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6jeh

Crystal structure of calcium free human gelsolin amyloid mutant D187Y

Method: X-RAY DIFFRACTION Dmax: 124.5 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Gelsolin

Homo sapiens

UniProt P06396

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 56–782 Chain B； UniProt 56–782 Mutation：D187Y No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION；pH 8.5；298 K；15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl pH8.5 Resolution 2.95 Å R-free 0.249

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6JEH Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1C0F CRYSTAL STRUCTURE OF DICTYOSTELIUM CAATP-ACTIN IN COMPLEX WITH GELSOLIN SEGMENT 1 Deposited 1999-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 53–176（124 aa）
Mutation：N35C CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；290 K；IMIDAZOLE, SODIUM CHLORIDE, ATP, CALCIUM CHLORIDE, MAGNESIUM CHLORIDE, SODIUM AZIDE, DITHIOTHREITOL, PEG 6000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution 2.40 Å R-free 0.231
1C0G CRYSTAL STRUCTURE OF 1:1 COMPLEX BETWEEN GELSOLIN SEGMENT 1 AND A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 228: Q228K/T229A/A230Y/E360H) Deposited 1999-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 53–176（124 aa）
Mutation：YES CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；MES, SODIUM CHLORIDE, ATP, CALCIUM CHLORIDE, MAGNESIUM CHLORIDE, SODIUM AZIDE, DITHIOTHREITOL, PEG 6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.00 Å R-free 0.223
1D4X Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin Segment 1 at 1.75 A resolution. Deposited 1999-10-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–177（126 aa） Fragment：SEGMENT 1
Not recorded MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SO2 SULFUR DIOXIDE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100 MM TRIS-HCL, PH 8.0, 2 MM MG-ATP, 1.60 M LI2SO4,, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.75 Å R-free 0.233
1D4X Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin Segment 1 at 1.75 A resolution. Deposited 1999-10-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 52–177（126 aa） Fragment：SEGMENT 1
Not recorded MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SO2 SULFUR DIOXIDE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100 MM TRIS-HCL, PH 8.0, 2 MM MG-ATP, 1.60 M LI2SO4,, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.75 Å R-free 0.233
1DEJ CRYSTAL STRUCTURE OF A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 646: Q228K/T229A/A230Y/A231K/S232E/E360H) IN COMPLEX WITH HUMAN GELSOLIN SEGMENT 1 Deposited 1999-11-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 53–176（124 aa） Fragment：SEGMENT 1
Mutation：YES CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；MES, NACL, CACL2, MGCL2, ATP, PEG6000, DTT, NAN3, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.40 Å R-free 0.214
1EQY COMPLEX BETWEEN RABBIT MUSCLE ALPHA-ACTIN: HUMAN GELSOLIN DOMAIN 1 Deposited 2000-04-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 52–176（125 aa） Fragment：DOMAIN 1
Mutation：N33C CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, Vapor Diffusion, temperature 298.0K
Resolution 2.30 Å R-free 0.280
1ESV COMPLEX BETWEEN LATRUNCULIN A:RABBIT MUSCLE ALPHA ACTIN:HUMAN GELSOLIN DOMAIN 1 Deposited 2000-04-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 52–176（125 aa） Fragment：DOMAIN 1
Mutation：N33C CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium Chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, VAPOR DIFFUSION, temperature 298.0K
Resolution 2.00 Å R-free 0.286
1H1V gelsolin G4-G6/actin complex Deposited 2002-07-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 439–769（331 aa） Fragment：G4-G6, RESIDUES 412-742 OF CYTOPLASMIC ISOFORM
Not recorded CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；PROTEIN WAS CRYSTALLIZED FROM 100MM HEPES BUFFER, PH 7.5, 20% GLYCEROL, 10 % PEG 8000
Resolution 2.99 Å R-free 0.268
1KCQ Human Gelsolin Domain 2 with a Cd2+ bound Deposited 2001-11-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 185–288（104 aa） Fragment：DOMAIN 2
Not recorded CD CADMIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；290 K；PEG 400, cadmium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 290K
Resolution 1.65 Å R-free 0.233
1KCQ Human Gelsolin Domain 2 with a Cd2+ bound Deposited 2001-11-09 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 185–288（104 aa） Fragment：DOMAIN 2
Not recorded CD CADMIUM ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；290 K；PEG 400, cadmium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 290K
Resolution 1.65 Å R-free 0.233
1MDU Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1 (GS-1) Deposited 2002-08-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 52–176（125 aa） Fragment：actin-severing
Not recorded CA CALCIUM ION × 12 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；PEG 3350, tris, calcium chloride, magnesium chloride, ADP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.20 Å R-free 0.233
1MDU Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1 (GS-1) Deposited 2002-08-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 52–176（125 aa） Fragment：actin-severing
Not recorded CA CALCIUM ION × 9 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；PEG 3350, tris, calcium chloride, magnesium chloride, ADP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.20 Å R-free 0.233
1NLV Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human Gelsolin Segment 1 Deposited 2003-01-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–176（125 aa） Fragment：DOMAIN 1
Not recorded CA CALCIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SO2 SULFUR DIOXIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；lithium chloride, magnesium chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 1.80 Å R-free 0.258
1NLV Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human Gelsolin Segment 1 Deposited 2003-01-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 52–176（125 aa） Fragment：DOMAIN 1
Not recorded CA CALCIUM ION × 4 SO4 SULFATE ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SO2 SULFUR DIOXIDE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；lithium chloride, magnesium chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 1.80 Å R-free 0.258
1NM1 Crystal Structure of D. Dicsoideum Actin Complexed With Gelsolin Segment 1 and Mg ATP at 1.8 A Resolution Deposited 2003-01-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–176（125 aa） Fragment：DOMAIN 1
Not recorded MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SO2 SULFUR DIOXIDE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；Magnesium chloride, litheim chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 1.80 Å R-free 0.232
1NM1 Crystal Structure of D. Dicsoideum Actin Complexed With Gelsolin Segment 1 and Mg ATP at 1.8 A Resolution Deposited 2003-01-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 52–176（125 aa） Fragment：DOMAIN 1
Not recorded MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SO2 SULFUR DIOXIDE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；Magnesium chloride, litheim chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 1.80 Å R-free 0.232
1NMD Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP Deposited 2003-01-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–176（125 aa） Fragment：domain I
Not recorded SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SO2 SULFUR DIOXIDE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；300 K；Lithium sulfate, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
Resolution 1.90 Å R-free 0.250
1NMD Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP Deposited 2003-01-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 52–176（125 aa） Fragment：domain I
Not recorded SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 SO2 SULFUR DIOXIDE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；300 K；Lithium sulfate, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
Resolution 1.90 Å R-free 0.250
1P8X The Calcium-Activated C-terminal half of gelsolin Deposited 2003-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 439–782（344 aa） Fragment：C-terminal domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277.15 K；15% (w/v) PEG 8000, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K
Resolution 2.00 Å R-free 0.245
1P8X The Calcium-Activated C-terminal half of gelsolin Deposited 2003-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 439–782（344 aa） Fragment：C-terminal domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277.15 K；15% (w/v) PEG 8000, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K
Resolution 2.00 Å R-free 0.245
1P8X The Calcium-Activated C-terminal half of gelsolin Deposited 2003-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 439–782（344 aa） Fragment：C-terminal domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277.15 K；15% (w/v) PEG 8000, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K
Resolution 2.00 Å R-free 0.245
1P8Z Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156 Deposited 2003-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–187（136 aa）
Not recorded CD CADMIUM ION × 3 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.263
1P8Z Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156 Deposited 2003-05-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 52–187（136 aa）
Not recorded CD CADMIUM ION × 6 CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.263
1SOL A PIP2 AND F-ACTIN-BINDING SITE OF GELSOLIN, RESIDUE 150-169 (NMR, AVERAGED STRUCTURE) Deposited 1995-09-29 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 177–196（20 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR mmCIF provides none of the parsed conditions Resolution not provided
1T44 Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation Deposited 2004-04-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 55–179（125 aa） Fragment：Chimera of Gelsolin domain 1 (residues 28-152) from human and C-Terminal domain of thymosin Beta-4 from mouse (residues 153-171)
Not recorded CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.5；293 K；PEG 8000, Sodium acetate, calcium chloride, pH 6.5, microbatch, temperature 293K
Resolution 2.00 Å R-free 0.195
1YAG STRUCTURE OF THE YEAST ACTIN-HUMAN GELSOLIN SEGMENT 1 COMPLEX Deposited 1998-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–176（125 aa） Fragment：SUBDOMAIN 1
Not recorded MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.50
Resolution 1.90 Å R-free 0.231
1YAG STRUCTURE OF THE YEAST ACTIN-HUMAN GELSOLIN SEGMENT 1 COMPLEX Deposited 1998-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 52–176（125 aa） Fragment：SUBDOMAIN 1
Not recorded MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.50
Resolution 1.90 Å R-free 0.231
