6y4o

Calmodulin bound to cardiac ryanodine receptor (RyR2) calmodulin binding domain

Method: X-RAY DIFFRACTION D_max: 58.8 Å Quality: GOOD

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Calmodulin-2

Homo sapiens

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain A； UniProt 1–149	Not recorded	Ryanodine receptor 2 × 1 （E9Q401） CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1 M Sodium Acetate pH 4.70 and 23 % PEG 550 MME	Resolution 1.84 Å R-free 0.208

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 6Y4O	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
6PLM Legionella pneumophila SidJ/ Calmodulin 2 complex Deposited 2019-07-01	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 2–148（147 aa）	Not recorded	CA CALCIUM ION × 3 POP PYROPHOSPHATE 2- × 1 AMP ADENOSINE MONOPHOSPHATE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.1 M Tris pH 9.2, 0.2 M Sodium Iodide , 15% PEG 3350, 1 mM Calcium Chloride, 1 mM ATP	Resolution 2.59 Å R-free 0.241
6PLM Legionella pneumophila SidJ/ Calmodulin 2 complex Deposited 2019-07-01	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 2–148（147 aa）	Not recorded	CA CALCIUM ION × 3 POP PYROPHOSPHATE 2- × 1 AMP ADENOSINE MONOPHOSPHATE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.1 M Tris pH 9.2, 0.2 M Sodium Iodide , 15% PEG 3350, 1 mM Calcium Chloride, 1 mM ATP	Resolution 2.59 Å R-free 0.241
6S5T Legionella pneumophila SidJ-Human calmodulin complex Deposited 2019-07-02	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–149（149 aa）	Not recorded	ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.15 Å
6SZ5 Human calmodulin bound to a peptide of human NADPH oxidase 5 Deposited 2019-10-02	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–149（149 aa）	Not recorded	CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.4；293 K；30 mM Tris-HCl at pH 7.4, 2.5 mM CaCl2 and 2.5% (v/v) glycerol	Resolution 2.23 Å R-free 0.294
6XXF 1.7 Angstrom crystal structure of Ca/CaM:RyR2 peptide complex Deposited 2020-01-27	Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain AAA 1–149（149 aa）	Not recorded	CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.1M sodium acetate pH4.5, 0.2M ammonium acetate, 10% PEG4000	Resolution 1.70 Å R-free 0.205
7MIR Cryo-EM structure of SidJ-SdeA-CaM reaction intermediate complex Deposited 2021-04-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain B 1–149（149 aa）	Not recorded	AMP ADENOSINE MONOPHOSPHATE × 1 MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 6.5；25 mM Bis-tris pH 6.5, 100 mM NaCl, 1 mM TCEP, 2 mM MgCl2, 1 mM ATP cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
7MIS Cryo-EM structure of SidJ-SdeC-CaM reaction intermediate complex Deposited 2021-04-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain B 1–149（149 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 NA SODIUM ION × 1 CA CALCIUM ION × 1 AMP ADENOSINE MONOPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 6.5；25 mM Bis-tris pH 6.5, 100 mM NaCl, 1 mM TCEP, 2 mM MgCl2, 1 mM ATP cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.80 Å
7PPO Structure of SidJ/CaM bound to SdeA in pre-glutamylation state Deposited 2021-09-14	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain B 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 1 CA CALCIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.91 Å
7PQE Structure of SidJ/CaM bound to SdeA in post-catalysis state Deposited 2021-09-17	Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain B 1–149（149 aa）	Not recorded	MG MAGNESIUM ION × 1 CA CALCIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8K9Z Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin Deposited 2023-08-02	Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–149（149 aa）	Not recorded	CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；17% (w/v) PEG 3350 and 0.18 M magnesium acetate	Resolution 2.90 Å R-free 0.304
8K9Z Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin Deposited 2023-08-02	Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 1–149（149 aa）	Not recorded	CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；17% (w/v) PEG 3350 and 0.18 M magnesium acetate	Resolution 2.90 Å R-free 0.304
8KA0 Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin and a nicotinamide adenine dinucleotide (NAD+) Deposited 2023-08-02	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–149（149 aa）	Not recorded	NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 CA CALCIUM ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M Tris-HCl (pH 8.5), 32.5% (w/v) PEG 4000 and 0.15 M sodium acetate. 10 mM NAD+ soaking	Resolution 2.35 Å R-free 0.263
8KA0 Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin and a nicotinamide adenine dinucleotide (NAD+) Deposited 2023-08-02	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 1–149（149 aa）	Not recorded	NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 CA CALCIUM ION × 2 GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M Tris-HCl (pH 8.5), 32.5% (w/v) PEG 4000 and 0.15 M sodium acetate. 10 mM NAD+ soaking	Resolution 2.35 Å R-free 0.263
8KA0 Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin and a nicotinamide adenine dinucleotide (NAD+) Deposited 2023-08-02	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain F 1–149（149 aa）	Not recorded	NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 CA CALCIUM ION × 2 GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M Tris-HCl (pH 8.5), 32.5% (w/v) PEG 4000 and 0.15 M sodium acetate. 10 mM NAD+ soaking	Resolution 2.35 Å R-free 0.263
8KA0 Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin and a nicotinamide adenine dinucleotide (NAD+) Deposited 2023-08-02	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain H 1–149（149 aa）	Not recorded	NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 CA CALCIUM ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M Tris-HCl (pH 8.5), 32.5% (w/v) PEG 4000 and 0.15 M sodium acetate. 10 mM NAD+ soaking	Resolution 2.35 Å R-free 0.263
8KA1 Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-free calmodulin Deposited 2023-08-02	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–149（149 aa）	Mutation：E124Q Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；20% (w/v) PEG 3350, 0.1 M HEPES-NaOH (pH 7.5), 0.28 M MgCl2, and 6 mM MnCl2	Resolution 2.82 Å R-free 0.342
8KA1 Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-free calmodulin Deposited 2023-08-02	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 1–149（149 aa）	Mutation：E124Q Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；20% (w/v) PEG 3350, 0.1 M HEPES-NaOH (pH 7.5), 0.28 M MgCl2, and 6 mM MnCl2	Resolution 2.82 Å R-free 0.342
8KA1 Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-free calmodulin Deposited 2023-08-02	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain F 1–149（149 aa）	Mutation：E124Q Non-standard monomer：Yes (specific site not provided by mmCIF)	MG MAGNESIUM ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；20% (w/v) PEG 3350, 0.1 M HEPES-NaOH (pH 7.5), 0.28 M MgCl2, and 6 mM MnCl2	Resolution 2.82 Å R-free 0.342
8KA1 Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-free calmodulin Deposited 2023-08-02	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain H 1–149（149 aa）	Mutation：E124Q Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；20% (w/v) PEG 3350, 0.1 M HEPES-NaOH (pH 7.5), 0.28 M MgCl2, and 6 mM MnCl2	Resolution 2.82 Å R-free 0.342

