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6awf

Escherichia coli quinol:fumarate reductase crystallized without dicarboxylate

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Fumarate reductase flavoprotein subunit

Escherichia coli

UniProt P00363

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 Fumarate reductase iron-sulfur subunit × 1 （P0AC49） Fumarate reductase subunit C × 1 （B7MKV9） Fumarate reductase subunit D × 1 （B7MKV8） FLAVIN-ADENINE DINUCLEOTIDE × 1 PHOSPHATE ION × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 Fumarate reductase iron-sulfur subunit × 1 （P0AC49） Fumarate reductase subunit C × 1 （B7MKV9） Fumarate reductase subunit D × 1 （B7MKV8） FLAVIN-ADENINE DINUCLEOTIDE × 1 PHOSPHATE ION × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1kf6 1kf6 1kf6 1kfy 1kfy 1kfy 1l0v 1l0v 1l0v 3cir 3cir 4kx6

19 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FRDA_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–602； UniProt 1–602 Author chain E； PDBConstruct 1–602； UniProt 1–602

Fumarate reductase iron-sulfur subunit

Escherichia coli

UniProt P0AC49

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 Fumarate reductase flavoprotein subunit × 1 （P00363） Fumarate reductase subunit C × 1 （B7MKV9） Fumarate reductase subunit D × 1 （B7MKV8） FLAVIN-ADENINE DINUCLEOTIDE × 1 PHOSPHATE ION × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 Fumarate reductase flavoprotein subunit × 1 （P00363） Fumarate reductase subunit C × 1 （B7MKV9） Fumarate reductase subunit D × 1 （B7MKV8） FLAVIN-ADENINE DINUCLEOTIDE × 1 PHOSPHATE ION × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
6awf 6awf

2 assemblies

View Construct and Data Evidence
UniProt name FRDB_ECO57
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–243； UniProt 2–244 Author chain F； PDBConstruct 1–243； UniProt 2–244

Fumarate reductase subunit C

Escherichia coli

UniProt B7MKV9

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 Fumarate reductase flavoprotein subunit × 1 （P00363） Fumarate reductase iron-sulfur subunit × 1 （P0AC49） Fumarate reductase subunit D × 1 （B7MKV8） FLAVIN-ADENINE DINUCLEOTIDE × 1 PHOSPHATE ION × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 Fumarate reductase flavoprotein subunit × 1 （P00363） Fumarate reductase iron-sulfur subunit × 1 （P0AC49） Fumarate reductase subunit D × 1 （B7MKV8） FLAVIN-ADENINE DINUCLEOTIDE × 1 PHOSPHATE ION × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
6awf 6awf

2 assemblies

View Construct and Data Evidence
UniProt name FRDC_ECO45
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–130； UniProt 2–131 Author chain G； PDBConstruct 1–130； UniProt 2–131

Fumarate reductase subunit D

Escherichia coli

UniProt B7MKV8

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 Fumarate reductase flavoprotein subunit × 1 （P00363） Fumarate reductase iron-sulfur subunit × 1 （P0AC49） Fumarate reductase subunit C × 1 （B7MKV9） FLAVIN-ADENINE DINUCLEOTIDE × 1 PHOSPHATE ION × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 Fumarate reductase flavoprotein subunit × 1 （P00363） Fumarate reductase iron-sulfur subunit × 1 （P0AC49） Fumarate reductase subunit C × 1 （B7MKV9） FLAVIN-ADENINE DINUCLEOTIDE × 1 PHOSPHATE ION × 1 FE2/S2 (INORGANIC) CLUSTER × 1 FE3-S4 CLUSTER × 1 IRON/SULFUR CLUSTER × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
6awf 6awf

2 assemblies

View Construct and Data Evidence
UniProt name FRDD_ECO45
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 1–119； UniProt 1–119 Author chain H； PDBConstruct 1–119； UniProt 1–119

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id6awf
Deposition date deposition_date2017-09-05
Structure title titleEscherichia coli quinol:fumarate reductase crystallized without dicarboxylate
Keywords keywords;Complex II, quinol:fumarate reductase, fumarate reductase, succinate dehydrogenase, succinate oxidase, succinate:quinone oxidoreductase, FAD, flavoprotein, flavoenzyme, ELECTRON TRANSPORT ;; ELECTRON TRANSPORT
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

6awf__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

6awf__assembly_2__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

6awf__assembly_2__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）32.04 Å
Rg (electron density)32.87 Å
Total Rg32.86 Å
Atom count7536
Residues973
Excluded volume134490 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 6awf__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 6awf__assembly_2__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (9)

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6. Fold Classification (SCOP + CATH) 12 domains

CATH v4.4 (12 domains)

Domain ID domain_id6awfA01
Class class3 — Alpha Beta
Architecture architecture50 — 3-Layer(bba) Sandwich
Topology topology50 — FAD/NAD(P)-binding domain
Homologous superfamily homologous superfamily60 — FAD/NAD(P)-binding domain
Domain ID domain_id6awfA02
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology700 — Flavocytochrome C3; Chain A, domain 1
Homologous superfamily homologous superfamily10 — Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain
Domain ID domain_id6awfA03
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily100 — Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain
Domain ID domain_id6awfA04
Class class4 — Few Secondary Structures
Architecture architecture10 — Irregular
Topology topology80 — Rhinovirus 14, subunit 4
Homologous superfamily homologous superfamily40 — succinate dehydrogenase protein domain
Domain ID domain_id6awfB01
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily30 — Beta-grasp domain
Domain ID domain_id6awfB02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1060 — Fumarate Reductase Iron-sulfur Protein; Chain B, domain 2
Homologous superfamily homologous superfamily10 — Alpha-helical ferredoxin
Domain ID domain_id6awfC00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1300 — 3 helical TM bundles of succinate and fumarate reductases
Homologous superfamily homologous superfamily10 — Fumarate reductase/succinate dehydrogenase, transmembrane subunit
Domain ID domain_id6awfD00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1300 — 3 helical TM bundles of succinate and fumarate reductases
Homologous superfamily homologous superfamily10 — Fumarate reductase/succinate dehydrogenase, transmembrane subunit
Domain ID domain_id6awfF01
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily30 — Beta-grasp domain
Domain ID domain_id6awfF02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1060 — Fumarate Reductase Iron-sulfur Protein; Chain B, domain 2
Homologous superfamily homologous superfamily10 — Alpha-helical ferredoxin
Domain ID domain_id6awfG00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1300 — 3 helical TM bundles of succinate and fumarate reductases
Homologous superfamily homologous superfamily10 — Fumarate reductase/succinate dehydrogenase, transmembrane subunit
Domain ID domain_id6awfH00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1300 — 3 helical TM bundles of succinate and fumarate reductases
Homologous superfamily homologous superfamily10 — Fumarate reductase/succinate dehydrogenase, transmembrane subunit
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7. Citations (1)