8qsz

Structure of s. pombe RNA polymerase II in complex with DSIF and Rat1/Rai1

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase II subunit rpb1

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB1_SCHPO
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–1752； UniProt 1–1752

DNA-directed RNA polymerase II subunit RPB2

OrganismNot specified

UniProt Q02061

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB2_SCHPO
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–1210； UniProt 1–1210

DNA-directed RNA polymerase II subunit RPB3

OrganismNot specified

UniProt P37382

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB3_SCHPO
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–297； UniProt 1–297

DNA-directed RNA polymerases I, II, and III subunit RPABC1

OrganismNot specified

UniProt Q09191

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB1_SCHPO
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain E； PDBConstruct 1–210； UniProt 1–210

DNA-directed RNA polymerases I, II, and III subunit RPABC2

OrganismNot specified

UniProt P36595

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB2_SCHPO
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain F； PDBConstruct 1–142； UniProt 1–142

DNA-directed RNA polymerase II subunit rpb7

OrganismNot specified

UniProt O14459

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB7_SCHPO
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain G； PDBConstruct 1–172； UniProt 1–172

DNA-directed RNA polymerases I, II, and III subunit RPABC3

OrganismNot specified

UniProt Q92399

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB3_SCHPO
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain H； PDBConstruct 1–125； UniProt 1–125

DNA-directed RNA polymerase II subunit RPB9,RBP9

OrganismNot specified

UniProt O74635

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB9_SCHPO
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain I； PDBConstruct 1–113； UniProt 1–113

DNA-directed RNA polymerases I, II, and III subunit RPABC5

OrganismNot specified

UniProt O13877

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB5_SCHPO
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain J； PDBConstruct 1–71； UniProt 1–71

DNA-directed RNA polymerase II subunit RPB11

OrganismNot specified

UniProt P87123

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB11_SCHPO
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain K； PDBConstruct 1–123； UniProt 1–123

DNA-directed RNA polymerases I, II, and III subunit RPABC4

OrganismNot specified

UniProt P48011

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB4_SCHPO
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain L； PDBConstruct 1–63； UniProt 1–63

Transcription elongation factor spt5

Schizosaccharomyces pombe

UniProt O13936

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ;5'-3' exoribonuclease 2 ; × 1 （P40848） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SPT5_SCHPO
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain Y； PDBConstruct 133–1122； UniProt 1–990

;5'-3' exoribonuclease 2 ;

Schizosaccharomyces pombe

UniProt P40848

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit rpb1 × 1 （P36594） DNA-directed RNA polymerase II subunit RPB2 × 1 （Q02061） DNA-directed RNA polymerase II subunit RPB3 × 1 （P37382） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （Q09191） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P36595） DNA-directed RNA polymerase II subunit rpb7 × 1 （O14459） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （Q92399） DNA-directed RNA polymerase II subunit RPB9,RBP9 × 1 （O74635） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （O13877） DNA-directed RNA polymerase II subunit RPB11 × 1 （P87123） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P48011） Transcription elongation factor spt5 × 1 （O13936） DNA none template (34-MER) × 1 ;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ; × 1 DNA template (40-MER) × 1 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	XRN2_SCHPO
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain X； PDBConstruct 1–893； UniProt 1–893

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8qsz
Deposition date deposition_date	2023-10-12
Structure title title	Structure of s. pombe RNA polymerase II in complex with DSIF and Rat1/Rai1
Keywords keywords	RNA polymerase II, Rai1, Rat1, Spt5, DSIF, Termination, exoribonuclease, RNA BINDING PROTEIN; RNA BINDING PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8qsz__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8qsz__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8qsz__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	49.25 Å
Rg (electron density)	48.48 Å
Total Rg	48.76 Å
Atom count	62805
Residues	3857
Excluded volume	560210 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8qsz__assembly_1__model_1`	hexadecameric （16）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (17)

Entity 1 Entity 1 polymer

Description description	DNA-directed RNA polymerase II subunit rpb1
Molecular weight formula_weight	194372.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	62805

