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3g67

Crystal Structure of a Soluble Chemoreceptor from Thermotoga maritima

Method: X-RAY DIFFRACTION Dmax: 172.7 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Methyl-accepting chemotaxis protein

Thermotoga maritima

UniProt Q7DFA3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 41–253 Chain B； UniProt 41–253 Not recorded No other associated polymer X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.17 Å R-free 0.278

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 3G67 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3G6B Crystal structure of a Soluble Chemoreceptor from Thermotoga maritima Asn217Ile mutant Deposited 2009-02-06 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 41–253（213 aa）
Chain B 41–253（213 aa）
Mutation：N217I Mutation：N217I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.00 Å R-free 0.305
3UR1 The structure of a ternary complex between CheA domains P4 and P5 with CheW and with a truncated fragment of TM14, a chemoreceptor analog from Thermotoga maritima. Deposited 2011-11-21 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 107–191（85 aa） Fragment：unp residues 107-191
Chain D 107–191（85 aa） Fragment：unp residues 107-191
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 8.5；293 K；0.2 M sodium acetate trihydrate, 0.1 M Tris, 15% w/v Polyethylene glycol 4,000, pH 8.5, EVAPORATION, temperature 293K
Resolution 4.50 Å R-free 0.291
4JPB The structure of a ternary complex between CheA domains P4 and P5 with CheW and with an unzipped fragment of TM14, a chemoreceptor analog from Thermotoga maritima. Deposited 2013-03-19 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain B 107–192（86 aa）
Chain C 107–192（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Cubic shaped crystals (50x50x50 um3) were grown from a mixture of 520 uM Tm14s, 457 uM CheA 354 and 121 uM CheW after 1 month by vapor diffusion from a 2 ul drop (1:1 mixture of protein and reservoir: 500 ul reservoir of 0.2 M sodium acetate trihydrate, 0.1 M Tris (pH 8.5), 15% w/v polyethylene glycol 4,000), VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.19 Å R-free 0.220

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q7DFA3_THEMA
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–213； UniProt 41–253 Author chain B； PDBConstruct 1–213； UniProt 41–253

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 3g67

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 3g67
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2. Structure Basics 2. Structure Basics

Entry ID entry_id3g67
Deposition date deposition_date2009-02-06
Structure title titleCrystal Structure of a Soluble Chemoreceptor from Thermotoga maritima
Keywords keywordsFour-helix bundle, methyl-accepting chemotaxis protein, SIGNALING PROTEIN; SIGNALING PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier43.28 Å
Radius of gyration Rg (electron density) rg_electron45.23 Å
Forward intensity I(0) i038456400.00
Molecular weight molecular_weight48104.0 kDa
Excluded volume excluded_volume59815 ų
Envelope volume envelope_volume80161 ų
Hydration-shell volume shell_volume19909 ų
Envelope diameter envelope_diameter170.2 Å
Shell Rg shell_rg34.62 Å
Envelope Rg envelope_rg46.56 Å
Shape Rg shape_rg45.27 Å
Total Rg total_rg44.40 Å
Total atoms total_atoms3366
Residues n_residues425
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax172.7 Å
Rg (real space) rg_real44.53 Å
Rg uncertainty (real space) rg_real_error3.18 Å
I(0) (real space) i0_real3.8460e+07
I(0) uncertainty (real space) i0_real_error8.5290e+05
Rg (reciprocal space) rg_reciprocal43.29 Å
I(0) (reciprocal space) i0_reciprocal38400000.0000
Solution quality estimate total_estimate0.5430
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks3
Primary peak position r_peak_primary24.0 Å
Skewness Skewness skewness0.698
Kurtosis Kurtosis kurtosis-0.342
Angular range angular_range— – 0.1800 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha1037000.0000
Real-space data points n_real_points37
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.018; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.004; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

Domain ID domain_id3g67A00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily950 — Methyl-accepting chemotaxis protein
Domain ID domain_id3g67B00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily950 — Methyl-accepting chemotaxis protein
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8. Citations (1)

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9. Files and Curves (10)