNB THREE-DIMENSIONAL STRUCTURE OF INFLUENZA A N9 NEURAMINIDASE AND ITS COMPLEX WITH THE INHIBITOR 2-DEOXY 2,3-DEHYDRO-N-ACETYL NEURAMINIC ACID 提交 1993-03-08	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 84–470（387 aa）	未记录	CA CALCIUM ION × 4 DAN 2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC ACID × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å
1NNC INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) COMPLEXED WITH 4-GUANIDINO-NEU5AC2EN INHIBITOR 提交 1995-03-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	MAN alpha-D-mannopyranose × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4 ZMR ZANAMIVIR × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 1.80 Å
1XOE N9 Tern influenza neuraminidase complexed with (2R,4R,5R)-5-(1-Acetylamino-3-methyl-butyl-pyrrolidine-2, 4-dicarobyxylic acid 4-methyl esterdase complexed with 提交 2004-10-06	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 84–470（387 aa）片段：N9 tern Influenza Neuraminidase	未记录	MAN alpha-D-mannopyranose × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABX 5-[1-(ACETYLAMINO)-3-METHYLBUTYL]-4-(METHOXYCARBONYL)PROLINE × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.20 Å R-free 0.315
1XOE N9 Tern influenza neuraminidase complexed with (2R,4R,5R)-5-(1-Acetylamino-3-methyl-butyl-pyrrolidine-2, 4-dicarobyxylic acid 4-methyl esterdase complexed with 提交 2004-10-06	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 84–470（387 aa）片段：N9 tern Influenza Neuraminidase	未记录	MAN alpha-D-mannopyranose × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ABX 5-[1-(ACETYLAMINO)-3-METHYLBUTYL]-4-(METHOXYCARBONYL)PROLINE × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.20 Å R-free 0.315
1XOG N9 Tern Influenza neuraminidase complexed with a 2,5-Disubstituted tetrahydrofuran-5-carboxylic acid 提交 2004-10-06	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 84–470（387 aa）片段：N9 Tern neuraminidas	未记录	MAN alpha-D-mannopyranose × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ABW 5-[1-(ACETYLAMINO)-3-METHYLBUTYL]-2,5-ANHYDRO-3,4-DIDEOXY-4-(METHOXYCARBONYL)PENTONIC ACID × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å R-free 0.297
2C4A Structure of Neuraminidase Subtype N9 Complexed with 30 MM Sialic Acid (NANA, NEU5AC), Crystal Soaked for 3 Hours at 291 K. 提交 2005-10-17	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 83-470	未记录	SIA N-acetyl-alpha-neuraminic acid × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 6.8；PROTEIN WAS CRYSTALLISED FROM 1.9M POTASSIUM PHOSPHATE PH 6.8, THEN SOAKED FOR 3 HOURS IN 30 MM NEU5AC	分辨率 2.15 Å R-free 0.205
2C4L Structure of Neuraminidase Subtype N9 Complexed with 30 MM Sialic Acid (NANA, NEU5AC), Crystal Soaked for 24 Hours at 291 K and Finally Backsoaked for 30 Min in a Cryoprotectant Solution which did not contain NEU5AC 提交 2005-10-20	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 83-470	未记录	SIA N-acetyl-alpha-neuraminic acid × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 20 BMA beta-D-mannopyranose × 20 CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 6.8；PROTEIN WAS CRYSTALLISED FROM 1.9M POTASSIUM PHOSPHATE PH 6.8, THEN SOAKED FOR 24 HOURS IN 30 MM NEU5AC AND FINALLY BACKSOAKED FOR 30 MIN IN A CRYOPROTECTANT SOLUTION WHICH DID NOT CONTAIN NEU5AC	分辨率 2.15 Å R-free 0.196
2QWB THE X-RAY STRUCTURE OF A COMPLEX OF SIALIC ACID AND A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	突变：R292K (N2-TOKYO/67 NUMBERING)	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 SIA N-acetyl-alpha-neuraminic acid × 8 CA CALCIUM ION × 8	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 2.00 Å
2QWC THE X-RAY STRUCTURE OF A COMPLEX OF NEU5AC2EN AND A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	突变：R292K (N2-TOKYO/67 NUMBERING)	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8 DAN 2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC ACID × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 1.60 Å
2QWD THE X-RAY STRUCTURE OF A COMPLEX OF 4-AMINO-NEU5AC2EN AND A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	突变：R292K (N2-TOKYO/67 NUMBERING)	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8 4AM 4-AMINO-2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC ACID × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 2.00 Å
