7afo

Bacterial 30S ribosomal subunit assembly complex state B (body domain)

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

30S ribosomal protein S4

OrganismNot specified

UniProt C3SR62

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SR62_ECOLX
Isoform —
PDB entities 2
Chains and sequence ranges Author chain D; PDBConstruct 1–206; UniProt 1–206

30S ribosomal protein S5

OrganismNot specified

UniProt C3SR27

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SR27_ECOLX
Isoform —
PDB entities 3
Chains and sequence ranges Author chain E; PDBConstruct 1–167; UniProt 1–167

30S ribosomal protein S6

OrganismNot specified

UniProt P02358

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RS6_ECOLI
Isoform —
PDB entities 4
Chains and sequence ranges Author chain F; PDBConstruct 1–135; UniProt 1–135

30S ribosomal protein S8

OrganismNot specified

UniProt C3SR12

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SR12_ECOLX
Isoform —
PDB entities 5
Chains and sequence ranges Author chain H; PDBConstruct 1–130; UniProt 1–130

30S ribosomal protein S11

OrganismNot specified

UniProt C3SR57

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SR57_ECOLX
Isoform —
PDB entities 6
Chains and sequence ranges Author chain K; PDBConstruct 1–129; UniProt 1–129

30S ribosomal protein S12

OrganismNot specified

UniProt C3SQR7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SQR7_ECOLX
Isoform —
PDB entities 7
Chains and sequence ranges Author chain L; PDBConstruct 1–124; UniProt 1–124

30S ribosomal protein S15

OrganismNot specified

UniProt C3SSQ7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SSQ7_ECOLX
Isoform —
PDB entities 8
Chains and sequence ranges Author chain O; PDBConstruct 1–89; UniProt 1–89

30S ribosomal protein S16

OrganismNot specified

UniProt C3SYP2

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SYP2_ECOLX
Isoform —
PDB entities 9
Chains and sequence ranges Author chain P; PDBConstruct 1–82; UniProt 1–82

30S ribosomal protein S17

OrganismNot specified

UniProt C3SQY7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SQY7_ECOLX
Isoform —
PDB entities 10
Chains and sequence ranges Author chain Q; PDBConstruct 1–84; UniProt 1–84

30S ribosomal protein S18

OrganismNot specified

UniProt C3SFP7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SFP7_ECOLX
Isoform —
PDB entities 11
Chains and sequence ranges Author chain R; PDBConstruct 1–75; UniProt 1–75

30S ribosomal protein S20

OrganismNot specified

UniProt C3TRH7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3TRH7_ECOLX
Isoform —
PDB entities 12
Chains and sequence ranges Author chain T; PDBConstruct 1–87; UniProt 1–87

30S ribosomal protein S21

OrganismNot specified

UniProt C3STZ7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3STZ7_ECOLX
Isoform —
PDB entities 13
Chains and sequence ranges Author chain U; PDBConstruct 1–71; UniProt 1–71

Ribosome maturation factor RimP

Escherichia coli

UniProt A0A0J3VRH1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosomal RNA small subunit methyltransferase A × 1 (I4T5U1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name A0A0J3VRH1_ECOLX
Isoform —
PDB entities 14
Chains and sequence ranges Author chain X; PDBConstruct 1–150; UniProt 1–150

Ribosomal RNA small subunit methyltransferase A

Escherichia coli

UniProt I4T5U1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16SrRNA (body domain of the 30S ribosome) × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (C3SR27) 30S ribosomal protein S6 × 1 (P02358) 30S ribosomal protein S8 × 1 (C3SR12) 30S ribosomal protein S11 × 1 (C3SR57) 30S ribosomal protein S12 × 1 (C3SQR7) 30S ribosomal protein S15 × 1 (C3SSQ7) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (C3SQY7) 30S ribosomal protein S18 × 1 (C3SFP7) 30S ribosomal protein S20 × 1 (C3TRH7) 30S ribosomal protein S21 × 1 (C3STZ7) Ribosome maturation factor RimP × 1 (A0A0J3VRH1) MAGNESIUM ION × 20 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name I4T5U1_ECOLX
Isoform —
PDB entities 15
Chains and sequence ranges Author chain Y; PDBConstruct 1–273; UniProt 1–273

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id7afo
Deposition date deposition_date2020-09-19
Structure title titleBacterial 30S ribosomal subunit assembly complex state B (body domain)
Keywords keywordsCryo-EM, 30S biogenesis, ribosome assembly, RbfA, RsgA, YjeQ, RimP, KsgA, RsmA, RIBOSOME; RIBOSOME
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7afo__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7afo__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

7afo__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)56.05 Å
Rg (electron density)56.49 Å
Total Rg56.54 Å
Atom count34134
Residues2658
Excluded volume530970 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 7afo__assembly_1__model_1 pentadecameric (15) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (16)

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6. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

Domain ID domain_id7afoD01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1050 — Ribosomal Protein S4 Delta 41; Chain A, domain 1
Homologous superfamily homologous superfamily10 — Ribosomal protein S4/S9, N-terminal domain
Domain ID domain_id7afoD02
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology290 — Structural Genomics Hypothetical 15.5 Kd Protein In mrcA-pckA Intergenic Region; Chain A
Homologous superfamily homologous superfamily10 — RNA-binding S4 domain
Domain ID domain_id7afoX01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology300 — GMP Synthetase; Chain A, domain 3
Homologous superfamily homologous superfamily70 — RimP-like superfamily, N-terminal
Domain ID domain_id7afoX02
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily180 — Ribosome maturation factor RimP, C-terminal domain
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7. Citations (1)