7f0r

Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase subunit alpha

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

UniProt O52760

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 7 DNA 2 DNA-directed RNA polymerase subunit beta × 1 (Q51561) ;DNA-directed RNA polymerase subunit beta' ; × 1 (Q9HWC9) DNA-directed RNA polymerase subunit omega × 1 (Q9HTM1) Transcriptional factor SutA × 1 (Q9HTR9) RNA polymerase sigma factor RpoS × 1 (P45684) DNA (70-MER) × 1 DNA (70-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RPOA_PSEAE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 13–345; UniProt 1–333 Author chain B; PDBConstruct 13–345; UniProt 1–333

DNA-directed RNA polymerase subunit beta

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

UniProt Q51561

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 7 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (O52760) ;DNA-directed RNA polymerase subunit beta' ; × 1 (Q9HWC9) DNA-directed RNA polymerase subunit omega × 1 (Q9HTM1) Transcriptional factor SutA × 1 (Q9HTR9) RNA polymerase sigma factor RpoS × 1 (P45684) DNA (70-MER) × 1 DNA (70-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RPOB_PSEAE
Isoform —
PDB entities 2
Chains and sequence ranges Author chain C; PDBConstruct 3–1359; UniProt 1–1357

;DNA-directed RNA polymerase subunit beta' ;

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

UniProt Q9HWC9

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 7 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (O52760) DNA-directed RNA polymerase subunit beta × 1 (Q51561) DNA-directed RNA polymerase subunit omega × 1 (Q9HTM1) Transcriptional factor SutA × 1 (Q9HTR9) RNA polymerase sigma factor RpoS × 1 (P45684) DNA (70-MER) × 1 DNA (70-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RPOC_PSEAE
Isoform —
PDB entities 3
Chains and sequence ranges Author chain D; PDBConstruct 3–1400; UniProt 2–1399

DNA-directed RNA polymerase subunit omega

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

UniProt Q9HTM1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 7 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (O52760) DNA-directed RNA polymerase subunit beta × 1 (Q51561) ;DNA-directed RNA polymerase subunit beta' ; × 1 (Q9HWC9) Transcriptional factor SutA × 1 (Q9HTR9) RNA polymerase sigma factor RpoS × 1 (P45684) DNA (70-MER) × 1 DNA (70-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RPOZ_PSEAE
Isoform —
PDB entities 4
Chains and sequence ranges Author chain E; PDBConstruct 1–88; UniProt 1–88

Transcriptional factor SutA

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

UniProt Q9HTR9

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 7 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (O52760) DNA-directed RNA polymerase subunit beta × 1 (Q51561) ;DNA-directed RNA polymerase subunit beta' ; × 1 (Q9HWC9) DNA-directed RNA polymerase subunit omega × 1 (Q9HTM1) RNA polymerase sigma factor RpoS × 1 (P45684) DNA (70-MER) × 1 DNA (70-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name Q9HTR9_PSEAE
Isoform —
PDB entities 5
Chains and sequence ranges Author chain G; PDBConstruct 5–109; UniProt 1–105

RNA polymerase sigma factor RpoS

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

UniProt P45684

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 7 DNA 2 DNA-directed RNA polymerase subunit alpha × 2 (O52760) DNA-directed RNA polymerase subunit beta × 1 (Q51561) ;DNA-directed RNA polymerase subunit beta' ; × 1 (Q9HWC9) DNA-directed RNA polymerase subunit omega × 1 (Q9HTM1) Transcriptional factor SutA × 1 (Q9HTR9) DNA (70-MER) × 1 DNA (70-MER) × 1 ZINC ION × 2 MAGNESIUM ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RPOS_PSEAE
Isoform —
PDB entities 6
Chains and sequence ranges Author chain F; PDBConstruct 1–334; UniProt 1–334

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id7f0r
Deposition date deposition_date2021-06-06
Structure title titleCryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex
Keywords keywords;transcription initiation, Pseudomonas aeruginosa, SutA, transcription activation, sigmaS, RNA polymerase, RNAP beta lobe, TRANSLATION, TRANSCRIPTION-DNA complex ;; TRANSCRIPTION/DNA
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7f0r__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7f0r__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

7f0r__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)48.70 Å
Rg (electron density)47.54 Å
Total Rg47.78 Å
Atom count25722
Residues3544
Excluded volume448160 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 7f0r__assembly_1__model_1 nonameric (9) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (10)

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7. Citations (1)