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5b5w

Crystal structure of MOB1-LATS1 NTR domain complex

Method: X-RAY DIFFRACTION Dmax: 78.7 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

MOB kinase activator 1B

Mus musculus

UniProt Q8BPB0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 33–216 Fragment：UNP residues 33-216 Serine/threonine-protein kinase LATS1 × 1 （O95835） ZN ZINC ION × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；PEG 4000 Resolution 2.96 Å R-free 0.270

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5B5W Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5B5V Structure of full-length MOB1b Deposited 2016-05-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–216（216 aa）
Chain C 1–216（216 aa）
Chain E 1–216（216 aa）
Chain F 1–216（216 aa）
Not recorded ZN ZINC ION × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, Ammonium fluoride
Resolution 2.19 Å R-free 0.246
5B5V Structure of full-length MOB1b Deposited 2016-05-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 1–216（216 aa）
Chain D 1–216（216 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, Ammonium fluoride
Resolution 2.19 Å R-free 0.246
5B6B Complex of LATS1 and phosphomimetic MOB1b Deposited 2016-05-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–216（216 aa）
Chain B 1–216（216 aa）
Mutation：T35D, T22D Mutation：T35D, T22D CL CHLORIDE ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, sodium sulfate
Resolution 3.54 Å R-free 0.266
5B6B Complex of LATS1 and phosphomimetic MOB1b Deposited 2016-05-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain D 1–216（216 aa）
Chain K 1–216（216 aa）
Mutation：T35D, T22D Mutation：T35D, T22D CL CHLORIDE ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, sodium sulfate
Resolution 3.54 Å R-free 0.266
5B6B Complex of LATS1 and phosphomimetic MOB1b Deposited 2016-05-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain F 1–216（216 aa）
Chain H 1–216（216 aa）
Mutation：T35D, T22D Mutation：T35D, T22D CL CHLORIDE ION × 2 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, sodium sulfate
Resolution 3.54 Å R-free 0.266
5B6B Complex of LATS1 and phosphomimetic MOB1b Deposited 2016-05-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain M 1–216（216 aa）
Chain O 1–216（216 aa）
Mutation：T35D, T22D Mutation：T35D, T22D CL CHLORIDE ION × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, sodium sulfate
Resolution 3.54 Å R-free 0.266

2 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MOB1B_MOUSE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 3–186； UniProt 33–216

Serine/threonine-protein kinase LATS1

Homo sapiens

UniProt O95835

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain U； UniProt 622–704 Fragment：UNP residues 622-704 MOB kinase activator 1B × 1 （Q8BPB0） ZN ZINC ION × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；277 K；PEG 4000 Resolution 2.96 Å R-free 0.270

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5B5W Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4ZRK Merlin-FERM and Lats1 complex Deposited 2015-05-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 69–100（32 aa） Fragment：UNP residues 69-100
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1 M Sodium malonate, 10% PEG 3350
Resolution 2.32 Å R-free 0.279
4ZRK Merlin-FERM and Lats1 complex Deposited 2015-05-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 69–100（32 aa） Fragment：UNP residues 69-100
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1 M Sodium malonate, 10% PEG 3350
Resolution 2.32 Å R-free 0.279
4ZRK Merlin-FERM and Lats1 complex Deposited 2015-05-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 69–100（32 aa） Fragment：UNP residues 69-100
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1 M Sodium malonate, 10% PEG 3350
Resolution 2.32 Å R-free 0.279
4ZRK Merlin-FERM and Lats1 complex Deposited 2015-05-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 69–100（32 aa） Fragment：UNP residues 69-100
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1 M Sodium malonate, 10% PEG 3350
Resolution 2.32 Å R-free 0.279
5BRK pMob1-Lats1 complex Deposited 2015-05-31 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 602–704（103 aa） Fragment：UNP residues 602-704
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.2 M di-Ammonium citrate 20% (W/V) PEG 3,350
Resolution 2.30 Å R-free 0.221
7LWH Human neurofibromin 2/merlin residues 1-339 in complex with LATS1 Deposited 2021-03-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 69–91（23 aa）
Not recorded IMD IMIDAZOLE × 3 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；600 mM imidazole (pH 6.5), 15% (w/v) polyethylene glycol 3,350
Resolution 1.61 Å R-free 0.233

3 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LATS1_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain U； PDBConstruct 3–85； UniProt 622–704

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 5b5w

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 5b5w
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2. Structure Basics 2. Structure Basics

Entry ID entry_id5b5w
Deposition date deposition_date2016-05-24
Structure title titleCrystal structure of MOB1-LATS1 NTR domain complex
Keywords keywordsMOB1 LATS1 Hippo pathway, METAL BINDING PROTEIN-APOTOSIS complex; METAL BINDING PROTEIN/APOTOSIS
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier21.14 Å
Radius of gyration Rg (electron density) rg_electron20.09 Å
Forward intensity I(0) i013684800.00
Molecular weight molecular_weight28005.0 kDa
Excluded volume excluded_volume35180 ų
Envelope volume envelope_volume42475 ų
Hydration-shell volume shell_volume18625 ų
Envelope diameter envelope_diameter77.0 Å
Shell Rg shell_rg25.69 Å
Envelope Rg envelope_rg20.66 Å
Shape Rg shape_rg20.00 Å
Total Rg total_rg21.21 Å
Total atoms total_atoms1966
Residues n_residues236
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax78.7 Å
Rg (real space) rg_real21.27 Å
Rg uncertainty (real space) rg_real_error0.72 Å
I(0) (real space) i0_real1.3680e+07
I(0) uncertainty (real space) i0_real_error2.0540e+05
Rg (reciprocal space) rg_reciprocal21.25 Å
I(0) (reciprocal space) i0_reciprocal13680000.0000
Solution quality estimate total_estimate0.8103
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary24.3 Å
Skewness Skewness skewness0.586
Kurtosis Kurtosis kurtosis0.169
Angular range angular_range— – 0.3750 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha2864000.0000
Real-space data points n_real_points69
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.580; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.791; Smooth: 0.997
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd5b5wa_
Class classa — All alpha proteins
Fold Fold folda.29 — Bromodomain-like
Superfamily Superfamily superfamilya.29.7 — Mob1/phocein
Family Family familya.29.7.1 — Mob1/phocein

CATH v4.4 (1 domains)

Domain ID domain_id5b5wA00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3
Homologous superfamily homologous superfamily30 — MOB kinase activator
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8. Citations (1)

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9. Files and Curves (10)