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5e8i

Crystal structure of the DNA binding domain of human transcription factor FLI1 in complex with a 10-mer DNA ACCGGAAGTG

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Friend leukemia integration 1 transcription factor

Homo sapiens

UniProt Q01543

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Homooligomer Protein 2 DNA 4 ;DNA (5'-D(*AP*CP*CP*GP*GP*AP*AP*GP*TP*G)-3') ; × 2 ;DNA (5'-D(*CP*AP*CP*TP*TP*CP*CP*GP*GP*T)-3') ; × 2 CALCIUM ION × 5 water × 2 Consistent with all polymers
2 Protein–DNA Homooligomer Protein 2 DNA 4 ;DNA (5'-D(*AP*CP*CP*GP*GP*AP*AP*GP*TP*G)-3') ; × 2 ;DNA (5'-D(*CP*AP*CP*TP*TP*CP*CP*GP*GP*T)-3') ; × 2 CALCIUM ION × 3 water × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
1fli 1x66 2ytu

3 assemblies

Homodimer
5e8g 5e8g 9cp6

3 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
5e8i 5e8i 5jvt 5jvt 5jvt 6vg2 6vg2 6vg8 6vgd 9mwy 9mx8 9mx9

13 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FLI1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 5–128； UniProt 276–399 Author chain D； PDBConstruct 5–128； UniProt 276–399 Author chain G； PDBConstruct 5–128； UniProt 276–399 Author chain J； PDBConstruct 5–128； UniProt 276–399

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id5e8i
Deposition date deposition_date2015-10-14
Structure title titleCrystal structure of the DNA binding domain of human transcription factor FLI1 in complex with a 10-mer DNA ACCGGAAGTG
Keywords keywordsTranscription, DNA binding, Ewing sarcoma, Winged helix, ETS family, DNA BINDING PROTEIN-DNA complex; DNA BINDING PROTEIN/DNA
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5e8i__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5e8i__assembly_1__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

5e8i__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）24.26 Å
Rg (electron density)22.40 Å
Total Rg23.44 Å
Atom count2358
Residues226
Excluded volume39366 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 5e8i__assembly_1__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 5e8i__assembly_2__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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6. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

Domain ID domain_id5e8iA00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Domain ID domain_id5e8iD00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Domain ID domain_id5e8iG00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Domain ID domain_id5e8iJ00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
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7. Citations (1)