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7hsc

HIGH RESOLUTION SOLUTION STRUCTURE OF THE HEAT SHOCK COGNATE-70 KD SUBSTRATE BINDING DOMAIN OBTAINED BY MULTIDIMENSIONAL NMR TECHNIQUES

Method: SOLUTION NMR Dmax: 69.8 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (HEAT SHOCK COGNATE 70 KD PROTEIN 1)

Rattus norvegicus

UniProt P63018

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 385–543 Fragment：SUBSTRATE BINDING DOMAIN No other associated polymer SOLUTION NMR NMR measurement conditions：pH 7；298 K；Ionic strength (raw mmCIF value) 50 mM SODIUM PHOSPHATE；Pressure 1 NMR sample composition：1.5 MM 15N OR 15N,13C LABELED PROTEIN 5%D20/95%H2O AND 100%D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7HSC Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1CKR HIGH RESOLUTION SOLUTION STRUCTURE OF THE HEAT SHOCK COGNATE-70 KD SUBSTRATE BINDING DOMAIN OBTAINED BY MULTIDIMENSIONAL NMR TECHNIQUES Deposited 1999-04-22 Different construct Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 385–542（158 aa） Fragment：SUBSTRATE BINDING DOMAIN
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 50 mM SODIUM PHOSPHATE；Pressure 1
NMR sample composition 1.5 MM 15N OR 15N,13C LABELED PROTEIN 5%D20/95%H2O AND 100%D2O
Resolution not provided
1UD0 CRYSTAL STRUCTURE OF THE C-TERMINAL 10-kDA SUBDOMAIN OF HSC70 Deposited 2003-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Chain B 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；298 K；ammonium sulfate, 2-propanol, sodium acetate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.45 Å R-free 0.309
1UD0 CRYSTAL STRUCTURE OF THE C-TERMINAL 10-kDA SUBDOMAIN OF HSC70 Deposited 2003-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Chain D 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；298 K；ammonium sulfate, 2-propanol, sodium acetate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.45 Å R-free 0.309
1UD0 CRYSTAL STRUCTURE OF THE C-TERMINAL 10-kDA SUBDOMAIN OF HSC70 Deposited 2003-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Chain B 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Chain C 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Chain D 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；298 K；ammonium sulfate, 2-propanol, sodium acetate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.45 Å R-free 0.309
1UD0 CRYSTAL STRUCTURE OF THE C-TERMINAL 10-kDA SUBDOMAIN OF HSC70 Deposited 2003-04-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Chain B 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Chain C 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Chain D 542–646（105 aa） Fragment：C-TERMINAL SUBDOMAIN
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NA SODIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；298 K；ammonium sulfate, 2-propanol, sodium acetate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.45 Å R-free 0.309

2 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name HSP7C_RAT
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–159； UniProt 385–543

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 7hsc

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 7hsc
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2. Structure Basics 2. Structure Basics

Entry ID entry_id7hsc
Deposition date deposition_date1999-05-03
Structure title titleHIGH RESOLUTION SOLUTION STRUCTURE OF THE HEAT SHOCK COGNATE-70 KD SUBSTRATE BINDING DOMAIN OBTAINED BY MULTIDIMENSIONAL NMR TECHNIQUES
Keywords keywordsMOLECULAR CHAPERONE, HSP70, PEPTIDE BINDING, PROTEIN FOLDING; MOLECULAR CHAPERONE
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier17.83 Å
Radius of gyration Rg (electron density) rg_electron16.74 Å
Forward intensity I(0) i06058440.00
Molecular weight molecular_weight17534.0 kDa
Excluded volume excluded_volume21903 ų
Envelope volume envelope_volume25899 ų
Hydration-shell volume shell_volume13751 ų
Envelope diameter envelope_diameter69.6 Å
Shell Rg shell_rg22.23 Å
Envelope Rg envelope_rg17.71 Å
Shape Rg shape_rg16.75 Å
Total Rg total_rg17.73 Å
Total atoms total_atoms2482
Residues n_residues158
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax69.8 Å
Rg (real space) rg_real17.96 Å
Rg uncertainty (real space) rg_real_error0.74 Å
I(0) (real space) i0_real6.0580e+06
I(0) uncertainty (real space) i0_real_error8.3390e+04
Rg (reciprocal space) rg_reciprocal17.94 Å
I(0) (reciprocal space) i0_reciprocal6058000.0000
Solution quality estimate total_estimate0.7809
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary20.8 Å
Skewness Skewness skewness0.657
Kurtosis Kurtosis kurtosis0.583
Angular range angular_range— – 0.4450 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha1437000.0000
Real-space data points n_real_points75
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.478; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.765; Smooth: 0.946
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd7hsca_
Class classb — All beta proteins
Fold Fold foldb.130 — Heat shock protein 70kD (HSP70), peptide-binding domain
Superfamily Superfamily superfamilyb.130.1 — Heat shock protein 70kD (HSP70), peptide-binding domain
Family Family familyb.130.1.1 — Heat shock protein 70kD (HSP70), peptide-binding domain

CATH v4.4 (1 domains)

Domain ID domain_id7hscA00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology34 — Substrate Binding Domain Of DNAk; Chain A, domain 1
Homologous superfamily homologous superfamily10 — Substrate Binding Domain Of DNAk; Chain A, domain 1
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8. Citations (1)

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9. Files and Curves (10)