8hkv

Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome

Method: ELECTRON MICROSCOPY D_max: 270.6 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8hkv
沉积日期 deposition_date	2022-11-28
结构标题 title	Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome
关键词 keywords	Sulfolobus acidocaldarius ribosome small subunit, RIBOSOME; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	73.36 Å
回转半径 Rg (电子) rg_electron	74.46 Å
零角强度 I(0) i0	71935700000.00
分子量 molecular_weight	1520400.0 kDa
排除体积 excluded_volume	1586900 Å³
包络体积 envelope_volume	2643000 Å³
水化壳体积 shell_volume	272470 Å³
包络直径 envelope_diameter	243.0 Å
壳层 Rg shell_rg	86.59 Å
包络 Rg envelope_rg	74.05 Å
形状 Rg shape_rg	74.51 Å
总 Rg total_rg	74.44 Å
总原子数 total_atoms	102540
残基数 n_residues	7585
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	270.6 Å
Rg (实空间) rg_real	76.42 Å
Rg 误差 (实空间) rg_real_error	1.49 Å
I(0) (实空间) i0_real	7.1950e+10
I(0) 误差 (实空间) i0_real_error	1.4180e+09
Rg (倒空间) rg_reciprocal	74.10 Å
I(0) (倒空间) i0_reciprocal	72070000000.0000
解质量估计 total_estimate	0.8869
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	90.2 Å
偏度 Skewness skewness	0.524
峰度 Kurtosis kurtosis	0.340
角度范围 angular_range	— – 0.1050 Å⁻¹
当前正则化参数 α current_alpha	0.9727
最高正则化参数 α highest_alpha	5883000000.0000
实空间数据点数 n_real_points	22
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.678; Stabil: 0.890; Sysdev: 1.000; Positv: 1.000; Valcen: 0.947; Smooth: 0.894

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (37)

Entity 1 polymer

描述 description	23s rRNA (2996-MER)
分子量 formula_weight	980552.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	A23S
原子数 atom_count	102540

GCCGAAGCCUCCCGGUGGAUGGCUCGGCUCGGGCACCGAAGAAGGGCGCGGCAAGCAGCGAAAUGCUCGGGUGAGGCGCAAGCAGCCGUUGACCCCGAGGUCCCCUAAUGGGAUAUCCUGCCGGGUUUCCGGCGCUCCCGGUUUAUAACUGGGAGUGGGAACCCCCCGAACGGAAACAUCUUAGUAGGGGGAGGAAAAGAAAUCAAUUGAGAUCCCCUGAGUAGGGGCGACCGAAAGGGGGACAGCCCAAACUAAACCUGCCGAUGAUAAGUCGGUGGGGAUGUGGUGUUACGACCUCUAGCCUGAGGUUCGACCUCGGCUUUCCUAACCUAUCUAGCCGAACUCCCCUGGAACGGGGGGCCAUAGAGGGUGAAAGCCCCGUAGGCUAAAGAUAGGUGGAAAGUGGCUAGAGGUAGAGUACCAUCCCCUGGUUUGGGGGUGGGAAGUUAGGGGACACGUGCCUCUAAGGCUAAAUAUGUCCCGAGACCGAUAGCAAACUAAGUACCGUGAGGGAAAGCUGAAAAGAACCCCGGAAGGGGGAGUGCAAAGAGCCUGAAACCGGGUGGUUAUACAGGGUGUGGCUCGAAAGAAGUGAACCCUUCCGAAGGAAAGGGGCGCAAGCCCUUAGUACGAGGAAGGGCGAUCGGGGUCACGCCUUUCGUCUUGAAACACGGGCCGGGGAGUUCACAUCAGUGGCGAGCUUAAGGAGAUUAUCUCCGAAGGCAUAGGGAAACCAAGUGCCCGCAGCCUAGUUUCUAGGCGAGGGGCAGGGUCUGUCAGGGCCUGAAGCCACUGAUGUGAGGCUAGAAACCGGGCGAUCUAGUCCGGGGCAGGCUGAAGGUGGGGGAAACCCCACUGGAGGGCCGAAUAGGGGUUCUGACGUGCAAUUCGUUCCCUUGACCUCGGACUAGGGGCAAAAGACCAAUCUAGCCCGGUGAUAGCUAGUUCCCCCCGAAAUGCGUCCUAGCGCAGCCUCCCUAAAGGCAGCUCGCGGGGUAGAGUGACAGAUCGGGGGCUCCAGGCGAAAGCCUGGGGCUUCCGGUCUAACUCCGAACCCACGAGCGCCGAAGAAGGGGGGAGUGGGUCACUCGGCGUAAGGUUGGGUGGCAAAAGGGGAACAACCCAGACCUGGGUUAAGGCCCCAAAGUCCCGGCUAAGUGCCAACGAAAAGGCGUCUCCAGCCUUAGACAGCGGGAAGGUGGGCCCAGCAGCAGCCAUCCUCUAAGGAGUGCGUAACAGCUCACCCGCCGAGGCUGGAGGCCCUAAAGAUUGGUCGGGGCUCAAGCCGGGCGCCGAGACCCAGGAGGGGGUCUCUACUAGAGAUCCUCUGGUAGGGGGGCGCUGUGAUGGGGUAGAAGGUGGGUCGUGAGAUCCACUGGACCCGUCACAGGUGCAGAUCCCGGCGGUAGUAACAGCGAAGGGGGGUGAGAAUCCCCCUCGCCGGAAGGGCAAGGGUUUCCCGGCAACGUUCGUCAGCCGGGAGUUAGCCGGUCCUAAGGUAGGGCCUAAUAGGUACCUACCGAAAGGGAAAGGGGUUAACAUUCCCCUGCCUCCCGGGUAGGUGCGGUAACGCAAGCCAGACUCCUGACGGAUUGGGGUAGGGAGAGUAGGACCACCGUCCUACCCAAGCACUCAAGCCCUUGGAGAGCCGUAACGGUGAGAAGAGGGCGAAGGUGUGAUGGGCCUUCCGUUAGGAGGGUUCUCCUGAUCCCUAGUCCCCAUGAAAAGGGAGUCUGGAACGAUCCCGGGAGACCGUACCUAGAACCGACACUGGUGCCCCUGGGUGAGAAGCCCAAGGCGUCUGAGGGGUAACCCAGGCUAGGGAACUCGGCAAAUUAGCCCCGUAACUUCGGGAGAAGGGGUGCCUAUCGUGGUUUAACAAAGCCACGAUAGGUCGCAGUGACCAGAGGGACCUGACUGUUUAAUAAAAACAUAGGUCCCCGCUAGCCCGAAAGGGUGUGUACGGGGGCUAAAUCCUGGCCACUGGUGGUUGGUUAAAUCCGGGUUCAACCGGGCGAAGCCCCACCGAAGGCCGGGGGUAACUCUGACCCUCUUAAGGUAGCCAAAUGCCUUGCCGGGUAAGUUCCGGCGCGCAUGAAUGGAUCAACGCGGUCCCUACUGUCCCAGCCUGGGGCCUCGUGAACGCCCUGAGCCGGUGCACAGUCCGGCAUCUCCCUACACCGAGAGAAGACCCCGUGGAGCUUCACCGCAGCCUGGCGUUGUCCCUCGGGCGUUUAUGCGUAGAGUAGGUGGGAGGGGUCGAACCUGUCCUUUCGGGGGCAGGGGACCCGAAAGUGAAACACCACCCAUGGACGCUCGAGGGACUAACCUCUCGAAAGAGAGGAACAACGUCAGGUGGGCGGUUCGGCUGGGGCGGCACUCCCGCGAAAAGAUAACACGGGAGCCCAAAGGUCGGCUCAGGCGGUACAGAACGCCGCCGUAGAGCGCAAGGGCAAAAGCCGGCCUGACGUGACCCUUCCAAGUACGGGGUCACGACGCGAAAGCGGGGCCUAGCGAACGCUCGUGCCCCCACACGUGGGGGCCGGGCAUGACAGAAAAGUUACCCCGGGGAUAACAGGGUCGUCGCGGGCGAGAGCUCACAUCGACCCCGCGGUUUGCUACAUCGAUGUCGGCUCUUCCCACCCUGGAGGUGCAGCUGCCUCCAAGGGUAGGGCUGCCCGCCCGUUAAAGGGGAGCGUGAGCUGGGUUUAGACCGUCGCGAGACAGGUCGGACUCUAAGGGUAGGGAGUGCGGACCGCUUGAGGGGAAGGAACCCCUAGUACGAGAGGAACAGGGUUCCGGGGCCUCCAGUUUACCGGUUGUCCGGUAGGGCAAUGCCGGGCAGCCGCGCCCUGAGGGGUAACCGCUGAAAGCAUCUAAGCGGGAACCCCUCCCCUAAAAGAGGCGGUCAGGCGUUAGCCGGGGCCUUCCCCUAGAAGAGGGGGUUGAUGGGGUGGGGAUGUAAGCUCCAAGGUUGUAAGACCGAGGAGUUUAGUCCGCCACUCCCAAUCAGGCUCCCCCC

