4fa4

Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 10 Days

Method: X-RAY DIFFRACTION D_max: 162.5 Å Quality: GOOD

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Methylamine utilization protein MauG

Paracoccus denitrificans

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 6 PDB 声明：hexameric（6）与蛋白拷贝数一致	链 A； UniProt 21–387 链 B； UniProt 21–387	片段：unp residues 21-387	Methylamine dehydrogenase light chain × 2 （P22619） Methylamine dehydrogenase heavy chain × 2 （A1BB97） CA CALCIUM ION × 2 HEC HEME C × 4 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 2 NA SODIUM ION × 3 PO4 PHOSPHATE ION × 1 ACT ACETATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K , VAPOR DIFFUSION, HANGING DROP	分辨率 2.14 Å R-free 0.227

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 4FA4	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
3L4M Crystal Structure of the MauG/pre-Methylamine Dehydrogenase Complex. 提交 2009-12-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K	分辨率 2.02 Å R-free 0.189
3L4O Crystal Structure of the MauG/pre-Methylamine Dehydrogenase Complex After Treatment with Hydrogen Peroxide 提交 2009-12-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000; followed by reaction of the crystal with hydrogen peroxide immediately prior to flash cooling., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.05 Å R-free 0.194
3ORV Crystal Structure of the Y294H-MauG/pre-Methylamine Dehydrogenase Complex 提交 2010-09-07	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	突变：Y294H 突变：Y294H	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 PG4 TETRAETHYLENE GLYCOL × 2 P6G HEXAETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；Drops contained 1uL protein with 2uL reservoir solution. Protein solution: 100uM Y294H-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 24-26 % w/v PEG 8000, 0.1 M sodium acetate, 0.1 M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.91 Å R-free 0.187
3PXS Crystal Structure of Diferrous MauG in Complex with Pre-Methylamine Dehydrogenase: 提交 2010-12-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	未记录	NA SODIUM ION × 4 HEC HEME C × 4 CA CALCIUM ION × 2 ACT ACETATE ION × 3 PG4 TETRAETHYLENE GLYCOL × 1 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 23-25 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K , VAPOR DIFFUSION, HANGING DROP	分辨率 2.22 Å R-free 0.229
3PXT Crystal Structure of Ferrous CO Adduct of MauG in Complex with Pre-Methylamine Dehydrogenase 提交 2010-12-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	未记录	NA SODIUM ION × 3 CMO CARBON MONOXIDE × 2 HEC HEME C × 4 CA CALCIUM ION × 2 ACT ACETATE ION × 3 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 23-25 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP	分辨率 2.16 Å R-free 0.226
3PXW Crystal Structure of Ferrous NO Adduct of MauG in Complex with Pre-Methylamine Dehydrogenase 提交 2010-12-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	未记录	NA SODIUM ION × 2 NO NITRIC OXIDE × 2 HEC HEME C × 4 CA CALCIUM ION × 2 ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 1 PGE TRIETHYLENE GLYCOL × 1 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 23-35 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP	分辨率 2.11 Å R-free 0.242
3RLM Structure of the W199F MauG/pre-Methylamine Dehydrogenase complex after treatment with hydrogen peroxide 提交 2011-04-19	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	突变：W199F 突变：W199F	CA CALCIUM ION × 2 HEC HEME C × 4 PGE TRIETHYLENE GLYCOL × 1 ACT ACETATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES, 0.1M sodium acetate, 23-25% w/v PEG 8000, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.13 Å R-free 0.237
3RMZ Crystal Structure of the W199F-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-04-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	突变：W199F 突变：W199F	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 2 PEG DI(HYDROXYETHYL)ETHER × 1 ACT ACETATE ION × 1 PG4 TETRAETHYLENE GLYCOL × 1 P6G HEXAETHYLENE GLYCOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM W199F-MauG and 50uM preMADH in 10mM potassium phosphate, pH 7.5. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.72 Å R-free 0.181
3RN0 Crystal Structure of the W199K-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-04-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	突变：W199K 突变：W199K	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 P6G HEXAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；Drops contained 1uL protein with 2.5uL reservoir solution. Protein solution: 100uM W199K-MauG and 50uM preMADH in 10mM potassium phosphate, pH 7.5. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.91 Å R-free 0.230
3RN1 Crystal Structure of the W199E-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-04-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	突变：W199E 突变：W199E	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 1 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM W199E-MauG and 50uM preMADH in 10mM potassium phosphate pH, 7.5. Reservoir solution contained: 23% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.93 Å R-free 0.192
3SJL Crystal Structure of the P107S-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-06-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	突变：P107S 突变：P107S	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 PEG DI(HYDROXYETHYL)ETHER × 3 EDO 1,2-ETHANEDIOL × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM P107S-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.63 Å R-free 0.180
3SLE Crystal Structure of the P107C-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-06-24	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	突变：P107C 非标准单体：是（mmCIF未提供具体位点）突变：P107C 非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 ACT ACETATE ION × 4 EDO 1,2-ETHANEDIOL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP	分辨率 2.52 Å R-free 0.243
3SVW Crystal Structure of the P107V-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-07-12	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	突变：P107V 突变：P107V	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 EDO 1,2-ETHANEDIOL × 3 ACT ACETATE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM P107V-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 23% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.86 Å R-free 0.174
3SWS Crystal Structure of the Quinone Form of Methylamine Dehydrogenase in Complex with the Diferric Form of MauG 提交 2011-07-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	未记录	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 ACT ACETATE ION × 1 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM WT-MauG and 50uM MADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 25% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.2., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.86 Å R-free 0.178
3SXT Crystal Structure of the Quinol Form of Methylamine Dehydrogenase in Complex with the Diferrous Form of MauG 提交 2011-07-15	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	未记录	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 5	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. WT-MauG and MADH were each reduced in an anaerobic glove box prior to preparing the protein mixture for crystallization. Protein mixture: 100uM reduced WT-MauG and 50uM reduced MADH in 10mM potassium phosphate pH7.5 with 2mM sodium dithionite. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4 and 2mM sodium dithionite. Crystallization was carried out in an anaerobic glove box at ambient temperature., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.81 Å R-free 0.187
4FA1 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 130 Days. 提交 2012-05-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 2 EDO 1,2-ETHANEDIOL × 2 ACT ACETATE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.18 Å R-free 0.227
4FA5 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 20 Days 提交 2012-05-21	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：unp residues 21-387 链 B 21–387（367 aa）片段：unp residues 21-387	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 NA SODIUM ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.94 Å R-free 0.204
4FA9 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 30 Days 提交 2012-05-21	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：unp residues 21-387 链 B 21–387（367 aa）片段：unp residues 21-387	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 ACT ACETATE ION × 5 NA SODIUM ION × 4 EDO 1,2-ETHANEDIOL × 1 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.09 Å R-free 0.214
4FAN Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 40 Days 提交 2012-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：unp residues 21-387 链 B 21–387（367 aa）片段：unp residues 21-387	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 ACT ACETATE ION × 1 NA SODIUM ION × 2 EDO 1,2-ETHANEDIOL × 1 PGE TRIETHYLENE GLYCOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.08 Å R-free 0.234
4FAV Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 50 Days 提交 2012-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：unp residues 21-387 链 B 21–387（367 aa）片段：unp residues 21-387	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 2 EDO 1,2-ETHANEDIOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.08 Å R-free 0.224
4FB1 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 60 Days 提交 2012-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：unp residues 21-387 链 B 21–387（367 aa）片段：unp residues 21-387	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.15 Å R-free 0.222
4K3I Crystal Structure of the Quinol Form of Methylamine Dehydrogenase in Complex with the Diferrous Form of MauG, C2 Space Group 提交 2013-04-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 4 EDO 1,2-ETHANEDIOL × 4 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. WT-MauG and MADH were each reduced in an anaerobic glove box prior to preparing the protein mixture for crystallization. Protein mixture: 100uM reduced WT-MauG and 50uM reduced MADH in 10mM potassium phosphate pH7.5 with 2mM sodium dithionite. Reservoir solution contained: 24% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4 and 2mM sodium dithionite. Crystallization was carried out in an anaerobic glove box at ambient temperature., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.00 Å R-free 0.190
4L1Q Crystal Structure of the E113Q-MauG/pre-Methylamine Dehydrogenase Complex 提交 2013-06-03	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	突变：E113Q 突变：E113Q	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 NA SODIUM ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30% w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.92 Å R-free 0.207
4L3G Crystal Structure of the E113Q-MauG/pre-Methylamine Dehydrogenase Complex Aged 120 Days 提交 2013-06-05	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	突变：E113Q 非标准单体：是（mmCIF未提供具体位点）突变：E113Q 非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 4 ACT ACETATE ION × 2 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30% w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.05 Å R-free 0.210
4L3H Crystal Structure of the E113Q-MauG/pre-Methylamine Dehydrogenase Complex After Treatment with Hydrogen Peroxide 提交 2013-06-05	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）链 B 21–387（367 aa）	突变：E113Q 突变：E113Q	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 4 EDO 1,2-ETHANEDIOL × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.79 Å R-free 0.198
4O1Q Crystal Structure of the Q103N-MauG/pre-Methylamine Dehydrogenase Complex 提交 2013-12-16	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 21–387（367 aa）片段：UNP residues 21-387 链 B 21–387（367 aa）片段：UNP residues 21-387	突变：Q103N 突变：Q103N	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 PO4 PHOSPHATE ION × 1 PGE TRIETHYLENE GLYCOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 NA SODIUM ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1 M MES, pH 6.4, 0.1 M sodium acetate, 24-30% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.59 Å R-free 0.254
4Y5R Crystal Structure of a T67A MauG/pre-Methylamine Dehydrogenase Complex 提交 2015-02-11	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 A 26–380（355 aa）片段：UNP RESIDUES 26-380 链 B 26–380（355 aa）片段：UNP RESIDUES 26-380	突变：T67A 突变：T67A	CA CALCIUM ION × 2 HEC HEME C × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；292 K；25-30% PEG 8000, 0,1 M sodium Acetate, 0.1 M MES pH 6.4	分辨率 2.80 Å R-free 0.256

共 27 个其他 PDB 条目、27 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	MAUG_PARDP
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–367； UniProt 21–387 作者链 B； PDB构建体 1–367； UniProt 21–387

Methylamine dehydrogenase light chain

Paracoccus denitrificans

UniProt P22619

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 6 PDB 声明：hexameric（6）与蛋白拷贝数一致	链 C； UniProt 58–188 链 E； UniProt 58–188	片段：unp residues 58-188 非标准单体：是（mmCIF未提供具体位点）	Methylamine utilization protein MauG × 2 （Q51658） Methylamine dehydrogenase heavy chain × 2 （A1BB97） CA CALCIUM ION × 2 HEC HEME C × 4 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 2 NA SODIUM ION × 3 PO4 PHOSPHATE ION × 1 ACT ACETATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K , VAPOR DIFFUSION, HANGING DROP	分辨率 2.14 Å R-free 0.227

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 4FA4	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1MG2 MUTATION OF ALPHA PHE55 OF METHYLAMINE DEHYDROGENASE ALTERS THE REORGANIZATION ENERGY AND ELECTRONIC COUPLING FOR ITS ELECTRON TRANSFER REACTION WITH AMICYANIN 提交 2002-08-14	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 B 58–188（131 aa）链 F 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	PO4 PHOSPHATE ION × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEC HEME C × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5；295 K；phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 2.25 Å R-free 0.210
1MG2 MUTATION OF ALPHA PHE55 OF METHYLAMINE DEHYDROGENASE ALTERS THE REORGANIZATION ENERGY AND ELECTRONIC COUPLING FOR ITS ELECTRON TRANSFER REACTION WITH AMICYANIN 提交 2002-08-14	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 J 58–188（131 aa）链 N 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	PO4 PHOSPHATE ION × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEC HEME C × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5；295 K；phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 2.25 Å R-free 0.210
1MG3 MUTATION OF ALPHA PHE55 OF METHYLAMINE DEHYDROGENASE ALTERS THE REORGANIZATION ENERGY AND ELECTRONIC COUPLING FOR ITS ELECTRON TRANSFER REACTION WITH AMICYANIN 提交 2002-08-14	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 B 58–188（131 aa）链 F 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	PO4 PHOSPHATE ION × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEC HEME C × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5；295 K；phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 2.40 Å R-free 0.246
1MG3 MUTATION OF ALPHA PHE55 OF METHYLAMINE DEHYDROGENASE ALTERS THE REORGANIZATION ENERGY AND ELECTRONIC COUPLING FOR ITS ELECTRON TRANSFER REACTION WITH AMICYANIN 提交 2002-08-14	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 J 58–188（131 aa）链 N 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	PO4 PHOSPHATE ION × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEC HEME C × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5；295 K；phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 2.40 Å R-free 0.246
2BBK CRYSTAL STRUCTURE OF THE QUINOPROTEIN METHYLAMINE DEHYDROGENASE FROM PARACOCCUS DENITRIFICANS AT 1.75 ANGSTROMS 提交 1993-12-17	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 L 64–188（125 aa）链 M 64–188（125 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 1.75 Å
2GC4 Structural comparison of the oxidized ternary electron transfer complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans with the substrate-reduced, copper free complex at 1.9 A resolution. 提交 2006-03-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 B 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）	CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEC HEME C × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；2.3-2.6M Sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 1.90 Å R-free 0.197
2GC4 Structural comparison of the oxidized ternary electron transfer complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans with the substrate-reduced, copper free complex at 1.9 A resolution. 提交 2006-03-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 F 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）	CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEC HEME C × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；2.3-2.6M Sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 1.90 Å R-free 0.197
2GC4 Structural comparison of the oxidized ternary electron transfer complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans with the substrate-reduced, copper free complex at 1.9 A resolution. 提交 2006-03-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 J 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）	CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEC HEME C × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；2.3-2.6M Sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 1.90 Å R-free 0.197
2GC4 Structural comparison of the oxidized ternary electron transfer complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans with the substrate-reduced, copper free complex at 1.9 A resolution. 提交 2006-03-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 N 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）	CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEC HEME C × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；2.3-2.6M Sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 1.90 Å R-free 0.197
2GC7 Substrate reduced, copper free complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans. 提交 2006-03-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 B 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）	NA SODIUM ION × 1 HEC HEME C × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；2.3-2.6M sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 1.90 Å R-free 0.198
2GC7 Substrate reduced, copper free complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans. 提交 2006-03-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 F 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）	NA SODIUM ION × 1 HEC HEME C × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；2.3-2.6M sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 1.90 Å R-free 0.198
2GC7 Substrate reduced, copper free complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans. 提交 2006-03-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 J 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）	NA SODIUM ION × 1 HEC HEME C × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；2.3-2.6M sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 1.90 Å R-free 0.198
2GC7 Substrate reduced, copper free complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans. 提交 2006-03-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 N 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）	NA SODIUM ION × 1 HEC HEME C × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；2.3-2.6M sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K	分辨率 1.90 Å R-free 0.198
2J55 X-ray reduced Paraccocus denitrificans methylamine dehydrogenase O- quinone in complex with amicyanin. 提交 2006-09-12	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 L 58–188（131 aa）链 M 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CU COPPER (II) ION × 2 GOL GLYCEROL × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 5.6；pH 5.60	分辨率 2.15 Å R-free 0.245
2J56 X-ray reduced Paraccocus denitrificans methylamine dehydrogenase N- semiquinone in complex with amicyanin. 提交 2006-09-12	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 L 58–188（131 aa）链 M 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CU COPPER (II) ION × 2 GOL GLYCEROL × 1 NA SODIUM ION × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 9；pH 9.00	分辨率 2.10 Å R-free 0.207
2J57 X-ray reduced Paraccocus denitrificans methylamine dehydrogenase N- quinol in complex with amicyanin. 提交 2006-09-12	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 L 58–188（131 aa）链 M 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CU COPPER (II) ION × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 5.6；pH 5.60	分辨率 2.25 Å R-free 0.241
2J57 X-ray reduced Paraccocus denitrificans methylamine dehydrogenase N- quinol in complex with amicyanin. 提交 2006-09-12	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 K 58–188（131 aa）链 N 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CU COPPER (II) ION × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 5.6；pH 5.60	分辨率 2.25 Å R-free 0.241
2MTA CRYSTAL STRUCTURE OF A TERNARY ELECTRON TRANSFER COMPLEX BETWEEN METHYLAMINE DEHYDROGENASE, AMICYANIN AND A C-TYPE CYTOCHROME 提交 1993-10-26	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 L 64–188（125 aa）	非标准单体：是（mmCIF未提供具体位点）	PO4 PHOSPHATE ION × 2 CU COPPER (II) ION × 2 HEC HEME C × 2	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.40 Å
