5heb

The third PDZ domain from the synaptic protein PSD-95 in complex with a C-terminal peptide derived from CRIPT

Method: X-RAY DIFFRACTION D_max: 58.8 Å Quality: GOOD

▼

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Disks large homolog 4

Rattus norvegicus

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 A； UniProt 302–402	片段：PDZ-3 domain (UNP residues 302-402)	Cysteine-rich PDZ-binding protein × 1 （Q792Q4） GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.65 Å R-free 0.195

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 5HEB	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1BE9 THE THIRD PDZ DOMAIN FROM THE SYNAPTIC PROTEIN PSD-95 IN COMPLEX WITH A C-TERMINAL PEPTIDE DERIVED FROM CRIPT. 提交 1998-05-20	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 295–420（126 aa）片段：THE THIRD PDZ DOMAIN OF PSD-95	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 7.5；0.8 M SODIUM CITRATE, 0.1 M HEPES, PH 7.5	分辨率 1.82 Å R-free 0.278
1BFE THE THIRD PDZ DOMAIN FROM THE SYNAPTIC PROTEIN PSD-95 提交 1998-05-20	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 302–402（101 aa）片段：THE THIRD PDZ DOMAIN OF PSD-95	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 7.5；0.8 M SODIUM CITRATE, 0.1 M HEPES, PH 7.5	分辨率 2.30 Å R-free 0.315
1IU0 The first PDZ domain of PSD-95 提交 2002-02-18	构建体不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 61–151（91 aa）片段：PDZ1 domain	未记录	未记录非水小分子	SOLUTION NMR NMR测量条件 pH 6.8；303 K；离子强度（mmCIF原始值）100mM KCl；压力 1 NMR样品组成 20mM KPi \| 10% D2O, 90% H2O NMR样品组成 20mM KPi \| 100% D2O	分辨率未提供
1IU2 The first PDZ domain of PSD-95 提交 2002-02-19	构建体不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 61–151（91 aa）片段：PDZ1 domain	未记录	未记录非水小分子	SOLUTION NMR NMR测量条件 pH 6.8；303 K；离子强度（mmCIF原始值）100mM KCl；压力 1 NMR样品组成 20mM KPi, 100mM KCl \| 10% D2O, 90% H2O NMR样品组成 20mM KPi, 100mM KCl \| 100% D2O	分辨率未提供
1JXM CRYSTAL STRUCTURE OF THE GMP BOUND SH3-HOOK-GK FRAGMENT OF PSD-95 提交 2001-09-07	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 430–724（295 aa）片段：SH3-HOOK-GK	未记录	5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GAI GUANIDINE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.7；277 K；2-methyl-2,4-pentanediol, HEPES buffer, guanidine, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K	分辨率 2.00 Å R-free 0.260
1JXO Crystal Structure of the SH3-HOOK-GK Fragment of PSD-95 提交 2001-09-07	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 430–724（295 aa）片段：SH3-HOOK-GK	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；2-methyl-2,4-pentanediol, MES buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.264
1JXO Crystal Structure of the SH3-HOOK-GK Fragment of PSD-95 提交 2001-09-07	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 430–724（295 aa）片段：SH3-HOOK-GK	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；2-methyl-2,4-pentanediol, MES buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.264
1KJW SH3-Guanylate Kinase Module from PSD-95 提交 2001-12-05	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 430–724（295 aa）片段：SH3 Domain/Guanylate Kinase Domain	未记录	SO4 SULFATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；288 K；Ammonium sulfate, PEG 4000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 15K	分辨率 1.80 Å R-free 0.250
1QLC Solution structure of the second PDZ domain of Postsynaptic Density-95 提交 1999-08-25	构建体不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 155–249（95 aa）片段：THE SECOND PDZ DOMAIN	未记录	未记录非水小分子	SOLUTION NMR NMR测量条件 pH 6；303 K；离子强度（mmCIF原始值）100；压力 1	分辨率未提供
1RGR Cyclic Peptides Targeting PDZ Domains of PSD-95: Structural Basis for Enhanced Affinity and Enzymatic Stability 提交 2003-11-12	构建体不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 62–154（93 aa）片段：PDZ1 domain of PSD-95	未记录	BAL BETA-ALANINE × 1	SOLUTION NMR NMR样品组成 1mM PZ1 U-15N,13C, 3mM peptide, 10 mM phospate buffer 150 mM NaCl, pH 6.8 \| 90% H2O/10% D2O	分辨率未提供
1TP3 PDZ3 domain of PSD-95 protein complexed with KKETPV peptide ligand 提交 2004-06-15	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：PDZ 3; residues 302-402	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.9；295 K；1.0 M sodium citrate, 0.1 M HEPES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K	分辨率 1.99 Å R-free 0.296
1TP5 Crystal structure of PDZ3 domain of PSD-95 protein complexed with a peptide ligand KKETWV 提交 2004-06-15	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：third PDZ domain (residues 302-402)	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.9；288 K；1.0 M sodium citrate, 0.1 M HEPES, 10 mM sodium chloride, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 288K	分辨率 1.54 Å R-free 0.229
1TP5 Crystal structure of PDZ3 domain of PSD-95 protein complexed with a peptide ligand KKETWV 提交 2004-06-15	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 A 302–402（101 aa）片段：third PDZ domain (residues 302-402)	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.9；288 K；1.0 M sodium citrate, 0.1 M HEPES, 10 mM sodium chloride, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 288K	分辨率 1.54 Å R-free 0.229
