SAXSdb — 4nwr

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1. Protein Identity and Related Structures Protein Identity & Related Structures

integron gene cassette protein

uncultured bacterium

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 24	Macrophage migration inhibitory factor-like protein × 12 （Q4Q413）	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 24	Macrophage migration inhibitory factor-like protein × 12 （Q4Q413）	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 24	Macrophage migration inhibitory factor-like protein × 12 （Q4Q413）	Consistent with protein count
4	Protein heterocomplex	Heteromer	Protein 24	Macrophage migration inhibitory factor-like protein × 12 （Q4Q413）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	B0BGB0_9BACT
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 0； PDBConstruct 1–158； UniProt 1–158 Author chain 2； PDBConstruct 1–158； UniProt 1–158 Author chain 4； PDBConstruct 1–158； UniProt 1–158 Author chain 6； PDBConstruct 1–158； UniProt 1–158 Author chain 8； PDBConstruct 1–158； UniProt 1–158 Author chain A； PDBConstruct 1–158； UniProt 1–158 Author chain AA； PDBConstruct 1–158； UniProt 1–158 Author chain AC； PDBConstruct 1–158； UniProt 1–158 Author chain AE； PDBConstruct 1–158； UniProt 1–158 Author chain AG； PDBConstruct 1–158； UniProt 1–158 Author chain AI； PDBConstruct 1–158； UniProt 1–158 Author chain AK； PDBConstruct 1–158； UniProt 1–158 Author chain AM； PDBConstruct 1–158； UniProt 1–158 Author chain AO； PDBConstruct 1–158； UniProt 1–158 Author chain AQ； PDBConstruct 1–158； UniProt 1–158 Author chain AS； PDBConstruct 1–158； UniProt 1–158 Author chain AU； PDBConstruct 1–158； UniProt 1–158 Author chain AW； PDBConstruct 1–158； UniProt 1–158 Author chain AY； PDBConstruct 1–158； UniProt 1–158 Author chain BA； PDBConstruct 1–158； UniProt 1–158 Author chain BC； PDBConstruct 1–158； UniProt 1–158 Author chain BE； PDBConstruct 1–158； UniProt 1–158 Author chain BG； PDBConstruct 1–158； UniProt 1–158 Author chain C； PDBConstruct 1–158； UniProt 1–158 Author chain E； PDBConstruct 1–158； UniProt 1–158 Author chain G； PDBConstruct 1–158； UniProt 1–158 Author chain I； PDBConstruct 1–158； UniProt 1–158 Author chain K； PDBConstruct 1–158； UniProt 1–158 Author chain M； PDBConstruct 1–158； UniProt 1–158 Author chain O； PDBConstruct 1–158； UniProt 1–158 Author chain Q； PDBConstruct 1–158； UniProt 1–158 Author chain S； PDBConstruct 1–158； UniProt 1–158 Author chain U； PDBConstruct 1–158； UniProt 1–158 Author chain W； PDBConstruct 1–158； UniProt 1–158 Author chain Y； PDBConstruct 1–158； UniProt 1–158 Author chain a； PDBConstruct 1–158； UniProt 1–158 Author chain c； PDBConstruct 1–158； UniProt 1–158 Author chain e； PDBConstruct 1–158； UniProt 1–158 Author chain g； PDBConstruct 1–158； UniProt 1–158 Author chain i； PDBConstruct 1–158； UniProt 1–158 Author chain k； PDBConstruct 1–158； UniProt 1–158 Author chain m； PDBConstruct 1–158； UniProt 1–158 Author chain o； PDBConstruct 1–158； UniProt 1–158 Author chain q； PDBConstruct 1–158； UniProt 1–158 Author chain s； PDBConstruct 1–158； UniProt 1–158 Author chain u； PDBConstruct 1–158； UniProt 1–158 Author chain w； PDBConstruct 1–158； UniProt 1–158 Author chain y； PDBConstruct 1–158； UniProt 1–158