11 other PDB entries and 19 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	CALM2_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–149； UniProt 1–149

Ryanodine receptor 2

Mus musculus

UniProt E9Q401

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain B； UniProt 3580–3606	Not recorded	Calmodulin-2 × 1 （P0DP24） CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1 M Sodium Acetate pH 4.70 and 23 % PEG 550 MME	Resolution 1.84 Å R-free 0.208

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 6Y4O	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
2MC2 X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A Deposited 2013-08-13	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 10–224（215 aa） Fragment：domain A (UNP residues 10-224)	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 7；288 K；Ionic strength (raw mmCIF value) 0.328；Pressure ambient NMR sample composition 0.4 mM [U-13C; U-15N; U-2H] RyR2, 20 mM sodium phosphate, 300 mM sodium chloride, 2 mM TCEP, 5 mM DTT, 90% H2O/10% D2O \| 90% H2O/10% D2O	Resolution not provided
4ETV Crystal structure of mouse ryanodine receptor 2 (2699-2904) Deposited 2012-04-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2699–2904（206 aa）	Mutation：K2879A Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；295 K；9% PEG3350, 0.1M Bicine, pH 9, vapor diffusion, hanging drop, temperature 295K	Resolution 1.65 Å R-free 0.250
4ETV Crystal structure of mouse ryanodine receptor 2 (2699-2904) Deposited 2012-04-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 2699–2904（206 aa）	Mutation：K2879A Non-standard monomer：Yes (specific site not provided by mmCIF)	CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；295 K；9% PEG3350, 0.1M Bicine, pH 9, vapor diffusion, hanging drop, temperature 295K	Resolution 1.65 Å R-free 0.250
4ETV Crystal structure of mouse ryanodine receptor 2 (2699-2904) Deposited 2012-04-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2699–2904（206 aa） Chain B 2699–2904（206 aa）	Mutation：K2879A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：K2879A Non-standard monomer：Yes (specific site not provided by mmCIF)	CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；295 K；9% PEG3350, 0.1M Bicine, pH 9, vapor diffusion, hanging drop, temperature 295K	Resolution 1.65 Å R-free 0.250
4KEI Crystal structure of mouse Ryanodine Receptor 2 (1-217) disease mutant P164S Deposited 2013-04-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–217（217 aa） Fragment：N-terminal domain (UNP residues 1-217)	Mutation：P164S	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；294 K；0.2 M sodium malonate, 5-15% saturated ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K	Resolution 2.41 Å R-free 0.273
4KEJ Crystal structure of mouse Ryanodine Receptor 2 (1-217) disease mutant R169Q Deposited 2013-04-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–217（217 aa） Fragment：N-terminal domain (UNP residues 1-217)	Mutation：R169Q	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；294 K；0.2 M sodium malonate, 5-15% saturated ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K	Resolution 2.55 Å R-free 0.297
4KEK Crystal structure of mouse Ryanodine Receptor 2 (1-217) disease mutant R176Q Deposited 2013-04-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–217（217 aa） Fragment：N-terminal domain (UNP residues 1-217)	Mutation：R176Q	SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；294 K；0.2 M sodium malonate, 5-15% saturated ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K	Resolution 2.15 Å R-free 0.290
4L4H Crystal structure of mouse Ryanodine Receptor isoform 2 (RyR2) 1-547 Deposited 2013-06-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–547（547 aa） Fragment：N-terminal domains (UNP residues 1-547)	Not recorded	CL CHLORIDE ION × 1 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.15；294 K；6% PEG3350, 0.1 M Tris, pH 8.15, 0.8% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K	Resolution 2.00 Å R-free 0.251
4L4I Crystal structure of mouse Ryanodine Receptor isoform 2 (RyR2) 1-547 disease mutant R420Q Deposited 2013-06-07	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–547（547 aa） Fragment：N-terminal domains (UNP residues 1-547)	Mutation：R420Q	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；294 K；0.1 M Tris, pH 8.15, 5-10% PEG3350, 1% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K	Resolution 2.15 Å R-free 0.263
4P9I Crystal Structure of mouse Ryanodine Receptor 2 SPRY2 Domain (1080-1253) Deposited 2014-04-04	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1080–1253（174 aa） Fragment：UNP residues 1080-1253	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium formate, peg 3350	Resolution 1.34 Å R-free 0.163
4P9I Crystal Structure of mouse Ryanodine Receptor 2 SPRY2 Domain (1080-1253) Deposited 2014-04-04	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1080–1253（174 aa） Fragment：UNP residues 1080-1253	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium formate, peg 3350	Resolution 1.34 Å R-free 0.163
4P9L Crystal Structure of mouse Ryanodine Receptor 2 SPRY2 Domain (1080-1253) disease mutant A1107M Deposited 2014-04-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1080–1253（174 aa） Fragment：UNP Residues 1080-1253	Mutation：A1107M	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium formate, peg 3350	Resolution 1.44 Å R-free 0.184
4P9L Crystal Structure of mouse Ryanodine Receptor 2 SPRY2 Domain (1080-1253) disease mutant A1107M Deposited 2014-04-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1080–1253（174 aa） Fragment：UNP Residues 1080-1253	Mutation：A1107M	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium formate, peg 3350	Resolution 1.44 Å R-free 0.184
5C33 Crystal Structure of Mouse Ryanodine Receptor 2 SPRY1 Domain Deposited 2015-06-16	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 650–844（195 aa） Fragment：SPRY1 domain (UNP residues 650-844)	Non-standard monomer：Yes (specific site not provided by mmCIF)	IPA ISOPROPYL ALCOHOL × 1 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；277 K；0.1 M sodium acetate, pH 5.0, 0.6-0.9 M sodium sulfate	Resolution 1.21 Å R-free 0.172
5C33 Crystal Structure of Mouse Ryanodine Receptor 2 SPRY1 Domain Deposited 2015-06-16	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 650–844（195 aa） Fragment：SPRY1 domain (UNP residues 650-844)	Non-standard monomer：Yes (specific site not provided by mmCIF)	IPA ISOPROPYL ALCOHOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；277 K；0.1 M sodium acetate, pH 5.0, 0.6-0.9 M sodium sulfate	Resolution 1.21 Å R-free 0.172
5VSN Crystal structure of mouse ryanodine receptor 2 SPRY2 domain (1080-1253) disease mutant P1124L Deposited 2017-05-12	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1084–1252（169 aa） Fragment：SPRY2 domain (UNP residues 1084-1252)	Mutation：P1124L	GOL GLYCEROL × 1 K POTASSIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.1 M potassium thiocyanate, 30% PEG2000 MME	Resolution 1.44 Å R-free 0.193
6J6L Crystal structure of mouse Ryanodine Receptor 2 SPRY1 Domain (650-844) disease mutant I784F Deposited 2019-01-15	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 650–844（195 aa） Chain B 650–844（195 aa）	Mutation：I784F Mutation：I784F	IPA ISOPROPYL ALCOHOL × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；0.1 M sodium acetate (PH4.5), 0.8 M sodium sulphate.	Resolution 1.45 Å R-free 0.204
6MM5 Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 peptide (2799-2810) Deposited 2018-09-29	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 2799–2810（12 aa） Fragment：residues 2799-2810	Not recorded	MG MAGNESIUM ION × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 EDO 1,2-ETHANEDIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；0.2M trimethylamine N-oxide, 0.1 M tris-HCl, 20% (w/v) PEG monomethyl ether 2K 25% (v/V) ethylene glycol	Resolution 1.95 Å R-free 0.227
6MM6 Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 phosphorylation domain (2699-2904) Deposited 2018-09-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain F 2699–2904（206 aa） Fragment：residues 2699-2904	Mutation：K2879A	CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.05M HEPES, 0.05 M KCl, 0.01M MgCl2, 15% (w/v) PEG 6K, and 25% (v/v) ethylene glycol	Resolution 2.39 Å R-free 0.261
6MM6 Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 phosphorylation domain (2699-2904) Deposited 2018-09-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain D 2699–2904（206 aa） Fragment：residues 2699-2904	Mutation：K2879A	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.05M HEPES, 0.05 M KCl, 0.01M MgCl2, 15% (w/v) PEG 6K, and 25% (v/v) ethylene glycol	Resolution 2.39 Å R-free 0.261
6MM7 Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 K2879A, S2813D phosphomimetic (2699-2904) crystal form 1 Deposited 2018-09-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain C 2699–2904（206 aa） Fragment：residues 2699-2904	Mutation：K2879A, S2813D	EDO 1,2-ETHANEDIOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.1 M magnesium formate and 25% (w/v) PEG 3350; 0.05 M HEPES, and 25% ethylene glycol	Resolution 1.85 Å R-free 0.238
6MM7 Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 K2879A, S2813D phosphomimetic (2699-2904) crystal form 1 Deposited 2018-09-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain E 2699–2904（206 aa） Fragment：residues 2699-2904	Mutation：K2879A, S2813D	EDO 1,2-ETHANEDIOL × 1 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.1 M magnesium formate and 25% (w/v) PEG 3350; 0.05 M HEPES, and 25% ethylene glycol	Resolution 1.85 Å R-free 0.238
6MM8 Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 K2879A, S2813D phosphomimetic (2699-2904) crystal form 2 Deposited 2018-09-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 2699–2904（206 aa） Fragment：residues 2699-2904	Mutation：K2879A, S2813D	PEG DI(HYDROXYETHYL)ETHER × 1 ACY ACETIC ACID × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 EDO 1,2-ETHANEDIOL × 5 CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.15 M HEPES pH 7.5, 15% (w/v) PEG 20k, and 25% (v/v) ethylene glycol	Resolution 1.85 Å R-free 0.221
6WOU Cryo-EM structure of recombinant mouse Ryanodine Receptor type 2 mutant R176Q in complex with FKBP12.6 in nanodisc Deposited 2020-04-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–4966（4966 aa） Chain B 1–4966（4966 aa） Chain C 1–4966（4966 aa） Chain D 1–4966（4966 aa）	Mutation：R176Q Mutation：R176Q Mutation：R176Q Mutation：R176Q	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.27 Å
6WOV Cryo-EM structure of recombinant mouse Ryanodine Receptor type 2 wild type in complex with FKBP12.6 Deposited 2020-04-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–4966（4966 aa） Chain B 1–4966（4966 aa） Chain C 1–4966（4966 aa） Chain D 1–4966（4966 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.10 Å
6Y4P Calmodulin N53I variant bound to cardiac ryanodine receptor (RyR2) calmodulin binding domain Deposited 2020-02-21	Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 3580–3606（27 aa）	Not recorded	CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1 M Sodium Acetate pH 4.70 and 23 % PEG 550 MME	Resolution 2.13 Å R-free 0.246
7VML Structure of recombinant RyR2 (EGTA dataset, class 1&2, closed state) Deposited 2021-10-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–1311（1311 aa） Chain A 1340–1386（47 aa） Chain A 1566–2507（942 aa） Chain A 2712–2915（204 aa） Chain A 3611–4966（1356 aa） Chain B 1–1311（1311 aa） Chain B 1340–1386（47 aa） Chain B 1566–2507（942 aa） Chain B 2712–2915（204 aa） Chain B 3611–4966（1356 aa） Chain C 1–1311（1311 aa） Chain C 1340–1386（47 aa） Chain C 1566–2507（942 aa） Chain C 2712–2915（204 aa） Chain C 3611–4966（1356 aa） Chain D 1–1311（1311 aa） Chain D 1340–1386（47 aa） Chain D 1566–2507（942 aa） Chain D 2712–2915（204 aa） Chain D 3611–4966（1356 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7VMM Structure of recombinant RyR2 (EGTA dataset, class 1, closed state) Deposited 2021-10-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–1311（1311 aa） Chain A 1340–1386（47 aa） Chain A 1566–2507（942 aa） Chain A 2712–2915（204 aa） Chain A 3611–4966（1356 aa） Chain B 1–1311（1311 aa） Chain B 1340–1386（47 aa） Chain B 1566–2507（942 aa） Chain B 2712–2915（204 aa） Chain B 3611–4966（1356 aa） Chain C 1–1311（1311 aa） Chain C 1340–1386（47 aa） Chain C 1566–2507（942 aa） Chain C 2712–2915（204 aa） Chain C 3611–4966（1356 aa） Chain D 1–1311（1311 aa） Chain D 1340–1386（47 aa） Chain D 1566–2507（942 aa） Chain D 2712–2915（204 aa） Chain D 3611–4966（1356 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7VMN Structure of recombinant RyR2 (EGTA dataset, class 2, closed state) Deposited 2021-10-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–1311（1311 aa） Chain A 1340–1386（47 aa） Chain A 1566–2507（942 aa） Chain A 2712–2915（204 aa） Chain A 3611–4966（1356 aa） Chain B 1–1311（1311 aa） Chain B 1340–1386（47 aa） Chain B 1566–2507（942 aa） Chain B 2712–2915（204 aa） Chain B 3611–4966（1356 aa） Chain C 1–1311（1311 aa） Chain C 1340–1386（47 aa） Chain C 1566–2507（942 aa） Chain C 2712–2915（204 aa） Chain C 3611–4966（1356 aa） Chain D 1–1311（1311 aa） Chain D 1340–1386（47 aa） Chain D 1566–2507（942 aa） Chain D 2712–2915（204 aa） Chain D 3611–4966（1356 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7VMO Structure of recombinant RyR2 (Ca2+ dataset, class 1, open state) Deposited 2021-10-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–1311（1311 aa） Chain A 1340–1386（47 aa） Chain A 1566–2507（942 aa） Chain A 2712–2915（204 aa） Chain A 3611–4966（1356 aa） Chain B 1–1311（1311 aa） Chain B 1340–1386（47 aa） Chain B 1566–2507（942 aa） Chain B 2712–2915（204 aa） Chain B 3611–4966（1356 aa） Chain C 1–1311（1311 aa） Chain C 1340–1386（47 aa） Chain C 1566–2507（942 aa） Chain C 2712–2915（204 aa） Chain C 3611–4966（1356 aa） Chain D 1–1311（1311 aa） Chain D 1340–1386（47 aa） Chain D 1566–2507（942 aa） Chain D 2712–2915（204 aa） Chain D 3611–4966（1356 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7VMP Structure of recombinant RyR2 (Ca2+ dataset, class 2, open state) Deposited 2021-10-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–1311（1311 aa） Chain A 1340–1386（47 aa） Chain A 1566–2507（942 aa） Chain A 2712–2915（204 aa） Chain A 3611–4966（1356 aa） Chain B 1–1311（1311 aa） Chain B 1340–1386（47 aa） Chain B 1566–2507（942 aa） Chain B 2712–2915（204 aa） Chain B 3611–4966（1356 aa） Chain C 1–1311（1311 aa） Chain C 1340–1386（47 aa） Chain C 1566–2507（942 aa） Chain C 2712–2915（204 aa） Chain C 3611–4966（1356 aa） Chain D 1–1311（1311 aa） Chain D 1340–1386（47 aa） Chain D 1566–2507（942 aa） Chain D 2712–2915（204 aa） Chain D 3611–4966（1356 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
7VMQ Structure of recombinant RyR2 (Ca2+ dataset, class 3, open state) Deposited 2021-10-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–1311（1311 aa） Chain A 1340–1386（47 aa） Chain A 1566–2507（942 aa） Chain A 2712–2915（204 aa） Chain A 3611–4966（1356 aa） Chain B 1–1311（1311 aa） Chain B 1340–1386（47 aa） Chain B 1566–2507（942 aa） Chain B 2712–2915（204 aa） Chain B 3611–4966（1356 aa） Chain C 1–1311（1311 aa） Chain C 1340–1386（47 aa） Chain C 1566–2507（942 aa） Chain C 2712–2915（204 aa） Chain C 3611–4966（1356 aa） Chain D 1–1311（1311 aa） Chain D 1340–1386（47 aa） Chain D 1566–2507（942 aa） Chain D 2712–2915（204 aa） Chain D 3611–4966（1356 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
7VMR Structure of recombinant RyR2 mutant K4593A (EGTA dataset) Deposited 2021-10-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–1311（1311 aa） Chain A 1340–1386（47 aa） Chain A 1566–2507（942 aa） Chain A 2712–2915（204 aa） Chain A 3611–4966（1356 aa） Chain B 1–1311（1311 aa） Chain B 1340–1386（47 aa） Chain B 1566–2507（942 aa） Chain B 2712–2915（204 aa） Chain B 3611–4966（1356 aa） Chain C 1–1311（1311 aa） Chain C 1340–1386（47 aa） Chain C 1566–2507（942 aa） Chain C 2712–2915（204 aa） Chain C 3611–4966（1356 aa） Chain D 1–1311（1311 aa） Chain D 1340–1386（47 aa） Chain D 1566–2507（942 aa） Chain D 2712–2915（204 aa） Chain D 3611–4966（1356 aa）	Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593 Mutation：K4593	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
7VMS Structure of recombinant RyR2 mutant K4593A (Ca2+ dataset) Deposited 2021-10-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–1311（1311 aa） Chain A 1340–1386（47 aa） Chain A 1566–2507（942 aa） Chain A 2712–2915（204 aa） Chain A 3611–4966（1356 aa） Chain B 1–1311（1311 aa） Chain B 1340–1386（47 aa） Chain B 1566–2507（942 aa） Chain B 2712–2915（204 aa） Chain B 3611–4966（1356 aa） Chain C 1–1311（1311 aa） Chain C 1340–1386（47 aa） Chain C 1566–2507（942 aa） Chain C 2712–2915（204 aa） Chain C 3611–4966（1356 aa） Chain D 1–1311（1311 aa） Chain D 1340–1386（47 aa） Chain D 1566–2507（942 aa） Chain D 2712–2915（204 aa） Chain D 3611–4966（1356 aa）	Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A Mutation：K4593A	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8DTY Recombinant mouse RyR2 triple phosphomimetic mutant S2807D/S2813D/S2030D in complex with FKBP12.6 and nanodisc under closed-state conditions Deposited 2022-07-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–4966（4966 aa） Chain B 1–4966（4966 aa） Chain C 1–4966（4966 aa） Chain D 1–4966（4966 aa）	Mutation：S2807D, S2813D, S2030D Mutation：S2807D, S2813D, S2030D Mutation：S2807D, S2813D, S2030D Mutation：S2807D, S2813D, S2030D	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
8DTZ Recombinant mouse RyR2 triple phosphonull mutant S2807A/S2813A/S2030A in complex with FKBP12.6 and nanodisc under closed-state conditions Deposited 2022-07-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–4966（4966 aa） Chain B 1–4966（4966 aa） Chain C 1–4966（4966 aa） Chain D 1–4966（4966 aa）	Mutation：S2807A, S2813A, S2030A Mutation：S2807A, S2813A, S2030A Mutation：S2807A, S2813A, S2030A Mutation：S2807A, S2813A, S2030A	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8DVV Recombinant mouse RyR2 triple phosphomimetic mutant S2807D/S2813D/S2030D in complex with FKBP12.6 and nanodisc under open-state conditions Deposited 2022-07-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–4966（4966 aa） Chain B 1–4966（4966 aa） Chain C 1–4966（4966 aa） Chain D 1–4966（4966 aa）	Mutation：S2807D, S2813D, S2030D Mutation：S2807D, S2813D, S2030D Mutation：S2807D, S2813D, S2030D Mutation：S2807D, S2813D, S2030D	CA CALCIUM ION × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.68 Å
8RRS Structure of mouse RyR2 solubilised in detergent in open state in complex with Ca2+, ATP, caffeine and Nb9657. Deposited 2024-01-23	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–4966（4966 aa） Chain C 1–4966（4966 aa） Chain E 1–4966（4966 aa） Chain F 1–4966（4966 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å

31 other PDB entries and 38 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RYR2_MOUSE
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 4–30； UniProt 3580–3606

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	6y4o
Deposition date deposition_date	2020-02-21
Structure title title	Calmodulin bound to cardiac ryanodine receptor (RyR2) calmodulin binding domain
Keywords keywords	ion channel, calcium, calmodulin, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	18.14 Å
Radius of gyration Rg (electron density) rg_electron	16.98 Å
Forward intensity I(0) i0	6034370.00
Molecular weight molecular_weight	17314.0 kDa
Excluded volume excluded_volume	21385 Å³
Envelope volume envelope_volume	24899 Å³
Hydration-shell volume shell_volume	13083 Å³
Envelope diameter envelope_diameter	57.7 Å
Shell Rg shell_rg	21.70 Å
Envelope Rg envelope_rg	16.94 Å
Shape Rg shape_rg	16.98 Å
Total Rg total_rg	17.78 Å
Total atoms total_atoms	2311
Residues n_residues	162
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	58.8 Å
Rg (real space) rg_real	18.16 Å
Rg uncertainty (real space) rg_real_error	0.30 Å
I(0) (real space) i0_real	6.0340e+06
I(0) uncertainty (real space) i0_real_error	7.8230e+04
Rg (reciprocal space) rg_reciprocal	18.16 Å
I(0) (reciprocal space) i0_reciprocal	6034000.0000
Solution quality estimate total_estimate	0.8873
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	18.3 Å
Skewness Skewness skewness	0.316
Kurtosis Kurtosis kurtosis	-0.487
Angular range angular_range	— – 0.4400 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	1107000.0000
Real-space data points n_real_points	75
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.853; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.987; Smooth: 0.984

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Description description	Calmodulin-2
Molecular weight formula_weight	16852.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	2368

MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	Human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	Ryanodine receptor 2
Molecular weight formula_weight	3478.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

SNARSKKAVWHKLLSKQRKRAVVACFRMAP

来源 Source

来源生物 organism	Mus musculus
通用名 common_name	Mouse
NCBI Class编号 ncbi_taxonomy_id	10090
宿主生物 host_organism	Escherichia coli

Entity 3 Entity 3 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 4 Entity 4 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	49

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_id	d6y4oa_
Class class	a — All alpha proteins
Fold Fold fold	a.39 — EF Hand-like
Superfamily Superfamily superfamily	a.39.1 — EF-hand
Family Family family	a.39.1.5 — Calmodulin-like

CATH v4.4 (1 domains)

Domain ID domain_id	6y4oA01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	238 — Recoverin; domain 1
Homologous superfamily homologous superfamily	10 — EF-hand

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8. Citations (1)

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Unit-cell length a length_a	38.037 Å
Unit-cell length b length_b	43.557 Å
Unit-cell length c length_c	90.102 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	4

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	orthorhombic

Resolution resolution	1.835 Å
Low-resolution limit resolution_low	27.303 Å
R factor r_factor	0.1797
R_work r_work	0.1765
R_free r_free	0.2076

fit	/app/data/saxs_computation/y4/6y4o/6y4o.fit
pddf	/app/data/pr_computation/y4/6y4o/6y4o.pr.out
profile	/app/data/saxs_computation/y4/6y4o/6y4o.dat

Calmodulin bound to cardiac ryanodine receptor (RyR2) calmodulin binding domain

1. Protein Identity and Related Structures Protein Identity & Related Structures

Calmodulin-2

State in the Current Structure

Other States of the Same Protein in the Database

Ryanodine receptor 2

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 polymer

Polymer Polymer

来源 Source

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 water

7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

CATH v4.4 (1 domains)

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)