1TQ3 Higher resolution crystal structure of the third PDZ domain of post synaptic PSD-95 protein 提交 2004-06-16	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 302–402（101 aa）片段：third PDZ domain (residues 302-402)	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.9；295 K；1.0 M sodium citrate, 0.1 M HEPES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K	分辨率 1.89 Å R-free 0.296
2KA9 Solution structure of PSD-95 PDZ12 complexed with cypin peptide 提交 2008-11-03	构建体不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 A 61–249（189 aa）片段：N-terminal PDZ12 domain	未记录	未记录非水小分子	SOLUTION NMR NMR测量条件 pH 6.5；303 K；离子强度（mmCIF原始值）100；压力 ambient NMR样品组成 1mM [U-15N] PSD95 PDZ12, 1mM cypin peptide, 90% H2O, 10% D2O \| 90% H2O/10% D2O	分辨率未提供
2MHO Solution State Structure PSD-95 PDZ1 with 5HT2C Receptor peptide 提交 2013-11-29	构建体不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 60–155（96 aa）片段：PDZ 1, UNP residues 60-155	未记录	未记录非水小分子	SOLUTION NMR NMR测量条件 pH 6.3；298 K；离子强度（mmCIF原始值）20；压力 ambient NMR样品组成 0.5 mM [U-13C; U-15N] protein_1, 2.5 mM protein_2, 1 mM DTT, 0.01 w/v sodium azide, 20 mM sodium phosphate, 90% H2O/10% D2O	分辨率未提供
2XKX Single particle analysis of PSD-95 in negative stain 提交 2010-07-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 1–724（724 aa）链 B 1–724（724 aa）	突变：YES 突变：YES	未记录非水小分子	实验方法未声明 cryo-EM缓冲液 20MM TRIS/HCL, 5MM DTT, 1MM EDTA,；pH 7.5；20MM TRIS/HCL, 5MM DTT, 1MM EDTA,	分辨率 22.90 Å
3GSL Crystal structure of PSD-95 tandem PDZ domains 1 and 2 提交 2009-03-27	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 61–249（189 aa）片段：PDZ domains 1 and 2: UNP residues 61-249	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；35% v/v 2-methyl-2,4-pentanediol, 0.1M Tris-HCl pH 7.0. 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K	分辨率 2.05 Å R-free 0.281
3GSL Crystal structure of PSD-95 tandem PDZ domains 1 and 2 提交 2009-03-27	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 61–249（189 aa）片段：PDZ domains 1 and 2: UNP residues 61-249	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；35% v/v 2-methyl-2,4-pentanediol, 0.1M Tris-HCl pH 7.0. 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K	分辨率 2.05 Å R-free 0.281
3WP0 Crystal structure of Dlg GK in complex with a phosphor-Lgl2 peptide 提交 2014-01-08	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 531–713（183 aa）片段：UNP residues 533-713	未记录	GOL GLYCEROL × 7	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.8；291 K；0.2M lithium chloride, 20% PEG3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K	分辨率 2.04 Å R-free 0.202
3WP1 Phosphorylation-dependent interaction between tumor suppressors Dlg and Lgl 提交 2014-01-08	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 531–713（183 aa）片段：UNP residues 531-713	未记录	SO4 SULFATE ION × 4	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å R-free 0.268
5D13 Third PDZ domain (PDZ3) of PSD-95 complexed with CFMOC-KKETEV peptide 提交 2015-08-03	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：unp residues 302-402	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.2；295 K；20 mM HEPES 100 mM NaCl 1.8 M tri-sodium citrate	分辨率 2.15 Å R-free 0.257
5D13 Third PDZ domain (PDZ3) of PSD-95 complexed with CFMOC-KKETEV peptide 提交 2015-08-03	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 302–402（101 aa）片段：unp residues 302-402	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.2；295 K；20 mM HEPES 100 mM NaCl 1.8 M tri-sodium citrate	分辨率 2.15 Å R-free 0.257
5D13 Third PDZ domain (PDZ3) of PSD-95 complexed with CFMOC-KKETEV peptide 提交 2015-08-03	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 C 302–402（101 aa）片段：unp residues 302-402	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.2；295 K；20 mM HEPES 100 mM NaCl 1.8 M tri-sodium citrate	分辨率 2.15 Å R-free 0.257
5D13 Third PDZ domain (PDZ3) of PSD-95 complexed with CFMOC-KKETEV peptide 提交 2015-08-03	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 D 302–402（101 aa）片段：unp residues 302-402	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.2；295 K；20 mM HEPES 100 mM NaCl 1.8 M tri-sodium citrate	分辨率 2.15 Å R-free 0.257
5GNV Structure of PSD-95/MAP1A complex reveals unique target recognition mode of MAGUK GK domain 提交 2016-07-25	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 531–713（183 aa）片段：UNP residues 531-713	未记录	SO4 SULFATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件 EVAPORATION；pH 6.5；289 K；0.2M lithium sulfate, 0.1M Bis-Tris pH 6.5 and 25%(w/v) polyethylene glycol 3350	分辨率 2.60 Å R-free 0.257
5HDY The third PDZ domain from the synaptic protein PSD-95 提交 2016-01-05	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	未记录	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.9；289 K；Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against microliters of crystallization buffer (1.0 M sodium citrate) at 289 K. Diamond-shaped crystals appeared either spontaneously or with microseeding after 1-5 days and grew to 100-200 microns in length over several weeks.	分辨率 1.80 Å R-free 0.220
5HED The third PDZ domain from the synaptic protein PSD-95 in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-05	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：T-2F	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.1；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.125 M sodium citrate, pH 7.1. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.70 Å R-free 0.197
5HET The third PDZ domain from the synaptic protein PSD-95 (G330T mutant) 提交 2016-01-06	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：G330T	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Reservoir solution contained 1.05 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (7 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 2.00 Å R-free 0.213
5HEY The third PDZ domain from the synaptic protein PSD-95 (G330T mutant) in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-06	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：G330T	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (8 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.50 Å R-free 0.203
5HF1 The third PDZ domain from the synaptic protein PSD-95 (G330T mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-06	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：G330T	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.8；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 6.8. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.75 Å R-free 0.218
5HF4 The third PDZ domain from the synaptic protein PSD-95 (H372A mutant) 提交 2016-01-06	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：H372A	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Reservoir solution contained 0.95 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.75 Å R-free 0.221
5HFB The third PDZ domain from the synaptic protein PSD-95 (H372A mutant) in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-06	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：H372A	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (8 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.62 Å R-free 0.203
5HFC The third PDZ domain from the synaptic protein PSD-95 (H372A mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-06	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：H372A	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.05 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (7 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.85 Å R-free 0.207
5HFD The third PDZ domain from the synaptic protein PSD-95 (G330T, H372A double mutant) 提交 2016-01-06	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：G330T H372A	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Reservoir solution contained 1 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (13 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.60 Å R-free 0.197
5HFE The third PDZ domain from the synaptic protein PSD-95 (G330T, H372A double mutant) in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-06	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：G330T H372A	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.25 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.80 Å R-free 0.211
5HFF The third PDZ domain from the synaptic protein PSD-95 (G330T, H372A double mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-06	突变/修饰不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 302–402（101 aa）片段：PDZ-3 domain (UNP residues 302-402)	突变：G330T, H372A	GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.75；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 6.75. Equal amounts (1.5 microliters) of protein (7 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.75 Å R-free 0.221
5MZ7 Crystal Structure of the third PDZ domain from the synaptic protein PSD-95 with incorporated Azidohomoalanine 提交 2017-01-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 303–402（100 aa）	非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1.1 M sodium citrate, 0.1 M HEPES	分辨率 1.53 Å R-free 0.221
5MZ7 Crystal Structure of the third PDZ domain from the synaptic protein PSD-95 with incorporated Azidohomoalanine 提交 2017-01-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 303–402（100 aa）	非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1.1 M sodium citrate, 0.1 M HEPES	分辨率 1.53 Å R-free 0.221
5MZ7 Crystal Structure of the third PDZ domain from the synaptic protein PSD-95 with incorporated Azidohomoalanine 提交 2017-01-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 C 303–402（100 aa）	非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1.1 M sodium citrate, 0.1 M HEPES	分辨率 1.53 Å R-free 0.221
5MZ7 Crystal Structure of the third PDZ domain from the synaptic protein PSD-95 with incorporated Azidohomoalanine 提交 2017-01-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 D 303–402（100 aa）	非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1.1 M sodium citrate, 0.1 M HEPES	分辨率 1.53 Å R-free 0.221
5W72 Impact of IR active probes on PDZ3 and its ligand binding studied by NMR and X-ray crystallography 提交 2017-06-19	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 301–402（102 aa）	非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	SOLUTION NMR NMR测量条件 pH 6.8；298 K；离子强度（mmCIF原始值）0；压力 1 NMR样品组成 0.5 mM [U-98% 13C; U-98% 15N] PDZ3, 50 mM sodium phosphate, 90% H2O/10% D2O \| 90% H2O/10% D2O	分辨率未提供
5YPO Crystal structure of PSD-95 GK domain in complex with phospho-SAPAP peptide 提交 2017-11-02	构建体不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 531–713（183 aa）	未记录	GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 5.5；289 K；Condition: 0.1 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 17% w/v Polyethylene glycol 10000	分辨率 2.29 Å R-free 0.226
5YPO Crystal structure of PSD-95 GK domain in complex with phospho-SAPAP peptide 提交 2017-11-02	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 531–713（183 aa）	未记录	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 5.5；289 K；Condition: 0.1 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 17% w/v Polyethylene glycol 10000	分辨率 2.29 Å R-free 0.226
5YPR Crystal Structure of PSD-95 SH3-GK domain in complex with a synthesized inhibitor 提交 2017-11-02	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 426–721（296 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 8；277 K；0.1M tris pH 8.0, 35% tert-Butanol	分辨率 2.35 Å R-free 0.264
7CQF Crystal structure of PSD-95 PDZ3 fused with ADAM22 C-terminal peptide 提交 2020-08-10	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 信息不足单体；蛋白 × 1 PDB 声明：monomeric	链 A 309–422（114 aa）	未记录	CL CHLORIDE ION × 1 CHT CHOLINE ION × 1 EDO 1,2-ETHANEDIOL × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.2 M choline chloride, 0.1 M Tris-HCl (pH 7.5), 14 % (w/v) PEG 2000 MME	分辨率 1.80 Å R-free 0.205
7F7G a linear Peptide Inhibitors in complex with GK domain 提交 2021-06-29	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 531–713（183 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；289 K；(0.02 M Citric acid, 0.08 M BIS-TRIS propane / pH 8.8), 16% w/v Polyethylene glycol 3,350	分辨率 2.45 Å R-free 0.256
7F7G a linear Peptide Inhibitors in complex with GK domain 提交 2021-06-29	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 531–713（183 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；289 K；(0.02 M Citric acid, 0.08 M BIS-TRIS propane / pH 8.8), 16% w/v Polyethylene glycol 3,350	分辨率 2.45 Å R-free 0.256
7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 531–713（183 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)	分辨率 2.60 Å R-free 0.262
7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 531–713（183 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)	分辨率 2.60 Å R-free 0.262
7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 C 531–713（183 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)	分辨率 2.60 Å R-free 0.262
7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 D 531–713（183 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)	分辨率 2.60 Å R-free 0.262
7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 5 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 E 531–713（183 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)	分辨率 2.60 Å R-free 0.262
7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 6 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 F 531–713（183 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)	分辨率 2.60 Å R-free 0.262
9IUI Crystal structure of PSD-95 GK domain in complex with GK_FingR 提交 2024-07-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 A 531–713（183 aa）片段：GK domain	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 5；289 K；0.1M Sodium Citrate pH 5.0, 20% PEG 20000	分辨率 1.93 Å R-free 0.278
9IUI Crystal structure of PSD-95 GK domain in complex with GK_FingR 提交 2024-07-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 C 531–713（183 aa）片段：GK domain	未记录	GOL GLYCEROL × 2 PG4 TETRAETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 5；289 K；0.1M Sodium Citrate pH 5.0, 20% PEG 20000	分辨率 1.93 Å R-free 0.278
9UDJ Crystal structure of PSD95 in complex with 4-dimethylaminophenol 提交 2025-04-07	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 533–713（181 aa）片段：Guanylate kinase-like	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；289.15 K；0.1 M Sodium citrate tribasic dihydrate (pH 5.5), 18% w/v Polyethylene glycol 3,350.	分辨率 2.44 Å R-free 0.274
9UDJ Crystal structure of PSD95 in complex with 4-dimethylaminophenol 提交 2025-04-07	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 533–713（181 aa）片段：Guanylate kinase-like	未记录	A1EOW 4-(dimethylamino)phenol × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；289.15 K；0.1 M Sodium citrate tribasic dihydrate (pH 5.5), 18% w/v Polyethylene glycol 3,350.	分辨率 2.44 Å R-free 0.274

共 40 个其他 PDB 条目、58 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	DLG4_RAT
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 6–106； UniProt 302–402

Cysteine-rich PDZ-binding protein

物种未注明

UniProt Q792Q4

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 B； UniProt 93–101	片段：PDZ3-binding domain (UNP residues 93-101)	Disks large homolog 4 × 1 （P31016） GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.65 Å R-free 0.195

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 5HEB	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
5HED The third PDZ domain from the synaptic protein PSD-95 in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-05	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 93–101（9 aa）片段：PDZ3-binding domain (UNP residues 93-101)	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.1；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.125 M sodium citrate, pH 7.1. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.70 Å R-free 0.197
5HEY The third PDZ domain from the synaptic protein PSD-95 (G330T mutant) in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-06	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 93–101（9 aa）片段：PDZ3-binding domain (UNP residues 93-101)	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (8 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.50 Å R-free 0.203
5HF1 The third PDZ domain from the synaptic protein PSD-95 (G330T mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-06	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 93–101（9 aa）片段：PDZ3-binding domain (UNP residues 93-101)	突变：T99F	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.8；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 6.8. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.75 Å R-free 0.218
5HFB The third PDZ domain from the synaptic protein PSD-95 (H372A mutant) in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-06	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 93–101（9 aa）片段：PDZ3-binding domain (UNP residues 93-101)	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (8 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.62 Å R-free 0.203
5HFC The third PDZ domain from the synaptic protein PSD-95 (H372A mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-06	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 93–101（9 aa）片段：PDZ3-binding domain (UNP residues 93-101)	突变：T99F	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.05 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (7 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.85 Å R-free 0.207
5HFE The third PDZ domain from the synaptic protein PSD-95 (G330T, H372A double mutant) in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-06	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 93–101（9 aa）片段：PDZ3-binding domain (UNP residues 93-101)	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.25 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.80 Å R-free 0.211
5HFF The third PDZ domain from the synaptic protein PSD-95 (G330T, H372A double mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-06	突变/修饰不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric	链 B 93–101（9 aa）片段：PDZ3-binding domain (UNP residues 93-101)	突变：T99F	GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.75；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 6.75. Equal amounts (1.5 microliters) of protein (7 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.	分辨率 1.75 Å R-free 0.221

