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8cf4

SH3 domain solved by the exact solid-state method from the Bruker Dynamics Center using the combined correction method with PDB 2NUZ

Method: SOLID-STATE NMR Dmax: 46.0 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Spectrin alpha chain, non-erythrocytic 1

Gallus gallus

UniProt P07751

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 965–1025 Not recorded No other associated polymer SOLID-STATE NMR NMR measurement conditions：pH 7；298 K；Ionic strength (raw mmCIF value) 0；Pressure 1 NMR sample composition：1 na [U-100% 13C; U-100% 15N] SH3 of chicken alpha-spectrin, 100% H2O | 100% H2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8CF4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1AEY ALPHA-SPECTRIN SRC HOMOLOGY 3 DOMAIN, SOLUTION NMR, 15 STRUCTURES Deposited 1997-03-02 Different construct Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SRC HOMOLOGY 3 DOMAIN
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 3.5；297 K
Resolution not provided
1AJ3 SOLUTION STRUCTURE OF THE SPECTRIN REPEAT, NMR, 20 STRUCTURES Deposited 1997-05-14 Different construct Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1763–1872（110 aa） Fragment：16TH REPEAT, RESIDUES 1772 - 1869
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6；308 K
Resolution not provided
1BK2 A-SPECTRIN SH3 DOMAIN D48G MUTANT Deposited 1998-07-14 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 969–1025（57 aa） Fragment：SH3-DOMAIN
Mutation：D48G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；pH 6.0
Resolution 2.01 Å R-free 0.325
1CUN CRYSTAL STRUCTURE OF REPEATS 16 AND 17 OF CHICKEN BRAIN ALPHA SPECTRIN Deposited 1999-08-20 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 1771–1982（212 aa） Fragment：REPEATS 16 AND 17 (RESIDUES 1771 TO 1982 PLUS AN N-TERMINAL MET)
Chain B 1771–1982（212 aa） Fragment：REPEATS 16 AND 17 (RESIDUES 1771 TO 1982 PLUS AN N-TERMINAL MET)
Chain C 1771–1982（212 aa） Fragment：REPEATS 16 AND 17 (RESIDUES 1771 TO 1982 PLUS AN N-TERMINAL MET)
Mutation：L1771V, G1827S Mutation：L1771V, G1827S Mutation：L1771V, G1827S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；1.99M AMMONIUM SULFATE, 0.12M NA, K TARTRATE, 0.1M NA CITRATE PH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.257
1E6G A-SPECTRIN SH3 DOMAIN A11V, V23L, M25I, V53I, V58L MUTANT Deposited 2000-08-15 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 964–1025（62 aa） Fragment：SH3-DOMAIN RESIDUES 964-1025
Mutation：YES SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 90MM SODIUM CITRATE/CITRIC ACID, PH=6.0, 90 MM BIS-TRIS PROPANE, 0.9 MM EDTA, 0.9 MM DTT, 0.9 MM SODIUM AZIDE, pH 6.00
Resolution 2.30 Å R-free 0.271
1E6H A-SPECTRIN SH3 DOMAIN A11V, M25I, V44I, V58L MUTANTS Deposited 2000-08-17 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 964–1025（62 aa） Fragment：SH3-DOMAIN RESIDUES 964-1025
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 90MM SODIUM CITRATE/CITRIC ACID, PH=6.0, 90 MM BIS-TRIS PROPANE, 0.9 MM EDTA, 0.9 MM DTT, 0.9 MM SODIUM AZIDE, pH 6.00
Resolution 2.01 Å R-free 0.276
1E7O A-SPECTRIN SH3 DOMAIN A11V, V23L, M25V, V44I, V58L MUTATIONS Deposited 2000-08-31 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3-DOMAIN, RESIDUES 965-1025
Mutation：YES GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 90MM SODIUM CITRATE/CITRIC ACID, PH=6.0, 90 MM BIS-TRIS PROPANE, 0.9 MM EDTA, 0.9 MM DTT, 0.9 MM SODIUM AZIDE, pH 6.00
Resolution 3.20 Å R-free 0.237
1G2B ALPHA-SPECTRIN SRC HOMOLOGY 3 DOMAIN, CIRCULAR PERMUTANT, CUT AT N47-D48 Deposited 2000-10-18 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1011–1025（15 aa） Fragment：SH3 DOMAIN
Chain A 967–1010（44 aa） Fragment：SH3 DOMAIN
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.12 Å R-free 0.202
1H8K A-SPECTRIN SH3 DOMAIN A11V, V23L, M25V, V53I, V58L MUTANT Deposited 2001-02-09 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3-DOMAIN RESIDUES 965-1025
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 0.1M ACETATE/AC. ACETIC, PH=4.5, 90 MM BIS-TRIS PROPANE, 0.9 MM SODIUM AZIDE, pH 4.50
Resolution 2.70 Å R-free 0.221
1HD3 A-SPECTRIN SH3 DOMAIN F52Y MUTANT Deposited 2000-11-06 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 964–1025（62 aa） Fragment：SH3-DOMAIN RESIDUES 964-1025
Mutation：YES SO4 SULFATE ION × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 90MM SODIUM CITRATE/CITRIC ACID, PH=6.0, 90 MM BIS-TRIS PROPANE, 0.9 MM EDTA, 0.9 MM DTT, 0.9 MM SODIUM AZIDE, pH 6.00
Resolution 1.98 Å R-free 0.265
1M8M SOLID-STATE MAS NMR STRUCTURE OF THE A-SPECTRIN SH3 DOMAIN Deposited 2002-07-25 Different construct Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SRC HOMOLOGY 3 DOMAIN (RESIDUES 965-1025)
Not recorded No recorded non-water small molecule SOLID-STATE NMR
NMR measurement conditions pH 7.5；280 K；Ionic strength (raw mmCIF value) SOLID SAMPLE；Pressure AMBIENT
NMR sample composition A 200 MM (NH4)2SO4 SOLUTION WAS ADDED TO A 1.15 MM SH3 SOLUTION (PH=3.5) AT A VOLUME RATIO 1:1. THE SOLID SAMPLE WAS OBTAINED BY PRECIPITATION, CHANGING THE PH TO 7.5 IN NH3 ATMOSPHERE.
Resolution not provided
1NEG Crystal Structure Analysis of N-and C-terminal labeled SH3-domain of alpha-Chicken Spectrin Deposited 2002-12-11 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1024（60 aa） Fragment：SH3-domain
Not recorded AZI AZIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；Dioxane, MES, Ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.249
1PWT THERMODYNAMIC ANALYSIS OF ALPHA-SPECTRIN SH3 AND TWO OF ITS CIRCULAR PERMUTANTS WITH DIFFERENT LOOP LENGTHS: DISCERNING THE REASONS FOR RAPID FOLDING IN PROTEINS Deposited 1998-10-06 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 967–1025（59 aa） Fragment：SH3 DOMAIN
Mutation：MGTG INSTEAD OF MDETG AT THE N-TERMINUS No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4；pH 4
Resolution 1.77 Å R-free 0.246
1QKW Alpha-spectrin Src Homology 3 domain, N47G mutant in the distal loop. Deposited 1999-08-16 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 964–1025（62 aa） Fragment：SH3 DOMAIN
Mutation：YES SO4 SULFATE ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 90MM BIS-TRIS PROPANE, 0.9MM EDTA AND 0.9MM DTT., pH 6.00
Resolution 2.00 Å R-free 0.243
1QKX Alpha-spectrin Src Homology 3 domain, N47A mutant in the distal loop. Deposited 1999-08-16 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 964–1025（62 aa） Fragment：SH3 DOMAIN RESIDUES 964-1025
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；DROP CONTAINS 1.1 M AMM.SULPH., 90MM CITRIC BUFFERPH=6.0, 90MM BIS-TRIS PROPANE, 0.9MM EDTA AND 0.9MM DTT., pH 6.00
Resolution 1.80 Å R-free 0.275
1SHG CRYSTAL STRUCTURE OF A SRC-HOMOLOGY 3 (SH3) DOMAIN Deposited 1993-05-19 Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å R-free 0.277
1TUC ALPHA-SPECTRIN SRC HOMOLOGY 3 DOMAIN, CIRCULAR PERMUTANT, CUT AT S19-P20 Deposited 1996-02-29 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 983–1061（79 aa） Fragment：SRC HOMOLOGY 3 DOMAIN
Mutation：CIRCULAR PERMUTANT, CUT AT S19-P20, INS(MG-P20), INS(SG-T4) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.02 Å R-free 0.290
1TUD ALPHA-SPECTRIN SRC HOMOLOGY 3 DOMAIN, CIRCULAR PERMUTANT, CUT AT N47-D48 Deposited 1996-02-29 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 952–1010（59 aa） Fragment：SRC HOMOLOGY 3 DOMAIN
Mutation：CIRCULAR PERMUTANT, CUT AT N47-D48, INS(M-D48), INS(SG-T4) No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.77 Å R-free 0.248
1U06 crystal structure of chicken alpha-spectrin SH3 domain Deposited 2004-07-13 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain
Not recorded AZI AZIDE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.49 Å R-free 0.189
1U4Q Crystal Structure of Repeats 15, 16 and 17 of Chicken Brain Alpha Spectrin Deposited 2004-07-26 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1662–1982（321 aa） Fragment：repeats 15, 16, 17 (residues 1662 to 1982)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；283 K；24% PEG 3.3K, 0.2M Lithium Sulfate, 2% MPD, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 283, 287K
Resolution 2.50 Å R-free 0.313
1U4Q Crystal Structure of Repeats 15, 16 and 17 of Chicken Brain Alpha Spectrin Deposited 2004-07-26 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1662–1982（321 aa） Fragment：repeats 15, 16, 17 (residues 1662 to 1982)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；283 K；24% PEG 3.3K, 0.2M Lithium Sulfate, 2% MPD, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 283, 287K
Resolution 2.50 Å R-free 0.313
1U5P Crystal Structure of Repeats 15 and 16 of Chicken Brain Alpha Spectrin Deposited 2004-07-28 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1662–1876（215 aa） Fragment：repeats 15 and 16 (residues 1662 to 1876)
Not recorded K POTASSIUM ION × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；287 K；1M NaH2PO4, 1M K2HPO4, 0.2M ammonium acetate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K
Resolution 2.00 Å R-free 0.225
1UUE a-SPECTRIN SH3 DOMAIN (V44T, D48G MUTANT) Deposited 2003-12-18 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 DOMAIN, RESIDUES 965-1025
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；pH 7.00
Resolution 2.60 Å R-free 0.266
2CDT alpha-SPECTRIN SH3 DOMAIN A56S MUTANT Deposited 2006-01-27 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3-DOMAIN, RESIDUES 969-1025
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；1.1M AMMONIUM SULPHATE, 90MM SODIUM CITRATE/CITRIC ACID, PH 6.0, 90 MM BIS-TRIS PROPANE, 0.9MM EDTA, 0.9MM DTT, 0.9MM SODIUM AZIDE
Resolution 2.54 Å R-free 0.288
2F2V alpha-spectrin SH3 domain A56G mutant Deposited 2005-11-18 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：Src-homology 3 domain, SH3 domain
Mutation：A56G FMT FORMIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；288 K；2M Sodium Formiate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 288K
Resolution 1.85 Å R-free 0.223
2F2W alpha-spectrin SH3 domain R21A mutant Deposited 2005-11-18 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：Src-homology 3 domain
Mutation：R21A SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 3；298 K；Ammonium sulphate 2M, pH 3, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.246
2F2X alpha-spectrin SH3 domain R21G mutant Deposited 2005-11-18 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：Src-homology 3 domain
Mutation：R21G SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 3；298 K；Ammonium sulphate 2M, pH 3, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.60 Å R-free 0.235
2JM8 R21A Spc-SH3 free Deposited 2006-10-25 Different construct Different mutation/modification Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain, residues 965-1025
Mutation：R21A No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 3.5；300 K
NMR sample composition 2 mM R21A Spc-SH3 free, 90 % H2O, 10 % D2O, 20 mM d5-glycine | 90% H2O/10% D2O
Resolution not provided
2JM9 R21A Spc-SH3 bound Deposited 2006-10-25 Different construct Different mutation/modification Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain, residues 965-1025
Mutation：R21A No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 3.5；300 K
NMR sample composition 2 mM R21A Spc-SH3 bound, 5.2 mM P41, 90 % H2O, 10 % D2O, 20 mM d5-glycine | 90% H2O/10% D2O
Resolution not provided
2JMA R21A Spc-SH3:P41 complex Deposited 2006-10-25 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 965–1025（61 aa） Fragment：SH3 domain, residues 965-1025
Mutation：R21A No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 3.5；300 K
NMR sample composition 2 mM R21A Spc-SH3, 5.2 mM P41, 90 % H2O, 10 % D2O, 20 mM d5-glycine | 90% H2O/10% D2O
Resolution not provided
2JMC Chimer between Spc-SH3 and P41 Deposited 2006-11-02 Different construct Different mutation/modification Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 983–1025（43 aa）
Chain A 967–980（14 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 3.5；303.15 K；Pressure 1
