5u0s

Cryo-EM structure of the Mediator-RNAPII complex

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Mediator complex subunit 6

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED6_SCHPO
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain F； PDBConstruct 1–216； UniProt 1–216

Mediator complex subunit 8

OrganismNot specified

UniProt O94646

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED8_SCHPO
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain H； PDBConstruct 1–200； UniProt 1–200

Mediator complex subunit 17

OrganismNot specified

UniProt P87306

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED17_SCHPO
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain Q； PDBConstruct 1–545； UniProt 1–545

Mediator complex subunit 18

OrganismNot specified

UniProt O14198

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED18_SCHPO
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain R； PDBConstruct 1–207； UniProt 1–207

Mediator complex subunit 20

OrganismNot specified

UniProt Q10317

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED20_SCHPO
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain T； PDBConstruct 1–193； UniProt 1–193

Mediator complex subunit 11

OrganismNot specified

UniProt Q9P6Q0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED11_SCHPO
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain K； PDBConstruct 1–111； UniProt 1–111

Mediator complex subunit 22

OrganismNot specified

UniProt O14010

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED22_SCHPO
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain V； PDBConstruct 1–136； UniProt 1–136

Mediator complex subunit 14

OrganismNot specified

UniProt Q9P7Y4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED14_SCHPO
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain N； PDBConstruct 1–879； UniProt 1–879

Mediator complex subunit 7

OrganismNot specified

UniProt O60104

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED7_SCHPO
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain G； PDBConstruct 1–376； UniProt 1–376

Mediator complex subunit 21

OrganismNot specified

UniProt O94376

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED21_SCHPO
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain U； PDBConstruct 1–138； UniProt 1–138

Mediator complex subunit 31

OrganismNot specified

UniProt Q9USH1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED31_SCHPO
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain 3； PDBConstruct 1–139； UniProt 1–139

Mediator complex subunit 27

OrganismNot specified

UniProt Q10477

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MED27_SCHPO
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain 2； PDBConstruct 1–273； UniProt 1–273

Mediator complex subunit 9

OrganismNot specified

UniProt O13964

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	YE44_SCHPO
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain I； PDBConstruct 1–121； UniProt 1–121

RNA polymerase II subunit Rpb1

OrganismNot specified

UniProt P36594

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB1_SCHPO
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain a； PDBConstruct 1–1752； UniProt 1–1752

RNA polymerase II subunit Rpb2

OrganismNot specified

UniProt Q02061

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB2_SCHPO
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain b； PDBConstruct 1–1210； UniProt 1–1210

RNA polymerase II subunit Rpb3

OrganismNot specified

UniProt P37382

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB3_SCHPO
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain c； PDBConstruct 1–297； UniProt 1–297

RNA polymerase II subunit Rpb4

OrganismNot specified

UniProt O74825

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB4_SCHPO
Isoform	—
PDB entities	20
Chains and sequence ranges	Author chain d； PDBConstruct 1–135； UniProt 1–135

RNA polymerase II subunit Rpb5

OrganismNot specified

UniProt Q09191

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB1_SCHPO
Isoform	—
PDB entities	21
Chains and sequence ranges	Author chain e； PDBConstruct 1–210； UniProt 1–210

RNA polymerase II subunit Rpb6

OrganismNot specified

UniProt P36595

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB2_SCHPO
Isoform	—
PDB entities	22
Chains and sequence ranges	Author chain f； PDBConstruct 1–142； UniProt 1–142

RNA polymerase II subunit Rpb7

OrganismNot specified

UniProt O14459

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB7_SCHPO
Isoform	—
PDB entities	23
Chains and sequence ranges	Author chain g； PDBConstruct 1–172； UniProt 1–172

RNA polymerase II subunit Rpb8

OrganismNot specified

UniProt Q92399

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB3_SCHPO
Isoform	—
PDB entities	24
Chains and sequence ranges	Author chain h； PDBConstruct 1–125； UniProt 1–125

