SAXSdb — 7elh

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Minor core protein mu2

OrganismNot specified

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 17 RNA 9 PDB declaration: 26-meric（26） Consistent with all polymer counts	Chain A； UniProt 1–736	Not recorded	RNA-directed RNA polymerase × 1 （A0A0B5CSU4） transcript RNA × 1 Lambda 1 × 10 （F1ARN3） Lambda 1 × 5 （F1ARN3） RNA (60-MER) × 8 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 7ELH	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
9CYY Cryo-EM structure of MRV virion Deposited 2024-08-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric	Chain V 1–736（736 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Phosphate-buffered saline cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	Q6EDZ8_9REOV
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–736； UniProt 1–736

RNA-directed RNA polymerase

OrganismNot specified

UniProt A0A0B5CSU4

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 17 RNA 9 PDB declaration: 26-meric（26） Consistent with all polymer counts	Chain B； UniProt 1–1267	Not recorded	Minor core protein mu2 × 1 （Q6EDZ8） transcript RNA × 1 Lambda 1 × 10 （F1ARN3） Lambda 1 × 5 （F1ARN3） RNA (60-MER) × 8 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 7ELH	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
9CYY Cryo-EM structure of MRV virion Deposited 2024-08-03	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric	Chain W 1–1267（1267 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Phosphate-buffered saline cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	A0A0B5CSU4_9REOV
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–1267； UniProt 1–1267

Lambda 1

OrganismNot specified

UniProt F1ARN3

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein–RNA Heteromer Protein × 17 RNA 9 PDB declaration: 26-meric（26） Consistent with all polymer counts	Chain D； UniProt 181–1275 Chain E； UniProt 181–1275 Chain F； UniProt 181–1275 Chain G； UniProt 181–1275 Chain H； UniProt 181–1275 Chain I； UniProt 181–1275 Chain J； UniProt 181–1275 Chain K； UniProt 181–1275 Chain L； UniProt 181–1275 Chain M； UniProt 181–1275 Chain e； UniProt 1–180 Chain g； UniProt 1–180 Chain i； UniProt 1–180 Chain k； UniProt 1–180 Chain m； UniProt 1–180	Not recorded	Minor core protein mu2 × 1 （Q6EDZ8） RNA-directed RNA polymerase × 1 （A0A0B5CSU4） transcript RNA × 1 RNA (60-MER) × 8 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 7ELH	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
9CYX Cryo-EM structure of MRV full core Deposited 2024-08-02	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric	Chain B 1–1275（1275 aa） Chain H 1–1275（1275 aa） Chain I 1–1275（1275 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Phosphate-buffered saline cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9CYY Cryo-EM structure of MRV virion Deposited 2024-08-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric	Chain K 1–1275（1275 aa） Chain M 1–1275（1275 aa） Chain Y 1–1275（1275 aa） Chain Z 1–1275（1275 aa） Chain a 1–1275（1275 aa） Chain b 1–1275（1275 aa） Chain c 1–1275（1275 aa） Chain d 1–1275（1275 aa） Chain e 1–1275（1275 aa） Chain f 1–1275（1275 aa） Chain g 1–1275（1275 aa） Chain h 1–1275（1275 aa） Chain i 1–1275（1275 aa） Chain k 1–1275（1275 aa） Chain m 1–1275（1275 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；Phosphate-buffered saline cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	F1ARN3_9REOV
Isoform	—
PDB entities	4, 5
Chains and sequence ranges	Author chain D； PDBConstruct 1–1095； UniProt 181–1275 Author chain E； PDBConstruct 1–1095； UniProt 181–1275 Author chain F； PDBConstruct 1–1095； UniProt 181–1275 Author chain G； PDBConstruct 1–1095； UniProt 181–1275 Author chain H； PDBConstruct 1–1095； UniProt 181–1275 Author chain I； PDBConstruct 1–1095； UniProt 181–1275 Author chain J； PDBConstruct 1–1095； UniProt 181–1275 Author chain K； PDBConstruct 1–1095； UniProt 181–1275 Author chain L； PDBConstruct 1–1095； UniProt 181–1275 Author chain M； PDBConstruct 1–1095； UniProt 181–1275 Author chain e； PDBConstruct 1–180； UniProt 1–180 Author chain g； PDBConstruct 1–180； UniProt 1–180 Author chain i； PDBConstruct 1–180； UniProt 1–180 Author chain k； PDBConstruct 1–180； UniProt 1–180 Author chain m； PDBConstruct 1–180； UniProt 1–180

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7elh
Deposition date deposition_date	2021-04-11
Structure title title	In situ structure of transcriptional enzyme complex and capsid shell protein of mammalian reovirus at initiation state
Keywords keywords	asymmetric, mu2, lambda3, lambda1, VIRUS, VIRAL PROTEIN-TRANSFERASE-RNA complex; VIRAL PROTEIN/TRANSFERASE/RNA
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	97.76 Å
Radius of gyration Rg (electron density) rg_electron	98.73 Å
Forward intensity I(0) i0	37670700000.00
Molecular weight molecular_weight	1596600.0 kDa
Excluded volume excluded_volume	1971400 Å³
Envelope volume envelope_volume	3145400 Å³
Hydration-shell volume shell_volume	269160 Å³
Envelope diameter envelope_diameter	327.9 Å
Shell Rg shell_rg	88.15 Å
Envelope Rg envelope_rg	97.09 Å
Shape Rg shape_rg	98.84 Å
Total Rg total_rg	98.27 Å
Total atoms total_atoms	114995
Residues n_residues	13688
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	261.8 Å
Rg (real space) rg_real	94.10 Å
Rg uncertainty (real space) rg_real_error	0.81 Å
I(0) (real space) i0_real	3.6030e+10
I(0) uncertainty (real space) i0_real_error	6.7120e+08
Rg (reciprocal space) rg_reciprocal	96.23 Å
I(0) (reciprocal space) i0_reciprocal	37470000000.0000
Solution quality estimate total_estimate	0.9134
Solution quality rating solution_quality	EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	114.6 Å
Skewness Skewness skewness	0.212
Kurtosis Kurtosis kurtosis	-0.671
Angular range angular_range	— – 0.0800 Å⁻¹
Current regularization parameter α current_alpha	1.0600
Highest regularization parameter α highest_alpha	1550000000.0000
Real-space data points n_real_points	17
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.001; Oscil: 0.999; Stabil: 0.962; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.000

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	Minor core protein mu2
Molecular weight formula_weight	83331.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	114995

MAYIAVPAVVDSRSSEAIGLLESFGVDAGADANDVSYQDHDYVLDQLQYMLDGYEAGDVIDALVHKNWLHHSVYCLLPPKSQLLEYWKSNPSAIPDNVDRRLRKRLMLKKDLRKDDEYNQLARAFKISDVYAPLISSTTSPMTMIQNLNQGEIVYTTTDRVIGARILLYAPRKYYASTLSFTMTKCIIPFGKEVGRVPHSRFNVGTFPSIATPKCFVMSGVDIESIPNEFIKLFYQRVKSVHANILNDISPQIVSDMINRKRLRVHTPSDRRAAQLMHLPYHVKRGASHVDVYKVDVVDMLFEVVDVADGLRNVSRKLTMHTVPVCILEMLGIEIADYCIRQEDGMLTDWFLLLTMLSDGLTDRRTHCQYLINPSSVPPDVILNISITGFINRHTIDVMPDIYDFVKPIGAVLPKGSFKSTIMRVLDSISILGIQIMPRAHVVDSDEVGEQMEPTFEQAVMEIYKGIAGVDSLDDLIKWVLNSDLIPHDDRLGQLFQAFLPLAKDLLAPMARKFYDNSMSEGRLLTFAHADSELLNANYFGHLLRLKIPYITEVNLMIRKNREGGELFQLVLSYLYKMYATSAQPKWFGSLLRLLICPWLHMEKLIGEADPASTSAEIGWHIPREQLMQDGWCGCEDGFIPYVSIRAPRLVIEELMEKNWGQYHAQVIVTDQLVVGEPRRVSAKAVIKGNHLPVKLVSRFACFTLTAKYEMRLSCGHSTGRGAAYSARLAFRSDLA

Entity 2 Entity 2 polymer

Description description	RNA-directed RNA polymerase
Molecular weight formula_weight	142449.0 kDa
EC 编号 ec_number	2.7.7.48
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MSSMILTQFGPFIESISGITDQSNDVFEDAAKAFSMFTRSDVYKALDEIPFSDDAMLPIPPTIYTKPSHDSYYYIDALNRVRRKTYQGPDDVYVPNCSIVELLEPHETLTSYGRLSEAIENRAKDGDSQARIATTYGRIAESQARQIKAPLEKFVLALLVAEAGGSLYDPVLQKYDEIPDLSHNCPLWCFREICRHISGPLPDRAPYLYLSAGVFWLMSPRMTSAIPPLLSDLVNLAILQQTAGLDPSLVKLGVQICLHAAASSSYAWFILKTKSIFPQNTLHSMYESLEGGYCPNLEWLEPRSDYKFMYMGVMPLSAKYARSAPSNDKKARELGEKYGLSSVVGELRKRTKTYVKHDFASVRYIRDAMACTSGIFLVRTPTETVLQEYTQSPEIKVPIPQKDWTGPIGEIRILKDTTSSIARYLYRTWYLAAARMAAQPRTWDPLFQAIMRSQYVTARGGSGAALRESLYAINVSLPDFKGLPVKAATKIFQAAQLANLPFSHTSVAILADTSMGLRNQVQRRPRSIMPLNVPQQQVSAPHTLTADYINYHMNLSTTSGSAVIEKVIPLGVYASSPPNQSINIDISACDASITWDFFLSVIMAAIHEGVASSSIGKPFMGVPASIVNDESVVGVRAARPISGMQNMIQHLSKLYKRGFSYRVNDSFSPGNDFTHMTTTFPSGSTATSTEHTANNSTMMETFLTVWGPEHTDDPDVLRLMKSLTIQRNYVCQGDDGLMIIDGTTAGKVNSETIQKMLELISKYGEEFGWKYDIAYDGTAEYLKLYFIFGCRIPNLSRHPIVGKERANSSAEEPWPAILDQIMGVFFNGVHDGLQWQRWIRYSWALCCAFSRQRTMIGESVGYLQYPMWSFVYWGLPLVKAFGSDPWIFSWYMPTGDLGMYSWISLIRPLMTRWMVANGYVTDRCSPVFGNADYRRCFNELKLYQGYYMAQLPRNPKKSGRAAPREVREQFTQALSDYLMQNPELKSRVLRGRSEWEKYGAGIIHNPPSLFDVPHKWYQGAQEAAIATREELAEMDETLMRARRHSYSSFSKLLEAYLLVKWRMCEAREPSVDLRLPLCAGIDPLNSDPFLKMVSVGPMLQSTRKYFAQTLFMAKTVSGLDVNAIDSALLRLRTLGADKKALTAQLLMVGLQESEADALAGKIMLQDVNTVQLARVVNLAVPDTWMSLDFDSMFKHHVKLLPKDGRHLNTDIPPRMGWLRAILRFLGAGMVMTATGVAVDIYLEDIHGGGRSLGQRFMTWMRQEGRSA

Entity 3 Entity 3 polymer

Description description	transcript RNA
Molecular weight formula_weight	1263.8 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	C

AAGC

Entity 4 Entity 4 polymer

Description description	Lambda 1
Molecular weight formula_weight	122842.9 kDa
Source method src_method	nat
Copy count entity_count	10

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D,E,F,G,H,I,J,K,L,M

YQCHVCSAVLFSPLDLDAHVASHGLHGNMTLTSSDIQRHITEFISSWQNHPIVQVSADVENKKTAQLLHADTPRLVTWDAGLCTSFKIVPIVPAQVPQDVLAYTFFTSSYAIQSPFPEAAVSRIVVHTRWASNVDFDRDSSVIMAPPTENNIHLFKQLLNTETLSVRGANPLMFRANVLHMLLEFVLDNLYLNRHTGFSQDHTPFTEGANLRSLPGPDAEKWYSIMYPTRMGTPNVSKICNFVASCVRNRVGRFDRAQMMNGAMSEWVDVFETSDALTVSIRGRWMARLARMNINPTEIEWALTECAQGYVTVTSPYAPSVNRLMPYRISNAERQISQIIRIMNIGNNATVIQPVLQDISVLLQRISPLQIDPTIISNTMSTVSESTTQTLSPASSILGKLRPSNSDFSSFRVALAGWLYNGVVTTVIDDSSYPKDGGSVTSLENLWDFFILALALPLTTDPCAPVKAFMTLANMMVGFETIPMDNQIYTQSRRASAFSTPHTWPRCFMNIQLISPIDAPILRQWAEIIHRYWPNPSQIRYGAPNVFGSANLFTPPEVLLLPIDHQPANVTTPTLDFTNELTNWRARVCELMKNLVDNQRYQPGWTQSLVSSMRGTLDKLKLIKSMTPMYLQQLAPVELAVIAPMLPFPPFQVPYVRLDRDRVPTMVGVTRQSRDTITQPALSLSTTNTTVGVPLALDARAITVALLSGKYPPDLVTNVWYADAIYPMYADTEVFSNLQRDMITCEAVQTLVTLVAQISETQYPVDRYLDWIPSLRASAATAATFAEWVNTSMKTAFDLSDMLLEPLLSGDPRMTQLAIQYQQYNGRTFNIIPEMPGSVIADCVQLTAEVFNHEYNLFGIARGDIIIGRVQSTHLWSPLAPPPDLVFDRDTPGVHIFGRDCRISFGMNGAAPMIRDETGLMVPFEGNWIFPLALWQMNTRYFNQQFDAWIKTGELRIRIEMGAYPYMLHYYDPRQYANAWNLTSAWLEEITPTSIPSVPFMVPISSDHDISSAPAVQYIISTEYNDRSLFCTNSSSPQTIAGPDKHIPVERYNILTNPDAPPTQIQLPEVVDLYNVVTRYAYETPPITAVVMGVP

Entity 5 Entity 5 polymer

Description description	Lambda 1
Molecular weight formula_weight	19112.8 kDa
Source method src_method	nat
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	e,g,i,k,m

MKRIPRKTKGKSSGKGNDSTERADDGSSQLRDKQNNKAGPATTEPGTSNREQYKARPGIASVQRATESAEMPMKNNDEGTPDKKGNTKGDLVNEHSEAKDEADEATKKQAKDTDKSKAQVTYSDTGINNANELSRSGNVDNEGGSNQKPMSTRIAEATSAIVSKHPARVGLPPTASSGHG

Entity 6 Entity 6 polymer

Description description	RNA (60-MER)
Molecular weight formula_weight	19016.2 kDa
Source method src_method	nat
Copy count entity_count	8

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	N,O,P,Q,R,S,T,U

AUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAUAU

Entity 7 Entity 7 non-polymer

Description description	PHOSPHATE ION
Molecular weight formula_weight	95.0 kDa
Source method src_method	syn
Copy count entity_count	1

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.300 Å
Low-resolution limit resolution_low	— Å
R factor r_factor	—
R_work r_work	—
R_free r_free	—

Resolution resolution	3.30 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	102966

fit	/app/data/saxs_computation/el/7elh/7elh.fit
pddf	/app/data/pr_computation/el/7elh/7elh.pr.out
profile	/app/data/saxs_computation/el/7elh/7elh.dat

In situ structure of transcriptional enzyme complex and capsid shell protein of mammalian reovirus at initiation state

1. Protein Identity and Related Structures Protein Identity & Related Structures

Minor core protein mu2

State in the Current Structure

Other States of the Same Protein in the Database

RNA-directed RNA polymerase

State in the Current Structure

Other States of the Same Protein in the Database

Lambda 1

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

Entity 5 Entity 5 polymer

Polymer Polymer

Entity 6 Entity 6 polymer

Polymer Polymer

Entity 7 Entity 7 non-polymer

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)