3uq5

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Gamma-aminobutyric-acid receptor subunit beta-1

Erwinia chrysanthemi

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 5	No other associated polymer	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 5	SODIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	E0SJQ4_DICD3
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 3–324； UniProt 22–343 Author chain B； PDBConstruct 3–324； UniProt 22–343 Author chain C； PDBConstruct 3–324； UniProt 22–343 Author chain D； PDBConstruct 3–324； UniProt 22–343 Author chain E； PDBConstruct 3–324； UniProt 22–343 Author chain F； PDBConstruct 3–324； UniProt 22–343 Author chain G； PDBConstruct 3–324； UniProt 22–343 Author chain H； PDBConstruct 3–324； UniProt 22–343 Author chain I； PDBConstruct 3–324； UniProt 22–343 Author chain J； PDBConstruct 3–324； UniProt 22–343

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	3uq5
Deposition date deposition_date	2011-11-19
Structure title title	X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine
Keywords keywords	membrane protein, liganded-gated ion channel, TRANSPORT PROTEIN; TRANSPORT PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

3uq5__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

3uq5__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

3uq5__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	37.74 Å
Rg (electron density)	36.84 Å
Total Rg	37.42 Å
Atom count	12495
Residues	1535
Excluded volume	224040 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`3uq5__assembly_1__model_1`	pentameric （5）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`3uq5__assembly_2__model_1`	pentameric （5）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

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Unit-cell length a length_a	105.570 Å
Unit-cell length b length_b	267.430 Å
Unit-cell length c length_c	111.160 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	108.64 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	20

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	4.200 Å
Low-resolution limit resolution_low	19.988 Å
R factor r_factor	0.2213
R_work r_work	0.2198
R_free r_free	0.2494

Description description	Gamma-aminobutyric-acid receptor subunit beta-1
Molecular weight formula_weight	36947.0 kDa
Mutation mutation	L239A,F246L
Source method src_method	man
Copy count entity_count	10

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H,I,J
Atom count atom_count	24991

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine

1. Protein Identity and Related Structures Protein Identity & Related Structures

Gamma-aminobutyric-acid receptor subunit beta-1

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

3uq5__assembly_1__model_1

3uq5__assembly_1__model_1 | I(q)

3uq5__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

7. Citations (1)

Source organism organism	Erwinia chrysanthemi
NCBI taxonomy ID ncbi_taxonomy_id	198628
Host organism host_organism	Escherichia coli

Description description	SODIUM ION
Molecular weight formula_weight	23.0 kDa
Source method src_method	syn
Copy count entity_count	1

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine

1. Protein Identity and Related Structures Protein Identity & Related Structures

Gamma-aminobutyric-acid receptor subunit beta-1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

3uq5__assembly_1__model_1

3uq5__assembly_1__model_1 | I(q)

3uq5__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

7. Citations (1)