4oq9

Satellite Tobacco Mosaic Virus Refined to 1.4 A Resolution using non-crystallographic symmetry restraints

Method: X-RAY DIFFRACTION Dmax: 148.7 Å Quality: GOOD

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Coat protein

物种未注明

UniProt P17574

当前结构中的状态

Assembly 聚集状态 构建体 突变与修饰 配体、离子与共同组分 实验方法与环境 结构质量
1 蛋白–RNA 同源多聚体 蛋白 × 60 RNA 180 PDB 声明:240-meric(240) 与全部聚合物数一致 链 A; UniProt 1–159 链 B; UniProt 1–159 链 C; UniProt 1–159 链 D; UniProt 1–159 链 E; UniProt 1–159 链 F; UniProt 1–159 链 G; UniProt 1–159 链 H; UniProt 1–159 链 I; UniProt 1–159 链 J; UniProt 1–159 链 K; UniProt 1–159 链 L; UniProt 1–159 链 M; UniProt 1–159 链 N; UniProt 1–159 链 O; UniProt 1–159 未记录 ;RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') ; × 60 ;RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') ; × 60 ;RNA (5'-R(P*UP*U)-3') ; × 60 PO4 PHOSPHATE ION × 12 SO4 SULFATE ION × 72 NA SODIUM ION × 68 MG MAGNESIUM ION × 12 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 6.5;296 K;crystals grown from 15% ammonium sulfate on earth with a reservoir of 15% ammonium sulfate over 14 days., pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K 分辨率 1.45 Å R-free 0.250
2 蛋白–RNA 同源多聚体 蛋白 × 15 RNA 45 PDB 声明:60-meric(60) 与全部聚合物数一致 链 A; UniProt 1–159 链 B; UniProt 1–159 链 C; UniProt 1–159 链 D; UniProt 1–159 链 E; UniProt 1–159 链 F; UniProt 1–159 链 G; UniProt 1–159 链 H; UniProt 1–159 链 I; UniProt 1–159 链 J; UniProt 1–159 链 K; UniProt 1–159 链 L; UniProt 1–159 链 M; UniProt 1–159 链 N; UniProt 1–159 链 O; UniProt 1–159 未记录 ;RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') ; × 15 ;RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') ; × 15 ;RNA (5'-R(P*UP*U)-3') ; × 15 PO4 PHOSPHATE ION × 3 SO4 SULFATE ION × 18 NA SODIUM ION × 17 MG MAGNESIUM ION × 3 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 6.5;296 K;crystals grown from 15% ammonium sulfate on earth with a reservoir of 15% ammonium sulfate over 14 days., pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K 分辨率 1.45 Å R-free 0.250
3 蛋白–RNA 同源多聚体 蛋白 × 15 RNA 45 PDB 声明:60-meric(60) 与全部聚合物数一致 链 A; UniProt 1–159 链 B; UniProt 1–159 链 C; UniProt 1–159 链 D; UniProt 1–159 链 E; UniProt 1–159 链 F; UniProt 1–159 链 G; UniProt 1–159 链 H; UniProt 1–159 链 I; UniProt 1–159 链 J; UniProt 1–159 链 K; UniProt 1–159 链 L; UniProt 1–159 链 M; UniProt 1–159 链 N; UniProt 1–159 链 O; UniProt 1–159 未记录 ;RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') ; × 15 ;RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') ; × 15 ;RNA (5'-R(P*UP*U)-3') ; × 15 PO4 PHOSPHATE ION × 3 SO4 SULFATE ION × 18 NA SODIUM ION × 17 MG MAGNESIUM ION × 3 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 6.5;296 K;crystals grown from 15% ammonium sulfate on earth with a reservoir of 15% ammonium sulfate over 14 days., pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K 分辨率 1.45 Å R-free 0.250
4 蛋白–RNA 同源多聚体 蛋白 × 15 RNA 45 PDB 声明:60-meric(60) 与全部聚合物数一致 链 A; UniProt 1–159 链 B; UniProt 1–159 链 C; UniProt 1–159 链 D; UniProt 1–159 链 E; UniProt 1–159 链 F; UniProt 1–159 链 G; UniProt 1–159 链 H; UniProt 1–159 链 I; UniProt 1–159 链 J; UniProt 1–159 链 K; UniProt 1–159 链 L; UniProt 1–159 链 M; UniProt 1–159 链 N; UniProt 1–159 链 O; UniProt 1–159 未记录 ;RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') ; × 15 ;RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') ; × 15 ;RNA (5'-R(P*UP*U)-3') ; × 15 PO4 PHOSPHATE ION × 3 SO4 SULFATE ION × 18 NA SODIUM ION × 17 MG MAGNESIUM ION × 3 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 6.5;296 K;crystals grown from 15% ammonium sulfate on earth with a reservoir of 15% ammonium sulfate over 14 days., pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K 分辨率 1.45 Å R-free 0.250

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly, “相对当前条目”直接指出证据层面的不同;没有差异标签表示当前已读取字段一致。

共 13 个其他 PDB 条目、23 个 assembly。 打开独立比较页并筛选聚集状态

查看构建体与数据证据
UniProt名称 COAT_STMV
Isoform
PDB实体 1
链与序列区间 作者链 A; PDB构建体 1–159; UniProt 1–159 作者链 B; PDB构建体 1–159; UniProt 1–159 作者链 C; PDB构建体 1–159; UniProt 1–159 作者链 D; PDB构建体 1–159; UniProt 1–159 作者链 E; PDB构建体 1–159; UniProt 1–159 作者链 F; PDB构建体 1–159; UniProt 1–159 作者链 G; PDB构建体 1–159; UniProt 1–159 作者链 H; PDB构建体 1–159; UniProt 1–159 作者链 I; PDB构建体 1–159; UniProt 1–159 作者链 J; PDB构建体 1–159; UniProt 1–159 作者链 K; PDB构建体 1–159; UniProt 1–159 作者链 L; PDB构建体 1–159; UniProt 1–159 作者链 M; PDB构建体 1–159; UniProt 1–159 作者链 N; PDB构建体 1–159; UniProt 1–159 作者链 O; PDB构建体 1–159; UniProt 1–159

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

SAXS profile for 4oq9

P(r) 距离分布 P(r) Distribution

P(r) distribution for 4oq9
下载 Download

2. 结构基本信息 2. Structure Basics

条目编号 entry_id4oq9
沉积日期 deposition_date2014-02-07
结构标题 titleSatellite Tobacco Mosaic Virus Refined to 1.4 A Resolution using non-crystallographic symmetry restraints
关键词 keywordsDouble-helix RNA, Protein-RNA complex, Swiss Jelly-roll beta barrel, VIRUS-RNA complex; VIRUS/RNA
实验方法 methodX-RAY DIFFRACTION

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier49.97
回转半径 Rg (电子) rg_electron50.61
零角强度 I(0) i01642440000.00
分子量 molecular_weight293160.0 kDa
排除体积 excluded_volume348210 ų
包络体积 envelope_volume608370 ų
水化壳体积 shell_volume95960 ų
包络直径 envelope_diameter149.5
壳层 Rg shell_rg58.01
包络 Rg envelope_rg49.69
形状 Rg shape_rg50.64
总 Rg total_rg50.74
总原子数 total_atoms20320
残基数 n_residues2328
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax148.7
Rg (实空间) rg_real49.84
Rg 误差 (实空间) rg_real_error1.14
I(0) (实空间) i0_real1.6420e+09
I(0) 误差 (实空间) i0_real_error2.7060e+07
Rg (倒空间) rg_reciprocal50.06
I(0) (倒空间) i0_reciprocal1643000000.0000
解质量估计 total_estimate0.8549
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary60.4
偏度 Skewness skewness0.130
峰度 Kurtosis kurtosis-0.722
角度范围 angular_range— – 0.1600 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha121800000.0000
实空间数据点数 n_real_points33
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.989; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.149

5. 晶体学与实验 5. Crystallography & Experiment

6. 实体与聚合物信息 Entities & Polymers (9)

7. 折叠分类 (SCOP + CATH) 30 domains

SCOP 2.08 (15 domains)

结构域编号 domain_idd4oq9a_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9b_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9c_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9d_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9e_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9f_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9g_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9h_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9i_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9j_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9k_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9l_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9m_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9n_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses
结构域编号 domain_idd4oq9o_
类 Class classb — All beta proteins
折叠类型 Fold foldb.121 — Nucleoplasmin-like/VP (viral coat and capsid proteins)
超家族 Superfamily superfamilyb.121.7 — Satellite viruses
家族 Family familyb.121.7.1 — Satellite viruses

CATH v4.4 (15 domains)

结构域编号 domain_id4oq9A00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9B00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9C00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9D00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9E00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9F00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9G00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9H00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9I00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9J00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9K00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9L00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9M00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9N00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain
结构域编号 domain_id4oq9O00
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily220 — Satellite virus coat domain

8. 引用文献 (8)

9. 文件与曲线 (10)