1YVN THE YEAST ACTIN VAL 159 ASN MUTANT COMPLEX WITH HUMAN GELSOLIN SEGMENT 1. Deposited 1999-03-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–176（125 aa） Fragment：FRAGMENT 1
Not recorded MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8；0.1 M HEPES, 1.60 M (NH4)2SO4, 2 MM MG-ATP, PH=8.0.
Resolution 2.10 Å R-free 0.235
1YVN THE YEAST ACTIN VAL 159 ASN MUTANT COMPLEX WITH HUMAN GELSOLIN SEGMENT 1. Deposited 1999-03-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 52–176（125 aa） Fragment：FRAGMENT 1
Not recorded MG MAGNESIUM ION × 2 SO4 SULFATE ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8；0.1 M HEPES, 1.60 M (NH4)2SO4, 2 MM MG-ATP, PH=8.0.
Resolution 2.10 Å R-free 0.235
2FF3 Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin Deposited 2005-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 52–179（128 aa） Fragment：Gelsolin domain 1
Not recorded CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.5；293.15 K；5% PEG 8000, 0.1M sodium acetate, 10mM calcium chloride, pH 6.5, microbatch, temperature 293.15K
Resolution 2.00 Å R-free 0.250
2FF6 Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin Deposited 2005-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain G 51–178（128 aa） Fragment：Gelsolin domain 1
Not recorded CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；7% PEG 3000, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
Resolution 2.05 Å R-free 0.237
2FH1 C-terminal half of gelsolin soaked in low calcium at pH 4.5 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 439–782（344 aa） Fragment：C-terminal half domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 1.55 Å R-free 0.214
2FH1 C-terminal half of gelsolin soaked in low calcium at pH 4.5 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 439–782（344 aa） Fragment：C-terminal half domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 1.55 Å R-free 0.214
2FH1 C-terminal half of gelsolin soaked in low calcium at pH 4.5 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 439–782（344 aa） Fragment：C-terminal half domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 1.55 Å R-free 0.214
2FH2 C-terminal half of gelsolin soaked in EGTA at pH 4.5 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 439–782（344 aa） Fragment：C-terminal half domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 2.50 Å R-free 0.255
2FH2 C-terminal half of gelsolin soaked in EGTA at pH 4.5 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 439–782（344 aa） Fragment：C-terminal half domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 2.50 Å R-free 0.255
2FH2 C-terminal half of gelsolin soaked in EGTA at pH 4.5 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 439–782（344 aa） Fragment：C-terminal half domain
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 2.50 Å R-free 0.255
2FH3 C-terminal half of gelsolin soaked in low calcium at pH 8 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 439–782（344 aa） Fragment：C-terminal half domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 2.87 Å R-free 0.262
2FH3 C-terminal half of gelsolin soaked in low calcium at pH 8 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 439–782（344 aa） Fragment：C-terminal half domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 2.87 Å R-free 0.262
2FH3 C-terminal half of gelsolin soaked in low calcium at pH 8 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 439–782（344 aa） Fragment：C-terminal half domain
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 2.87 Å R-free 0.262
2FH4 C-terminal half of gelsolin soaked in EGTA at pH 8 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 439–782（344 aa） Fragment：C-terminal half domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 3.00 Å R-free 0.310
2FH4 C-terminal half of gelsolin soaked in EGTA at pH 8 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 439–782（344 aa） Fragment：C-terminal half domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 3.00 Å R-free 0.310
2FH4 C-terminal half of gelsolin soaked in EGTA at pH 8 Deposited 2005-12-23 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 439–782（344 aa） Fragment：C-terminal half domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions micro batch under oil；pH 4.5；277 K；PEG 8000, pH 4.5, micro batch under oil, temperature 277K
Resolution 3.00 Å R-free 0.310
3A5L Crystal Structure of a Dictyostelium P109A Mg2+-Actin in Complex with Human Gelsolin Segment 1 Deposited 2009-08-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 53–176（124 aa） Fragment：GELSOLIN-LIKE 1, residues 53-176
Mutation：N35C CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.40 Å R-free 0.207
3A5M Crystal Structure of a Dictyostelium P109I Mg2+-Actin in Complex with Human Gelsolin Segment 1 Deposited 2009-08-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 53–176（124 aa） Fragment：GELSOLIN-LIKE 1, residues 53-176
Mutation：N35C CA CALCIUM ION × 2 MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.40 Å R-free 0.223
3A5N Crystal Structure of a Dictyostelium P109A Ca2+-Actin in Complex with Human Gelsolin Segment 1 Deposited 2009-08-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 53–176（124 aa） Fragment：GELSOLIN-LIKE 1, residues 53-176
Mutation：N35C CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.36 Å R-free 0.204
3A5O Crystal Structure of a Dictyostelium P109I Ca2+-Actin in Complex with Human Gelsolin Segment 1 Deposited 2009-08-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 53–176（124 aa） Fragment：GELSOLIN-LIKE 1, residues 53-176
Mutation：N35C CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.40 Å R-free 0.207
3CI5 Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin Deposited 2008-03-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–176（125 aa） Fragment：Gelsolin Segment 1 (UNP residues 52-176)
Not recorded MG MAGNESIUM ION × 1 SO4 SULFATE ION × 10 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 5 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M HEPES, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 10% Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.70 Å R-free 0.191
3CI5 Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin Deposited 2008-03-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 52–176（125 aa） Fragment：Gelsolin Segment 1 (UNP residues 52-176)
Not recorded MG MAGNESIUM ION × 2 SO4 SULFATE ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GOL GLYCEROL × 10 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M HEPES, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 10% Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.70 Å R-free 0.191
3CIP Complex of Dictyostelium Discoideum Actin with Gelsolin Deposited 2008-03-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–176（125 aa） Fragment：Gelsolin Segment 1 (UNP RESIDUES 52-176)
Not recorded MG MAGNESIUM ION × 1 SO4 SULFATE ION × 9 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 3 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M HEPES, pH7.5, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 5% Glycerol, vapor diffusion, hanging drop, temperature 293K
Resolution 1.60 Å R-free 0.197
3CIP Complex of Dictyostelium Discoideum Actin with Gelsolin Deposited 2008-03-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 52–176（125 aa） Fragment：Gelsolin Segment 1 (UNP RESIDUES 52-176)
Not recorded MG MAGNESIUM ION × 2 SO4 SULFATE ION × 18 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GOL GLYCEROL × 6 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M HEPES, pH7.5, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 5% Glycerol, vapor diffusion, hanging drop, temperature 293K
Resolution 1.60 Å R-free 0.197
3CJB Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1 Deposited 2008-03-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 52–176（125 aa） Fragment：segment 1
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10% Peg MME 5000, 5% Tacsimate, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.21 Å R-free 0.269
3CJC Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with DNase I and Gelsolin-segment 1 Deposited 2008-03-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain G 52–176（125 aa） Fragment：segment 1
Not recorded CA CALCIUM ION × 2 SO4 SULFATE ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M (NH4)SO4, 0.1 M Bis-Tris, 0.1 M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.90 Å R-free 0.278
3FFK Crystal structure of human Gelsolin domains G1-G3 bound to Actin Deposited 2008-12-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 52–426（375 aa） Fragment：UNP residues 52-426
Not recorded CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Resolution 3.00 Å R-free 0.273
3FFK Crystal structure of human Gelsolin domains G1-G3 bound to Actin Deposited 2008-12-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 52–426（375 aa） Fragment：UNP residues 52-426
Not recorded CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Resolution 3.00 Å R-free 0.273
3FFN Crystal structure of calcium-free human gelsolin Deposited 2008-12-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–782（782 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；297 K；15% glycerol, 100 mM Bis-Tris HCl, 1.5M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Resolution 3.00 Å R-free 0.271
3FFN Crystal structure of calcium-free human gelsolin Deposited 2008-12-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–782（782 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；297 K；15% glycerol, 100 mM Bis-Tris HCl, 1.5M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Resolution 3.00 Å R-free 0.271
3TU5 Actin complex with Gelsolin Segment 1 fused to Cobl segment Deposited 2011-09-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 53–174（122 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；10% (w/v) polyethyleneglycol 20000, 2% (v/v) dioxane, and 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.00 Å R-free 0.204
4PKG Complex of ATP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 12–136（125 aa） Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
Resolution 1.80 Å R-free 0.187
4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 12–136（125 aa） Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
Resolution 2.15 Å R-free 0.326
4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 12–136（125 aa） Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
Resolution 2.15 Å R-free 0.326
4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 12–136（125 aa） Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
Resolution 2.15 Å R-free 0.326
4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain J 12–136（125 aa） Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 1 (UNP residues 50-101)
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
Resolution 2.15 Å R-free 0.326
