4g0j

Crystallographic Analysis of Rotavirus NSP2-RNA Complex Reveals Specific Recognition of 5'-GG Sequence for RTPase activity

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Non-structural protein 2

Simian 11 rotavirus

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count
10	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count
2	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count
3	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count
4	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count
5	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count
6	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count
7	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count
8	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count
9	Protein monomer	Monomer	Protein 1	No other associated polymer	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NSP2_ROTSR
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–294； UniProt 1–294 Author chain B； PDBConstruct 1–294； UniProt 1–294 Author chain C； PDBConstruct 1–294； UniProt 1–294 Author chain D； PDBConstruct 1–294； UniProt 1–294 Author chain E； PDBConstruct 1–294； UniProt 1–294 Author chain F； PDBConstruct 1–294； UniProt 1–294 Author chain G； PDBConstruct 1–294； UniProt 1–294 Author chain H； PDBConstruct 1–294； UniProt 1–294 Author chain I； PDBConstruct 1–294； UniProt 1–294 Author chain J； PDBConstruct 1–294； UniProt 1–294

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	4g0j
Deposition date deposition_date	2012-07-09
Structure title title	Crystallographic Analysis of Rotavirus NSP2-RNA Complex Reveals Specific Recognition of 5'-GG Sequence for RTPase activity
Keywords keywords	RNA triphosphatase, RNA binding, hydrolase activity, nucleotide binding, metal ion binding, host cell cytoplasm, HYDROLASE; HYDROLASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

4g0j__assembly_9__model_1

Assembly 9 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

4g0j__assembly_9__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

4g0j__assembly_9__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	21.60 Å
Rg (electron density)	20.71 Å
Total Rg	21.63 Å
Atom count	2360
Residues	290
Excluded volume	42387 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`4g0j__assembly_1__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`4g0j__assembly_2__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`4g0j__assembly_3__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
4	1	`4g0j__assembly_4__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
5	1	`4g0j__assembly_5__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
6	1	`4g0j__assembly_6__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
7	1	`4g0j__assembly_7__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
8	1	`4g0j__assembly_8__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
9	1	`4g0j__assembly_9__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download
10	1	`4g0j__assembly_10__model_1`	monomeric （1）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (1)

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6. Fold Classification (SCOP + CATH) 20 domains

CATH v4.4 (20 domains)

Domain ID domain_id	4g0jA01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jA02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

Domain ID domain_id	4g0jB01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jB02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

Domain ID domain_id	4g0jC01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jC02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

Domain ID domain_id	4g0jD01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jD02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

Domain ID domain_id	4g0jE01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jE02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

Domain ID domain_id	4g0jF01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jF02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

Domain ID domain_id	4g0jG01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jG02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

Domain ID domain_id	4g0jH01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jH02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

Domain ID domain_id	4g0jI01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jI02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

Domain ID domain_id	4g0jJ01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1400 — Rotavirus NSP2 fragment, N-terminal domain
Homologous superfamily homologous superfamily	10 — Rotavirus NSP2 fragment, N-terminal domain

Domain ID domain_id	4g0jJ02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	428 — HIT family, subunit A
Homologous superfamily homologous superfamily	20 — Rotavirus NSP2 fragment, C-terminal domain

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Unit-cell length a length_a	122.484 Å
Unit-cell length b length_b	122.484 Å
Unit-cell length c length_c	301.127 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	40

Space group space_group	P 4
Space-group number int_tables_number	75
Crystal system crystal_system	—

Resolution resolution	3.398 Å
Low-resolution limit resolution_low	301.310 Å
R factor r_factor	0.2399
R_work r_work	0.2369
R_free r_free	0.2938

Description description	Non-structural protein 2
Molecular weight formula_weight	34070.4 kDa
EC 编号 ec_number	3.6.4.-
Mutation mutation	C-terminal deletion mutation
Source method src_method	man
Copy count entity_count	10

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H,I,J
Atom count atom_count	23600

Crystallographic Analysis of Rotavirus NSP2-RNA Complex Reveals Specific Recognition of 5'-GG Sequence for RTPase activity

1. Protein Identity and Related Structures Protein Identity & Related Structures

Non-structural protein 2

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4g0j__assembly_9__model_1

4g0j__assembly_9__model_1 | I(q)

4g0j__assembly_9__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

6. Fold Classification (SCOP + CATH) 20 domains

CATH v4.4 (20 domains)

7. Citations (1)

Source organism organism	Simian 11 rotavirus
Common name common_name	RV-A
NCBI taxonomy ID ncbi_taxonomy_id	36435
Host organism host_organism	Escherichia coli

Crystallographic Analysis of Rotavirus NSP2-RNA Complex Reveals Specific Recognition of 5'-GG Sequence for RTPase activity

1. Protein Identity and Related Structures Protein Identity & Related Structures

Non-structural protein 2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4g0j__assembly_9__model_1

4g0j__assembly_9__model_1 | I(q)

4g0j__assembly_9__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

6. Fold Classification (SCOP + CATH) 20 domains

CATH v4.4 (20 domains)

7. Citations (1)