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2z71

Structure of truncated mutant CYS1GLY of penicillin V acylase from bacillus sphaericus co-crystallized with penicillin V

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Penicillin acylase

Bacillus sphaericus

UniProt P12256

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein homooligomer Homooligomer Protein 4 (2S,5R,6R)-3,3-DIMETHYL-7-OXO-6-(2-PHENOXYACETAMIDO)-4-THIA-1- AZABICYCLO(3.2.0)HEPTANE-2-CARBOXYLIC ACID × 4 water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer
2iwm 2pva 2quy 2quy 2z71 3mji 3pva 3pva

8 assemblies

Other homooligomers

None

Heteromeric or nucleic-acid complex

None

View Construct and Data Evidence
UniProt name PAC_BACSH
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–335； UniProt 4–338 Author chain C； PDBConstruct 1–335； UniProt 4–338

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2z71
Deposition date deposition_date2007-08-10
Structure title titleStructure of truncated mutant CYS1GLY of penicillin V acylase from bacillus sphaericus co-crystallized with penicillin V
Keywords keywordsZYMOGEN, HYDROLASE, PRECURSOR, PENICILLIN, AUTOPROTEOLYSIS, ANTIBIOTIC RESISTANCE, CATALYSIS, PENICILLIN V ACYLASE; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2z71__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2z71__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2z71__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）34.90 Å
Rg (electron density)34.06 Å
Total Rg34.74 Å
Atom count10418
Residues1322
Excluded volume186060 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2z71__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd2z71a_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.153 — Ntn hydrolase-like
Superfamily Superfamily superfamilyd.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family familyd.153.1.3 — Penicillin V acylase
Domain ID domain_idd2z71c_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.153 — Ntn hydrolase-like
Superfamily Superfamily superfamilyd.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family familyd.153.1.3 — Penicillin V acylase

CATH v4.4 (2 domains)

Domain ID domain_id2z71A00
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology60 — Penicillin V Acylase; Chain A
Homologous superfamily homologous superfamily10 — Penicillin V Acylase; Chain A
Domain ID domain_id2z71C00
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology60 — Penicillin V Acylase; Chain A
Homologous superfamily homologous superfamily10 — Penicillin V Acylase; Chain A
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7. Citations (4)