8c93

Cryo-EM captures early ribosome assembly in action

Method: ELECTRON MICROSCOPY

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

50S ribosomal protein L3

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL3_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain D； PDBConstruct 1–209； UniProt 1–209

50S ribosomal protein L4

OrganismNot specified

UniProt P60723

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL4_ECOLI
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain E； PDBConstruct 1–201； UniProt 1–201

50S ribosomal protein L13

OrganismNot specified

UniProt P0AA10

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL13_ECOLI
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain J； PDBConstruct 1–142； UniProt 1–142

50S ribosomal protein L14

OrganismNot specified

UniProt P0ADY3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL14_ECOLI
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain K； PDBConstruct 1–123； UniProt 1–123

50S ribosomal protein L17

OrganismNot specified

UniProt P0AG44

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL17_ECOLI
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain N； PDBConstruct 1–127； UniProt 1–127

50S ribosomal protein L20

OrganismNot specified

UniProt P0A7L3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL20_ECOLI
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain Q； PDBConstruct 1–118； UniProt 1–118

50S ribosomal protein L21

OrganismNot specified

UniProt P0AG48

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL21_ECOLI
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain R； PDBConstruct 1–103； UniProt 1–103

50S ribosomal protein L22

OrganismNot specified

UniProt P61175

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL22_ECOLI
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain S； PDBConstruct 1–110； UniProt 1–110

50S ribosomal protein L23

OrganismNot specified

UniProt P0ADZ0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL23_ECOLI
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain T； PDBConstruct 1–100； UniProt 1–100

50S ribosomal protein L24

OrganismNot specified

UniProt P60624

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL24_ECOLI
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain U； PDBConstruct 1–104； UniProt 1–104

50S ribosomal protein L29

OrganismNot specified

UniProt P0A7M6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL29_ECOLI
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain Y； PDBConstruct 1–63； UniProt 1–63

50S ribosomal protein L32

OrganismNot specified

UniProt P0A7N4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL32_ECOLI
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain 0； PDBConstruct 1–57； UniProt 1–57

50S ribosomal protein L34

OrganismNot specified

UniProt P0A7P5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL34_ECOLI
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain 2； PDBConstruct 1–46； UniProt 1–46

50S ribosomal protein L30

OrganismNot specified

UniProt P0AG51

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L15 × 1 （P02413） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL30_ECOLI
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain Z； PDBConstruct 1–59； UniProt 1–59

50S ribosomal protein L15

OrganismNot specified

UniProt P02413

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L19 × 1 （P0A7K6）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL15_ECOLI
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain L； PDBConstruct 1–144； UniProt 1–144

50S ribosomal protein L19

OrganismNot specified

UniProt P0A7K6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 16 RNA 1	23S rRNA × 1 50S ribosomal protein L3 × 1 （P60438） 50S ribosomal protein L4 × 1 （P60723） 50S ribosomal protein L13 × 1 （P0AA10） 50S ribosomal protein L14 × 1 （P0ADY3） 50S ribosomal protein L17 × 1 （P0AG44） 50S ribosomal protein L20 × 1 （P0A7L3） 50S ribosomal protein L21 × 1 （P0AG48） 50S ribosomal protein L22 × 1 （P61175） 50S ribosomal protein L23 × 1 （P0ADZ0） 50S ribosomal protein L24 × 1 （P60624） 50S ribosomal protein L29 × 1 （P0A7M6） 50S ribosomal protein L32 × 1 （P0A7N4） 50S ribosomal protein L34 × 1 （P0A7P5） 50S ribosomal protein L30 × 1 （P0AG51） 50S ribosomal protein L15 × 1 （P02413）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL19_ECOLI
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain P； PDBConstruct 1–115； UniProt 1–115

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	8c93
Deposition date deposition_date	2023-01-21
Structure title title	Cryo-EM captures early ribosome assembly in action
Keywords keywords	ribosome, ribosome assembly, ribosome biogenesis, total reconstitution, RNA, ribosomal protein.; RIBOSOME
Experimental Method method	ELECTRON MICROSCOPY

▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8c93__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8c93__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8c93__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	64.86 Å
Rg (electron density)	64.80 Å
Total Rg	64.90 Å
Atom count	50320
Residues	3397
Excluded volume	758640 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8c93__assembly_1__model_1`	heptadecameric （17）	Success	4.1.3-1-20251215 (887e7ef)	View Download

▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (17)

Entity 1 Entity 1 polymer

Description description	23S rRNA
Molecular weight formula_weight	941612.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	A
Atom count atom_count	50320

GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCGGUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGAAUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCCAGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGAUACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUGAAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGCGUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUCUUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACUAACUGGAGGACCGAACCGACUAAUGUUGAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGAGAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGGGGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAACGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAAGGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUGCGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUCUGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGGGUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAGGCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAUGUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGUGAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUCGUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAUGGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUACCAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCUCUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACCCGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUGUACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUAUAGCUUGACACUGAACAUUGAGCCUUGAUGUGUAGGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGCCGACCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAACGUUGACCCGUAAUCCGGGUUGCGGACAGUGUCUGGUGGGUAGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGUUAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGGUUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUCGACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCAUUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAGGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGUAGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACCCUUUAAGGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUACUAAUGAACCGUGAGGCUUAACCUU

Entity 2 Entity 2 polymer

Description description	50S ribosomal protein L3
Molecular weight formula_weight	22277.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKKMAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA

Entity 3 Entity 3 polymer

Description description	50S ribosomal protein L4
Molecular weight formula_weight	22121.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLAARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA

Entity 4 Entity 4 polymer

Description description	50S ribosomal protein L13
Molecular weight formula_weight	16050.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI

Entity 5 Entity 5 polymer

Description description	50S ribosomal protein L14
Molecular weight formula_weight	13565.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEVL

Entity 6 Entity 6 polymer

Description description	50S ribosomal protein L17
Molecular weight formula_weight	14393.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDRSEKAEAAAE

Entity 7 Entity 7 polymer

Description description	50S ribosomal protein L20
Molecular weight formula_weight	13528.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA

Entity 8 Entity 8 polymer

Description description	50S ribosomal protein L21
Molecular weight formula_weight	11586.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGISA

Entity 9 Entity 9 polymer

Description description	50S ribosomal protein L22
Molecular weight formula_weight	12253.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S

METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSHITVVVSDR

Entity 10 Entity 10 polymer

Description description	50S ribosomal protein L23
Molecular weight formula_weight	11222.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T

MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE

Entity 11 Entity 11 polymer

Description description	50S ribosomal protein L24
Molecular weight formula_weight	11339.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSETIK

Entity 12 Entity 12 polymer

Description description	50S ribosomal protein L29
Molecular weight formula_weight	7286.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y

MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA

Entity 13 Entity 13 polymer

Description description	50S ribosomal protein L32
Molecular weight formula_weight	6463.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	0

MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK

Entity 14 Entity 14 polymer

Description description	50S ribosomal protein L34
Molecular weight formula_weight	5397.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	2

MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK

Entity 15 Entity 15 polymer

Description description	50S ribosomal protein L30
Molecular weight formula_weight	6554.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Z

MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE

Entity 16 Entity 16 polymer

Description description	50S ribosomal protein L15
Molecular weight formula_weight	15008.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

Entity 17 Entity 17 polymer

Description description	50S ribosomal protein L19
Molecular weight formula_weight	13159.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRERTGKAARIKERLN

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	4.17 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	11975

Cryo-EM captures early ribosome assembly in action

1. Protein Identity and Related Structures Protein Identity & Related Structures

50S ribosomal protein L3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L13

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L14

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L17

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L20

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L21

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L22

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L23

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L24

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer