4e2l

Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ferric enterobactin (Enterochelin) transport

Escherichia coli

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 9	No other associated polymer	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q8XBV8_ECO57
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 9–201； UniProt 65–257 Author chain A； PDBConstruct 205–271； UniProt 265–331 Author chain B； PDBConstruct 9–201； UniProt 65–257 Author chain B； PDBConstruct 205–271； UniProt 265–331 Author chain C； PDBConstruct 9–201； UniProt 65–257 Author chain C； PDBConstruct 205–271； UniProt 265–331 Author chain D； PDBConstruct 9–201； UniProt 65–257 Author chain D； PDBConstruct 205–271； UniProt 265–331 Author chain E； PDBConstruct 9–201； UniProt 65–257 Author chain E； PDBConstruct 205–271； UniProt 265–331 Author chain F； PDBConstruct 9–201； UniProt 65–257 Author chain F； PDBConstruct 205–271； UniProt 265–331 Author chain G； PDBConstruct 9–201； UniProt 65–257 Author chain G； PDBConstruct 205–271； UniProt 265–331 Author chain H； PDBConstruct 9–201； UniProt 65–257 Author chain H； PDBConstruct 205–271； UniProt 265–331 Author chain I； PDBConstruct 9–201； UniProt 65–257 Author chain I； PDBConstruct 205–271； UniProt 265–331

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	4e2l
Deposition date deposition_date	2012-03-08
Structure title title	Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein
Keywords keywords	FepE polysaccharride co-polymerase, wzz, inner membrane, periplasmic space, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

4e2l__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

4e2l__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

4e2l__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	48.57 Å
Rg (electron density)	47.70 Å
Total Rg	48.01 Å
Atom count	16883
Residues	2175
Excluded volume	302340 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`4e2l__assembly_1__model_1`	nonameric （9）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (1)

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6. Fold Classification (SCOP + CATH) 27 domains

SCOP 2.08 (9 domains)

Domain ID domain_id	d4e2la_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d4e2lb_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d4e2lc_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d4e2ld_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d4e2le_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d4e2lf_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d4e2lg_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d4e2lh_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

Domain ID domain_id	d4e2li_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.60 — Bacterial polysaccharide co-polymerase-like
Family Family family	d.58.60.1 — FepE-like

CATH v4.4 (18 domains)

Domain ID domain_id	4e2lA01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	4e2lA02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	210 —

Domain ID domain_id	4e2lB01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	4e2lB02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	210 —

Domain ID domain_id	4e2lC01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	4e2lC02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	210 —

Domain ID domain_id	4e2lD01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	4e2lD02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	210 —

Domain ID domain_id	4e2lE01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	4e2lE02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	210 —

Domain ID domain_id	4e2lF01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	4e2lF02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	210 —

Domain ID domain_id	4e2lG01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	4e2lG02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	210 —

Domain ID domain_id	4e2lH01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	4e2lH02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	210 —

Domain ID domain_id	4e2lI01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1890 — Bacterial polysaccharide co-polymerase-like
Homologous superfamily homologous superfamily	10 — FepE-like

Domain ID domain_id	4e2lI02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	210 —

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Unit-cell length a length_a	208.307 Å
Unit-cell length b length_b	141.257 Å
Unit-cell length c length_c	136.888 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	107.03 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	36

Space group space_group	C 1 2 1
Space-group number int_tables_number	5
Crystal system crystal_system	—

Resolution resolution	2.800 Å
Low-resolution limit resolution_low	49.716 Å
R factor r_factor	0.2440
R_work r_work	0.2417
R_free r_free	0.2891

Description description	Ferric enterobactin (Enterochelin) transport
Molecular weight formula_weight	30888.9 kDa
Mutation mutation	delta(258-264)
Source method src_method	man
Copy count entity_count	9

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H,I
Atom count atom_count	16883

Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein

1. Protein Identity and Related Structures Protein Identity & Related Structures

Ferric enterobactin (Enterochelin) transport

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4e2l__assembly_1__model_1

4e2l__assembly_1__model_1 | I(q)

4e2l__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

6. Fold Classification (SCOP + CATH) 27 domains

SCOP 2.08 (9 domains)

CATH v4.4 (18 domains)

7. Citations (1)

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	83334
Host organism host_organism	Escherichia coli

Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein

1. Protein Identity and Related Structures Protein Identity & Related Structures

Ferric enterobactin (Enterochelin) transport

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4e2l__assembly_1__model_1

4e2l__assembly_1__model_1 | I(q)

4e2l__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

6. Fold Classification (SCOP + CATH) 27 domains

SCOP 2.08 (9 domains)

CATH v4.4 (18 domains)

7. Citations (1)