4PKI Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 12–136（125 aa） Fragment：Gelsolin (UNP residues 12-136), GGSGGSGGS linker, Tmod1 Actin-binding site 2 (UNP residues 160-349)
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.25 M sodium chloride, 12% w/v PEG3350
Resolution 2.30 Å R-free 0.201
4S10 Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis Deposited 2015-01-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 186–288（103 aa） Fragment：GELSOLIN DOMAIN 2
Mutation：S226D, R228D CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；288 K；30% PEG 1500, VAPOR DIFFUSION, temperature 288K
Resolution 2.61 Å R-free 0.312
4S10 Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis Deposited 2015-01-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 186–288（103 aa） Fragment：GELSOLIN DOMAIN 2
Mutation：S226D, R228D CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；288 K；30% PEG 1500, VAPOR DIFFUSION, temperature 288K
Resolution 2.61 Å R-free 0.312
4Z94 Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2 Deposited 2015-04-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 12–136（125 aa） Fragment：;Gelsolin residues 12-136 (UNP), linker,Tropomodulin-1 residues 160-228 (UNP), Leiomodin-1 Actin-Binding Site 2 (UNP residues 364-486) ;
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.8；291.5 K；20% PEG3350, 200 mM lithium sulfate, 100 mM Tris, pH 8.8, 15% glycerol
Resolution 2.40 Å R-free 0.243
5FAE N184K pathological variant of gelsolin domain 2 (trigonal form) Deposited 2015-12-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 178–293（116 aa） Fragment：UNP residues 178-293
Mutation：N184K CA CALCIUM ION × 1 SO4 SULFATE ION × 4 PEG DI(HYDROXYETHYL)ETHER × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.2 M lithium sulfate 0.1 M tris 40% PEG 400
Resolution 1.70 Å R-free 0.225
5FAF N184K pathological variant of gelsolin domain 2 (orthorhombic form) Deposited 2015-12-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 178–293（116 aa） Fragment：UNP residues 178-293
Mutation：N184K CA CALCIUM ION × 2 GOL GLYCEROL × 3 CL CHLORIDE ION × 2 ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；20% PEG 8000 0.1 M MES 0.2 M calcium acetate
Resolution 1.05 Å R-free 0.168
5H3M Solution structure of human Gelsolin protein domain 1 at pH 5.0 Deposited 2016-10-26 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 55–187（133 aa） Fragment：UNP residues 55-187
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5；298 K；Ionic strength (raw mmCIF value) 0；Pressure 1
NMR sample composition 0.65 mM 13C, 15N gelsolin domain 1, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
5H3N Solution structure of human Gelsolin protein domain 1 at pH 7.3 Deposited 2016-10-26 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 55–187（133 aa） Fragment：UNP residues 55-187
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.3；298 K；Ionic strength (raw mmCIF value) 0；Pressure 1
NMR sample composition 0.70 mM 13C, 15N human Gelsolin, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
5O2Z Domain swap dimer of the G167R variant of gelsolin second domain Deposited 2017-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 178–293（116 aa）
Chain B 178–293（116 aa）
Mutation：G167R Mutation：G167R CA CALCIUM ION × 2 FLC CITRATE ANION × 2 ACT ACETATE ION × 2 NA SODIUM ION × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；293 K；0.2M ammonium acetate, 0.1M sodium citrate, 30% PEG 4000
Resolution 1.70 Å R-free 0.242
5UBO Mical-oxidized Actin complex with Gelsolin Segment 1 Deposited 2016-12-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 12–138（127 aa） Fragment：unp residues 12-138
Not recorded CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；298 K；PEG 6000, NaCl, imidazole, ATP, calcium
Resolution 2.39 Å R-free 0.191
5ZZ0 HUMAN GELSOLIN FROM RESIDUES GLU28 TO ARG161 WITH CALCIUM Deposited 2018-05-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain G 55–188（134 aa） Fragment：UNP residues 55-188
Not recorded CA CALCIUM ION × 2 PG0 2-(2-METHOXYETHOXY)ETHANOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；Peg 6000 (20%), 100mM Sodium acetate, 200mM Calcium Chloride Dihydrate, pH 5
Resolution 2.63 Å R-free 0.254
5ZZ0 HUMAN GELSOLIN FROM RESIDUES GLU28 TO ARG161 WITH CALCIUM Deposited 2018-05-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 55–188（134 aa） Fragment：UNP residues 55-188
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；Peg 6000 (20%), 100mM Sodium acetate, 200mM Calcium Chloride Dihydrate, pH 5
Resolution 2.63 Å R-free 0.254
6H1F Structure of the nanobody-stabilized gelsolin D187N variant (second domain) Deposited 2018-07-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 178–293（116 aa）
Mutation：D187N SCN THIOCYANATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；0.1 M Potassium thiocyanate, 30%w/v PEG 2000 MME
Resolution 1.90 Å R-free 0.233
6JCO Crystal structure of calcium free human gelsolin amyloid mutant D187N Deposited 2019-01-29 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 56–782（727 aa）
Chain B 56–782（727 aa）
Mutation：D187N Mutation：D187N No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；298.1 K；15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl pH8.5
Resolution 2.88 Å R-free 0.267
6JEG Crystal structure of calcium free human gelsolin amyloid mutant G167R Deposited 2019-02-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 54–782（729 aa）
Chain B 54–782（729 aa）