MSGIQFSPSSVPLRRVEEVQFGILSPEEIRSMSVAKIEFPETMDESGQRPRVGGLLDPRLGTIDRQFKCQTCGETMADCPGHFGHIELAKPVFHIGFLSKIKKILECVCWNCGKLKIDSSNPKFNDTQRYRDPKNRLNAVWNVCKTKMVCDTGLSAGSDNFDLSNPSANMGHGGCGAAQPTIRKDGLRLWGSWKRGKDESDLPEKRLLSPLEVHTIFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDIIKANANVRRCEQEGAPAHIVSEYEQLLQFHVATYMDNEIAGQPQALQKSGRPLKSIRARLKGKEGRLRGNLMGKRVDFSARTVITGDPNLSLDELGVPRSIAKTLTYPETVTPYNIYQLQELVRNGPDEHPGAKYIIRDTGERIDLRYHKRAGDIPLRYGWRVERHIRDGDVVIFNRQPSLHKMSMMGHRIRVMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPVMGIVQDTLAGVRKFSLRDNFLTRNAVMNIMLWVPDWDGILPPPVILKPKVLWTGKQILSLIIPKGINLIRDDDKQSLSNPTDSGMLIENGEIIYGVVDKKTVGASQGGLVHTIWKEKGPEICKGFFNGIQRVVNYWLLHNGFSIGIGDTIADADTMKEVTRTVKEARRQVAECIQDAQHNRLKPEPGMTLRESFEAKVSRILNQARDNAGRSAEHSLKDSNNVKQMVAAGSKGSFINISQMSACVGQQIVEGKRIPFGFKYRTLPHFPKDDDSPESRGFIENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKAMEDVMVRYDGTVRNAMGDIIQFAYGEDGLDATLVEYQVFDSLRLSTKQFEKKYRIDLMEDRSLSLYMENSIENDSSVQDLLDEEYTQLVADRELLCKFIFPKGDARWPLPVNVQRIIQNALQIFHLEAKKPTDLLPSDIINGLNELIAKLTIFRGSDRITRDVQNNATLLFQILLRSKFAVKRVIMEYRLNKVAFEWIMGEVEARFQQAVVSPGEMVGTLAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPSLTIYLMPWIAANMDLAKNVQTQIEHTTLSTVTSATEIHYDPDPQDTVIEEDKDFVEAFFAIPDEEVEENLYKQSPWLLRLELDRAKMLDKKLSMSDVAGKIAESFERDLFTIWSEDNADKLIIRCRIIRDDDRKAEDDDNMIEEDVFLKTIEGHMLESISLRGVPNITRVYMMEHKIVRQIEDGTFERADEWVLETDGINLTEAMTVEGVDATRTYSNSFVEILQILGIEATRSALLKELRNVIEFDGSYVNYRHLALLCDVMTSRGHLMAITRHGINRAETGALMRCSFEETVEILMDAAASGEKDDCKGISENIMLGQLAPMGTGAFDIYLDQDMLMNYSLGTAVPTLAGSGMGTSQLPEGAGTPYERSPMVDSGFVGSPDAAAFSPLVQGGSEGREGFGDYGLLGAASPYKGVQSPGYTSPFSSAMSPGYGLTSPSYSPSSPGYSTSPAYMPSSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS

Entity 2 Entity 2 polymer

Description description	DNA-directed RNA polymerase II subunit RPB2
Molecular weight formula_weight	138027.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MSYEDYQYNETLTQEDCWTVISSFFEETSLARQQLFSFDEFVQNTMQEIVDDDSTLTLDQYAQHTGAQGDVTRRYEINFGQIYLSRPTMTEADGSTTTMFPQEARLRNLTYSSPLYVDMRKKVMVAADSNVPIGEEEWLVEEEDEEPSKVFIGKIPIMLRSTFCILNGVSDSELYDLNECPYDQGGYFIINGSEKVIIAQERSAANIVQVFKKAAPSPIAYVAEIRSALERGSRLISSMQIKLMARNTENSGQTIRATLPYIRSDIPIVIVFRALGVVPDRDILEHICYDPNDFQMLEMMKPCIEEAFVIQDKDIALDYIGKRGSTTGVTREKRLRYAHDILQKELLPHITTMEGFETRKAFFLGYMIHRMLLCALERREPDDRDHFGKKRLDLAGPLLASLFRMLFRKMTRDVYKYMQKCVETNREFNLTLAVKSNIITNGLRYSLATGNWGDQKRSMVNRVGVSQVLNRYTFASTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGMVCPAETPEGQACGLVKNLSLMSYVSVGSPSAPIIEFLEEWGLETLEDYNPSASPNATKVFVNGVWLGVHRDPAHLTETLRSLRRRLDISAEVSIVRDIREKELRLFTDAGRICRPLFIVDNNPNSERRGELCIRKEHIQQLIEDKDRYDIDPEQRFGWTALVSSGLIEYLDAEEEETVMIAMSPEDLEASRQMQAGYEVKEELDPAQRVKPAPNPHVHAWTHCEIHPAMILGILASIIPFPDHNQSPRNTYQSAMGKQAMGVYLTNYQVRMDTMANILYYPQKPLATTRSMEYLKFRELPAGQNAIVAILCYSGYNQEDSIIMNQASIDRGLFRSIFYRTYTDQEKKIGMTVMEEFERPVRSTTLRMKHGTYDKLEDDGLIAPGTRVSGEDIIIGKTAPIPLDHEELGQRTQLHAKRDVSTPLRSTESGIVDQVMVTTNQEGLKFVKVRMRSTRIPQIGDKFASRHGQKGTIGMTYRHEDMPFSAQGIVPDIIINPHAIPSRMTVAHLVECQLSKVSALSGFEGDATPFTDVTVEAVSKLLRSHGFQSRGFEVMYHGHTGRKLVAQVFLGPTYYQRLKHLVDDKIHARARGPVQILTRQPVEGRSRDGGLRFGEMERDCQISHGCSSVLRERLFDCSDAYRVIVCDICGLIAIASYKKDSYECRSCQNRTRFSQVYLPYAAKLLFQELMSMNIAPRLFTKNHK

Entity 3 Entity 3 polymer

Description description	DNA-directed RNA polymerase II subunit RPB3
Molecular weight formula_weight	33748.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MDSETHITIRNISKNSVDFVLTNTSLAVANSLRRVVLAEIPTVAIDLVEINVNTSVMPDEFLAHRLGMIPLDSSNIDEPPPVGLEYTRNCDCDQYCPKCSVELFLNAKCTGEGTMEIYARDLVVSSNSSLGHPILADPKSRGPLICKLRKEQEISLRCIAKKGIAKEHAKWSPTSAVAFEYDPWNKLQHTDYWFENDADAEWPKSKNADWEEPPREGEPFNFQEEPRRFYMDVESVGSIPPNEIMVQGLRILQEKLAVLVRDLDEEQPTQLSANELNMEENAEMNWSPYQNGEENTW

Entity 4 Entity 4 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC1
Molecular weight formula_weight	23954.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MSAEEKNIVRVFRAWKTAHQLVHDRGYGVSQAELDLTLDQFKAMHCGMGRNLDRTTLSFYAKPSNDSNKGTIYIEFAKEPSVGIKEMRTFVHTLGDHNHKTGILIYANSMTPSAAKIIATVTGQFTIETFQESDLIVNITHHELVPKHILLSPDEKKELLDRYKLRETQLPRIQLADPVARYLGLKRGEVVKIVRRSETSGRYNSYRICA

Entity 5 Entity 5 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC2
Molecular weight formula_weight	15742.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MSDYEEDEAFGMDGAVMEEEVDELEMIDENGQSQQGVSHPGEPSTTVITEDVASSKTAQSGKAVAKEDRTTTPYMTKYERARILGTRALQISMNAPVLVDLEGETDPLQIAMKELAQKKIPLLVRRYLPDGSYEDWSVAELI

Entity 6 Entity 6 polymer

Description description	DNA-directed RNA polymerase II subunit rpb7
Molecular weight formula_weight	19122.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MPFFLKELSLTISLHPSYFGPRMQDYLKAKLLADVEGTCSGQYGYIICVLDSNTIDIDKGRVVPGQGFAEFEVKYRAVLWRPFRGEVVDAIVTTVNKMGFFANIGPLNVFVSSHLVPPDMKFDPTANPPNYSGEDQVIEKGSNVRLKIVGTRTDATEIFAIATMKEDYLGVL