2QWE THE X-RAY STRUCTURE OF A COMPLEX OF 4-GUANIDINO-NEU5AC2EN AND A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	突变：R292K (N2-TOKYO/67 NUMBERING)	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8 ZMR ZANAMIVIR × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 2.00 Å
2QWF THE X-RAY STRUCTURE OF A COMPLEX OF N-ACETYL-4-GUANIDINO-6-METHYL(PROPYL)CARBOXAMIDE-4,5-DIHYDRO-2H-PYRAN-2-CARBOXYLIC ACID AND A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	突变：R292K (N2-TOKYO/67 NUMBERING)	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8 G20 5-N-acetyl-4-guanidino-6-methyl(propyl) carboxamide-4,5-dihydro-2H-pyran-2-carboxylic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 1.90 Å
2QWG THE X-RAY STRUCTURE OF A COMPLEX OF 5-N-ACETYL-4-AMINO-6-DIETHYLCARBOXAMIDE-4,5-DIHYDRO-2H-PYRAN-2-CARBOXYLIC ACID AND A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	突变：R292K (N2-TOKYO/67 NUMBERING)	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8 G28 5-N-acetyl-4-amino-6-diethyl carboxamide-4,5-dihydro-2H-pyran-2-carboxylic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 1.80 Å
2QWH THE X-RAY STRUCTURE OF A COMPLEX OF 5-N-ACETYL-5-AMINO-3-(1-ETHYLPROPOXY)-1-CYCLOHEXENE-1-CARBOXYLIC ACID (GS4071) AND A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	突变：R292K (N2-TOKYO/67 NUMBERING)	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 G39 (3R,4R,5S)-4-(acetylamino)-5-amino-3-(pentan-3-yloxy)cyclohex-1-ene-1-carboxylic acid × 4 CA CALCIUM ION × 8	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 1.80 Å
2QWI THE X-RAY STRUCTURE OF A COMPLEX OF N-ACETYL-4-GUANIDINO-6-METHYL(PROPYL)CARBOXAMIDE-4,5-DIHYDRO-2H-PYRAN-2-CARBOXYLIC ACID AND WILDTYPE TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	突变/修饰不同聚集状态不同配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	未记录	MAN alpha-D-mannopyranose × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4 G20 5-N-acetyl-4-guanidino-6-methyl(propyl) carboxamide-4,5-dihydro-2H-pyran-2-carboxylic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 2.00 Å
2QWJ THE X-RAY STRUCTURE OF A COMPLEX OF 5-N-ACETYL-4-AMINO-6-DIETHYLCARBOXAMIDE-4,5-DIHYDRO-2H-PYRAN-2-CARBOXYLIC ACID AND A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	突变/修饰不同配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8 G28 5-N-acetyl-4-amino-6-diethyl carboxamide-4,5-dihydro-2H-pyran-2-carboxylic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 2.00 Å
2QWK THE X-RAY STRUCTURE OF A COMPLEX OF 5-N-ACETYL-5-AMINO-3-(1-ETHYLPROPOXY)-1-CYCLOHEXENE-1-CARBOXYLIC ACID (GS4071) AND WILDTYPE TERN N9 INFLUENZA VIRUS NEURAMINIDASE 提交 1998-04-07	突变/修饰不同配体/离子不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：RESIDUES 82 - 468	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 G39 (3R,4R,5S)-4-(acetylamino)-5-amino-3-(pentan-3-yloxy)cyclohex-1-ene-1-carboxylic acid × 4 CA CALCIUM ION × 8	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；1.9M PHOSPHATE (PH 5.9)	分辨率 1.80 Å
3NN9 REFINED ATOMIC STRUCTURES OF N9 SUBTYPE INFLUENZA VIRUS NEURAMINIDASE AND ESCAPE MUTANTS 提交 1991-03-28	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.30 Å
3W09 Influenza virus neuraminidase subtype N9 (TERN) complexed with 2,3-dif guanidino-neu5ac2en inhibitor 提交 2012-10-25	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 GOL GLYCEROL × 28 ZGE 5-acetamido-2,6-anhydro-4-carbamimidamido-3,4,5-trideoxy-3-fluoro-D-glycero-D-galacto-non-2-enonic acid × 4 CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 5.9；298 K；1.9M ammonium phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.189
4DGR Influenza Subtype 9 Neuraminidase Benzoic Acid Inhibitor Complex 提交 2012-01-26	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 82–470（389 aa）片段：neuraminidase	未记录	BGC beta-D-glucopyranose × 12 GLC alpha-D-glucopyranose × 4 3LV 4-[2,2-bis(hydroxymethyl)-5-oxopyrrolidin-1-yl]-3-[(dipropylamino)methyl]benzoic acid × 4 PO4 PHOSPHATE ION × 4 CA CALCIUM ION × 8 K POTASSIUM ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.9；298 K；1.9 M phosphate buffer, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 1.55 Å R-free 0.152
4NN9 REFINED ATOMIC STRUCTURES OF N9 SUBTYPE INFLUENZA VIRUS NEURAMINIDASE AND ESCAPE MUTANTS 提交 1991-03-28	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.30 Å
4WEG influenza virus neuraminidase N9 in complex 2,3-difluorosialic acid 提交 2014-09-10	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：UNP residues 83-470	未记录	SFJ (2R,3R,4R,5R,6R)-5-acetamido-2,3-difluoro-4-hydroxy-6-[(1R,2R)-1,2,3-trihydroxypropyl]tetrahydro-2H-pyran-2-carboxylic acid × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 4 DF4 (3R,4R,5R,6R)-5-(acetylamino)-3-fluoro-4-hydroxy-6-[(1R,2R)-1,2,3-trihydroxypropyl]-3,4,5,6-tetrahydropyranium-2-carboxylate × 4 FSI 5-acetamido-3,5-dideoxy-3-fluoro-D-erythro-alpha-L-manno-non-2-ulopyranosonic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；1.7M potassium phosphate	分辨率 2.10 Å R-free 0.201
5NN9 REFINED ATOMIC STRUCTURES OF N9 SUBTYPE INFLUENZA VIRUS NEURAMINIDASE AND ESCAPE MUTANTS 提交 1991-03-28	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.30 Å
5W26 INFLUENZA VIRUS NEURAMINIDASE N9 IN COMPLEX WITH 4-DEOXYGENATED 2,3-DIFLUORO-N-ACETYLNEURAMINIC ACID 提交 2017-06-05	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：UNP RESIDUES 82-469	未记录	9SG (2R,3R,5R,6R)-5-acetamido-2,3-bis(fluoranyl)-6-[(1R,2R)-1,2,3-tris(oxidanyl)propyl]oxane-2-carboxylic acid × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 4 9WM (2~{R},3~{R},5~{R})-3-acetamido-5-fluoranyl-2-[(1~{R},2~{R})-1,2,3-tris(oxidanyl)propyl]-2,3,4,5-tetrahydropyran-1-ium-6-carboxylic acid × 4 9VP 5-acetamido-2,6-anhydro-3,4,5-trideoxy-3-fluoro-D-erythro-L-gluco-nononic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.7；298 K；1.7M POTASSIUM PHOSPHATE	分辨率 1.90 Å R-free 0.177
5W2U INFLUENZA VIRUS NEURAMINIDASE N9 IN COMPLEX WITH 7-DEOXYGENATED 2,3-DIFLUORO-N-ACETYLNEURAMINIC ACID 提交 2017-06-06	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：UNP RESIDUES 82-469	未记录	9SM (2R,3R,4R,5R,6S)-5-acetamido-6-[(2S)-2,3-bis(oxidanyl)propyl]-2,3-bis(fluoranyl)-4-oxidanyl-oxane-2-carboxylic acid × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 4 9S4 (2~{S},3~{R},4~{R},5~{R})-3-acetamido-2-[(2~{S})-2,3-bis(oxidanyl)propyl]-5-fluoranyl-4-oxidanyl-2,3,4,5-tetrahydropyran-1-ium-6-carboxylic acid × 4 9SJ 5-acetamido-2,6-anhydro-3,5,7-trideoxy-3-fluoro-D-threo-L-galacto-nononic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.7；289 K；1.7M POTASSIUM PHOSPHATE	分辨率 2.00 Å R-free 0.187
5W2W INFLUENZA VIRUS NEURAMINIDASE N9 IN COMPLEX WITH 8-DEOXYGENATED 2,3-DIFLUORO-N-ACETYLNEURAMINIC ACID 提交 2017-06-07	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：UNP RESIDUES 82-469	未记录	9SP (2R,3R,4R,5R,6R)-5-acetamido-6-[(1R)-1,3-bis(oxidanyl)propyl]-2,3-bis(fluoranyl)-4-oxidanyl-oxane-2-carboxylic acid × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 4 9SD (2~{R},3~{R},4~{R},5~{R})-3-acetamido-2-[(1~{R})-1,3-bis(oxidanyl)propyl]-5-fluoranyl-4-oxidanyl-2,3,4,5-tetrahydropyran-1-ium-6-carboxylic acid × 4 9S7 5-acetamido-2,6-anhydro-3,5,8-trideoxy-3-fluoro-D-threo-L-galacto-nononic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.7；298 K；1.7M POTASSIUM PHOSPHATE	分辨率 1.85 Å R-free 0.171
5W2Y INFLUENZA VIRUS NEURAMINIDASE N9 IN COMPLEX WITH 9-DEOXYGENATED 2,3-DIFLUORO-N-ACETYLNEURAMINIC ACID 提交 2017-06-07	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）片段：UNP RESIDUES 82-469	未记录	9T7 (2R,3R,4R,5R,6R)-5-acetamido-6-[(1R,2R)-1,2-bis(oxidanyl)propyl]-2,3-bis(fluoranyl)-4-oxidanyl-oxane-2-carboxylic acid × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 4 9TM (2~{R},3~{R},4~{R},5~{R})-3-acetamido-2-[(1~{R},2~{R})-1,2-bis(oxidanyl)propyl]-5-fluoranyl-4-oxidanyl-2,3,4,5-tetrahydropyran-1-ium-6-carboxylic acid × 4 9T1 5-acetamido-2,6-anhydro-3,5,9-trideoxy-3-fluoro-D-erythro-L-gluco-non-4-ulosonic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.7；298 K；1.7M POTASSIUM PHOSPHATE	分辨率 2.39 Å R-free 0.198
6CRD INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) with tetrabrachion (TB) domain stalk 提交 2018-03-16	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 信息不足同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）链 B 83–470（388 aa）链 E 83–470（388 aa）链 H 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；100 mM HEPES buffer at pH7.0 with 10% PEG6000	分辨率 2.57 Å R-free 0.306
6CRD INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) with tetrabrachion (TB) domain stalk 提交 2018-03-16	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 信息不足同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 C 83–470（388 aa）链 D 83–470（388 aa）链 F 83–470（388 aa）链 G 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；100 mM HEPES buffer at pH7.0 with 10% PEG6000	分辨率 2.57 Å R-free 0.306