3L4M Crystal Structure of the MauG/pre-Methylamine Dehydrogenase Complex. 提交 2009-12-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：Beta chain of immature methylamine dehydrogenase (preMADH) 链 E 58–188（131 aa）片段：Beta chain of immature methylamine dehydrogenase (preMADH)	突变：Trp57 is hydroxylated at C7 非标准单体：是（mmCIF未提供具体位点）突变：Trp57 is hydroxylated at C7 非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K	分辨率 2.02 Å R-free 0.189
3L4O Crystal Structure of the MauG/pre-Methylamine Dehydrogenase Complex After Treatment with Hydrogen Peroxide 提交 2009-12-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：Beta chain of immature methylamine dehydrogenase (preMADH) 链 E 58–188（131 aa）片段：Beta chain of immature methylamine dehydrogenase (preMADH)	突变：Hydroxylated Trp57 has been converted to the full-quinone form due to hydrogen peroxide-assisted catalysis by MauG in the crystal. 非标准单体：是（mmCIF未提供具体位点）突变：Hydroxylated Trp57 has been converted to the full-quinone form due to hydrogen peroxide-assisted catalysis by MauG in the crystal. 非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000; followed by reaction of the crystal with hydrogen peroxide immediately prior to flash cooling., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.05 Å R-free 0.194
3ORV Crystal Structure of the Y294H-MauG/pre-Methylamine Dehydrogenase Complex 提交 2010-09-07	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	突变：Trp57 is hydroxylated at C7 非标准单体：是（mmCIF未提供具体位点）突变：Trp57 is hydroxylated at C7 非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 PG4 TETRAETHYLENE GLYCOL × 2 P6G HEXAETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；Drops contained 1uL protein with 2uL reservoir solution. Protein solution: 100uM Y294H-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 24-26 % w/v PEG 8000, 0.1 M sodium acetate, 0.1 M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.91 Å R-free 0.187
3PXS Crystal Structure of Diferrous MauG in Complex with Pre-Methylamine Dehydrogenase: 提交 2010-12-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	NA SODIUM ION × 4 HEC HEME C × 4 CA CALCIUM ION × 2 ACT ACETATE ION × 3 PG4 TETRAETHYLENE GLYCOL × 1 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 23-25 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K , VAPOR DIFFUSION, HANGING DROP	分辨率 2.22 Å R-free 0.229
3PXT Crystal Structure of Ferrous CO Adduct of MauG in Complex with Pre-Methylamine Dehydrogenase 提交 2010-12-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	NA SODIUM ION × 3 CMO CARBON MONOXIDE × 2 HEC HEME C × 4 CA CALCIUM ION × 2 ACT ACETATE ION × 3 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 23-25 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP	分辨率 2.16 Å R-free 0.226
3PXW Crystal Structure of Ferrous NO Adduct of MauG in Complex with Pre-Methylamine Dehydrogenase 提交 2010-12-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	NA SODIUM ION × 2 NO NITRIC OXIDE × 2 HEC HEME C × 4 CA CALCIUM ION × 2 ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 1 PGE TRIETHYLENE GLYCOL × 1 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 23-35 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP	分辨率 2.11 Å R-free 0.242
3SJL Crystal Structure of the P107S-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-06-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	突变：Trp57 is hydroxylated at C7 非标准单体：是（mmCIF未提供具体位点）突变：Trp57 is hydroxylated at C7 非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 PEG DI(HYDROXYETHYL)ETHER × 3 EDO 1,2-ETHANEDIOL × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM P107S-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.63 Å R-free 0.180
3SLE Crystal Structure of the P107C-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-06-24	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 ACT ACETATE ION × 4 EDO 1,2-ETHANEDIOL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP	分辨率 2.52 Å R-free 0.243
3SVW Crystal Structure of the P107V-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-07-12	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 EDO 1,2-ETHANEDIOL × 3 ACT ACETATE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM P107V-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 23% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.86 Å R-free 0.174
3SWS Crystal Structure of the Quinone Form of Methylamine Dehydrogenase in Complex with the Diferric Form of MauG 提交 2011-07-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 ACT ACETATE ION × 1 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM WT-MauG and 50uM MADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 25% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.2., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.86 Å R-free 0.178
3SXT Crystal Structure of the Quinol Form of Methylamine Dehydrogenase in Complex with the Diferrous Form of MauG 提交 2011-07-15	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 5	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. WT-MauG and MADH were each reduced in an anaerobic glove box prior to preparing the protein mixture for crystallization. Protein mixture: 100uM reduced WT-MauG and 50uM reduced MADH in 10mM potassium phosphate pH7.5 with 2mM sodium dithionite. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4 and 2mM sodium dithionite. Crystallization was carried out in an anaerobic glove box at ambient temperature., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.81 Å R-free 0.187
4FA1 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 130 Days. 提交 2012-05-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）链 E 58–188（131 aa）	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 2 EDO 1,2-ETHANEDIOL × 2 ACT ACETATE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.18 Å R-free 0.227
4FA5 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 20 Days 提交 2012-05-21	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：unp residues 58-188 链 E 58–188（131 aa）片段：unp residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 NA SODIUM ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.94 Å R-free 0.204
4FA9 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 30 Days 提交 2012-05-21	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：unp residues 58-188 链 E 58–188（131 aa）片段：unp residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 ACT ACETATE ION × 5 NA SODIUM ION × 4 EDO 1,2-ETHANEDIOL × 1 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.09 Å R-free 0.214
4FAN Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 40 Days 提交 2012-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：unp residues 58-188 链 E 58–188（131 aa）片段：unp residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 ACT ACETATE ION × 1 NA SODIUM ION × 2 EDO 1,2-ETHANEDIOL × 1 PGE TRIETHYLENE GLYCOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.08 Å R-free 0.234
4FAV Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 50 Days 提交 2012-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：unp residues 58-188 链 E 58–188（131 aa）片段：unp residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 2 EDO 1,2-ETHANEDIOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.08 Å R-free 0.224
4FB1 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 60 Days 提交 2012-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：unp residues 58-188 链 E 58–188（131 aa）片段：unp residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.15 Å R-free 0.222
4K3I Crystal Structure of the Quinol Form of Methylamine Dehydrogenase in Complex with the Diferrous Form of MauG, C2 Space Group 提交 2013-04-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 58–188（131 aa）片段：UNP residues 58-188 链 E 58–188（131 aa）片段：UNP residues 58-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 4 EDO 1,2-ETHANEDIOL × 4 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. WT-MauG and MADH were each reduced in an anaerobic glove box prior to preparing the protein mixture for crystallization. Protein mixture: 100uM reduced WT-MauG and 50uM reduced MADH in 10mM potassium phosphate pH7.5 with 2mM sodium dithionite. Reservoir solution contained: 24% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4 and 2mM sodium dithionite. Crystallization was carried out in an anaerobic glove box at ambient temperature., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.00 Å R-free 0.190
4Y5R Crystal Structure of a T67A MauG/pre-Methylamine Dehydrogenase Complex 提交 2015-02-11	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 C 64–188（125 aa）片段：UNP RESIDUES 64-188 链 E 64–188（125 aa）片段：UNP RESIDUES 64-188	非标准单体：是（mmCIF未提供具体位点）非标准单体：是（mmCIF未提供具体位点）	CA CALCIUM ION × 2 HEC HEME C × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；292 K；25-30% PEG 8000, 0,1 M sodium Acetate, 0.1 M MES pH 6.4	分辨率 2.80 Å R-free 0.256

共 28 个其他 PDB 条目、37 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	DHML_PARDE
Isoform	—
PDB实体	2
链与序列区间	作者链 C； PDB构建体 1–131； UniProt 58–188 作者链 E； PDB构建体 1–131； UniProt 58–188

Methylamine dehydrogenase heavy chain

Paracoccus denitrificans

UniProt A1BB97

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 6 PDB 声明：hexameric（6）与蛋白拷贝数一致	链 D； UniProt 33–417 链 F； UniProt 33–417	片段：unp residues 33-417	Methylamine utilization protein MauG × 2 （Q51658） Methylamine dehydrogenase light chain × 2 （P22619） CA CALCIUM ION × 2 HEC HEME C × 4 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 2 NA SODIUM ION × 3 PO4 PHOSPHATE ION × 1 ACT ACETATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K , VAPOR DIFFUSION, HANGING DROP	分辨率 2.14 Å R-free 0.227

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 4FA4	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
3L4M Crystal Structure of the MauG/pre-Methylamine Dehydrogenase Complex. 提交 2009-12-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）链 F 32–417（386 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K	分辨率 2.02 Å R-free 0.189
3L4O Crystal Structure of the MauG/pre-Methylamine Dehydrogenase Complex After Treatment with Hydrogen Peroxide 提交 2009-12-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）链 F 32–417（386 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000; followed by reaction of the crystal with hydrogen peroxide immediately prior to flash cooling., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.05 Å R-free 0.194
3ORV Crystal Structure of the Y294H-MauG/pre-Methylamine Dehydrogenase Complex 提交 2010-09-07	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）片段：UNP residues 32-417 链 F 32–417（386 aa）片段：UNP residues 32-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 PG4 TETRAETHYLENE GLYCOL × 2 P6G HEXAETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；Drops contained 1uL protein with 2uL reservoir solution. Protein solution: 100uM Y294H-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 24-26 % w/v PEG 8000, 0.1 M sodium acetate, 0.1 M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.91 Å R-free 0.187
3PXS Crystal Structure of Diferrous MauG in Complex with Pre-Methylamine Dehydrogenase: 提交 2010-12-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）片段：UNP residues 32-417 链 F 32–417（386 aa）片段：UNP residues 32-417	未记录	NA SODIUM ION × 4 HEC HEME C × 4 CA CALCIUM ION × 2 ACT ACETATE ION × 3 PG4 TETRAETHYLENE GLYCOL × 1 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 23-25 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K , VAPOR DIFFUSION, HANGING DROP	分辨率 2.22 Å R-free 0.229
3PXT Crystal Structure of Ferrous CO Adduct of MauG in Complex with Pre-Methylamine Dehydrogenase 提交 2010-12-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）片段：UNP residues 32-417 链 F 32–417（386 aa）片段：UNP residues 32-417	未记录	NA SODIUM ION × 3 CMO CARBON MONOXIDE × 2 HEC HEME C × 4 CA CALCIUM ION × 2 ACT ACETATE ION × 3 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 23-25 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP	分辨率 2.16 Å R-free 0.226
3PXW Crystal Structure of Ferrous NO Adduct of MauG in Complex with Pre-Methylamine Dehydrogenase 提交 2010-12-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）片段：UNP residues 32-417 链 F 33–417（385 aa）片段：UNP residues 32-417	未记录	NA SODIUM ION × 2 NO NITRIC OXIDE × 2 HEC HEME C × 4 CA CALCIUM ION × 2 ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 1 PGE TRIETHYLENE GLYCOL × 1 PG6 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 23-35 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP	分辨率 2.11 Å R-free 0.242
3RLM Structure of the W199F MauG/pre-Methylamine Dehydrogenase complex after treatment with hydrogen peroxide 提交 2011-04-19	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）链 F 32–417（386 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 PGE TRIETHYLENE GLYCOL × 1 ACT ACETATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES, 0.1M sodium acetate, 23-25% w/v PEG 8000, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.13 Å R-free 0.237
3RMZ Crystal Structure of the W199F-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-04-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）链 F 32–417（386 aa）	未记录	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 2 PEG DI(HYDROXYETHYL)ETHER × 1 ACT ACETATE ION × 1 PG4 TETRAETHYLENE GLYCOL × 1 P6G HEXAETHYLENE GLYCOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM W199F-MauG and 50uM preMADH in 10mM potassium phosphate, pH 7.5. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.72 Å R-free 0.181
3RN0 Crystal Structure of the W199K-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-04-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）链 F 32–417（386 aa）	未记录	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 P6G HEXAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；Drops contained 1uL protein with 2.5uL reservoir solution. Protein solution: 100uM W199K-MauG and 50uM preMADH in 10mM potassium phosphate, pH 7.5. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.91 Å R-free 0.230
3RN1 Crystal Structure of the W199E-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-04-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）链 F 32–417（386 aa）	未记录	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 1 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM W199E-MauG and 50uM preMADH in 10mM potassium phosphate pH, 7.5. Reservoir solution contained: 23% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.93 Å R-free 0.192
3SJL Crystal Structure of the P107S-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-06-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）片段：UNP residues 32-417 链 F 32–417（386 aa）片段：UNP residues 32-417	未记录	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 PEG DI(HYDROXYETHYL)ETHER × 3 EDO 1,2-ETHANEDIOL × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM P107S-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.63 Å R-free 0.180
3SLE Crystal Structure of the P107C-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-06-24	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）片段：UNP residues 32-417 链 F 33–417（385 aa）片段：UNP residues 32-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 ACT ACETATE ION × 4 EDO 1,2-ETHANEDIOL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP	分辨率 2.52 Å R-free 0.243
3SVW Crystal Structure of the P107V-MauG/pre-Methylamine Dehydrogenase Complex 提交 2011-07-12	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）片段：UNP residues 32-417 链 F 32–417（386 aa）片段：UNP residues 32-417	未记录	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 EDO 1,2-ETHANEDIOL × 3 ACT ACETATE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM P107V-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 23% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.86 Å R-free 0.174
3SWS Crystal Structure of the Quinone Form of Methylamine Dehydrogenase in Complex with the Diferric Form of MauG 提交 2011-07-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）片段：UNP residues 32-417 链 F 32–417（386 aa）片段：UNP residues 32-417	未记录	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 ACT ACETATE ION × 1 1PE PENTAETHYLENE GLYCOL × 1 PG4 TETRAETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM WT-MauG and 50uM MADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 25% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.2., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.86 Å R-free 0.178
3SXT Crystal Structure of the Quinol Form of Methylamine Dehydrogenase in Complex with the Diferrous Form of MauG 提交 2011-07-15	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 32–417（386 aa）片段：UNP residues 32-417 链 F 32–417（386 aa）片段：UNP residues 32-417	未记录	CA CALCIUM ION × 2 NA SODIUM ION × 4 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 5	X-RAY DIFFRACTION X-ray结晶条件 pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. WT-MauG and MADH were each reduced in an anaerobic glove box prior to preparing the protein mixture for crystallization. Protein mixture: 100uM reduced WT-MauG and 50uM reduced MADH in 10mM potassium phosphate pH7.5 with 2mM sodium dithionite. Reservoir solution contained: 22% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4 and 2mM sodium dithionite. Crystallization was carried out in an anaerobic glove box at ambient temperature., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.81 Å R-free 0.187
4FA1 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 130 Days. 提交 2012-05-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）链 F 33–417（385 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 2 EDO 1,2-ETHANEDIOL × 2 ACT ACETATE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.18 Å R-free 0.227
4FA5 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 20 Days 提交 2012-05-21	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）片段：unp residues 33-417 链 F 33–417（385 aa）片段：unp residues 33-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 NA SODIUM ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.94 Å R-free 0.204
4FA9 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 30 Days 提交 2012-05-21	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）片段：unp residues 33-417 链 F 33–417（385 aa）片段：unp residues 33-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 ACT ACETATE ION × 5 NA SODIUM ION × 4 EDO 1,2-ETHANEDIOL × 1 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.09 Å R-free 0.214
4FAN Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 40 Days 提交 2012-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）片段：unp residues 33-417 链 F 33–417（385 aa）片段：unp residues 33-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 ACT ACETATE ION × 1 NA SODIUM ION × 2 EDO 1,2-ETHANEDIOL × 1 PGE TRIETHYLENE GLYCOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.08 Å R-free 0.234
4FAV Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 50 Days 提交 2012-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）片段：unp residues 33-417 链 F 33–417（385 aa）片段：unp residues 33-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 2 EDO 1,2-ETHANEDIOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.08 Å R-free 0.224
4FB1 Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 60 Days 提交 2012-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）片段：unp residues 33-417 链 F 33–417（385 aa）片段：unp residues 33-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.15 Å R-free 0.222
4K3I Crystal Structure of the Quinol Form of Methylamine Dehydrogenase in Complex with the Diferrous Form of MauG, C2 Space Group 提交 2013-04-10	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）片段：UNP residues 33-417 链 F 33–417（385 aa）片段：UNP residues 33-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 4 EDO 1,2-ETHANEDIOL × 4 ACT ACETATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Drops contained 1uL protein with 3uL reservoir solution. WT-MauG and MADH were each reduced in an anaerobic glove box prior to preparing the protein mixture for crystallization. Protein mixture: 100uM reduced WT-MauG and 50uM reduced MADH in 10mM potassium phosphate pH7.5 with 2mM sodium dithionite. Reservoir solution contained: 24% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4 and 2mM sodium dithionite. Crystallization was carried out in an anaerobic glove box at ambient temperature., VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.00 Å R-free 0.190
4L1Q Crystal Structure of the E113Q-MauG/pre-Methylamine Dehydrogenase Complex 提交 2013-06-03	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）链 F 33–417（385 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 NA SODIUM ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30% w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.92 Å R-free 0.207
4L3G Crystal Structure of the E113Q-MauG/pre-Methylamine Dehydrogenase Complex Aged 120 Days 提交 2013-06-05	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）链 F 33–417（385 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 4 ACT ACETATE ION × 2 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30% w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.05 Å R-free 0.210
4L3H Crystal Structure of the E113Q-MauG/pre-Methylamine Dehydrogenase Complex After Treatment with Hydrogen Peroxide 提交 2013-06-05	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）链 F 33–417（385 aa）	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 NA SODIUM ION × 4 EDO 1,2-ETHANEDIOL × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 1.79 Å R-free 0.198
4O1Q Crystal Structure of the Q103N-MauG/pre-Methylamine Dehydrogenase Complex 提交 2013-12-16	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 33–417（385 aa）片段：UNP residues 33-417 链 F 33–417（385 aa）片段：UNP residues 33-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4 EDO 1,2-ETHANEDIOL × 3 PO4 PHOSPHATE ION × 1 PGE TRIETHYLENE GLYCOL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 NA SODIUM ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；0.1 M MES, pH 6.4, 0.1 M sodium acetate, 24-30% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.59 Å R-free 0.254
4Y5R Crystal Structure of a T67A MauG/pre-Methylamine Dehydrogenase Complex 提交 2015-02-11	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 D 42–417（376 aa）片段：UNP RESIDUES 42-417 链 F 42–417（376 aa）片段：UNP RESIDUES 42-417	未记录	CA CALCIUM ION × 2 HEC HEME C × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.4；292 K；25-30% PEG 8000, 0,1 M sodium Acetate, 0.1 M MES pH 6.4	分辨率 2.80 Å R-free 0.256

共 27 个其他 PDB 条目、27 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	A1BB97_PARDP
Isoform	—
PDB实体	3
链与序列区间	作者链 D； PDB构建体 1–385； UniProt 33–417 作者链 F； PDB构建体 1–385； UniProt 33–417

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	4fa4
沉积日期 deposition_date	2012-05-21
结构标题 title	Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 10 Days
关键词 keywords	Tryptophan tryptophylquinone, Oxidoreductase-Electron Transfer complex, Oxidoreductase-Electron transport complex; Oxidoreductase/Electron transport
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	42.53 Å
回转半径 Rg (电子) rg_electron	42.62 Å
零角强度 I(0) i0	1098120000.00
分子量 molecular_weight	179130.0 kDa
排除体积 excluded_volume	171990 Å³
包络体积 envelope_volume	296780 Å³
水化壳体积 shell_volume	60415 Å³
包络直径 envelope_diameter	174.4 Å
壳层 Rg shell_rg	45.16 Å
包络 Rg envelope_rg	43.10 Å
形状 Rg shape_rg	42.59 Å
总 Rg total_rg	42.75 Å
总原子数 total_atoms	13530
残基数 n_residues	1720
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	162.5 Å
Rg (实空间) rg_real	42.90 Å
Rg 误差 (实空间) rg_real_error	2.37 Å
I(0) (实空间) i0_real	1.0980e+09
I(0) 误差 (实空间) i0_real_error	2.4000e+07
Rg (倒空间) rg_reciprocal	42.54 Å
I(0) (倒空间) i0_reciprocal	1098000000.0000
解质量估计 total_estimate	0.8023
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	39.5 Å
偏度 Skewness skewness	0.634
峰度 Kurtosis kurtosis	0.165
角度范围 angular_range	— – 0.1850 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	102500000.0000
实空间数据点数 n_real_points	38
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.575; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.762; Smooth: 0.939

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (11)

实体 1 Entity 1 polymer

描述 description	Methylamine utilization protein MauG
分子量 formula_weight	41146.6 kDa
EC 编号 ec_number	1.-.-.-
来源方法 src_method	man
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B
原子数 atom_count	14775

EQARPADDALAALGAQLFVDPALSRNATQSCATCHDPARAFTDPREGKAGLAVSVGDDGQSHGDRNTPTLGYAALVPAFHRDANGKYKGGQFWDGRADDLKQQAGQPMLNPVEMAMPDRAAVAARLRDDPAYRTGFEALFGKGVLDDPERAFDAAAEALAAYQATGEFSPFDSKYDRVMRGEEKFTPLEEFGYTVFITWNCRLCHMQRKQGVAERETFTNFEYHNIGLPVNETAREASGLGADHVDHGLLARPGIEDPAQSGRFKVPSLRNVAVTGPYMHNGVFTDLRTAILFYNKYTSRRPEAKINPETGAPWGEPEVARNLSLAELQSGLMLDDGRVDALVAFLETLTDRRYEPLLEESRAAQKDHHHHHH

来源 Source

来源生物 organism	Paracoccus denitrificans
NCBI 分类编号 ncbi_taxonomy_id	318586
宿主生物 host_organism	Paracoccus denitrificans

实体 2 Entity 2 polymer

描述 description	Methylamine dehydrogenase light chain
分子量 formula_weight	15025.6 kDa
EC 编号 ec_number	1.4.9.1
来源方法 src_method	man
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	C,E

ADAPAGTDPRAKWVPQDNDIQACDYWRHCSIDGNICDCSGGSLTNCPPGTKLATASWVASCYNPTDGQSYLIAYRDCCGYNVSGRCPCLNTEGELPVYRPEFANDIIWCFGAEDDAMTYHCTISPIVGKASHHHHHH

来源 Source

来源生物 organism	Paracoccus denitrificans
NCBI 分类编号 ncbi_taxonomy_id	266
宿主生物 host_organism	Rhodobacter sphaeroides

实体 3 Entity 3 polymer

描述 description	Methylamine dehydrogenase heavy chain
分子量 formula_weight	42321.2 kDa
EC 编号 ec_number	1.4.99.3
来源方法 src_method	man
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	D,F

DAPEAETQAQETQGQAAARAAAADLAAGQDDEPRILEAPAPDARRVYVNDPAHFAAVTQQFVIDGEAGRVIGMIDGGFLPNPVVADDGSFIAHASTVFSRIARGERTDYVEVFDPVTLLPTADIELPDAPRFLVGTYPWMTSLTPDGKTLLFYQFSPAPAVGVVDLEGKAFKRMLDVPDCYHIFPTAPDTFFMHCRDGSLAKVAFGTEGTPEITHTEVFHPEDEFLINHPAYSQKAGRLVWPTYTGKIHQIDLSSGDAKFLPAVEALTEAERADGWRPGGWQQVAYHRALDRIYLLVDQRDEWRHKTASRFVVVLDAKTGERLAKFEMGHEIDSINVSQDEKPLLYALSTGDKTLYIHDAESGEELRSVNQLGHGPQVITTADMG

