3b8o

Structure of WzzE- Bacterial Polysaccharide Co-polymerase

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Lipopolysaccharide biosynthesis protein wzzE

Escherichia coli

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 8	water × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	WZZE_ECO57
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–265； UniProt 55–319 Author chain B； PDBConstruct 1–265； UniProt 55–319 Author chain C； PDBConstruct 1–265； UniProt 55–319 Author chain D； PDBConstruct 1–265； UniProt 55–319 Author chain E； PDBConstruct 1–265； UniProt 55–319 Author chain F； PDBConstruct 1–265； UniProt 55–319 Author chain G； PDBConstruct 1–265； UniProt 55–319 Author chain H； PDBConstruct 1–265； UniProt 55–319

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	3b8o
Deposition date deposition_date	2007-11-01
Structure title title	Structure of WzzE- Bacterial Polysaccharide Co-polymerase
Keywords keywords	;WZZ, WzzE, Bacterial Polysaccharide Co-polymerase, Inner membrane, Lipopolysaccharide biosynthesis, Membrane, Transmembrane, MEMBRANE PROTEIN, BIOSYNTHETIC PROTEIN ;; BIOSYNTHETIC PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

3b8o__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

3b8o__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

3b8o__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	43.20 Å
Rg (electron density)	42.43 Å
Total Rg	42.78 Å
Atom count	14376
Residues	1704
Excluded volume	253860 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`3b8o__assembly_1__model_1`	octameric （8）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

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6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

Domain ID domain_id	d3b8oa1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d3b8ob_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d3b8oc_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d3b8od_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d3b8oe_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d3b8of_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d3b8og_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d3b8oh_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

CATH v4.4 (8 domains)

Domain ID domain_id	3b8oA01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	3b8oB01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	3b8oC01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	3b8oD01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	3b8oE01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	3b8oF01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	3b8oG01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	3b8oH01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

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Unit-cell length a length_a	107.438 Å
Unit-cell length b length_b	108.299 Å
Unit-cell length c length_c	122.569 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	102.15 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	2.400 Å
Low-resolution limit resolution_low	47.890 Å
R factor r_factor	0.2255
R_work r_work	0.2233
R_free r_free	0.2656

Description description	Lipopolysaccharide biosynthesis protein wzzE
Molecular weight formula_weight	30914.0 kDa
Source method src_method	man
Copy count entity_count	8

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H
Atom count atom_count	14771

Structure of WzzE- Bacterial Polysaccharide Co-polymerase

1. Protein Identity and Related Structures Protein Identity & Related Structures

Lipopolysaccharide biosynthesis protein wzzE

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

3b8o__assembly_1__model_1

3b8o__assembly_1__model_1 | I(q)

3b8o__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. Citations (1)

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	83334
Host organism host_organism	Escherichia coli BL21(DE3)

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	395

Structure of WzzE- Bacterial Polysaccharide Co-polymerase

1. Protein Identity and Related Structures Protein Identity & Related Structures

Lipopolysaccharide biosynthesis protein wzzE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

3b8o__assembly_1__model_1

3b8o__assembly_1__model_1 | I(q)

3b8o__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (8 domains)

CATH v4.4 (8 domains)

7. Citations (1)