RNA polymerase II subunit Rpb9

OrganismNot specified

UniProt O74635

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB9_SCHPO
Isoform	—
PDB entities	25
Chains and sequence ranges	Author chain i； PDBConstruct 1–113； UniProt 1–113

RNA polymerase II subunit Rpb10

OrganismNot specified

UniProt O13877

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb11 × 1 （P87123） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB5_SCHPO
Isoform	—
PDB entities	26
Chains and sequence ranges	Author chain j； PDBConstruct 1–71； UniProt 1–71

RNA polymerase II subunit Rpb11

OrganismNot specified

UniProt P87123

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb12 × 1 （P48011）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB11_SCHPO
Isoform	—
PDB entities	27
Chains and sequence ranges	Author chain k； PDBConstruct 1–123； UniProt 1–123

RNA polymerase II subunit Rpb12

OrganismNot specified

UniProt P48011

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 28	Mediator complex subunit 6 × 1 （Q9US45） Mediator complex subunit 8 × 1 （O94646） Mediator complex subunit 17 × 1 （P87306） Mediator complex subunit 18 × 1 （O14198） Mediator complex subunit 20 × 1 （Q10317） Mediator complex subunit 11 × 1 （Q9P6Q0） Mediator complex subunit 22 × 1 （O14010） Mediator complex subunit 14 × 1 （Q9P7Y4） Mediator complex subunit 4 × 1 Mediator complex subunit 7 × 1 （O60104） Mediator complex subunit 21 × 1 （O94376） Mediator complex subunit 31 × 1 （Q9USH1） Mediator complex subunit 27 × 1 （Q10477） Mediator complex subunit 9 × 1 （O13964） Mediator complex subunit 19 × 1 Mediator complex subunit 10 × 1 RNA polymerase II subunit Rpb1 × 1 （P36594） RNA polymerase II subunit Rpb2 × 1 （Q02061） RNA polymerase II subunit Rpb3 × 1 （P37382） RNA polymerase II subunit Rpb4 × 1 （O74825） RNA polymerase II subunit Rpb5 × 1 （Q09191） RNA polymerase II subunit Rpb6 × 1 （P36595） RNA polymerase II subunit Rpb7 × 1 （O14459） RNA polymerase II subunit Rpb8 × 1 （Q92399） RNA polymerase II subunit Rpb9 × 1 （O74635） RNA polymerase II subunit Rpb10 × 1 （O13877） RNA polymerase II subunit Rpb11 × 1 （P87123）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB4_SCHPO
Isoform	—
PDB entities	28
Chains and sequence ranges	Author chain l； PDBConstruct 1–63； UniProt 1–63

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	5u0s
Deposition date deposition_date	2016-11-26
Structure title title	Cryo-EM structure of the Mediator-RNAPII complex
Keywords keywords	transcriptional, transcription, Mediator, Srb, RNA polymerase II, RNA, Pol2, activation, complex, TRANSCRIPTION-TRANSFERASE complex; TRANSCRIPTION/TRANSFERASE
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5u0s__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5u0s__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

5u0s__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	72.12 Å
Rg (electron density)	70.98 Å
Total Rg	70.52 Å
Atom count	51548
Residues	6861
Excluded volume	909690 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`5u0s__assembly_1__model_1`	28-meric （28）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (28)

Entity 1 Entity 1 polymer

Description description	Mediator complex subunit 6
Molecular weight formula_weight	24928.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F
Atom count atom_count	51548

MASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNALDLGDLNSQLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENKNQSIENADYSFSPEDFSVVRAFMQSLHSSKEAPDVK

Entity 2 Entity 2 polymer

Description description	Mediator complex subunit 8
Molecular weight formula_weight	23360.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MEDISTEKTVESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSLSNNLAAHSHTLQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISSQPKKKEANDAYQKDQLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATEEQNANQMLTDILSFMKSGKR

Entity 3 Entity 3 polymer

Description description	Mediator complex subunit 17
Molecular weight formula_weight	62551.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MAEEANKDADISSLSLSLDPEIIGGQNNFLENNLQQIFQKIIQERGPFRDLKEEDLQKELQKESIKDESSAKSSETENVLEFATLDSKRNVNDTEVESMDSQAYKKELIEQIMIAQTECSLALDMTSLLLSKFKENSIETISPFLKSTVPPSSLQFSRSQPPESKESDATLAKCWKEKSLTSSCKFLFEAKERLTSVVETEHEYYTELVKVKEASWPLFNSQGSNHLSVQYSCLGGISLGLGLIRMKPESKSFEVQSSLLYSQAALKISILNKDRDEIGSSTWSWPSQNCNSVLLKDIYKLQEILFEMDIWNSLLQEAQSCGNQGVNFTGDEILVPISDDHVVRITLETSSKNTESGFTEDKKSNEDTSTNFVTIKQEKELLKCLCDTLNAIAHILFLKHCRKSDRRSQQPELYMAIDANAPLILRPLIFYYNLNQESLEFQRWLKQRDISFKFMPNYPWEKAKDFLELENSLSINRLSISWRIMVSNFEPAIFIQHTPTLHGTDKSVWRCKDQYSSNQFSSLKNVCQYIEHHINSLSRRSKKTE

Entity 4 Entity 4 polymer

Description description	Mediator complex subunit 18
Molecular weight formula_weight	24056.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MQELYLLGVVPSRRFEAVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQPDSWLRLCSNIESHDETDTEWSKNTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEFSHEYIIQGLEYFFFDTTVRIYQTLIPSQQRSIKPPFHPMNEEQPWILHVYTHVADASNQVAMAKAEANLTKVKTLLSAFCDLKNVRL

Entity 5 Entity 5 polymer

Description description	Mediator complex subunit 20
Molecular weight formula_weight	22374.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T

MPVHGVIYYSSPSMATFLSPAQDNLVRTYFAQHLKKWVVQYKLYRNAVTPKTLEFLKQNINPSMLACVDEATMIDAEPELEDIIVRTKLWNFRQSFTIEGSIYEVGSFKVAIANVLQKSVWKGILFHVTYDGTESVDLARPIIQEFFLKCFLQNNKSVTPVYESFFNQPRHSLDSKLLLQLFKQRIDTVSQRT

Entity 6 Entity 6 polymer

Description description	Mediator complex subunit 11
Molecular weight formula_weight	13118.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MTNSDDDLFSEKSTSSDTQQVQNILELEAKIPDILSSAGKCIEAIQLNNSLEDFRKYSKEFLETVEFISTGLRRQALELEKAEVPVVSLQPKKRYASTPLSNLIFDQSSKLIPKYC

Entity 7 Entity 7 polymer

Description description	Mediator complex subunit 22
Molecular weight formula_weight	15396.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	V

MSSDSFQRQLVQRTNTLNSSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQIECHTVSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLIEPEASETTEAPTVSDT

Entity 8 Entity 8 polymer

Description description	Mediator complex subunit 14
Molecular weight formula_weight	105382.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MEPPAIPHITEGFYPLPEIVETFSHHVLQELVSLAEVLPSMSNVEKKKKILDWLLRSRAFTMRLLVLARWVHLSPSVHRCIDVVAFLQGQKFCFQNLVHVLQDIRYQLSFARLRNSDLVTALDILSTGTSLRLANAPTSKLYMLSESPLSTKQILQTLHALNMLIRIRLSLYEIIPTPFQHFTIANGRCTFTVPNEFSVSLTTNSQDPKSTGISFQWIVVDFQFHLPDFSSTPAKYRVFIELHLNEEIAAAFVLQKPILPLIYNILHKFCLYQRLNLLSQQTFQLSRESWLGHLRGVYDEKPPRLRLYYWPQLNVKKEGKPGKIGHYIHIFVNTQPISAFERTLSSKRSSCEYDHFLLLVEWHHDGIVEHVPLDDHMDAQHLLLLITQKHAQLILEQIRKELHPNIFSEHVGGGLKIHVFDNEIIVKVNSVTGRLVLSSSASPLSPPRHLRAAEKNIALNTQPPAQILNRLYFFCIQTQLLEVAQCAELHAVQGYYSFPYLTFSKGKWRKDGDSLWVLAYNVESNSWSVRLLNAAGQTLYTQDVHTTKGTLSIESFSRLSYLLEVQILLFNVQTACQARGMPFEYLPIPPKALIEDDFTTYVQTGCLCIMMPSSNEDMLPVVFVRAHDGQLIFDSRIKGKLPYQSETETEKNCYIDWRTGRITIRVQNFSSFEKTWIGLLKLVALSKTSAFNVDCITLKHVDFTYLDDEKFRATIHDDNTFTLHFFNRHSPFHLISQFLQDTFSDGPSAIQPLRVIMDRTRGVLVAQELGYVVLARSLRQYRIILSKNHGIQVLLNRHGCILQDLSYLSADSRYLEGTQTLTSQWEPCSWLNTVWEGDLGDDELNGQIEAAPEMHLIKMNKTADLTAILKRILAISRKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 9 Entity 9 polymer

Description description	Mediator complex subunit 4
Molecular weight formula_weight	20358.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 10 Entity 10 polymer

Description description	Mediator complex subunit 7
Molecular weight formula_weight	43573.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MEPNEGVQLFSAFPPPPPYYKLFTRENIEKVISNMEKEAKHEDDANTLQPKTEEEIESLAKLFKKPSCLTSGTYQMFGDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEYDPKSNAIVGTSFTRVHDYDKNSPIENEKILEDDQHTAMKEKDNESDKTMKDVEEKTEPSLKKEEEDIQMKEPLDSQDTGAVSASSVNEGFRADQKSKDGETSDLIKIPRRAYELRFLSRSLMLNFLELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVELLRTHNRQMTETLEKYKSLDFNMEKEGDVIQQLKSSIKKPLSGAEDEQKSRSMFSKNDEKLKKSLELMEDVIKRDLS

Entity 11 Entity 11 polymer

Description description	Mediator complex subunit 21
Molecular weight formula_weight	15821.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRDLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQLAKRIETFGRLSCVLFQP

Entity 12 Entity 12 polymer

Description description	Mediator complex subunit 31
Molecular weight formula_weight	16914.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	3

METKWLLSKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREPEYVKFIIYPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIYYEWLGKGLQQYGSADDATLSQPQQEEDEKKVDVKKENE

Entity 13 Entity 13 polymer

Description description	Mediator complex subunit 27
Molecular weight formula_weight	31132.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	2

MSLEEQRTRDELKHKLLDLNQLHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEKVNSFYTHLKSSQNVFDYAEKSLQADSSNLLPTYLYNSEDLSNDTENNETKSINGKSALDLKEPHHSELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFTLKLANELEFIFFHDTRGKFSVYCSSSKDDAITFSINRNNNFLGNLWSLMPKILDYQHLYSKPCDFCKSLISPVYLELPSVRRNANSTVKPTSKDILALHAECVPAQSDL

Entity 14 Entity 14 polymer

Description description	Mediator complex subunit 9
Molecular weight formula_weight	13811.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MDPAHHIKDTSAELLSQQDFSILQSRLLEFLASQEARETVTASKELTLLRQGIRQLKEKVSKMEPEEMTVKEKKSIIEILKARIALKKAFLKMALSESNDTVVKKEDVRESPVDTFMENAT

Entity 15 Entity 15 polymer

Description description	Mediator complex subunit 19
Molecular weight formula_weight	11847.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 16 Entity 16 polymer

