7afa

Bacterial 30S ribosomal subunit assembly complex state F (head domain)

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

30S ribosomal protein S2

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 8 RNA 1	16SrRNA (head domain of the 30S ribosome) × 1 30S ribosomal protein S3 × 1 （C3SQX2） 30S ribosomal protein S7 × 1 （A0A5Q2GFB5） 30S ribosomal protein S9 × 1 （C3SRY2） 30S ribosomal protein S10 × 1 （C3SQT7） 30S ribosomal protein S13 × 1 （C3SR52） 30S ribosomal protein S14 × 1 （C3SR07） 30S ribosomal protein S19 × 1 （C3SQW2） MAGNESIUM ION × 25 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	C3TPN2_ECOLX
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–241； UniProt 1–241

30S ribosomal protein S3

OrganismNot specified

UniProt C3SQX2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 8 RNA 1	16SrRNA (head domain of the 30S ribosome) × 1 30S ribosomal protein S2 × 1 （C3TPN2） 30S ribosomal protein S7 × 1 （A0A5Q2GFB5） 30S ribosomal protein S9 × 1 （C3SRY2） 30S ribosomal protein S10 × 1 （C3SQT7） 30S ribosomal protein S13 × 1 （C3SR52） 30S ribosomal protein S14 × 1 （C3SR07） 30S ribosomal protein S19 × 1 （C3SQW2） MAGNESIUM ION × 25 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	C3SQX2_ECOLX
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–233； UniProt 1–233

30S ribosomal protein S7

OrganismNot specified

UniProt A0A5Q2GFB5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 8 RNA 1	16SrRNA (head domain of the 30S ribosome) × 1 30S ribosomal protein S2 × 1 （C3TPN2） 30S ribosomal protein S3 × 1 （C3SQX2） 30S ribosomal protein S9 × 1 （C3SRY2） 30S ribosomal protein S10 × 1 （C3SQT7） 30S ribosomal protein S13 × 1 （C3SR52） 30S ribosomal protein S14 × 1 （C3SR07） 30S ribosomal protein S19 × 1 （C3SQW2） MAGNESIUM ION × 25 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A5Q2GFB5_ECOLX
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain G； PDBConstruct 1–179； UniProt 1–179

30S ribosomal protein S9

OrganismNot specified

UniProt C3SRY2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 8 RNA 1	16SrRNA (head domain of the 30S ribosome) × 1 30S ribosomal protein S2 × 1 （C3TPN2） 30S ribosomal protein S3 × 1 （C3SQX2） 30S ribosomal protein S7 × 1 （A0A5Q2GFB5） 30S ribosomal protein S10 × 1 （C3SQT7） 30S ribosomal protein S13 × 1 （C3SR52） 30S ribosomal protein S14 × 1 （C3SR07） 30S ribosomal protein S19 × 1 （C3SQW2） MAGNESIUM ION × 25 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	C3SRY2_ECOLX
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain I； PDBConstruct 1–130； UniProt 1–130

30S ribosomal protein S10

OrganismNot specified

UniProt C3SQT7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 8 RNA 1	16SrRNA (head domain of the 30S ribosome) × 1 30S ribosomal protein S2 × 1 （C3TPN2） 30S ribosomal protein S3 × 1 （C3SQX2） 30S ribosomal protein S7 × 1 （A0A5Q2GFB5） 30S ribosomal protein S9 × 1 （C3SRY2） 30S ribosomal protein S13 × 1 （C3SR52） 30S ribosomal protein S14 × 1 （C3SR07） 30S ribosomal protein S19 × 1 （C3SQW2） MAGNESIUM ION × 25 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	C3SQT7_ECOLX
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain J； PDBConstruct 1–103； UniProt 1–103

30S ribosomal protein S13

OrganismNot specified

UniProt C3SR52

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 8 RNA 1	16SrRNA (head domain of the 30S ribosome) × 1 30S ribosomal protein S2 × 1 （C3TPN2） 30S ribosomal protein S3 × 1 （C3SQX2） 30S ribosomal protein S7 × 1 （A0A5Q2GFB5） 30S ribosomal protein S9 × 1 （C3SRY2） 30S ribosomal protein S10 × 1 （C3SQT7） 30S ribosomal protein S14 × 1 （C3SR07） 30S ribosomal protein S19 × 1 （C3SQW2） MAGNESIUM ION × 25 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	C3SR52_ECOLX
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain M； PDBConstruct 1–118； UniProt 1–118

30S ribosomal protein S14

OrganismNot specified

UniProt C3SR07

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 8 RNA 1	16SrRNA (head domain of the 30S ribosome) × 1 30S ribosomal protein S2 × 1 （C3TPN2） 30S ribosomal protein S3 × 1 （C3SQX2） 30S ribosomal protein S7 × 1 （A0A5Q2GFB5） 30S ribosomal protein S9 × 1 （C3SRY2） 30S ribosomal protein S10 × 1 （C3SQT7） 30S ribosomal protein S13 × 1 （C3SR52） 30S ribosomal protein S19 × 1 （C3SQW2） MAGNESIUM ION × 25 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	C3SR07_ECOLX
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain N； PDBConstruct 1–101； UniProt 1–101

30S ribosomal protein S19

OrganismNot specified

UniProt C3SQW2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 8 RNA 1	16SrRNA (head domain of the 30S ribosome) × 1 30S ribosomal protein S2 × 1 （C3TPN2） 30S ribosomal protein S3 × 1 （C3SQX2） 30S ribosomal protein S7 × 1 （A0A5Q2GFB5） 30S ribosomal protein S9 × 1 （C3SRY2） 30S ribosomal protein S10 × 1 （C3SQT7） 30S ribosomal protein S13 × 1 （C3SR52） 30S ribosomal protein S14 × 1 （C3SR07） MAGNESIUM ION × 25 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	C3SQW2_ECOLX
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain S； PDBConstruct 1–92； UniProt 1–92

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7afa
Deposition date deposition_date	2020-09-19
Structure title title	Bacterial 30S ribosomal subunit assembly complex state F (head domain)
Keywords keywords	Cryo-EM, 30S biogenesis, ribosome assembly, RbfA, RsgA, YjeQ, RimP, KsgA, RsmA, RIBOSOME; RIBOSOME
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7afa__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7afa__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

7afa__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	41.43 Å
Rg (electron density)	42.58 Å
Total Rg	42.67 Å
Atom count	18482
Residues	1554
Excluded volume	293810 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`7afa__assembly_1__model_1`	nonameric （9）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (11)

Entity 1 Entity 1 polymer

Description description	16SrRNA (head domain of the 30S ribosome)
Molecular weight formula_weight	499528.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	1
Atom count atom_count	18482

AAAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGAAGCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAUGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAAACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUGGGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGAACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCCGCGUGUAUGAAGAAGGCCUUCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUUGACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAGGGUGCAAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAGAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAUACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACCGGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCCUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCGCCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAGAAUGUGCCUUCGGGAACCGUGAGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAAAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCUUUGUUGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUCAUCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCGACCUCGCGAGAGCAAGCGGACCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAGAAUGCCACGGUGAAUACGUUCCCGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGUAGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAACAAGGUAACCGUAGGGGAACCUGCGGUUGGAUCACCUCCUUA

Entity 2 Entity 2 polymer

Description description	30S ribosomal protein S2
Molecular weight formula_weight	26781.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDALFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQDLASQAEESFVEAE

Entity 3 Entity 3 polymer

Description description	30S ribosomal protein S3
Molecular weight formula_weight	26031.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGAEIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPEKPAAQPKKQQRKGRK

Entity 4 Entity 4 polymer

Description description	30S ribosomal protein S7
Molecular weight formula_weight	20055.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLRSFSHQAGASSKQPALGYLN

Entity 5 Entity 5 polymer

Description description	30S ribosomal protein S9
Molecular weight formula_weight	14886.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETARMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAGFVTRDARQVERKKVGLRKARRRPQFSKR

Entity 6 Entity 6 polymer

Description description	30S ribosomal protein S10
Molecular weight formula_weight	11755.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISLG

Entity 7 Entity 7 polymer

Description description	30S ribosomal protein S13
Molecular weight formula_weight	13128.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M

MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVKISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKPIKK

Entity 8 Entity 8 polymer

Description description	30S ribosomal protein S14
Molecular weight formula_weight	11606.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNAVLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW

Entity 9 Entity 9 polymer

Description description	30S ribosomal protein S19
Molecular weight formula_weight	10455.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S

MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNMIGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK

Entity 10 Entity 10 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	25

Entity 11 Entity 11 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	1

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Resolution resolution	2.95 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	21537

Bacterial 30S ribosomal subunit assembly complex state F (head domain)

1. Protein Identity and Related Structures Protein Identity & Related Structures

30S ribosomal protein S2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

30S ribosomal protein S3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

30S ribosomal protein S7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

30S ribosomal protein S9

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

30S ribosomal protein S10

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

30S ribosomal protein S13

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

30S ribosomal protein S14

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

30S ribosomal protein S19

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

7afa__assembly_1__model_1

7afa__assembly_1__model_1 | I(q)

7afa__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (11)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer