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3dhg

Crystal Structure of Toluene 4-Monoxygenase Hydroxylase

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

toluene 4-monooxygenase hydroxylase alpha subunit

Pseudomonas mendocina

UniProt Q6Q8Q7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 toluene 4-monooxygenase hydroxylase beta subunit × 1 （Q6Q8Q3） toluene 4-monooxygenase hydroxylase gamma subunit × 1 （Q00457） FE (III) ION × 2 CALCIUM ION × 6 water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 3 toluene 4-monooxygenase hydroxylase beta subunit × 1 （Q6Q8Q3） toluene 4-monooxygenase hydroxylase gamma subunit × 1 （Q00457） FE (III) ION × 2 CALCIUM ION × 3 AZIDE ION × 1 water × 3 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 6 toluene 4-monooxygenase hydroxylase beta subunit × 2 （Q6Q8Q3） toluene 4-monooxygenase hydroxylase gamma subunit × 2 （Q00457） FE (III) ION × 4 CALCIUM ION × 9 AZIDE ION × 1 water × 6 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
3dhg 3dhg 3dhg 3dhh 3dhi 3dhi 3dhi 3ge3 3ge3 3ge8 3ge8 3ge8

19 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name Q6Q8Q7_PSEME
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–500； UniProt 1–500 Author chain D； PDBConstruct 1–500； UniProt 1–500

toluene 4-monooxygenase hydroxylase beta subunit

Pseudomonas mendocina

UniProt Q6Q8Q3

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 toluene 4-monooxygenase hydroxylase alpha subunit × 1 （Q6Q8Q7） toluene 4-monooxygenase hydroxylase gamma subunit × 1 （Q00457） FE (III) ION × 2 CALCIUM ION × 6 water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 3 toluene 4-monooxygenase hydroxylase alpha subunit × 1 （Q6Q8Q7） toluene 4-monooxygenase hydroxylase gamma subunit × 1 （Q00457） FE (III) ION × 2 CALCIUM ION × 3 AZIDE ION × 1 water × 3 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 6 toluene 4-monooxygenase hydroxylase alpha subunit × 2 （Q6Q8Q7） toluene 4-monooxygenase hydroxylase gamma subunit × 2 （Q00457） FE (III) ION × 4 CALCIUM ION × 9 AZIDE ION × 1 water × 6 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
3dhg 3dhg 3dhg 3dhh 3dhi 3dhi 3dhi

7 assemblies

View Construct and Data Evidence
UniProt name Q6Q8Q3_PSEME
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–327； UniProt 1–327 Author chain E； PDBConstruct 1–327； UniProt 1–327

toluene 4-monooxygenase hydroxylase gamma subunit

Pseudomonas mendocina

UniProt Q00457

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 toluene 4-monooxygenase hydroxylase alpha subunit × 1 （Q6Q8Q7） toluene 4-monooxygenase hydroxylase beta subunit × 1 （Q6Q8Q3） FE (III) ION × 2 CALCIUM ION × 6 water × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 3 toluene 4-monooxygenase hydroxylase alpha subunit × 1 （Q6Q8Q7） toluene 4-monooxygenase hydroxylase beta subunit × 1 （Q6Q8Q3） FE (III) ION × 2 CALCIUM ION × 3 AZIDE ION × 1 water × 3 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 6 toluene 4-monooxygenase hydroxylase alpha subunit × 2 （Q6Q8Q7） toluene 4-monooxygenase hydroxylase beta subunit × 2 （Q6Q8Q3） FE (III) ION × 4 CALCIUM ION × 9 AZIDE ION × 1 water × 6 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
3dhg 3dhg 3dhg 3dhh 3dhi 3dhi 3dhi 3ge3 3ge3 3ge8 3ge8 3ge8

28 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name TMOB_PSEME
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–84； UniProt 1–84 Author chain F； PDBConstruct 1–84； UniProt 1–84

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id3dhg
Deposition date deposition_date2008-06-17
Structure title titleCrystal Structure of Toluene 4-Monoxygenase Hydroxylase
Keywords keywordsmulticomponent monooxygenase, Aromatic hydrocarbons catabolism, FAD, Flavoprotein, Iron, Monooxygenase, Oxidoreductase; OXIDOREDUCTASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

3dhg__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

3dhg__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

3dhg__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）30.26 Å
Rg (electron density)29.57 Å
Total Rg30.29 Å
Atom count7220
Residues877
Excluded volume127450 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 3dhg__assembly_1__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 3dhg__assembly_2__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 3dhg__assembly_3__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

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6. Fold Classification (SCOP + CATH) 10 domains

SCOP 2.08 (4 domains)

Domain ID domain_idd3dhga_
Class classa — All alpha proteins
Fold Fold folda.25 — Ferritin-like
Superfamily Superfamily superfamilya.25.1 — Ferritin-like
Family Family familya.25.1.2 — Ribonucleotide reductase-like
Domain ID domain_idd3dhgc_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.12 — TmoB-like
Family Family familyd.15.12.0 — automated matches
Domain ID domain_idd3dhgd_
Class classa — All alpha proteins
Fold Fold folda.25 — Ferritin-like
Superfamily Superfamily superfamilya.25.1 — Ferritin-like
Family Family familya.25.1.2 — Ribonucleotide reductase-like
Domain ID domain_idd3dhgf_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.12 — TmoB-like
Family Family familyd.15.12.0 — automated matches

CATH v4.4 (6 domains)

Domain ID domain_id3dhgA00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology620 — Ribonucleotide Reductase, subunit A
Homologous superfamily homologous superfamily20 — Ribonucleotide Reductase, subunit A
Domain ID domain_id3dhgB00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology620 — Ribonucleotide Reductase, subunit A
Homologous superfamily homologous superfamily20 — Ribonucleotide Reductase, subunit A
Domain ID domain_id3dhgC00
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily270 — TmoB-like
Domain ID domain_id3dhgD00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology620 — Ribonucleotide Reductase, subunit A
Homologous superfamily homologous superfamily20 — Ribonucleotide Reductase, subunit A
Domain ID domain_id3dhgE00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology620 — Ribonucleotide Reductase, subunit A
Homologous superfamily homologous superfamily20 — Ribonucleotide Reductase, subunit A
Domain ID domain_id3dhgF00
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily270 — TmoB-like
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7. Citations (1)