Mutation：G167R Mutation：G167R GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；298 K；15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl, pH8.5
Resolution 2.98 Å R-free 0.237
6LJE Crystal structure of gelsolin G3 domain (calcium and magnesium condition) Deposited 2019-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 297–397（101 aa） Fragment：UNP residues 297-397
Not recorded GOL GLYCEROL × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG3350, ammonium sulphate, calcium chloride, magnesium chloride
Resolution 1.40 Å R-free 0.189
6LJE Crystal structure of gelsolin G3 domain (calcium and magnesium condition) Deposited 2019-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 297–397（101 aa） Fragment：UNP residues 297-397
Not recorded GOL GLYCEROL × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG3350, ammonium sulphate, calcium chloride, magnesium chloride
Resolution 1.40 Å R-free 0.189
6LJF Crystal structure of gelsolin G3 domain (calcium condition) Deposited 2019-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 297–397（101 aa） Fragment：UNP residues 297-397
Not recorded CA CALCIUM ION × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG3350, ammonium sulphate, calcium chloride
Resolution 1.50 Å R-free 0.183
6LJF Crystal structure of gelsolin G3 domain (calcium condition) Deposited 2019-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 297–397（101 aa） Fragment：UNP residues 297-397
Not recorded CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG3350, ammonium sulphate, calcium chloride
Resolution 1.50 Å R-free 0.183
6Q9R Crystal structure of the pathological N184K variant of calcium-free human gelsolin Deposited 2018-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 28–782（755 aa）
Mutation：N184K CL CHLORIDE ION × 6 GOL GLYCEROL × 8 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1.5 M ammonium sulfate, 15 % glycerol, 100 mM Tris-HCl
Resolution 2.73 Å R-free 0.260
6Q9R Crystal structure of the pathological N184K variant of calcium-free human gelsolin Deposited 2018-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 28–782（755 aa）
Mutation：N184K CL CHLORIDE ION × 3 GOL GLYCEROL × 9 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1.5 M ammonium sulfate, 15 % glycerol, 100 mM Tris-HCl
Resolution 2.73 Å R-free 0.260
6Q9Z Crystal structure of the pathological G167R variant of calcium-free human gelsolin, Deposited 2018-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 28–782（755 aa）
Mutation：G167R SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；1.5 M ammonium sulfate, 20% glycerol, 100 mM Tris-HCl
Resolution 3.80 Å R-free 0.264
6Q9Z Crystal structure of the pathological G167R variant of calcium-free human gelsolin, Deposited 2018-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 28–782（755 aa）
Mutation：G167R SO4 SULFATE ION × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；1.5 M ammonium sulfate, 20% glycerol, 100 mM Tris-HCl
Resolution 3.80 Å R-free 0.264
6QBF Crystal structure of the pathological D187N variant of calcium-free human gelsolin. Deposited 2018-12-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 28–782（755 aa）
Mutation：D187N SO4 SULFATE ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1.3 M Ammonium sulfate, 19% glycerol, 100 mM Tris HCl
Resolution 3.50 Å R-free 0.276
6QBF Crystal structure of the pathological D187N variant of calcium-free human gelsolin. Deposited 2018-12-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 28–782（755 aa）
Mutation：D187N SO4 SULFATE ION × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；1.3 M Ammonium sulfate, 19% glycerol, 100 mM Tris HCl
Resolution 3.50 Å R-free 0.276
6QW3 Calcium-bound gelsolin domain 2 Deposited 2019-03-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 178–293（116 aa）
Not recorded CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；36% PEG5000 MME, 0.1M Sodium Acetate
Resolution 1.30 Å R-free 0.174
7P2B Crystal structure of human gelsolin amyloid mutant A551P Deposited 2021-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 28–782（755 aa）
Mutation：A551P SO4 SULFATE ION × 4 GOL GLYCEROL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；15% glycerol, 2.0 M ammonium sulfate, 0.1M tris HCl, pH8.5
Resolution 3.00 Å R-free 0.263
7P2B Crystal structure of human gelsolin amyloid mutant A551P Deposited 2021-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 28–782（755 aa）
Mutation：A551P SO4 SULFATE ION × 3 GOL GLYCEROL × 3 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；15% glycerol, 2.0 M ammonium sulfate, 0.1M tris HCl, pH8.5
Resolution 3.00 Å R-free 0.263
8VIZ Structure of full-length gelsolin bound to the barbed end of F-actin Deposited 2024-01-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 52–782（731 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 14 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
8VKH Structure of gelsolin domains G1G3 bound to the barbed end of F-actin Deposited 2024-01-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 52–782（731 aa）
Chain H 52–782（731 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 4 CA CALCIUM ION × 14 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.63 Å
9UG2 Severed and capped actin fragment by two G1G3 domains of gelsolin Deposited 2025-04-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 54–401（348 aa）
Chain B 54–401（348 aa）
Not recorded CA CALCIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å

60 other PDB entries and 94 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name GELS_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–727； UniProt 56–782 Author chain B； PDBConstruct 1–727； UniProt 56–782

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6jeh

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6jeh
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6jeh
Deposition date deposition_date2019-02-05
Structure title titleCrystal structure of calcium free human gelsolin amyloid mutant D187Y
Keywords keywordsgelsolin, amyloidosis, calcium activation, Actin Binding Protein; Actin Binding Protein
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier39.16 Å
Radius of gyration Rg (electron density) rg_electron38.91 Å
Forward intensity I(0) i0371828000.00
Molecular weight molecular_weight155830.0 kDa
Excluded volume excluded_volume194320 ų
Envelope volume envelope_volume258330 ų
Hydration-shell volume shell_volume55060 ų
Envelope diameter envelope_diameter129.8 Å
Shell Rg shell_rg44.98 Å
Envelope Rg envelope_rg38.37 Å
Shape Rg shape_rg38.89 Å
Total Rg total_rg39.31 Å
Total atoms total_atoms11005
Residues n_residues1408
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax124.5 Å
Rg (real space) rg_real39.07 Å
Rg uncertainty (real space) rg_real_error0.94 Å
I(0) (real space) i0_real3.7180e+08
I(0) uncertainty (real space) i0_real_error5.4950e+06
Rg (reciprocal space) rg_reciprocal39.13 Å
I(0) (reciprocal space) i0_reciprocal371800000.0000
Solution quality estimate total_estimate0.9047
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary43.6 Å
Skewness Skewness skewness0.212
Kurtosis Kurtosis kurtosis-0.628
Angular range angular_range— – 0.2000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha48520000.0000
Real-space data points n_real_points41
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.951; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.903
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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7. Fold Classification (SCOP + CATH) 22 domains

SCOP 2.08 (12 domains)

Domain ID domain_idd6jeha1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jeha2
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jeha3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jeha4
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jeha5
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jeha6
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jehb1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jehb2
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jehb3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jehb4
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jehb5
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like
Domain ID domain_idd6jehb6
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.1 — Gelsolin-like

CATH v4.4 (10 domains)

Domain ID domain_id6jehA01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
Domain ID domain_id6jehA03
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
Domain ID domain_id6jehA04
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
Domain ID domain_id6jehA05
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
Domain ID domain_id6jehA06
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
Domain ID domain_id6jehB01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
Domain ID domain_id6jehB03
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
Domain ID domain_id6jehB04
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
Domain ID domain_id6jehB05
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
Domain ID domain_id6jehB06
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
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8. Citations (1)

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9. Files and Curves (10)