NMR sample composition 1 mM [U-98% 13C; U-98% 15N] SPCp41, 20 mM glycine, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-98% 15N] SPCp41, 20 mM glycine, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM SPCp41, 20 mM glycine, 90% H2O, 10% D2O | 90% H2O/10% D2O
Resolution not provided
2KR3 Solution structure of SHA-D Deposited 2009-12-03 Different construct Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：alpha spectrin SH3 domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 3.5；298 K；Ionic strength (raw mmCIF value) 20；Pressure AMBIENT
NMR sample composition 1.5mM [U-98% 13C; U-98% 15N] SHA-D; 20mM [U-99% 2H] sodium acetate; 0.03% sodium azide; 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2KXD The structure of SH3-F2 Deposited 2010-04-30 Different construct Different mutation/modification Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 984–1025（42 aa）
Chain A 967–982（16 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 4；298 K；Ionic strength (raw mmCIF value) 20；Pressure AMBIENT
NMR sample composition 1.5mM [U-98% 13C; U-98% 15N] SH3-F2-1, 20mM [U-99% 2H] sodium acetate-2, 0.03 % sodium azide-3, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2LJ3 PFBD: High-throughput Strategy of Backbone fold Determination for small well-folded proteins in less than a day Deposited 2011-09-04 Different construct Different mutation/modification Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 Domain (UNP RESIDUES 965-1025)
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition 1.00 mM [U-99% 13C; U-99% 15N] sodium phosphate-1, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2NUZ crystal structure of alpha spectrin SH3 domain measured at room temperature Deposited 2006-11-10 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；200mM ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.85 Å R-free 0.184
2OAW Structure of SHH variant of "Bergerac" chimera of spectrin SH3 Deposited 2006-12-18 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 969–1025（57 aa） Fragment：SH3 DOMAIN
Not recorded CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；295 K；7,5% Na malonate, 20mM AcNa, 100 mM glycine, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.90 Å R-free 0.264
2OAW Structure of SHH variant of "Bergerac" chimera of spectrin SH3 Deposited 2006-12-18 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 969–1025（57 aa） Fragment：SH3 DOMAIN
Not recorded CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；295 K；7,5% Na malonate, 20mM AcNa, 100 mM glycine, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.90 Å R-free 0.264
2OAW Structure of SHH variant of "Bergerac" chimera of spectrin SH3 Deposited 2006-12-18 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 969–1025（57 aa） Fragment：SH3 DOMAIN
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；295 K；7,5% Na malonate, 20mM AcNa, 100 mM glycine, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.90 Å R-free 0.264
2OAW Structure of SHH variant of "Bergerac" chimera of spectrin SH3 Deposited 2006-12-18 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 969–1025（57 aa） Fragment：SH3 DOMAIN
Not recorded CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；295 K；7,5% Na malonate, 20mM AcNa, 100 mM glycine, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.90 Å R-free 0.264
2OAW Structure of SHH variant of "Bergerac" chimera of spectrin SH3 Deposited 2006-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 969–1025（57 aa） Fragment：SH3 DOMAIN
Chain B 969–1025（57 aa） Fragment：SH3 DOMAIN
Chain C 969–1025（57 aa） Fragment：SH3 DOMAIN
Chain D 969–1025（57 aa） Fragment：SH3 DOMAIN
Not recorded CL CHLORIDE ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；295 K；7,5% Na malonate, 20mM AcNa, 100 mM glycine, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.90 Å R-free 0.264
