← Back to search
7nob

Structure of the mature RSV CA lattice: Group II, hexamer-hexamer interface, class 2'6

Method: ELECTRON MICROSCOPY Dmax: 139.5 Å Quality: GOOD
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Capsid protein p27, alternate cleaved 1

Rous sarcoma virus (strain Prague C)

UniProt P03322

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 10 PDB declaration: decameric（10） Consistent with protein copy count Chain A； UniProt 240–468 Chain B； UniProt 240–468 Chain C； UniProt 240–468 Chain D； UniProt 240–468 Chain E； UniProt 240–468 Chain F； UniProt 240–468 Chain G； UniProt 240–468 Chain H； UniProt 240–468 Chain I； UniProt 240–468 Chain J； UniProt 240–468 Not recorded No other associated polymer ELECTRON MICROSCOPY cryo-EM buffer：pH 6.2 cryo-EM vitrification conditions：Cryogen ETHANE；2.5 seconds blotting time Resolution 6.70 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7NOB Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1A6S M-DOMAIN FROM GAG POLYPROTEIN OF ROUS SARCOMA VIRUS, NMR, 20 STRUCTURES Deposited 1998-03-02 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–87（86 aa） Fragment：M-DOMAIN
Mutation：M1G No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6；308 K；Ionic strength (raw mmCIF value) 100 mM；Pressure 1
NMR sample composition H20
Resolution not provided
1EM9 ROUS SARCOMA VIRUS CAPSID PROTEIN: N-TERMINAL DOMAIN Deposited 2000-03-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 240–393（154 aa） Fragment：N-TERMINAL DOMAIN
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 9.1；277 K；Boric acid/Potassium Hydroxide, PEG 6000, Magnesium Nitrate, pH 9.1, VAPOR DIFFUSION, temperature 277K
Resolution 2.05 Å R-free 0.271
1EM9 ROUS SARCOMA VIRUS CAPSID PROTEIN: N-TERMINAL DOMAIN Deposited 2000-03-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 240–393（154 aa） Fragment：N-TERMINAL DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 9.1；277 K；Boric acid/Potassium Hydroxide, PEG 6000, Magnesium Nitrate, pH 9.1, VAPOR DIFFUSION, temperature 277K
Resolution 2.05 Å R-free 0.271
1EOQ ROUS SARCOMA VIRUS CAPSID PROTEIN: C-TERMINAL DOMAIN Deposited 2000-03-23 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 394–488（95 aa） Fragment：C-TERMINAL DOMAIN
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 4.9；298 K；Ionic strength (raw mmCIF value) 50 mM NaCl；Pressure ambient
NMR measurement conditions pH 4.9；298 K；Ionic strength (raw mmCIF value) 50 mM NaCl；Pressure ambient
NMR measurement conditions pH 4.9；298 K；Ionic strength (raw mmCIF value) 50 mM NaCl；Pressure ambient
NMR sample composition U-15N RSV CA(155-249) (used for the 3D_15N-SEPARATED_NOESY, HNHA, HNHB expts) and U-15N/U-13C RSV CA(155-249) (used for the 3D_13C-SEPARATED_NOESY expt) both suspended in the same buffer (50 mM Sodium phosphate pH 4.9 50 mM NaCl 1 mM EDTA 1 mM DTT) | 90% H20, 10%D20
NMR sample composition U-15N RSV CA(155-249) 50 mM Sodium phosphate pH 4.9 50 mM NaCl 1 mM EDTA 1 mM DTT | 90% H20, 10%D20
NMR sample composition U-15N,13C RSV CA(155-249) 50 mM Sodium phosphate pH 4.9 50 mM NaCl 1 mM EDTA 1 mM DTT | 90% H20, 10%D20
Resolution not provided
1P7N Dimeric Rous Sarcoma virus Capsid protein structure with an upstream 25-amino acid residue extension of C-terminal of Gag p10 protein Deposited 2003-05-02 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 215–386（172 aa） Fragment：N-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；10 mM HEPES-sodium buffer and 0.8M potassium sodium tartrate tetrahydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.298
2IHX Solution Structure of the Rous Sarcoma Virus Nucleocapsid Protein:uPsi RNA Packaging Signal Complex Deposited 2006-09-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric
Chain A 503–563（61 aa） Fragment：Nucleocapsid domain (residues 503-563)
Not recorded ZN ZINC ION × 2 SOLUTION NMR
NMR measurement conditions pH 7；308 K；Ionic strength (raw mmCIF value) 5 mM NaCl, 0.1 mM ZnCl2；Pressure ambient
NMR sample composition 1.0 mM unlabeled nucleocapsid protein, 1.0 mM A-selectively-protonated uPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O | 100% D2O
NMR sample composition 1.2 mM unlabeled nucleocapsid protein, 1.2 mM G-selectively-protonated uPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O | 100% D2O