6D3B INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) APO FORM 提交 2018-04-15	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 82–470（389 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 8 GOL GLYCEROL × 16	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；1.4 M KH2PO4 + 3.0 M K2HPO4 (2:1)	分辨率 1.40 Å R-free 0.170
6HCX Influenza Virus N9 Neuraminidase A complex with Zanamivir molecule (Tern). 提交 2018-08-16	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	CA CALCIUM ION × 8 GOL GLYCEROL × 12 ZMR ZANAMIVIR × 4 K POTASSIUM ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；N9 crystals were grown by hanging-drop vapour diffusion against a reservoir of 1.9M potassium phosphate, pH 6.8, starting with equal volumes of N9 NA (10-15 mg/ml in water) and potassium phosphate buffer 1.4M KH2PO4:3M K2HPO4 in ratio 8:4, pH 6.6 at 20 degrees celsius. Inhibitor complexes obtained by soaking N9 crystals in a solution of 1.4M potassium phosphate buffer, pH 6.8, containing 5mM of inhibitor for 3 hours at 18 degrees celsius. Soaked in glycerol cryo-buffer.	分辨率 1.30 Å R-free 0.125
6HEB Influenza A Virus N9 Neuraminidase complex with Oseltamivir (Tern). 提交 2018-08-20	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	CA CALCIUM ION × 8 GOL GLYCEROL × 24 G39 (3R,4R,5S)-4-(acetylamino)-5-amino-3-(pentan-3-yloxy)cyclohex-1-ene-1-carboxylic acid × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；N9 crystals were grown by hanging drop vapour diffusion against a reservoir of 1.9M potassium phosphate, pH 6.8, starting with equal volumes of N9 NA(10-15mg/ml in water) and potassium phosphate buffer 1.4M KH2PO4:3M K2HPO4 in ratio 8:4, pH 6.6 at 20 degrees celsius. Inhibitor complexeswere obtained by soaking N9 crystals in a solution of 1.4M potassium phosphate buffer, pH 6.8, containing 5 mM of inhibitor for 3 hours at 18 degrees celsius. Glycerol cryo-buffer also soaked in.	分辨率 1.75 Å R-free 0.142
6HFC Influenza A Virus N9 Neuraminidase Native (Tern). 提交 2018-08-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	CA CALCIUM ION × 8 GOL GLYCEROL × 28 K POTASSIUM ION × 8 CO2 CARBON DIOXIDE × 4 PO4 PHOSPHATE ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；N9 crystals were grown by-hanging drop vapour diffusion against a reservoir of 1.9M potassium phosphate, pH 6.8, starting with equal volumes of N9 NA solution (10 to 15 mg/ml in water) and potassium phosphate buffer 1.4M KH2PO4: 3M K2HPO4 in ratio 8:4, pH 6.6 at 20 degrees celsius. Large rhombic dodecahedral crystals grew within a few days.	分辨率 1.29 Å R-free 0.164
6HG0 Influenza A Virus N9 Neuraminidase complex with NANA (Tern/Australia). 提交 2018-08-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	CA CALCIUM ION × 8 GOL GLYCEROL × 16 PO4 PHOSPHATE ION × 4 SIA N-acetyl-alpha-neuraminic acid × 8 K POTASSIUM ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；N9 crystals were grown by hanging-drop vapour diffusion against a reservoir of 1.9M potassium phosphate, pH 6.8, starting with equal volumes of N9 NA (10-15 mg/ml in water) and potassium phosphate buffer 1.4M KH2PO4:3M K2HPO4 in ratio 8:4, pH 6.6 at 20 degrees celsius. Inhibitor complexes obtained by soaking N9 crystals in a solution of 1.4M potassium phosphate buffer, pH 6.8, containing 5mM of inhibitor for 3 hours at 4 degrees celsius. Soaked in glycerol cryo-buffer.	分辨率 1.30 Å R-free 0.120
6MCX INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) RECOMBINANT HEAD DOMAIN 提交 2018-09-02	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4 GOL GLYCEROL × 12	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；1.4 M KH2PO4 + 3.0 M K2HPO4 (2:1), PH 7.0, VAPOR DIFFUSION, HANGING DROP,	分辨率 2.30 Å R-free 0.206
6NN9 REFINED ATOMIC STRUCTURES OF N9 SUBTYPE INFLUENZA VIRUS NEURAMINIDASE AND ESCAPE MUTANTS 提交 1991-03-28	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.30 Å
7NN9 NATIVE INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) 提交 1995-03-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合同源多聚体；蛋白 × 4 PDB 声明：tetrameric	链 A 83–470（388 aa）	未记录	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.00 Å