Macrophage migration inhibitory factor-like protein

Leishmania major

UniProt Q4Q413

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 24	integron gene cassette protein × 12 （B0BGB0）	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 24	integron gene cassette protein × 12 （B0BGB0）	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 24	integron gene cassette protein × 12 （B0BGB0）	Consistent with protein count
4	Protein heterocomplex	Heteromer	Protein 24	integron gene cassette protein × 12 （B0BGB0）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q4Q413_LEIMA
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain 1； PDBConstruct 1–113； UniProt 1–113 Author chain 3； PDBConstruct 1–113； UniProt 1–113 Author chain 5； PDBConstruct 1–113； UniProt 1–113 Author chain 7； PDBConstruct 1–113； UniProt 1–113 Author chain 9； PDBConstruct 1–113； UniProt 1–113 Author chain AB； PDBConstruct 1–113； UniProt 1–113 Author chain AD； PDBConstruct 1–113； UniProt 1–113 Author chain AF； PDBConstruct 1–113； UniProt 1–113 Author chain AH； PDBConstruct 1–113； UniProt 1–113 Author chain AJ； PDBConstruct 1–113； UniProt 1–113 Author chain AL； PDBConstruct 1–113； UniProt 1–113 Author chain AN； PDBConstruct 1–113； UniProt 1–113 Author chain AP； PDBConstruct 1–113； UniProt 1–113 Author chain AR； PDBConstruct 1–113； UniProt 1–113 Author chain AT； PDBConstruct 1–113； UniProt 1–113 Author chain AV； PDBConstruct 1–113； UniProt 1–113 Author chain AX； PDBConstruct 1–113； UniProt 1–113 Author chain AZ； PDBConstruct 1–113； UniProt 1–113 Author chain B； PDBConstruct 1–113； UniProt 1–113 Author chain BB； PDBConstruct 1–113； UniProt 1–113 Author chain BD； PDBConstruct 1–113； UniProt 1–113 Author chain BF； PDBConstruct 1–113； UniProt 1–113 Author chain BH； PDBConstruct 1–113； UniProt 1–113 Author chain D； PDBConstruct 1–113； UniProt 1–113 Author chain F； PDBConstruct 1–113； UniProt 1–113 Author chain H； PDBConstruct 1–113； UniProt 1–113 Author chain J； PDBConstruct 1–113； UniProt 1–113 Author chain L； PDBConstruct 1–113； UniProt 1–113 Author chain N； PDBConstruct 1–113； UniProt 1–113 Author chain P； PDBConstruct 1–113； UniProt 1–113 Author chain R； PDBConstruct 1–113； UniProt 1–113 Author chain T； PDBConstruct 1–113； UniProt 1–113 Author chain V； PDBConstruct 1–113； UniProt 1–113 Author chain X； PDBConstruct 1–113； UniProt 1–113 Author chain Z； PDBConstruct 1–113； UniProt 1–113 Author chain b； PDBConstruct 1–113； UniProt 1–113 Author chain d； PDBConstruct 1–113； UniProt 1–113 Author chain f； PDBConstruct 1–113； UniProt 1–113 Author chain h； PDBConstruct 1–113； UniProt 1–113 Author chain j； PDBConstruct 1–113； UniProt 1–113 Author chain l； PDBConstruct 1–113； UniProt 1–113 Author chain n； PDBConstruct 1–113； UniProt 1–113 Author chain p； PDBConstruct 1–113； UniProt 1–113 Author chain r； PDBConstruct 1–113； UniProt 1–113 Author chain t； PDBConstruct 1–113； UniProt 1–113 Author chain v； PDBConstruct 1–113； UniProt 1–113 Author chain x； PDBConstruct 1–113； UniProt 1–113 Author chain z； PDBConstruct 1–113； UniProt 1–113

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	4nwr
Deposition date deposition_date	2013-12-06
Structure title title	Computationally Designed Two-Component Self-Assembling Tetrahedral Cage T33-28
Keywords keywords	;two-component, self-assembling, tetrahedron, designed protein cage, computational design, computational biology, protein engineering, multimerization, nanomaterial, nanostructure, integrons, macrophage migration-inhibitory factors, PROTEIN BINDING ;; PROTEIN BINDING
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

4nwr__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

4nwr__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

4nwr__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	49.72 Å
Rg (electron density)	48.76 Å
Total Rg	48.94 Å
Atom count	22245
Residues	3173
Excluded volume	391430 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`4nwr__assembly_1__model_1`	24-meric （24）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`4nwr__assembly_2__model_1`	24-meric （24）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`4nwr__assembly_3__model_1`	24-meric （24）	Success	4.1.3-1-20251215 (887e7ef)	View Download
4	1	`4nwr__assembly_4__model_1`	24-meric （24）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Description description	integron gene cassette protein
Molecular weight formula_weight	17789.0 kDa
Mutation mutation	K49I, D53A, Q56I, S57F, I58L, F64D, L109E, E112L, K113A, A116V
Source method src_method	man
Copy count entity_count	48

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,C,E,G,I,K,M,O,Q,S,U,W,Y,a,c,e,g,i,k,m,o,q,s,u,w,y,0,2,4,6,8,AA,AC,AE,AG,AI,AK,AM,AO,AQ,AS,AU,AW,AY,BA,BC,BE,BG
Atom count atom_count	88858

MESVNTSFLSPSLVTIRDFDNGQFAVLRIGRTGFPADKGDIDLCLDKMIGVRAAQIFLGDDTEDGFKGPHIRIRCVDIDDKHTYNAMVYVDLIVGTGASEVERETAEEEAKLALRVALQVDIADEHSCVTQFEMKLREELLSSDSFHPDKDEYYKDFL

Source Source

Source organism organism	uncultured bacterium
NCBI taxonomy ID ncbi_taxonomy_id	77133
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	Macrophage migration inhibitory factor-like protein
Molecular weight formula_weight	13517.7 kDa
Mutation mutation	E18L, N19L, Q22I, V23I, A26I, D30V, K87A, E88V
Source method src_method	man
Copy count entity_count	48

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,D,F,H,J,L,N,P,R,T,V,X,Z,b,d,f,h,j,l,n,p,r,t,v,x,z,1,3,5,7,9,AB,AD,AF,AH,AJ,AL,AN,AP,AR,AT,AV,AX,AZ,BB,BD,BF,BH

MPVIQTFVSTPLDHHKRLLLAIIYRIVTRVVLGKPEDLVMMTFHDSTPMHFFGSTDPVACVRVEALGGYGPSEPEKVTSIVTAAITAVCGIVADRIFVLYFSPLHCGWNGTNFLEHHHHHH

Source Source

Source organism organism	Leishmania major
NCBI taxonomy ID ncbi_taxonomy_id	5664
Host organism host_organism	Escherichia coli

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Unit-cell length a length_a	124.910 Å
Unit-cell length b length_b	189.250 Å
Unit-cell length c length_c	376.830 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.02 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	96

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	3.500 Å
Low-resolution limit resolution_low	94.207 Å
R factor r_factor	0.2653
R_work r_work	0.2616
R_free r_free	0.2983

Computationally Designed Two-Component Self-Assembling Tetrahedral Cage T33-28

1. Protein Identity and Related Structures Protein Identity & Related Structures

integron gene cassette protein

State in the Current Structure

Other States of the Same Protein in the Database

Macrophage migration inhibitory factor-like protein

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4nwr__assembly_1__model_1

4nwr__assembly_1__model_1 | I(q)

4nwr__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

7. Citations (1)

Computationally Designed Two-Component Self-Assembling Tetrahedral Cage T33-28

1. Protein Identity and Related Structures Protein Identity & Related Structures

integron gene cassette protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Macrophage migration inhibitory factor-like protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4nwr__assembly_1__model_1

4nwr__assembly_1__model_1 | I(q)

4nwr__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

7. Citations (1)