Entity 7 Entity 7 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC3
Molecular weight formula_weight	14317.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MSESVLLDEIFTVTSVDKQKYQRVSRITAVSGQNDMNLTLDINSQIYPLEKDATFSLQITSNLNSPDLKEAADYIMYGKVYRVEEAKDEKVSVYVSFGGLLMAIEGSHRKLYRLSLDHVYLLLRR

Entity 8 Entity 8 polymer

Description description	DNA-directed RNA polymerase II subunit RPB9,RBP9
Molecular weight formula_weight	16253.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MSNFQYCIECNNMLYPREDKVDRVLRLACRNCDYSEIAATSKVYRHELQSSNVENTTVSHDASTDPTLPRSDKECPRCHQHEAVFYQTHSRRGDTMMTLIYVCVHCGFAFEEQGGGGGGDYKDHDGDYKDHDIDYKDDDDK

Entity 9 Entity 9 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC5
Molecular weight formula_weight	8286.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MIIPIRCFSCGKVIGDKWDTYLTLLQEDNTEGEALDKLGLQRYCCRRMILTHVDLIEKLLCYNPLSKQKNL

Entity 10 Entity 10 polymer

Description description	DNA-directed RNA polymerase II subunit RPB11
Molecular weight formula_weight	14143.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MNQPERYELIELMGLPKVTYELDSKSPNAAVVTLEKEDHTLANMLANQLLSDERVLFAGYKVPHPLNHNFILRVQTVEDCSPKQVIVDAAKSLITHLEEIKVNFMREWELKMISVEGVEMEFS

Entity 11 Entity 11 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC4
Molecular weight formula_weight	7216.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MNHPTSTGGTAFNPPRPATMIYLCADCGARNTIQAKEVIRCRECGHRVMYKMRTKRMVQFEAR

Entity 12 Entity 12 polymer

Description description	Transcription elongation factor spt5
Molecular weight formula_weight	123489.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y

MKHHHHHHPMSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGSGSGSENLYFQGAMDTNSPKSIDKDANSTEVDAAEQDAASVKINSTRASPNGSDLLNDDSEAAKITTNEKQSSPVDSHNESPNDTTINKGEDGNENEVDNVNNNDKKEDEDNVEENEEEADANEEEEEDEEDDEEDEEDEDESGGGRRKRARHDRRNQFLDIEAEVDEDEEELEDEEDEIGREDGFIEEEVGADYVGDDRRHRELDRQRQELQSVDAERLAEEYREKYGRSQTVVGDTSNVPQRLLLPSVNDPNIWAVRCKIGKEKDIVFTIMRKAMDLQYTSSPLEIISAFQRDSLVGYIYVEARKQSHVLDALNGVLNVYTNNMILVPIKEMPDLLKVQKQVVELLPGAYVRIRRGKYAGDLAQVDNLSENGLTARVRIVPRIDYSDGLKRKNSATRPQARLFNESEAFKSNPSKFSKRGPRLFLFNNEEFEDGFLVKDIRISSLITEGVNPTLDEVSKFNPNNEDLDLSSLALSVKGGHAEFQPGDHVEVYVGEQTGVSGVVENVRGSVITMVSSDGLRLDVPSRGLRKRFRHGDYVKVIAGKYKDDTGMVVRISKDEVTFLSDTLMTELTVFSRDLGEASSAQAVNSAYELHDLVQLDVNTVACIFSVDRDTYKVIDQNGGVRTVLASQITMRHSNRRGVATDRNGAEIRIGDKVKEVGGEGKQGTILHIYRAFVFLHNRDIAENNGVFSARSRNVATIAAKGARISADLTKMNPALSNGPALPPVANLKRTIGRDKAIGATVRIRRGPMKGLLGVIKDTTDANARVELHTGNKMVTIPKENLLYTTKTGELISYTEFIERSRGIRPGSISTADGPNVPNWAQGARTPAVANGSRTPAWNTGSRTPAWNSGSKTPAWNSGSRTPAWNSGNKTPAWNAGSRTPAWNSGNKTPAWNVGNKTPAWNSGAKTPAWNAGNKTPSWNNGTKTPAWNANQTPMVANGTNTSWGQTPAYGGFSETNWDTEDNSKPYTAPTPGAWAAPTPGGWDDEEGDSPKYVPPSP

Source Source

Source organism organism	Schizosaccharomyces pombe
Common name common_name	fission yeast
NCBI taxonomy ID ncbi_taxonomy_id	4896
Host organism host_organism	Escherichia coli

Entity 13 Entity 13 polymer

Description description	DNA none template (34-MER)
Molecular weight formula_weight	14917.5 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	N

CCGTGTCTAGCACAGGGAAATGGTTTGTGTCTGCTTATCGGTAGAGTG

Entity 14 Entity 14 polymer

Description description	;RNA (5'-R(PAPUPCPGPAPGPAPGPGPU)-3') ;
Molecular weight formula_weight	8245.8 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	P

UGAAUCUAUUUCUUUUAUCGAGAGGU

Entity 15 Entity 15 polymer

Description description	DNA template (40-MER)
Molecular weight formula_weight	14659.4 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	T

CACTCTACCGATAAGCAGACGTACCTCTCGACCCTGTGCTAGACACGG

Entity 16 Entity 16 polymer

Description description	;5'-3' exoribonuclease 2 ;
Molecular weight formula_weight	102208.3 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	X

MGVPALFRLLSRKFAKVITPVIEAPTEKLPDGTEIEPDLSLPNPNGVECDNLYLDMNGIVHPCSHPEDRPAPETEDEMMVAVFEYTDRILAMVRPRQLLFIAIDGVAPRAKMNQQRSRRFRSSREAALKEEELQAFIEEAKQQGIPIDENATKKKSWDSNCITPGTPFMDTLAKSLRYYIINKLNSDPCWRNVRFILSDASVPGEGEHKIMEFIRSQRVKPEYDPNTHHVVYGLDAALIMLGLATHEPHFRVLREDVFFQQGSTKKTKEERLGIKRLDDVSETNKVPVKKPFIWLNVSILREYLEVELYVPNLPFPFDLERAIDDWVFFIFFVGNDFLPHLPSLDIRDGAVERLTEIWRASLPHMGGYLTLDGSVNLARAEVILSAVGNQEDDIFKRLKQQEDRRNENYRRRQQRESNQESESYVDNVVIQRSVETQSTEVVTSSKSTSVDTKPPKKTQKIDAPAPVDLVNLSEKTSNRSLGATNRELINNRAANRLGLSREAAAVSSVNKLAASALKAQLVSNETLQNVPLEDSIASSSAYEDTDSIESSTPVVHPIDTKVSNVGQKRKAPDSTEENENTDTVRLYEPGYRERYYEQKFHISPDEPEKIREAVKHYVHGLCWVLLYYYQGCPSWTWYYPYHYAPFAADFKDLASIDVKFELNQPFKPYEQLLGVLPAASKNNLPEKLQTLMTDENSEIIDFYPENFTIDLNGKKFEWQGVALLPFIDENRLLNAVSKIYPQLTEEESKRNEDGSTLLFISEHHPMFSELVKQLYSKKRQGKPLKLSGKMAHGLFGKVNTNDSVIPNVSVQCPIDVTSADALQKYGSIDDNQSISLVFEVPKSHFVHKSMLLRGVKMPNRVLTPEDINQVRAERSFSSRRNNGNSGSGHHHHHHHH

Source Source

Source organism organism	Schizosaccharomyces pombe
Common name common_name	fission yeast
NCBI taxonomy ID ncbi_taxonomy_id	4896
Host organism host_organism	Escherichia coli

Entity 17 Entity 17 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	1

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	2.67 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	215000

Structure of s. pombe RNA polymerase II in complex with DSIF and Rat1/Rai1

1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase II subunit rpb1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit rpb7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB9,RBP9

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB11

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer