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7aud

Structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Bloom syndrome protein,Bloom syndrome protein

Homo sapiens

UniProt P54132

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Monomer Protein 1 DNA 1 ;DNA (5'-D(*GP*TP*AP*CP*CP*CP*GP*AP*TP*GP*TP*GP*T)-3') ; × 1 ZINC ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1 N-(2,3-dimethyl-5-sulfamoylphenyl)-4-(2-methylthiazol-4-yl)benzamide × 1 MAGNESIUM ION × 1 water × 1 Consistent with all polymers
2 Protein–DNA Monomer Protein 1 DNA 1 ;DNA (5'-D(*GP*TP*AP*CP*CP*CP*GP*AP*TP*GP*TP*GP*T)-3') ; × 1 ZINC ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1 N-(2,3-dimethyl-5-sulfamoylphenyl)-4-(2-methylthiazol-4-yl)benzamide × 1 TETRAETHYLENE GLYCOL × 2 MAGNESIUM ION × 1 water × 2 Consistent with all polymers
3 Protein–DNA Monomer Protein 1 DNA 1 ;DNA (5'-D(*GP*TP*AP*CP*CP*CP*GP*AP*TP*GP*TP*GP*T)-3') ; × 1 ZINC ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1 N-(2,3-dimethyl-5-sulfamoylphenyl)-4-(2-methylthiazol-4-yl)benzamide × 1 TETRAETHYLENE GLYCOL × 1 MAGNESIUM ION × 1 water × 1 Consistent with all polymers
4 Protein–DNA Monomer Protein 1 DNA 1 ;DNA (5'-D(*GP*TP*AP*CP*CP*CP*GP*AP*TP*GP*TP*GP*T)-3') ; × 1 ZINC ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1 N-(2,3-dimethyl-5-sulfamoylphenyl)-4-(2-methylthiazol-4-yl)benzamide × 1 TETRAETHYLENE GLYCOL × 2 water × 1 Consistent with all polymers
5 Protein–DNA Monomer Protein 1 DNA 1 ;DNA (5'-D(*GP*TP*AP*CP*CP*CP*GP*AP*TP*GP*TP*GP*T)-3') ; × 1 ZINC ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1 N-(2,3-dimethyl-5-sulfamoylphenyl)-4-(2-methylthiazol-4-yl)benzamide × 1 TETRAETHYLENE GLYCOL × 1 MAGNESIUM ION × 1 water × 1 Consistent with all polymers
6 Protein–DNA Monomer Protein 1 DNA 1 ;DNA (5'-D(*GP*TP*AP*CP*CP*CP*GP*AP*TP*GP*TP*GP*T)-3') ; × 1 ZINC ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1 N-(2,3-dimethyl-5-sulfamoylphenyl)-4-(2-methylthiazol-4-yl)benzamide × 1 TETRAETHYLENE GLYCOL × 1 MAGNESIUM ION × 2 water × 2 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
2kv2 2mh9 2rrd 3we2 3we2 3we3 3we3 7auc

8 assemblies

Homodimer
5mk5 5mk5

2 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
4cdg 4cdg 4cgz 4o3m 7aud 7aud 7aud 7aud 7aud 7aud 7xuw 7xv0

12 assemblies

View Construct and Data Evidence
UniProt name BLM_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 27–461； UniProt 636–1070 Author chain A； PDBConstruct 467–563； UniProt 1202–1298 Author chain B； PDBConstruct 27–461； UniProt 636–1070 Author chain B； PDBConstruct 467–563； UniProt 1202–1298 Author chain C； PDBConstruct 27–461； UniProt 636–1070 Author chain C； PDBConstruct 467–563； UniProt 1202–1298 Author chain D； PDBConstruct 27–461； UniProt 636–1070 Author chain D； PDBConstruct 467–563； UniProt 1202–1298 Author chain E； PDBConstruct 27–461； UniProt 636–1070 Author chain E； PDBConstruct 467–563； UniProt 1202–1298 Author chain F； PDBConstruct 27–461； UniProt 636–1070 Author chain F； PDBConstruct 467–563； UniProt 1202–1298

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id7aud
Deposition date deposition_date2020-11-02
Structure title titleStructure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)
Keywords keywordsHelicase, RecQ, BLM, DNA Repair, Inhibitor, Allosteric, NUCLEAR PROTEIN; NUCLEAR PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7aud__assembly_5__model_1

Assembly 5 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7aud__assembly_5__model_1 | I(q)

10-2 10-1 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

7aud__assembly_5__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）29.09 Å
Rg (electron density)29.68 Å
Total Rg29.80 Å
Atom count4126
Residues523
Excluded volume72089 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 7aud__assembly_1__model_1 protein:DNA complex （2） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 7aud__assembly_2__model_1 protein:DNA complex （2） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 7aud__assembly_3__model_1 protein:DNA complex （2） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 7aud__assembly_4__model_1 protein:DNA complex （2） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 7aud__assembly_5__model_1 protein:DNA complex （2） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 7aud__assembly_6__model_1 protein:DNA complex （2） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (8)

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7. Citations (2)