Description description	Mediator complex subunit 10
Molecular weight formula_weight	11251.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 17 Entity 17 polymer

Description description	RNA polymerase II subunit Rpb1
Molecular weight formula_weight	194372.6 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	a

MSGIQFSPSSVPLRRVEEVQFGILSPEEIRSMSVAKIEFPETMDESGQRPRVGGLLDPRLGTIDRQFKCQTCGETMADCPGHFGHIELAKPVFHIGFLSKIKKILECVCWNCGKLKIDSSNPKFNDTQRYRDPKNRLNAVWNVCKTKMVCDTGLSAGSDNFDLSNPSANMGHGGCGAAQPTIRKDGLRLWGSWKRGKDESDLPEKRLLSPLEVHTIFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDIIKANANVRRCEQEGAPAHIVSEYEQLLQFHVATYMDNEIAGQPQALQKSGRPLKSIRARLKGKEGRLRGNLMGKRVDFSARTVITGDPNLSLDELGVPRSIAKTLTYPETVTPYNIYQLQELVRNGPDEHPGAKYIIRDTGERIDLRYHKRAGDIPLRYGWRVERHIRDGDVVIFNRQPSLHKMSMMGHRIRVMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPVMGIVQDTLAGVRKFSLRDNFLTRNAVMNIMLWVPDWDGILPPPVILKPKVLWTGKQILSLIIPKGINLIRDDDKQSLSNPTDSGMLIENGEIIYGVVDKKTVGASQGGLVHTIWKEKGPEICKGFFNGIQRVVNYWLLHNGFSIGIGDTIADADTMKEVTRTVKEARRQVAECIQDAQHNRLKPEPGMTLRESFEAKVSRILNQARDNAGRSAEHSLKDSNNVKQMVAAGSKGSFINISQMSACVGQQIVEGKRIPFGFKYRTLPHFPKDDDSPESRGFIENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKAMEDVMVRYDGTVRNAMGDIIQFAYGEDGLDATLVEYQVFDSLRLSTKQFEKKYRIDLMEDRSLSLYMENSIENDSSVQDLLDEEYTQLVADRELLCKFIFPKGDARWPLPVNVQRIIQNALQIFHLEAKKPTDLLPSDIINGLNELIAKLTIFRGSDRITRDVQNNATLLFQILLRSKFAVKRVIMEYRLNKVAFEWIMGEVEARFQQAVVSPGEMVGTLAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPSLTIYLMPWIAANMDLAKNVQTQIEHTTLSTVTSATEIHYDPDPQDTVIEEDKDFVEAFFAIPDEEVEENLYKQSPWLLRLELDRAKMLDKKLSMSDVAGKIAESFERDLFTIWSEDNADKLIIRCRIIRDDDRKAEDDDNMIEEDVFLKTIEGHMLESISLRGVPNITRVYMMEHKIVRQIEDGTFERADEWVLETDGINLTEAMTVEGVDATRTYSNSFVEILQILGIEATRSALLKELRNVIEFDGSYVNYRHLALLCDVMTSRGHLMAITRHGINRAETGALMRCSFEETVEILMDAAASGEKDDCKGISENIMLGQLAPMGTGAFDIYLDQDMLMNYSLGTAVPTLAGSGMGTSQLPEGAGTPYERSPMVDSGFVGSPDAAAFSPLVQGGSEGREGFGDYGLLGAASPYKGVQSPGYTSPFSSAMSPGYGLTSPSYSPSSPGYSTSPAYMPSSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS

Entity 18 Entity 18 polymer

Description description	RNA polymerase II subunit Rpb2
Molecular weight formula_weight	138027.2 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	b