NMR sample composition 0.8 mM unlabeled nucleocapsid protein, 0.8 mM U-selectively-protonated uPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O | 100% D2O
NMR sample composition 0.8 mM unlabeled nucleocapsid protein, 0.8 mM C-selectively-protonated uPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O | 100% D2O
NMR sample composition 1.2 mM unlabeled nucleocapsid protein, 1.2 mM unlabeled uPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0, 5mM NaCl, 0.1 mM ZnCl2, 100% D2O | 100% D2O
NMR sample composition 1.2 mM 15N, 13C-labeled nucleocapsid protein, 1.2 mM unlabeled uPsi RNA, 5 mM Tris-d11-HCl buffer, pH 7.0; 5mM NaCl, 0.1 mM ZnCl2, 100% D2O | 100% D2O
Resolution not provided
2RSP STRUCTURE OF THE ASPARTIC PROTEASE FROM ROUS SARCOMA RETROVIRUS REFINED AT 2 ANGSTROMS RESOLUTION Deposited 1989-10-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 578–701（124 aa）
Chain B 578–701（124 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.00 Å
2X8Q Cryo-EM 3D model of the icosahedral particle composed of Rous sarcoma virus capsid protein pentamers Deposited 2010-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 60 PDB declaration: 60-MERIC
Chain A 240–465（226 aa） Fragment：RESIDUES 240-465
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL；pH 5；0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL
cryo-EM vitrification conditions Cryogen ETHANE；CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 85, INSTRUMENT- VITROBOT MARK IV, METHOD- BLOT FOR 5 SECONDS BEFORE PLUNGING,
Resolution 18.30 Å
2X8Q Cryo-EM 3D model of the icosahedral particle composed of Rous sarcoma virus capsid protein pentamers Deposited 2010-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 240–465（226 aa） Fragment：RESIDUES 240-465
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL；pH 5；0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL
cryo-EM vitrification conditions Cryogen ETHANE；CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 85, INSTRUMENT- VITROBOT MARK IV, METHOD- BLOT FOR 5 SECONDS BEFORE PLUNGING,
Resolution 18.30 Å
2X8Q Cryo-EM 3D model of the icosahedral particle composed of Rous sarcoma virus capsid protein pentamers Deposited 2010-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric
Chain A 240–465（226 aa） Fragment：RESIDUES 240-465
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL；pH 5；0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL
cryo-EM vitrification conditions Cryogen ETHANE；CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 85, INSTRUMENT- VITROBOT MARK IV, METHOD- BLOT FOR 5 SECONDS BEFORE PLUNGING,
Resolution 18.30 Å
2X8Q Cryo-EM 3D model of the icosahedral particle composed of Rous sarcoma virus capsid protein pentamers Deposited 2010-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 240–465（226 aa） Fragment：RESIDUES 240-465
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL；pH 5；0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL
cryo-EM vitrification conditions Cryogen ETHANE；CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 85, INSTRUMENT- VITROBOT MARK IV, METHOD- BLOT FOR 5 SECONDS BEFORE PLUNGING,
Resolution 18.30 Å
2X8Q Cryo-EM 3D model of the icosahedral particle composed of Rous sarcoma virus capsid protein pentamers Deposited 2010-03-11 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 240–465（226 aa） Fragment：RESIDUES 240-465
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL；pH 5；0.1M CITRIC ACID, 5MM MOPS/KOH, 725MM NACL, 0.25MM NA AZIDE, 0.125MM TCEP-HCL
cryo-EM vitrification conditions Cryogen ETHANE；CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 85, INSTRUMENT- VITROBOT MARK IV, METHOD- BLOT FOR 5 SECONDS BEFORE PLUNGING,
Resolution 18.30 Å
3G0V Crystal structure of the C-terminal domain from the Rous Sarcoma Virus capsid protein: mutant D179A Deposited 2009-01-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 389–465（77 aa） Fragment：C-terminal domain, UNP residues 389-465
Mutation：D179A NO3 NITRATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.3；291 K；0.2M Succinic acid/KOH, pH4.3, 24% PEG8000, 1M Sodium Nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 2.00 Å R-free 0.228
3G1G Crystal structure of the C-terminal domain from the Rous Sarcoma Virus capsid protein: High pH Deposited 2009-01-29 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 390–476（87 aa） Fragment：C-terminal domain, UNP residues 390-476
Chain B 390–476（87 aa） Fragment：C-terminal domain, UNP residues 390-476
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 10.3；291 K；0.2M Beta-Alanine/KOH, pH10.3, 10-25% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 2.01 Å R-free 0.257
3G1G Crystal structure of the C-terminal domain from the Rous Sarcoma Virus capsid protein: High pH Deposited 2009-01-29 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 390–476（87 aa） Fragment：C-terminal domain, UNP residues 390-476
Chain B 390–476（87 aa） Fragment：C-terminal domain, UNP residues 390-476
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 10.3；291 K；0.2M Beta-Alanine/KOH, pH10.3, 10-25% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 2.01 Å R-free 0.257
3G1I Crystal structure of the C-terminal domain of the Rous Sarcoma Virus capsid protein: Intermediate pH Deposited 2009-01-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 389–465（77 aa） Fragment：C-terminal domain, UNP residues 389-465
Chain B 389–465（77 aa） Fragment：C-terminal domain, UNP residues 389-465
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；0.2M Bis-tris propane/HCl, pH8.5, 0.75-1.85M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 2.10 Å R-free 0.245
3G21 Crystal structure of the C-terminal domain of the Rous Sarcoma Virus capsid protein: Low pH Deposited 2009-01-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 389–465（77 aa） Fragment：C-terminal domain, UNP residues 389-465
Not recorded NO3 NITRATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.3；291 K；0.20M Succinic acid/KOH, pH4.3, 13-18% PEG8000, 0.75M Magnesium Nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 0.90 Å R-free 0.142
3G26 Crystal structure of the C-terminal domain of the Rous Sarcoma Virus capsid protein: Mutant A184C Deposited 2009-01-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 389–465（77 aa） Fragment：C-terminal domain, UNP residues 389-465
Mutation：A184C MLA MALONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 3.7；291 K；0.1M Malic acid/KOH, pH3.7, 1.4 M Malonic acid/KOH, pH3.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.55 Å R-free 0.214
3G28 Crystal structure of the C-terminal domain of the Rous Sarcoma Virus capsid protein: mutant D179N, low pH Deposited 2009-01-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 389–465（77 aa） Fragment：C-terminal domain, UNP residues 389-465
Mutation：D179N NO3 NITRATE ION × 4 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 3.7；291 K；0.2M Formic acid/KOH, pH3.7, 12% PEG8000, 0.25M Ammonium Nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.64 Å R-free 0.200
3G29 Crystal structure of the C-terminal domain of the Rous Sarcoma Virus capsid protein: D179N mutant, neutral pH Deposited 2009-01-30 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 389–465（77 aa） Fragment：C-terminal domain, UNP residues 389-465
Mutation：D179N No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.9；291 K；0.20M Tris/HCl, pH7.9, 1.5M Ammonium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 2.50 Å R-free 0.322
3G29 Crystal structure of the C-terminal domain of the Rous Sarcoma Virus capsid protein: D179N mutant, neutral pH Deposited 2009-01-30 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 389–465（77 aa） Fragment：C-terminal domain, UNP residues 389-465
Mutation：D179N No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.9；291 K；0.20M Tris/HCl, pH7.9, 1.5M Ammonium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 2.50 Å R-free 0.322
5A9E Cryo-electron tomography and subtomogram averaging of Rous-Sarcoma- Virus deltaMBD virus-like particles Deposited 2015-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 84–577（494 aa）
Chain B 84–577（494 aa）
Chain C 84–577（494 aa）
Chain D 84–577（494 aa）
Chain E 84–577（494 aa）
Chain F 84–577（494 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP；pH 6.5；MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP
cryo-EM vitrification conditions Cryogen ETHANE；DEGASSED C-FLAT 2 2-3C GRIDS WERE GLOW DISCHARGED FOR 30 SECONDS AT 20 MA. VIRUS SOLUTION WAS DILUTED IN OBS CONTAINING 10NM COLLOIDAL GOLD. 2.5UL OF THIS MIXTURE WAS APPLIED TO A GRID. BLOTTING TIME 2.5 SECONDS.
Resolution 7.70 Å
5A9E Cryo-electron tomography and subtomogram averaging of Rous-Sarcoma- Virus deltaMBD virus-like particles Deposited 2015-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain B 84–577（494 aa）
Chain C 84–577（494 aa）
Chain D 84–577（494 aa）
Chain E 84–577（494 aa）
Chain K 84–577（494 aa）
Chain R 84–577（494 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP；pH 6.5；MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP
cryo-EM vitrification conditions Cryogen ETHANE；DEGASSED C-FLAT 2 2-3C GRIDS WERE GLOW DISCHARGED FOR 30 SECONDS AT 20 MA. VIRUS SOLUTION WAS DILUTED IN OBS CONTAINING 10NM COLLOIDAL GOLD. 2.5UL OF THIS MIXTURE WAS APPLIED TO A GRID. BLOTTING TIME 2.5 SECONDS.
Resolution 7.70 Å
5A9E Cryo-electron tomography and subtomogram averaging of Rous-Sarcoma- Virus deltaMBD virus-like particles Deposited 2015-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain B 84–577（494 aa）
Chain C 84–577（494 aa）
Chain D 84–577（494 aa）
Chain E 84–577（494 aa）
Chain L 84–577（494 aa）
Chain M 84–577（494 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP；pH 6.5；MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP
cryo-EM vitrification conditions Cryogen ETHANE；DEGASSED C-FLAT 2 2-3C GRIDS WERE GLOW DISCHARGED FOR 30 SECONDS AT 20 MA. VIRUS SOLUTION WAS DILUTED IN OBS CONTAINING 10NM COLLOIDAL GOLD. 2.5UL OF THIS MIXTURE WAS APPLIED TO A GRID. BLOTTING TIME 2.5 SECONDS.
Resolution 7.70 Å
5A9E Cryo-electron tomography and subtomogram averaging of Rous-Sarcoma- Virus deltaMBD virus-like particles Deposited 2015-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain B 84–577（494 aa）
Chain C 84–577（494 aa）
Chain D 84–577（494 aa）
Chain E 84–577（494 aa）
Chain G 84–577（494 aa）
Chain N 84–577（494 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP；pH 6.5；MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP
cryo-EM vitrification conditions Cryogen ETHANE；DEGASSED C-FLAT 2 2-3C GRIDS WERE GLOW DISCHARGED FOR 30 SECONDS AT 20 MA. VIRUS SOLUTION WAS DILUTED IN OBS CONTAINING 10NM COLLOIDAL GOLD. 2.5UL OF THIS MIXTURE WAS APPLIED TO A GRID. BLOTTING TIME 2.5 SECONDS.
Resolution 7.70 Å
5A9E Cryo-electron tomography and subtomogram averaging of Rous-Sarcoma- Virus deltaMBD virus-like particles Deposited 2015-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain B 84–577（494 aa）
Chain C 84–577（494 aa）
Chain D 84–577（494 aa）
Chain E 84–577（494 aa）
Chain H 84–577（494 aa）
Chain O 84–577（494 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP；pH 6.5；MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP
cryo-EM vitrification conditions Cryogen ETHANE；DEGASSED C-FLAT 2 2-3C GRIDS WERE GLOW DISCHARGED FOR 30 SECONDS AT 20 MA. VIRUS SOLUTION WAS DILUTED IN OBS CONTAINING 10NM COLLOIDAL GOLD. 2.5UL OF THIS MIXTURE WAS APPLIED TO A GRID. BLOTTING TIME 2.5 SECONDS.
Resolution 7.70 Å
5A9E Cryo-electron tomography and subtomogram averaging of Rous-Sarcoma- Virus deltaMBD virus-like particles Deposited 2015-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 6 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain B 84–577（494 aa）
Chain C 84–577（494 aa）
Chain D 84–577（494 aa）
Chain E 84–577（494 aa）
Chain I 84–577（494 aa）
Chain P 84–577（494 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP；pH 6.5；MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP
cryo-EM vitrification conditions Cryogen ETHANE；DEGASSED C-FLAT 2 2-3C GRIDS WERE GLOW DISCHARGED FOR 30 SECONDS AT 20 MA. VIRUS SOLUTION WAS DILUTED IN OBS CONTAINING 10NM COLLOIDAL GOLD. 2.5UL OF THIS MIXTURE WAS APPLIED TO A GRID. BLOTTING TIME 2.5 SECONDS.
Resolution 7.70 Å
5A9E Cryo-electron tomography and subtomogram averaging of Rous-Sarcoma- Virus deltaMBD virus-like particles Deposited 2015-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain B 84–577（494 aa）
Chain C 84–577（494 aa）
Chain D 84–577（494 aa）
Chain E 84–577（494 aa）
Chain J 84–577（494 aa）
Chain Q 84–577（494 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP；pH 6.5；MES PH6.5, 100MM NACL, 2UM ZNCL2, 2MM TCEP
cryo-EM vitrification conditions Cryogen ETHANE；DEGASSED C-FLAT 2 2-3C GRIDS WERE GLOW DISCHARGED FOR 30 SECONDS AT 20 MA. VIRUS SOLUTION WAS DILUTED IN OBS CONTAINING 10NM COLLOIDAL GOLD. 2.5UL OF THIS MIXTURE WAS APPLIED TO A GRID. BLOTTING TIME 2.5 SECONDS.
Resolution 7.70 Å
7NO0 Structure of the mature RSV CA lattice: T=1 CA icosahedron Deposited 2021-02-25 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 60 PDB declaration: 60-meric
Chain A 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；blot time= 2.5 s blot force= 0
Resolution 3.10 Å
7NO1 Structure of the mature RSV CA lattice: T=3 CA icosahedron Deposited 2021-02-25 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 180 PDB declaration: 180-meric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；blot time = 2.5s blot force = 0
Resolution 7.60 Å
7NO2 Structure of the mature RSV CA lattice: hexamer derived from tubes (C2-symmetric) Deposited 2021-02-25 Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 4.30 Å
7NO3 Structure of the mature RSV CA lattice: pentamer derived from polyhedral VLPs Deposited 2021-02-25 Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 5.80 Å
7NO4 Structure of the mature RSV CA lattice: hexamer with 3 adjacent pentamers (C3 symmetric) Deposited 2021-02-25 Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.50 Å
7NO5 Structure of the mature RSV CA lattice: hexamer with 2 adjacent pentamers (C2 symmetric) Deposited 2021-02-25 Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.40 Å
7NO6 Structure of the mature RSV CA lattice: Group I, pentamer-hexamer interface, class 1 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 6.00 Å
7NO7 Structure of the mature RSV CA lattice: Group I, pentamer-hexamer interface, class 1"2 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.50 Å
7NO8 Structure of the mature RSV CA lattice: Group I, pentamer-hexamer interface, class 1"6 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.90 Å
7NO9 Structure of the mature RSV CA lattice: Group I, pentamer-pentamer interface, class 1'1 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.60 Å
7NOA Structure of the mature RSV CA lattice: Group II, hexamer-hexamer interface, class 6 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 6.40 Å
7NOC Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 3'3 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.80 Å
7NOD Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 3'4 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.80 Å
7NOE Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 3'5 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.50 Å
7NOF Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 4'4 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.60 Å
7NOG Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 4'5 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.80 Å
7NOH Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 5'5 Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.10 Å
7NOI Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 3'Alpha Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.20 Å
7NOJ Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 3'Beta Deposited 2021-02-25 Parsed fields agree Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 6.70 Å
7NOK Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 3'Gamma Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 9.10 Å
7NOL Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 4'Alpha Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 8.20 Å
7NOM Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 4'Beta Deposited 2021-02-25 Parsed fields agree Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 6.70 Å
7NON Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 4'Gamma Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 6.80 Å
7NOO Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 5'Alpha Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.90 Å
7NOP Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 5'Beta Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 7.80 Å
7NOQ Structure of the mature RSV CA lattice: Group IV, hexamer-hexamer interface, class 5'Gamma Deposited 2021-02-25 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric
Chain A 240–468（229 aa）
Chain B 240–468（229 aa）
Chain C 240–468（229 aa）
Chain D 240–468（229 aa）
Chain E 240–468（229 aa）
Chain F 240–468（229 aa）
Chain G 240–468（229 aa）
Chain H 240–468（229 aa）
Chain I 240–468（229 aa）
Chain J 240–468（229 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.2
cryo-EM vitrification conditions Cryogen ETHANE；2.5 seconds blotting time
Resolution 6.50 Å

41 other PDB entries and 54 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name GAG_RSVP
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–229； UniProt 240–468 Author chain B； PDBConstruct 1–229； UniProt 240–468 Author chain C； PDBConstruct 1–229； UniProt 240–468 Author chain D； PDBConstruct 1–229； UniProt 240–468 Author chain E； PDBConstruct 1–229； UniProt 240–468 Author chain F； PDBConstruct 1–229； UniProt 240–468 Author chain G； PDBConstruct 1–229； UniProt 240–468 Author chain H； PDBConstruct 1–229； UniProt 240–468 Author chain I； PDBConstruct 1–229； UniProt 240–468 Author chain J； PDBConstruct 1–229； UniProt 240–468

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 7nob

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 7nob
Download Download
▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id7nob
Deposition date deposition_date2021-02-25
Structure title titleStructure of the mature RSV CA lattice: Group II, hexamer-hexamer interface, class 2'6
Keywords keywordsRetrovirus, Rous sarcoma virus, capsid protein, IP6, VIRAL PROTEIN; VIRAL PROTEIN
Experimental Method methodELECTRON MICROSCOPY
▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier41.05 Å
Radius of gyration Rg (electron density) rg_electron41.02 Å
Forward intensity I(0) i0217520000.00
Molecular weight molecular_weight72949.0 kDa
Excluded volume excluded_volume71508 ų
Envelope volume envelope_volume207020 ų
Hydration-shell volume shell_volume45508 ų
Envelope diameter envelope_diameter145.7 Å
Shell Rg shell_rg43.00 Å
Envelope Rg envelope_rg38.57 Å
Shape Rg shape_rg41.01 Å
Total Rg total_rg41.16 Å
Total atoms total_atoms5208
Residues n_residues1302
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax139.5 Å
Rg (real space) rg_real41.09 Å
Rg uncertainty (real space) rg_real_error1.51 Å
I(0) (real space) i0_real2.1750e+08
I(0) uncertainty (real space) i0_real_error3.6690e+06
Rg (reciprocal space) rg_reciprocal41.05 Å
I(0) (reciprocal space) i0_reciprocal217500000.0000
Solution quality estimate total_estimate0.8585
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary51.4 Å
Skewness Skewness skewness0.387
Kurtosis Kurtosis kurtosis-0.026
Angular range angular_range— – 0.1900 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha16850000.0000
Real-space data points n_real_points39
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.818; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.701
▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (1)

▶

8. Citations (1)

▶

9. Files and Curves (10)