共 47 个其他 PDB 条目、52 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	NRAM_IATRA
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–388； UniProt 83–470

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	2qwa
沉积日期 deposition_date	1998-04-07
结构标题 title	THE X-RAY STRUCTURE OF A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE
关键词 keywords	NEURAMINIDASE, INFLUENZA PROTEIN, DRUG RESISTANT VARIANT, GLYCOSYLATED PROTEIN, HYDROLASE; HYDROLASE
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	21.32 Å
回转半径 Rg (电子) rg_electron	20.48 Å
零角强度 I(0) i0	37653000.00
分子量 molecular_weight	45563.0 kDa
排除体积 excluded_volume	56085 Å³
包络体积 envelope_volume	64476 Å³
水化壳体积 shell_volume	25279 Å³
包络直径 envelope_diameter	78.4 Å
壳层 Rg shell_rg	28.08 Å
包络 Rg envelope_rg	21.41 Å
形状 Rg shape_rg	20.44 Å
总 Rg total_rg	21.45 Å
总原子数 total_atoms	3192
残基数 n_residues	387
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	77.6 Å
Rg (实空间) rg_real	21.23 Å
Rg 误差 (实空间) rg_real_error	0.55 Å
I(0) (实空间) i0_real	3.7650e+07
I(0) 误差 (实空间) i0_real_error	5.2810e+05
Rg (倒空间) rg_reciprocal	21.25 Å
I(0) (倒空间) i0_reciprocal	37650000.0000
解质量估计 total_estimate	0.8279
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	27.4 Å
偏度 Skewness skewness	0.306
峰度 Kurtosis kurtosis	-0.012
角度范围 angular_range	— – 0.3750 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	14490000.0000
实空间数据点数 n_real_points	69
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.596; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.976; Smooth: 0.993

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (6)

实体 1 Entity 1 polymer

描述 description	NEURAMINIDASE
分子量 formula_weight	43695.8 kDa
EC 编号 ec_number	3.2.1.18
突变 mutation	R292K (N2-TOKYO/67 NUMBERING)
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	3591

RDFNNLTKGLCTINSWHIYGKDNAVRIGEDSDVLVTREPYVSCDPDECRFYALSQGTTIRGKHSNGTIHDRSQYRALISWPLSSPPTVYNSRVECIGWSSTSCHDGKTRMSICISGPNNNASAVIWYNRRPVTEINTWARNILRTQESECVCHNGVCPVVFTDGSATGPAETRIYYFKEGKILKWEPLAGTAKHIEECSCYGERAEITCTCKDNWQGSNRPVIRIDPVAMTHTSQYICSPVLTDNPRPNDPTVGKCNDPYPGNNNNGVKGFSYLDGVNTWLGRTISIASRSGYEMLKVPNALTDDKSKPTQGQTIVLNTDWSGYSGSFMDYWAEGECYRACFYVELIRGRPKEDKVWWTSNSIVSMCSSTEFLGQWDWPDGAKIEYFL

实体 2 Entity 2 branched

描述 description	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
分子量 formula_weight	1235.1 kDa
来源方法 src_method	man
拷贝数 entity_count	1

实体 3 Entity 3 branched

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	424.4 kDa
来源方法 src_method	man
拷贝数 entity_count	1

实体 4 Entity 4 non-polymer

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	221.2 kDa
来源方法 src_method	man
拷贝数 entity_count	1

实体 5 Entity 5 non-polymer

描述 description	CALCIUM ION
分子量 formula_weight	40.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 6 Entity 6 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	399

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7. 折叠分类 (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

结构域编号 domain_id	d2qwaa_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.68 — 6-bladed beta-propeller
超家族 Superfamily superfamily	b.68.1 — Sialidases
家族 Family family	b.68.1.1 — Sialidases (neuraminidases)

CATH v4.4 (1 domains)

结构域编号 domain_id	2qwaA00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	120 — 6 Propeller
拓扑 Topology topology	10 — Neuraminidase
同源超家族 H-superfamily homologous superfamily	10 —

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8. 引用文献 (2)

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晶胞边长 a length_a	181.400 Å
晶胞边长 b length_b	181.400 Å
晶胞边长 c length_c	181.400 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	48

空间群 space_group	I 4 3 2
空间群编号 int_tables_number	211
晶系 crystal_system	—

分辨率 resolution	1.700 Å
最低分辨率 resolution_low	6.000 Å
R 因子 r_factor	0.1710
R_work r_work	0.1710
R_free r_free	—

fit	/app/data/saxs_computation/qw/2qwa/2qwa.fit
pddf	/app/data/pr_computation/qw/2qwa/2qwa.pr.out
profile	/app/data/saxs_computation/qw/2qwa/2qwa.dat