共 7 个其他 PDB 条目、7 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	CRIPT_RAT
Isoform	—
PDB实体	2
链与序列区间	作者链 B； PDB构建体 1–9； UniProt 93–101

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

2. 结构基本信息 2. Structure Basics

条目编号 entry_id	5heb
沉积日期 deposition_date	2016-01-05
结构标题 title	The third PDZ domain from the synaptic protein PSD-95 in complex with a C-terminal peptide derived from CRIPT
关键词 keywords	PDZ, GLGF, DHR, adhesion, synapse, synaptic density, peptide-binding domain, PEPTIDE BINDING PROTEIN; PEPTIDE BINDING PROTEIN
实验方法 method	X-RAY DIFFRACTION

▼

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	15.98 Å
回转半径 Rg (电子) rg_electron	14.64 Å
零角强度 I(0) i0	3907940.00
分子量 molecular_weight	13488.0 kDa
排除体积 excluded_volume	16713 Å³
包络体积 envelope_volume	19440 Å³
水化壳体积 shell_volume	11718 Å³
包络直径 envelope_diameter	57.7 Å
壳层 Rg shell_rg	20.08 Å
包络 Rg envelope_rg	15.18 Å
形状 Rg shape_rg	14.62 Å
总 Rg total_rg	15.80 Å
总原子数 total_atoms	1880
残基数 n_residues	126
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	58.8 Å
Rg (实空间) rg_real	15.97 Å
Rg 误差 (实空间) rg_real_error	0.42 Å
I(0) (实空间) i0_real	3.9080e+06
I(0) 误差 (实空间) i0_real_error	5.2120e+04
Rg (倒空间) rg_reciprocal	15.97 Å
I(0) (倒空间) i0_reciprocal	3908000.0000
解质量估计 total_estimate	0.8267
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	19.4 Å
偏度 Skewness skewness	0.386
峰度 Kurtosis kurtosis	0.068
角度范围 angular_range	— – 0.5000 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	623300.0000
实空间数据点数 n_real_points	80
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.617; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.912; Smooth: 0.980

▶

5. 晶体学与实验 5. Crystallography & Experiment

▶

6. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

描述 description	Disks large homolog 4
分子量 formula_weight	12738.1 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	2300

GSPEFLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEANSRVDSSGRIVTD

来源 Source

来源生物 organism	Rattus norvegicus
通用名 common_name	Rat
NCBI 分类编号 ncbi_taxonomy_id	10116
宿主生物 host_organism	Escherichia coli

实体 2 Entity 2 polymer

描述 description	Cysteine-rich PDZ-binding protein
分子量 formula_weight	1070.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	B

TKNYKQTSV

实体 3 Entity 3 non-polymer

描述 description	GLYCEROL
分子量 formula_weight	92.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 4 Entity 4 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	122

▼

7. 折叠分类 (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

结构域编号 domain_id	5hebA00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	42 — Pdz3 Domain
同源超家族 H-superfamily homologous superfamily	10 — PDZ domain

▶

8. 引用文献 (1)

▶

晶胞边长 a length_a	89.771 Å
晶胞边长 b length_b	89.771 Å
晶胞边长 c length_c	89.771 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	24

空间群 space_group	P 41 3 2
空间群编号 int_tables_number	213
晶系 crystal_system	—

分辨率 resolution	1.650 Å
最低分辨率 resolution_low	40.147 Å
R 因子 r_factor	0.1694
R_work r_work	0.1666
R_free r_free	0.1945

fit	/app/data/saxs_computation/he/5heb/5heb.fit
pddf	/app/data/pr_computation/he/5heb/5heb.pr.out
profile	/app/data/saxs_computation/he/5heb/5heb.dat

The third PDZ domain from the synaptic protein PSD-95 in complex with a C-terminal peptide derived from CRIPT

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Disks large homolog 4

当前结构中的状态

数据库中的同蛋白其他状态

Cysteine-rich PDZ-binding protein

当前结构中的状态

数据库中的同蛋白其他状态

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

2. 结构基本信息 2. Structure Basics

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

5. 晶体学与实验 5. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

6. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 water

7. 折叠分类 (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

8. 引用文献 (1)

9. 文件与曲线 (10)

Files (7)

Curves (3)