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6zuz

TFIIS N-terminal domain (TND) from human Elongin-A

Method: SOLUTION NMR Dmax: 40.4 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Elongin-A

Homo sapiens

UniProt Q14241

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 26–108 Not recorded No other associated polymer SOLUTION NMR NMR measurement conditions：pH 7.4；298 K；Ionic strength (raw mmCIF value) 225；Pressure 1 NMR sample composition：0.5 mM [U-13C; U-15N] ELOA, 25 mM deuterated TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O | 95% H2O/5% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6ZUZ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4HFX Crystal structure of a transcription elongation factor B polypeptide 3 from Homo sapiens, Northeast Structural Genomics consortium target id HR4748B. Deposited 2012-10-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 597–682（86 aa） Fragment：F-box domain residues 597-682
Chain D 597–682（86 aa） Fragment：F-box domain residues 597-682
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions Microbatch under oil method；pH 9；277 K；1.88M Na2s2o3, 0.1M TAPS PH9, Microbatch under oil method, temperature 277K
Resolution 2.54 Å R-free 0.263
4HFX Crystal structure of a transcription elongation factor B polypeptide 3 from Homo sapiens, Northeast Structural Genomics consortium target id HR4748B. Deposited 2012-10-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 597–682（86 aa） Fragment：F-box domain residues 597-682
Chain C 597–682（86 aa） Fragment：F-box domain residues 597-682
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Microbatch under oil method；pH 9；277 K；1.88M Na2s2o3, 0.1M TAPS PH9, Microbatch under oil method, temperature 277K
Resolution 2.54 Å R-free 0.263
8OEV Structure of the mammalian Pol II-SPT6-Elongin complex, lacking ELOA latch (composite structure, structure 3) Deposited 2023-03-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 16 PDB declaration: nonadecameric
Chain M 1–798（798 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
8OEW Structure of the mammalian Pol II-Elongin complex, lacking the ELOA latch (composite structure, structure 2) Deposited 2023-03-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 15 PDB declaration: octadecameric
Chain M 1–798（798 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8OF0 Structure of the mammalian Pol II-SPT6-Elongin complex, Structure 1 Deposited 2023-03-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 16 PDB declaration: nonadecameric
Chain M 1–798（798 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.05 Å

4 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ELOA1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 6–88； UniProt 26–108

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6zuz

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6zuz
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6zuz
Deposition date deposition_date2020-07-23
Structure title titleTFIIS N-terminal domain (TND) from human Elongin-A
Keywords keywordselongation factor, TRANSCRIPTION; TRANSCRIPTION
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier15.07 Å
Radius of gyration Rg (electron density) rg_electron14.32 Å
Forward intensity I(0) i01946080000.00
Molecular weight molecular_weight387810.0 kDa
Excluded volume excluded_volume492820 ų
Envelope volume envelope_volume49619 ų
Hydration-shell volume shell_volume19657 ų
Envelope diameter envelope_diameter78.0 Å
Shell Rg shell_rg28.00 Å
Envelope Rg envelope_rg22.53 Å
Shape Rg shape_rg14.27 Å
Total Rg total_rg14.75 Å
Total atoms total_atoms56400
Residues n_residues3600
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax40.4 Å
Rg (real space) rg_real14.22 Å
Rg uncertainty (real space) rg_real_error0.06 Å
I(0) (real space) i0_real1.8530e+09
I(0) uncertainty (real space) i0_real_error1.8090e+07
Rg (reciprocal space) rg_reciprocal15.23 Å
I(0) (reciprocal space) i0_reciprocal1946000000.0000
Solution quality estimate total_estimate0.6652
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary15.3 Å
Skewness Skewness skewness0.313
Kurtosis Kurtosis kurtosis-0.350
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha5.0900
Highest regularization parameter α highest_alpha185300.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.007; Oscil: 0.907; Stabil: 0.976; Sysdev: 0.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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8. Citations (1)

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9. Files and Curves (10)