来源 Source

来源生物 organism	Paracoccus denitrificans
NCBI 分类编号 ncbi_taxonomy_id	318586
宿主生物 host_organism	Rhodobacter sphaeroides

实体 4 Entity 4 non-polymer

描述 description	CALCIUM ION
分子量 formula_weight	40.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 5 Entity 5 non-polymer

描述 description	HEME C
分子量 formula_weight	618.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

实体 6 Entity 6 non-polymer

描述 description	TRIETHYLENE GLYCOL
分子量 formula_weight	150.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

实体 7 Entity 7 non-polymer

描述 description	1,2-ETHANEDIOL
分子量 formula_weight	62.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 8 Entity 8 non-polymer

描述 description	SODIUM ION
分子量 formula_weight	23.0 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

实体 9 Entity 9 non-polymer

描述 description	PHOSPHATE ION
分子量 formula_weight	95.0 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

实体 10 Entity 10 non-polymer

描述 description	ACETATE ION
分子量 formula_weight	59.0 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 11 Entity 11 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1062

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7. 折叠分类 (SCOP + CATH) 11 domains

SCOP 2.08 (3 domains)

结构域编号 domain_id	d4fa4c1
类 Class class	g — Small proteins
折叠类型 Fold fold	g.21 — Methylamine dehydrogenase, L chain
超家族 Superfamily superfamily	g.21.1 — Methylamine dehydrogenase, L chain
家族 Family family	g.21.1.1 — Methylamine dehydrogenase, L chain

结构域编号 domain_id	d4fa4c2
类 Class class	l — Artifacts
折叠类型 Fold fold	l.1 — Tags
超家族 Superfamily superfamily	l.1.1 — Tags
家族 Family family	l.1.1.1 — Tags

结构域编号 domain_id	d4fa4e_
类 Class class	g — Small proteins
折叠类型 Fold fold	g.21 — Methylamine dehydrogenase, L chain
超家族 Superfamily superfamily	g.21.1 — Methylamine dehydrogenase, L chain
家族 Family family	g.21.1.1 — Methylamine dehydrogenase, L chain

CATH v4.4 (8 domains)

结构域编号 domain_id	4fa4A01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	760 — Cytochrome Bc1 Complex; Chain D, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cytochrome c-like domain

结构域编号 domain_id	4fa4A02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	760 — Cytochrome Bc1 Complex; Chain D, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cytochrome c-like domain

结构域编号 domain_id	4fa4B01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	760 — Cytochrome Bc1 Complex; Chain D, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cytochrome c-like domain

结构域编号 domain_id	4fa4B02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	760 — Cytochrome Bc1 Complex; Chain D, domain 2
同源超家族 H-superfamily homologous superfamily	10 — Cytochrome c-like domain

结构域编号 domain_id	4fa4C00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	30 — Electron Transport Ethylamine Dehydrogenase
同源超家族 H-superfamily homologous superfamily	10 — Methylamine/Aralkylamine dehydrogenase light chain

结构域编号 domain_id	4fa4D00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	130 — 7 Propeller
拓扑 Topology topology	10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

结构域编号 domain_id	4fa4E00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	30 — Electron Transport Ethylamine Dehydrogenase
同源超家族 H-superfamily homologous superfamily	10 — Methylamine/Aralkylamine dehydrogenase light chain

结构域编号 domain_id	4fa4F00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	130 — 7 Propeller
拓扑 Topology topology	10 — Methylamine Dehydrogenase; Chain H
同源超家族 H-superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

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8. 引用文献 (1)

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晶胞边长 a length_a	55.530 Å
晶胞边长 b length_b	83.520 Å
晶胞边长 c length_c	107.780 Å
晶胞夹角 α angle_alpha	109.92 °
晶胞夹角 β angle_beta	91.52 °
晶胞夹角 γ angle_gamma	105.78 °
晶胞分子数 Z z_pdb	2

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.140 Å
最低分辨率 resolution_low	43.460 Å
R 因子 r_factor	0.1664
R_work r_work	0.1632
R_free r_free	0.2268

fit	/app/data/saxs_computation/fa/4fa4/4fa4.fit
pddf	/app/data/pr_computation/fa/4fa4/4fa4.pr.out
profile	/app/data/saxs_computation/fa/4fa4/4fa4.dat

Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 10 Days

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Methylamine utilization protein MauG

当前结构中的状态

数据库中的同蛋白其他状态

Methylamine dehydrogenase light chain

当前结构中的状态

数据库中的同蛋白其他状态

Methylamine dehydrogenase heavy chain

当前结构中的状态

数据库中的同蛋白其他状态

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

2. 结构基本信息 2. Structure Basics

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

5. 晶体学与实验 5. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

6. 实体与聚合物信息 Entities & Polymers (11)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

来源 Source

实体 3 Entity 3 polymer

聚合物 Polymer

来源 Source

实体 4 Entity 4 non-polymer

实体 5 Entity 5 non-polymer

实体 6 Entity 6 non-polymer

实体 7 Entity 7 non-polymer

实体 8 Entity 8 non-polymer

实体 9 Entity 9 non-polymer

实体 10 Entity 10 non-polymer

实体 11 Entity 11 water

7. 折叠分类 (SCOP + CATH) 11 domains

SCOP 2.08 (3 domains)

CATH v4.4 (8 domains)

8. 引用文献 (1)

9. 文件与曲线 (10)

Files (7)

Curves (3)