SAXSdb — 7wi3

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Modulator of FtsH protease HflC

Escherichia coli K-12

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 48	Modulator of FtsH protease HflK × 12 （P0ABC7） ATP-dependent zinc metalloprotease FtsH × 24 （P0AAI3）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	HFLC_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–334； UniProt 1–334 Author chain C； PDBConstruct 1–334； UniProt 1–334 Author chain D； PDBConstruct 1–334； UniProt 1–334 Author chain M； PDBConstruct 1–334； UniProt 1–334 Author chain O； PDBConstruct 1–334； UniProt 1–334 Author chain P； PDBConstruct 1–334； UniProt 1–334 Author chain Y； PDBConstruct 1–334； UniProt 1–334 Author chain Z； PDBConstruct 1–334； UniProt 1–334 Author chain c； PDBConstruct 1–334； UniProt 1–334 Author chain d； PDBConstruct 1–334； UniProt 1–334 Author chain e； PDBConstruct 1–334； UniProt 1–334 Author chain f； PDBConstruct 1–334； UniProt 1–334

Modulator of FtsH protease HflK

Escherichia coli K-12

UniProt P0ABC7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 48	Modulator of FtsH protease HflC × 12 （P0ABC3） ATP-dependent zinc metalloprotease FtsH × 24 （P0AAI3）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	HFLK_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–419； UniProt 1–419 Author chain F； PDBConstruct 1–419； UniProt 1–419 Author chain G； PDBConstruct 1–419； UniProt 1–419 Author chain N； PDBConstruct 1–419； UniProt 1–419 Author chain R； PDBConstruct 1–419； UniProt 1–419 Author chain S； PDBConstruct 1–419； UniProt 1–419 Author chain a； PDBConstruct 1–419； UniProt 1–419 Author chain b； PDBConstruct 1–419； UniProt 1–419 Author chain i； PDBConstruct 1–419； UniProt 1–419 Author chain j； PDBConstruct 1–419； UniProt 1–419 Author chain k； PDBConstruct 1–419； UniProt 1–419 Author chain l； PDBConstruct 1–419； UniProt 1–419

ATP-dependent zinc metalloprotease FtsH

Escherichia coli K-12

UniProt P0AAI3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 48	Modulator of FtsH protease HflC × 12 （P0ABC3） Modulator of FtsH protease HflK × 12 （P0ABC7）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FTSH_ECOLI
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain E； PDBConstruct 1–644； UniProt 1–644 Author chain H； PDBConstruct 1–644； UniProt 1–644 Author chain I； PDBConstruct 1–644； UniProt 1–644 Author chain J； PDBConstruct 1–644； UniProt 1–644 Author chain K； PDBConstruct 1–644； UniProt 1–644 Author chain L； PDBConstruct 1–644； UniProt 1–644 Author chain Q； PDBConstruct 1–644； UniProt 1–644 Author chain T； PDBConstruct 1–644； UniProt 1–644 Author chain U； PDBConstruct 1–644； UniProt 1–644 Author chain V； PDBConstruct 1–644； UniProt 1–644 Author chain W； PDBConstruct 1–644； UniProt 1–644 Author chain X； PDBConstruct 1–644； UniProt 1–644 Author chain g； PDBConstruct 1–644； UniProt 1–644 Author chain h； PDBConstruct 1–644； UniProt 1–644 Author chain m； PDBConstruct 1–644； UniProt 1–644 Author chain n； PDBConstruct 1–644； UniProt 1–644 Author chain o； PDBConstruct 1–644； UniProt 1–644 Author chain p； PDBConstruct 1–644； UniProt 1–644 Author chain q； PDBConstruct 1–644； UniProt 1–644 Author chain r； PDBConstruct 1–644； UniProt 1–644 Author chain s； PDBConstruct 1–644； UniProt 1–644 Author chain t； PDBConstruct 1–644； UniProt 1–644 Author chain u； PDBConstruct 1–644； UniProt 1–644 Author chain v； PDBConstruct 1–644； UniProt 1–644

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7wi3
Deposition date deposition_date	2022-01-02
Structure title title	Cryo-EM structure of E.Coli FtsH-HflkC AAA protease complex
Keywords keywords	complex, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7wi3__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7wi3__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

7wi3__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	82.62 Å
Rg (electron density)	82.76 Å
Total Rg	82.86 Å
Atom count	69304
Residues	8688
Excluded volume	1234400 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`7wi3__assembly_1__model_1`	48-meric （48）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	Modulator of FtsH protease HflC
Molecular weight formula_weight	37703.9 kDa
Source method src_method	man
Copy count entity_count	12

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,C,D,Y,c,e,Z,d,f,M,O,P
Atom count atom_count	69304

MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNKPLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSRYYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSAGTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTEVSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGDAEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR

Source Source

Source organism organism	Escherichia coli K-12
NCBI taxonomy ID ncbi_taxonomy_id	83333
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	Modulator of FtsH protease HflK
Molecular weight formula_weight	45598.8 kDa
Source method src_method	man
Copy count entity_count	12

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,F,G,a,i,k,b,j,l,N,R,S

MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVEMNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGDIMDQRRANAQRNDYQRQGE

Source Source

Source organism organism	Escherichia coli K-12
NCBI taxonomy ID ncbi_taxonomy_id	83333
Host organism host_organism	Escherichia coli

Entity 3 Entity 3 polymer

Description description	ATP-dependent zinc metalloprotease FtsH
Molecular weight formula_weight	70797.0 kDa
EC 编号 ec_number	3.4.24.-
Source method src_method	man
Copy count entity_count	24

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E,H,I,J,K,L,g,m,o,q,s,u,h,n,p,r,t,v,Q,T,U,V,W,X

MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNNDQVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASIFISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEAKEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVHMRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKIMMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLPEGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWGFSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTDNMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRPVDEPRTPNPGNTMSEQLGDK

Source Source

Source organism organism	Escherichia coli K-12
NCBI taxonomy ID ncbi_taxonomy_id	83333
Host organism host_organism	Escherichia coli

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	4.00 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	26863

Cryo-EM structure of E.Coli FtsH-HflkC AAA protease complex

1. Protein Identity and Related Structures Protein Identity & Related Structures

Modulator of FtsH protease HflC

State in the Current Structure

Other States of the Same Protein in the Database

Modulator of FtsH protease HflK

State in the Current Structure

Other States of the Same Protein in the Database

ATP-dependent zinc metalloprotease FtsH

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

7wi3__assembly_1__model_1

7wi3__assembly_1__model_1 | I(q)

7wi3__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

7. Citations (1)

Cryo-EM structure of E.Coli FtsH-HflkC AAA protease complex

1. Protein Identity and Related Structures Protein Identity & Related Structures

Modulator of FtsH protease HflC

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Modulator of FtsH protease HflK

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

ATP-dependent zinc metalloprotease FtsH

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

7wi3__assembly_1__model_1

7wi3__assembly_1__model_1 | I(q)

7wi3__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

7. Citations (1)