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6wlg

Ints3 C-terminal Domain

Method: X-RAY DIFFRACTION Dmax: 108.0 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Integrator complex subunit 3

Homo sapiens

UniProt Q68E01

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 555–977 Chain B； UniProt 555–977 Not recorded No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；289 K；0.2 M Magnesium Acetate, 13% PEG 3350 Resolution 3.11 Å R-free 0.263

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6WLG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4OWT Structural basis of SOSS1 complex assembly Deposited 2014-02-03 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 35–498（464 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289.15 K；21% PEG2000 MME, 0.1M MES pH6.0, 0.05M sodium chloride
Resolution 2.00 Å R-free 0.195
4OWW Structural basis of SOSS1 in complex with a 35nt ssDNA Deposited 2014-02-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Heteromer；Protein × 3 PDB declaration: tetrameric
Chain A 1–500（500 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；20% PEG2000 MME, 0.1M MES pH6.0, 0.05M sodium chloride.
Resolution 2.30 Å R-free 0.218
4OWX Structural basis of SOSS1 in complex with a 12nt ssDNA Deposited 2014-02-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Heteromer；Protein × 2 PDB declaration: trimeric
Chain A 1–500（500 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；16% PEG3350, 0.1M MES pH6.0, 0.1M ammonium formate
Resolution 2.30 Å R-free 0.226
7BV7 INTS3 complexed with INTS6 Deposited 2020-04-09 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 559–994（436 aa）
Chain B 559–994（436 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；288 K；1.7 M Ammonium citrate pH 7.0 4% pentaerythritol ethoxylate
Resolution 2.40 Å R-free 0.249
8HPP Crystal structure of human INTS3 with SAGE1 Deposited 2022-12-12 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 573–1043（471 aa） Fragment：C-terminal motif
Chain B 573–1043（471 aa） Fragment：C-terminal motif
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；277 K；0.2 M Ammonium sulfate and 25% Ethylene Glycol 3350
Resolution 3.00 Å R-free 0.257
8RBZ Structure of Integrator-PP2A-SOSS-CTD post-termination complex Deposited 2023-12-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric
Chain c 1–1042（1042 aa）
Not recorded ZN ZINC ION × 2 MN MANGANESE (II) ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name INT3_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–423； UniProt 555–977 Author chain B； PDBConstruct 1–423； UniProt 555–977

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6wlg

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6wlg
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6wlg
Deposition date deposition_date2020-04-20
Structure title titleInts3 C-terminal Domain
Keywords keywordsDNA damage repair, snRNA processing, HEAT repeat, PROTEIN BINDING; PROTEIN BINDING
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier33.48 Å
Radius of gyration Rg (electron density) rg_electron32.69 Å
Forward intensity I(0) i0102755000.00
Molecular weight molecular_weight82110.0 kDa
Excluded volume excluded_volume103410 ų
Envelope volume envelope_volume138200 ų
Hydration-shell volume shell_volume36071 ų
Envelope diameter envelope_diameter110.4 Å
Shell Rg shell_rg38.32 Å
Envelope Rg envelope_rg32.97 Å
Shape Rg shape_rg32.68 Å
Total Rg total_rg33.18 Å
Total atoms total_atoms5750
Residues n_residues707
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax108.0 Å
Rg (real space) rg_real33.48 Å
Rg uncertainty (real space) rg_real_error0.88 Å
I(0) (real space) i0_real1.0280e+08
I(0) uncertainty (real space) i0_real_error1.6530e+06
Rg (reciprocal space) rg_reciprocal33.48 Å
I(0) (reciprocal space) i0_reciprocal102800000.0000
Solution quality estimate total_estimate0.8927
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary43.7 Å
Skewness Skewness skewness0.280
Kurtosis Kurtosis kurtosis-0.461
Angular range angular_range— – 0.2350 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha37190000.0000
Real-space data points n_real_points48
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.921; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.968; Smooth: 0.870
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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8. Citations (1)

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9. Files and Curves (10)