Entity 2 polymer

描述 description	5s rRNA (122-MER)
分子量 formula_weight	39440.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	A5S

UGCCCACCCGGUCAUAGUGAGCGGGUAACACCCGGACUCGUUUCGAACCCGGAAGUUAAGCCGCUCACGUCAGAGGGGCCGUGGGAUCCGAGAGGGCCCGCAGCCUCUCUGAGCUGGGAUGG

Entity 3 polymer

描述 description	50S ribosomal protein L2
分子量 formula_weight	24881.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AL2P

GKKLLQQRAGRGGINFRSPSWRRVGPARYPNIEGDHKGKIIDILHNPGVTAPVVKVKLDNGLQFYIPAVQGVAVGQEISIGKNATISNGNIVEVGQLPEGTVICNVEKLKGDGGKFARAAGSYAVISGKAGNKVLIKLSSEKIVEVSQNARATVGIIAGGGFVEKPLLKAGNNYWKYRVRAVKWPVVRGVAMNAVSHPHGGGLHQSVSRPSTVSRNAPPGRKVGHIASRRTGRR

Entity 4 polymer

描述 description	50S ribosomal protein L3
分子量 formula_weight	38186.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AL3P

RKLSSPRRGSAGLRPRKRADEILPTPKNWPLVNLKEPKLLGFIGYKAGMTHVYMIDDKPTSPNYGKEVYTPVTIVESPPILGLALRAYHIDSKGELSVLVDYWANFEEGSLKYLKRKITSLKVDSSKMKEKLDLIQKNLNNITYMRLLVSTQPWLVPSLGKKRPEIVEIQIGGGSIQDQLNYGLSLLGKQIPVRDVFREGQLTDIIGVTKGKGFQGVIKRYSVVEFPRWHKHRKGSRKIGARGPSISTPSYVPQPGQLGFHRRTEYNKRIIKIGDNVNEINPAGGIVNYGLVKNTYLVIEGSVLGSRKRPLFLRYPIRPSWSPESAPKITYVNLASQQG

Entity 5 polymer

描述 description	50S ribosomal protein L4
分子量 formula_weight	27358.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AL4P

KVSVLDLKGNQLEEIELPLFFSYPVRKDLIRRVFLSEFTKSLQPKGRDPLAGKRTSALSFGINLGIARVPRVKGSGEAALAPNTVGGRLAFPPTTEKRLVEEVNLKEKKLGIISALAATADPNFVKARGHRFTSNNVPIILVDDFENISKAKEIMDILKSIGVVDDIKRVKESKGVRAGKGKMRGRRYQIAKGPLIVVSNHKSPVVESASNIPGVNVVSANLVSVIHLAPGGHPGRLTIYTKSSINILRQR

Entity 6 polymer

描述 description	50S ribosomal protein L5
分子量 formula_weight	19095.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AL5P

KENVMRRVVLDKVTVNIGVGESGERLQKAYQLVQELTGVKPVYTKGRKSIREFGVRKGAPIGVKATLRRQAAVEFLKKVLPAVNFRLKQSSFDNYGNVSFGIAEHVLIPGTRYDPEIGIFGMDVAITLVRPGYRTMKRKRKKASIPRRHRVTKEEAINFMKENFNVTI

Entity 7 polymer

描述 description	50S ribosomal protein L6
分子量 formula_weight	20305.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AL6P

LKAAYIREEIQIPDKVKVSLENNVLKVKGPKGEVIKDFSYAKGIRIQLNEGKIILETTFADRRKKALLYSIIAHIKNMITGTINGYRYYLKVISTHFPISVKVSGDEVQVSNLIGEKNIRRAKILPGVKVTVKGEDIVVEGSDIYNVAQTAANIESSTKIVGYDRRIFSDGIYIYKKEVIG

Entity 8 polymer

描述 description	50S ribosomal protein L18Ae
分子量 formula_weight	8926.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	ALX0

VKIFMVRGTAIFSASRFPTSQKFTKYVRALNEKQAIEYIYSQLGGKNKIKRYNIHIQEIKEVKEDEITDKTIRDLA

Entity 9 polymer

描述 description	50S ribosomal protein L10e
分子量 formula_weight	18670.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L10E

RPGRCYRKFSGPAYTRREYIPGIPMPKITKFVMGNVNGDYDYELRLITTEKGQIRHNALEAARVIALKYLSKKLASEQNFALVVTKYPHHVIRENKMMAFAGADRLQDGMRLSFGKPIGTAARLEKLGELVMYVRVKKEHLEAAKEALKIASSKLPIRTKIEIS

Entity 10 polymer

描述 description	50S ribosomal protein L13
分子量 formula_weight	15808.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L13P

VIVDATNQILGRMSTHIAKLLKSGKKVYVVNAEKAIISGPRSRVLEGYSLLFTVKTMQNPYRQGIRRPRNPINIVKRTVRGMLPKNKLGKQIYRNLKAYIGIPKELEGKSMIRFDDADVSRLKGKYITVAELSRLLGGLK

Entity 11 polymer

描述 description	50S ribosomal protein L14e
分子量 formula_weight	9559.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L141,L142

AIEIGRICVKTRGREAGKKCVIVDIIDSNFVLVTGPKEINGVKRRRVNILHIEPTDKKVEINKGATDQEVKQAIEQSGLTDFIKEA

Entity 12 polymer

描述 description	50S ribosomal protein L14
分子量 formula_weight	14707.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L14P

MNVLGSRKGFTPALQHNSSVVVADNSGAKEAVIIGIYGYRGVLRRVPFANIADLVMVSVRKGSPDVRKQKFKAVIVRQRMPFRRPDGTWISFEDNAVVIINPDGTPKGTEVRGPIAREAAERWPKIASVATMVI

Entity 13 polymer

描述 description	50S ribosomal protein L15e
分子量 formula_weight	20179.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L15E

SMYHYIEQTWQNKDWRRSILRKRLIEWRRQPAITRIDRPTRLNRARALGYKAKQGFVMVRVRVRRGGLNKPRPNSGRRPKRMGVYGYGPSKGYRWIAEERAARKFPNLEVLGSYYVAEDGMYKYYEVIMVDYSHPVIRSDPELKWLQDSVNRKRVFRGLTSAGQKSRGL

Entity 14 polymer

描述 description	50S ribosomal protein L18e
分子量 formula_weight	12747.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L18E

TNIMLRKLITSLKKQDKAIWVRVAEELEAPRRKRAYINIYKINRYSKANDIIVVPGKVLGVGNLDHPVTVVALSFSKPAKEKILRSGGKVMSLYKAIQELNDFKGKTVRLMK

Entity 15 polymer

描述 description	50S ribosomal protein L18
分子量 formula_weight	21766.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L18P

TQGPNYRIKFRRRREGKTDYYTRYTYVINNAIRFVPRLTNKYVIVSVSKFDQKGDIMIAYAHSIELVKKYGWKGDTNNTPAAYLTGYLAGLRAVKSGVKAAVSDIGLFVPVKGGRIFAVIKGAIDAGLKVPVGDLGKLKDRVNGSHISAYAQKLKNENQELYNKLFSSYIQRGLDPVLLPQHFEEVLNKIKEN

Entity 16 polymer

描述 description	50S ribosomal protein L19e
分子量 formula_weight	17108.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L19E

PEFQLQRRLAADIAGVGLNNIKFNPERLEEVEEALTREDIKKLIKERAVIVNPKRGISSGRLKERKHKRRSKGEGRKHGSRKGKSGARTGDKEIWINKIRKIRRYIRWLRDNNVIDKHTYRLLYKRAKGNYFKNLSDVKSYLRQ

Entity 17 polymer

描述 description	50S ribosomal protein L22
分子量 formula_weight	17360.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L22P

YPILSIPEDKLAKAVIKNIDASVRDLYNVCKAIRGMNLKEAREFLNNVLEEKEALPFWRYSHGTSHRSNISRKWKVKSGRYPKKAIKYVLKLLDNAENNANSKGLDIDNLKIVHIAAHKGLVLKRYMTRAFGRSTRKYKYLSHIEVILGE

Entity 18 polymer

描述 description	50S ribosomal protein L23
分子量 formula_weight	9262.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L23P

IIKEFLATEKAVKLIESQNTLTIIVNRNTTKKDIKNEVEKLFSVKVEKINTLITPKGEKKAYVKLKQEYKASDVAHKLGML

Entity 19 polymer

描述 description	50S ribosomal protein L24e
分子量 formula_weight	6338.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L24E

RQCSFCGKDILPGTGLMYVRNDGSLLWFCSSKCRKSMLKLHRDPKKLKWTKSYL

Entity 20 polymer

描述 description	50S ribosomal protein L24
分子量 formula_weight	14130.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L24P

MISSKPSKQRKLVYNLPNHLRYKLLTARLSEDLEKQYGIKRISIRKGDSVKLMRGSQVGYEGKVVEVDRKRGRVAIEGLTKKKADGTPVYVWVHASKVIITKLDTGDKERMDAIERKRKMRE

Entity 21 polymer

描述 description	50S ribosomal protein L29
分子量 formula_weight	7378.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L29P

PLEVDELRKMDLKQLKERLNELEMQLLKLRVESRMGTLKNTASIKNTRKDIARILTVIGEKSK

Entity 22 polymer

描述 description	50S ribosomal protein L30e
分子量 formula_weight	10391.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L30E

FEGELKTLLRSGKVILGTRKTLKLLKTGKVKGVVVSSTLRQDLKDDIMTFSKFSDIPIYLYKGSGYELGTLCGKPFMVSVIGIVDEGESKILEF

Entity 23 polymer

描述 description	50S ribosomal protein L30
分子量 formula_weight	17819.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L30P

MNELIGVIRIRGWAATPWYIQDTLSMLRLKNAFNAMIYPKDSSIQGMLNLVSSYVTWGELNDEGLRLLVSKLEISRGKKVNEEYVKDKLNVDFQSFVESIKDGKLRLNKLDEIFSLPIRLHPPKGGFKGKVSRPFRAGGEFGYRGDKINELIRRM

Entity 24 polymer

描述 description	50S ribosomal protein L31e
分子量 formula_weight	8935.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L31E

KPEKENFEMVINFRRVIMGRKTTRTKRAIKYVRYMVKRHFGAEKVIIDPLLAKAITMNGRDKIVRRVRIAVKRIG

Entity 25 polymer

描述 description	50S ribosomal protein L32e
分子量 formula_weight	14328.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L32E

KPAKKNMKSPGKAIKFLRFDWDKYYRIGRQERWRKPRGIDNAIRLELKGYQPKVKIGYRTDKQIRGLHPSGLRPILVKSVKDLEAFAKGKQDVIIIISSTIGLRKRIELIKKAEELGLKIANR

Entity 26 polymer

描述 description	50S ribosomal protein L34e
分子量 formula_weight	8993.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L34E

SPQQRSGSFRKVFVKLPSGKSTIHYERRKDNIARCGMCKKPLNGVKNNYTYKYSKTEKRPERVYGGYLCHKCLESLI

Entity 27 polymer

描述 description	50S ribosomal protein L37Ae
分子量 formula_weight	7536.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L37A

TGIAGRFGARYGSSTRKKWKEVMERRYMPHQCPYCKTTGKVVRIASGIWYCRKCETKWAGLAYTP

Entity 28 polymer

描述 description	50S ribosomal protein L37e
分子量 formula_weight	6274.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L37E

MKGTPSFGKMNKTPTHVRCRRCGRNSFNARKGYCAACGFGRSKKIRRYSWQNKK

Entity 29 polymer

描述 description	50S ribosomal protein L39e
分子量 formula_weight	5861.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L39E

KHKSLGKKLRLGKALKRNSPIPAWVIIKTQAEIRFNPLRRNWRRNNLKV

Entity 30 polymer

描述 description	50S ribosomal protein L40E
分子量 formula_weight	6336.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L40E

MPLTDPVKLQIVQQRVFLKKICRDCGALNSIRATKCRRCHSSNLRAKKKELPAKK

Entity 31 polymer

描述 description	50S ribosomal protein L44e
分子量 formula_weight	10806.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L44E

MKVPKVIKTYCPKCKTHTEHSVSLYKGGKRRSLAEGQRRYERKNVGYGSKRKPEQKRFAKTTKKQTLVLKCQKCSYTIVKEGMRLKKLELVE

Entity 32 polymer

描述 description	50S ribosomal protein L7Ae
分子量 formula_weight	13351.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L7A1,L7A2

PSYVKFEVPQELADKVLEAVKKAKDSGKIKKGTNETTKAVERSQAKLVVIAEDVQPEEIVAHLPLLCEEKKIPYVYVPSKKSLGEACGLQVAAASVALMDPGEAKDLVDEIVKRVNEIKGKSS

Entity 33 polymer

描述 description	50S ribosomal protein L15
分子量 formula_weight	16476.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L15P

MVVRKEKKSRKYRGYRTHGWGTKGQHRDRGAQGGRQIGMHKEKWSWTVKFGEGWYGKHGFRNPTSKLVNAIGLRKLQEYIDNDKIKIEEENGKKVVDLAKYGYDKLLGGGNLRLPLVIKVAKATEKAKERVKEIGGEIILTSSE

Entity 34 polymer

描述 description	50S ribosomal protein L21e
分子量 formula_weight	11155.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L21E

VARSKGYRSKTRKLLEKKVREKGAIPKLSLLMHDYSQGEYVVVKINPSIHKGMPHRRYHGKVGLVVGKRGKAYEVKVNIGDKERVLIVRPEHLIPFN

Entity 35 polymer

描述 description	DUF2280 domain-containing protein
分子量 formula_weight	11662.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L45A

MKVVKYVLTAKIDYDGKEIEIKRSFRHRKNIFEFLKNADSNLKGALSEYAKQNKVKSSDLPLNEFITCGEIEEEQGVMRTECKGKEKVSVKIEVQTQKIAK

Entity 36 polymer

描述 description	Conserved protein
分子量 formula_weight	8320.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L46A

MLKHQKYVYVDIGDQKKFLKVRFLKSRSENNNPEAYVILDKISIKLPRNAKVIKYEDLPAEVKDKLKLKL

Entity 37 polymer

描述 description	50S ribosomal protein L47A
分子量 formula_weight	9276.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L47A

MKISNVESKYSMKVRGQELLIEEARNEKGEKIIIFQSLTSAKLLNNEEWKENTTDAKNVEKREDLPQDVRKITGKILSLI

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	triclinic

分辨率 resolution	4.94 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	617754

fit	/app/data/saxs_computation/hk/8hkv/8hkv.fit
pddf	/app/data/pr_computation/hk/8hkv/8hkv.pr.out
profile	/app/data/saxs_computation/hk/8hkv/8hkv.dat

Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (37)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 polymer

聚合体 Polymer

Entity 32 polymer

聚合体 Polymer

Entity 33 polymer

聚合体 Polymer

Entity 34 polymer

聚合体 Polymer

Entity 35 polymer