2RMO Solution structure of alpha-spectrin_SH3-bergerac from Chicken Deposited 2007-11-07 Different construct Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：Alpha-Spectrin Sh3 Domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 4；298 K；Ionic strength (raw mmCIF value) 25；Pressure 74
NMR sample composition 2.5mM [U-98% 13C; U-98% 15N] alpha-spectrin SH3-bergerac; 90% H2O, 10% [U-99% 2H] D2O; 25mM [U-99% 2H] sodium acetate; 0.03% sodium azide; 90% H2O, 10% D2O | 90% H2O/10% D2O
Resolution not provided
2ROT Structure of chimeric variant of SH3 domain- SHH Deposited 2008-04-10 Different construct Different experimental method Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：ALPHA-SPECTRIN SH3 Domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 3.5；299 K；Ionic strength (raw mmCIF value) 20；Pressure 74
NMR sample composition 2.5mM [U-98% 13C; U-98% 15N] alpha-spectrin SHH, 90% H2O, 10% [U-99% 2H] D2O, 25mM [U-99% 2H] sodium acetate, 0.03% sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
3I9Q Crystal Structure of the triple mutant S19G-P20D-R21S of alpha spectrin SH3 domain Deposited 2009-07-12 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 969–1025（57 aa） Fragment：SH3 domain (UNP residues 969 to 1025)
Mutation：S19G, P20D, R21S SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；2 M ammonium sulphate, 0.1 M MES pH 6.5, vapor diffusion, hanging drop, temperature 298K
Resolution 1.45 Å R-free 0.243
3M0P Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Crystal obtained in ammonium sulphate at pH 4. Deposited 2010-03-03 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：UNP residues 965 to 1025
Mutation：R21D SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；0.8 M ammonium sulphate, 0.1 M sodium acetate pH 4, vapor diffusion, hanging drop, temperature 298K
Resolution 2.60 Å R-free 0.281
3M0Q Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Crystal obtained in ammonium sulphate at pH 5. Deposited 2010-03-03 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：UNP residues 965 to 1025
Mutation：R21D SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions COUNTER-DIFFUSION；pH 5；298 K；0.8 M ammonium sulphate, 0.1 M sodium acetate pH 5, counter diffusion, temperature 298K
Resolution 1.75 Å R-free 0.228
3M0R Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Crystal obtained in ammonium sulphate at pH 6. Deposited 2010-03-03 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain (UNP residues 965 to 1025)
Mutation：R21D SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；0.8 M ammonium sulphate, 0.1 M Bis-Tris pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.10 Å R-free 0.192
3M0S Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Crystal obtained in ammonium sulphate at pH 7 Deposited 2010-03-03 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 969–1025（57 aa） Fragment：UNP residues 965 to 1025
Mutation：R21D SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions COUNTER-DIFFUSION；pH 7；298 K；0.8 M ammonium sulphate, 0.1 M HEPES pH 7, counter diffusion, temperature 298K
Resolution 1.60 Å R-free 0.209
3M0T Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Crystal obtained in ammonium sulphate at pH 9. Deposited 2010-03-03 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：UNP residues 965 to 1025
Mutation：R21D SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions COUNTER-DIFFUSION；pH 9；298 K；0.8 M ammonium sulphate, 0.1 M Bicine pH 9, counter diffusion, temperature 298K
Resolution 1.70 Å R-free 0.215
3M0U Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5. Deposited 2010-03-03 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：UNP residues 965 to 1025
Mutation：R21D FMT FORMIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions batch, under oil；pH 6.5；298 K；2 M sodium formate, 0.1 M MES pH 6.5, batch, under oil, temperature 298K
Resolution 1.10 Å R-free 0.168
3NGP High resolution structure of alpha-spectrin SH3 domain mutant with a redesigned core Deposited 2010-06-12 Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain (UNP residues 965 to 1025)
Mutation：A11V, V23L, M25V, V44I,V58L,D48G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；2M ammonium sulphate, 0.1 M MES , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.08 Å R-free 0.185
4F16 Crystal Structure of the alpha spectrin SH3 domain at pH 5 Deposited 2012-05-06 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain, UNP residues 965-1025
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 5；298 K；0.1M Sodium Acetate, 30% PEG 8000, pH 5, capillary counterdiffusion, temperature 298K
Resolution 1.93 Å R-free 0.254
4F17 Crystal Structure of the alpha spectrin SH3 domain at pH 9 Deposited 2012-05-06 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain, UNP residues 965-1025
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions capillary counterdiffusion；pH 9；298 K；0.1M Tris-HCl, 20% PEG 400, 15% PEG 4000, 10% PEG 8000, pH 9, capillary counterdiffusion, temperature 298K
Resolution 1.55 Å R-free 0.217
5IHI Crystal Structure of the alpha spectrin SH3 domain double mutant V46G-D48G Deposited 2016-02-29 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain
Not recorded SO4 SULFATE ION × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；0.1M Sodium Acetate, 1 M Ammonium sulphate
Resolution 1.45 Å R-free 0.193
5IHK Crystal Structure of the alpha spectrin SH3 domain mutant N47A Deposited 2016-02-29 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain
Not recorded SO4 SULFATE ION × 1 PRO PROLINE × 1 ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；0.1M Sodium Acetate, 1 M Ammonium sulphate, 1 M Proline
Resolution 1.35 Å R-free 0.192
5IHN Crystal Structure of the alpha spectrin SH3 domain mutant N47G Deposited 2016-02-29 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa） Fragment：SH3 domain
Mutation：N47G FMT FORMIC ACID × 1 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；2M Sodium Formate, 0.1 M MES
Resolution 1.50 Å R-free 0.178
7S4R Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - alpha Spectrin-SH3 domain Deposited 2021-09-09 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 970–1025（56 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；277 K；Crystals were obtained by applying several concentration/dilution steps with milli-Q water inside standard Amicon Ultra 0.5 ml centrifugal filter (3 kDa cutoff). A crystalline slurry appeared when most citric acid buffer was removed
Resolution 2.10 Å R-free 0.240
8CHG SH3 domain solved by the exact solid-state method from the Bruker Dynamics Center using the correction for dipolar truncation with PDB 2NUZ Deposited 2023-02-08 Parsed fields agree Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa）
Not recorded No recorded non-water small molecule SOLID-STATE NMR
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 0；Pressure 1
NMR sample composition 1 na [U-100% 13C; U-100% 15N] SH3 of chicken alpha-spectrin, 100% H2O | 100% H2O
Resolution not provided
8CHH SH3 domain solved by the exact solid-state method from the Bruker Dynamics Center using the correction method for spin-diffusion with PDB 2NUZ as reference Deposited 2023-02-08 Parsed fields agree Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa）
Not recorded No recorded non-water small molecule SOLID-STATE NMR
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 0；Pressure 1
NMR sample composition 1 na [U-100% 13C; U-100% 15N] SH3 of chicken alpha-spectrin, 100% H2O | 100% H2O
Resolution not provided
9EYB A-SPECTRIN SH3 DOMAIN V9L, A11V, V23L, M25C, V46W, V53L and V58L MUTANT Deposited 2024-04-09 Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain AAA 965–1025（61 aa）
Mutation：V9L, A11V, V23L, M25C, V46W, V53L, V58L GOL GLYCEROL × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.8M Ammonium Sulfate and 0.1M HEPES pH: 7.0
Resolution 1.34 Å R-free 0.175
9EYC A-SPECTRIN SH3 DOMAIN V9L, A11V, V23L, M25C, V46W, V53L and V58L MUTANT Deposited 2024-04-09 Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa）
Mutation：V9L, A11V, V23L, M25V, V46W, V53L, V58L GOL GLYCEROL × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；1.8M Ammonium Sulfate, 0.05 NaI and 0.1M TRIS pH: 6.5
Resolution 1.06 Å R-free 0.216
9EYE A-SPECTRIN SH3 DOMAIN V9L, A11V, L31V, V44I, V53I MUTANT Deposited 2024-04-09 Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain AAA 965–1025（61 aa）
Mutation：V9L, A11V, L31V, V44I, V53I GOL GLYCEROL × 1 PO4 PHOSPHATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0M Ammonium Sulfate and 0.1M TRIS pH: 6.5
Resolution 1.13 Å R-free 0.169
9EYF A-SPECTRIN SH3 DOMAIN V9L, A11V, V23L, M25L, V46Y, V53I MUTANT Deposited 2024-04-09 Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa）
Mutation：V9L, A11V, V23L, M25L, V46Y, V53I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；2.2M Ammonium Sulfate and 0.1M TRIS pH: 6.7
Resolution 1.34 Å R-free 0.252
9IH6 A-SPECTRIN SH3 DOMAIN V9Y, A11V, V23L, M25V, V53L, V58L MUTANT Deposited 2025-02-20 Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 965–1025（61 aa）
Mutation：V9Y, A11V, V23L, M25V, V53L, V58L GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2M Ammonium Sulfate, 1M Na/K Tartrate and 0.1M Sodium Citrate
Resolution 1.31 Å R-free 0.225
9IH7 A-SPECTRIN SH3 DOMAIN V9L, A11V, V23I, M25V, V46F, V53L, V58L MUTANT Deposited 2025-02-20 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 967–1025（59 aa）
Mutation：V9L, A11V, V23I, M25V, V46F, V53L, V58L SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2M Ammonium Sulfate and 0.1M Bis-Tris
Resolution 1.12 Å R-free 0.219
9IHA A-SPECTRIN SH3 DOMAIN V9L, A11V, M25V, V46F, V58L MUTANT Deposited 2025-02-20 Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain AAA 965–1025（61 aa）
Mutation：V9L, A11V, M25V, V46F, V58L SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2M Ammonium Sulfate and 0.1M Bis-Tris
Resolution 1.01 Å R-free 0.174

60 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SPTN1_CHICK
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–62； UniProt 965–1025

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8cf4

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8cf4
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8cf4
Deposition date deposition_date2023-02-02
Structure title titleSH3 domain solved by the exact solid-state method from the Bruker Dynamics Center using the combined correction method with PDB 2NUZ
Keywords keywordssolid-state NMR spectroscopy, structure elucidation, integrated structural biology, protein dynamics, PEPTIDE BINDING PROTEIN; PEPTIDE BINDING PROTEIN
Experimental Method methodSOLID-STATE NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier11.92 Å
Radius of gyration Rg (electron density) rg_electron11.49 Å
Forward intensity I(0) i071773000.00
Molecular weight molecular_weight72183.0 kDa
Excluded volume excluded_volume91185 ų
Envelope volume envelope_volume17800 ų
Hydration-shell volume shell_volume11180 ų
Envelope diameter envelope_diameter49.6 Å
Shell Rg shell_rg19.55 Å
Envelope Rg envelope_rg14.67 Å
Shape Rg shape_rg11.41 Å
Total Rg total_rg12.15 Å
Total atoms total_atoms10200
Residues n_residues620
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax46.0 Å
Rg (real space) rg_real11.94 Å
Rg uncertainty (real space) rg_real_error0.40 Å
I(0) (real space) i0_real7.1770e+07
I(0) uncertainty (real space) i0_real_error8.1040e+05
Rg (reciprocal space) rg_reciprocal11.94 Å
I(0) (reciprocal space) i0_reciprocal71770000.0000
Solution quality estimate total_estimate0.7668
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary15.2 Å
Skewness Skewness skewness0.463
Kurtosis Kurtosis kurtosis0.319
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha143100.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.364; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.883; Smooth: 0.990
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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8. Citations (1)

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9. Files and Curves (10)