MSYEDYQYNETLTQEDCWTVISSFFEETSLARQQLFSFDEFVQNTMQEIVDDDSTLTLDQYAQHTGAQGDVTRRYEINFGQIYLSRPTMTEADGSTTTMFPQEARLRNLTYSSPLYVDMRKKVMVAADSNVPIGEEEWLVEEEDEEPSKVFIGKIPIMLRSTFCILNGVSDSELYDLNECPYDQGGYFIINGSEKVIIAQERSAANIVQVFKKAAPSPIAYVAEIRSALERGSRLISSMQIKLMARNTENSGQTIRATLPYIRSDIPIVIVFRALGVVPDRDILEHICYDPNDFQMLEMMKPCIEEAFVIQDKDIALDYIGKRGSTTGVTREKRLRYAHDILQKELLPHITTMEGFETRKAFFLGYMIHRMLLCALERREPDDRDHFGKKRLDLAGPLLASLFRMLFRKMTRDVYKYMQKCVETNREFNLTLAVKSNIITNGLRYSLATGNWGDQKRSMVNRVGVSQVLNRYTFASTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGMVCPAETPEGQACGLVKNLSLMSYVSVGSPSAPIIEFLEEWGLETLEDYNPSASPNATKVFVNGVWLGVHRDPAHLTETLRSLRRRLDISAEVSIVRDIREKELRLFTDAGRICRPLFIVDNNPNSERRGELCIRKEHIQQLIEDKDRYDIDPEQRFGWTALVSSGLIEYLDAEEEETVMIAMSPEDLEASRQMQAGYEVKEELDPAQRVKPAPNPHVHAWTHCEIHPAMILGILASIIPFPDHNQSPRNTYQSAMGKQAMGVYLTNYQVRMDTMANILYYPQKPLATTRSMEYLKFRELPAGQNAIVAILCYSGYNQEDSIIMNQASIDRGLFRSIFYRTYTDQEKKIGMTVMEEFERPVRSTTLRMKHGTYDKLEDDGLIAPGTRVSGEDIIIGKTAPIPLDHEELGQRTQLHAKRDVSTPLRSTESGIVDQVMVTTNQEGLKFVKVRMRSTRIPQIGDKFASRHGQKGTIGMTYRHEDMPFSAQGIVPDIIINPHAIPSRMTVAHLVECQLSKVSALSGFEGDATPFTDVTVEAVSKLLRSHGFQSRGFEVMYHGHTGRKLVAQVFLGPTYYQRLKHLVDDKIHARARGPVQILTRQPVEGRSRDGGLRFGEMERDCQISHGCSSVLRERLFDCSDAYRVIVCDICGLIAIASYKKDSYECRSCQNRTRFSQVYLPYAAKLLFQELMSMNIAPRLFTKNHK

Entity 19 Entity 19 polymer

Description description	RNA polymerase II subunit Rpb3
Molecular weight formula_weight	33748.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	c

MDSETHITIRNISKNSVDFVLTNTSLAVANSLRRVVLAEIPTVAIDLVEINVNTSVMPDEFLAHRLGMIPLDSSNIDEPPPVGLEYTRNCDCDQYCPKCSVELFLNAKCTGEGTMEIYARDLVVSSNSSLGHPILADPKSRGPLICKLRKEQEISLRCIAKKGIAKEHAKWSPTSAVAFEYDPWNKLQHTDYWFENDADAEWPKSKNADWEEPPREGEPFNFQEEPRRFYMDVESVGSIPPNEIMVQGLRILQEKLAVLVRDLDEEQPTQLSANELNMEENAEMNWSPYQNGEENTW

Entity 20 Entity 20 polymer

Description description	RNA polymerase II subunit Rpb4
Molecular weight formula_weight	15379.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	d

MPRAIFEEDAAQLKLGPEFENEDMLTVSEAKILIETVLAQRARETNGEIPMTDVMKKTVAYFNVFARFKTAEATYACERILGNRFHKFERAQLGTLCCEDAEEARTLIPSLANKIDDQNLQGILDELSTLRKFQD

Entity 21 Entity 21 polymer

Description description	RNA polymerase II subunit Rpb5
Molecular weight formula_weight	23954.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	e

MSAEEKNIVRVFRAWKTAHQLVHDRGYGVSQAELDLTLDQFKAMHCGMGRNLDRTTLSFYAKPSNDSNKGTIYIEFAKEPSVGIKEMRTFVHTLGDHNHKTGILIYANSMTPSAAKIIATVTGQFTIETFQESDLIVNITHHELVPKHILLSPDEKKELLDRYKLRETQLPRIQLADPVARYLGLKRGEVVKIVRRSETSGRYNSYRICA

Entity 22 Entity 22 polymer

Description description	RNA polymerase II subunit Rpb6
Molecular weight formula_weight	15742.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	f

MSDYEEDEAFGMDGAVMEEEVDELEMIDENGQSQQGVSHPGEPSTTVITEDVASSKTAQSGKAVAKEDRTTTPYMTKYERARILGTRALQISMNAPVLVDLEGETDPLQIAMKELAQKKIPLLVRRYLPDGSYEDWSVAELI

Entity 23 Entity 23 polymer

Description description	RNA polymerase II subunit Rpb7
Molecular weight formula_weight	19122.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	g

MPFFLKELSLTISLHPSYFGPRMQDYLKAKLLADVEGTCSGQYGYIICVLDSNTIDIDKGRVVPGQGFAEFEVKYRAVLWRPFRGEVVDAIVTTVNKMGFFANIGPLNVFVSSHLVPPDMKFDPTANPPNYSGEDQVIEKGSNVRLKIVGTRTDATEIFAIATMKEDYLGVL

Entity 24 Entity 24 polymer

Description description	RNA polymerase II subunit Rpb8
Molecular weight formula_weight	14317.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	h

MSESVLLDEIFTVTSVDKQKYQRVSRITAVSGQNDMNLTLDINSQIYPLEKDATFSLQITSNLNSPDLKEAADYIMYGKVYRVEEAKDEKVSVYVSFGGLLMAIEGSHRKLYRLSLDHVYLLLRR

Entity 25 Entity 25 polymer

Description description	RNA polymerase II subunit Rpb9
Molecular weight formula_weight	13192.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	i

MSNFQYCIECNNMLYPREDKVDRVLRLACRNCDYSEIAATSKVYRHELQSSNVENTTVSHDASTDPTLPRSDKECPRCHQHEAVFYQTHSRRGDTMMTLIYVCVHCGFAFEEQ

Entity 26 Entity 26 polymer

Description description	RNA polymerase II subunit Rpb10
Molecular weight formula_weight	8286.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	j

MIIPIRCFSCGKVIGDKWDTYLTLLQEDNTEGEALDKLGLQRYCCRRMILTHVDLIEKLLCYNPLSKQKNL

Entity 27 Entity 27 polymer

Description description	RNA polymerase II subunit Rpb11
Molecular weight formula_weight	14143.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	k

MNQPERYELIELMGLPKVTYELDSKSPNAAVVTLEKEDHTLANMLANQLLSDERVLFAGYKVPHPLNHNFILRVQTVEDCSPKQVIVDAAKSLITHLEEIKVNFMREWELKMISVEGVEMEFS

Entity 28 Entity 28 polymer

Description description	RNA polymerase II subunit Rpb12
Molecular weight formula_weight	7216.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	l

MNHPTSTGGTAFNPPRPATMIYLCADCGARNTIQAKEVIRCRECGHRVMYKMRTKRMVQFEAR

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	7.80 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	3862

Cryo-EM structure of the Mediator-RNAPII complex

1. Protein Identity and Related Structures Protein Identity & Related Structures

Mediator complex subunit 6

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mediator complex subunit 8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mediator complex subunit 17

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mediator complex subunit 18

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mediator complex subunit 20

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mediator complex subunit 11

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mediator complex subunit 22

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mediator complex subunit 14

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mediator complex subunit 7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Mediator complex subunit 21

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer